CA2720526C - Cellular production of glucaric acid - Google Patents

Cellular production of glucaric acid Download PDF

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Publication number
CA2720526C
CA2720526C CA2720526A CA2720526A CA2720526C CA 2720526 C CA2720526 C CA 2720526C CA 2720526 A CA2720526 A CA 2720526A CA 2720526 A CA2720526 A CA 2720526A CA 2720526 C CA2720526 C CA 2720526C
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Prior art keywords
cell
myo
inositol
gene
glucaric acid
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CA2720526A
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English (en)
French (fr)
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CA2720526A1 (en
Inventor
Tae Seok Moon
Sang-Hwal Yoon
Kristala Lanett Jones Prather
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Massachusetts Institute of Technology
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Massachusetts Institute of Technology
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/58—Aldonic, ketoaldonic or saccharic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01203—Uronate dehydrogenase (1.1.1.203)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/99—Miscellaneous (1.13.99)
    • C12Y113/99001—Inositol oxygenase (1.13.99.1), i.e. myo-inositol oxygenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y505/00—Intramolecular lyases (5.5)
    • C12Y505/01—Intramolecular lyases (5.5.1)
    • C12Y505/01004—Inositol-3-phosphate synthase (5.5.1.4)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
CA2720526A 2008-04-04 2009-04-03 Cellular production of glucaric acid Active CA2720526C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US4250208P 2008-04-04 2008-04-04
US61/042,502 2008-04-04
PCT/US2009/002111 WO2009145838A2 (en) 2008-04-04 2009-04-03 Cellular production of glucaric acid

Publications (2)

Publication Number Publication Date
CA2720526A1 CA2720526A1 (en) 2009-12-03
CA2720526C true CA2720526C (en) 2017-11-28

Family

ID=41377836

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2720526A Active CA2720526C (en) 2008-04-04 2009-04-03 Cellular production of glucaric acid

Country Status (8)

Country Link
US (2) US8835147B2 (https=)
EP (1) EP2274417B1 (https=)
JP (2) JP6196016B2 (https=)
KR (1) KR101625443B1 (https=)
CN (1) CN102066552B (https=)
CA (1) CA2720526C (https=)
DK (1) DK2274417T3 (https=)
WO (1) WO2009145838A2 (https=)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
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FI20086236A0 (fi) * 2008-12-23 2008-12-23 Valtion Teknillinen Heksuronihapon konversio heksarihapoksi
EP2921558B1 (en) 2011-11-14 2017-10-25 Asahi Kasei Kabushiki Kaisha Method for producing myo-inositol and myo-inositol derivative
CN104080918B (zh) * 2012-02-24 2017-02-22 旭化成株式会社 葡萄糖二酸的制造方法
WO2013173711A1 (en) * 2012-05-18 2013-11-21 Novozymes A/S Bacterial mutants with improved transformation efficiency
CN105026548B (zh) * 2012-06-04 2019-06-18 盐水港精糖株式会社 D-葡萄糖二酸生产菌及d-葡萄糖二酸的制造方法
US9528133B2 (en) 2012-09-21 2016-12-27 Synthetic Genomics, Inc. Compositions and methods for producing chemicals and derivatives thereof
WO2014047510A1 (en) * 2012-09-21 2014-03-27 Synthetic Genomics, Inc. Compositions and methods for producing chemicals and derivatives thereof
CA2943348A1 (en) * 2014-03-21 2015-09-24 Synthetic Genomics, Inc. Compositions and methods for producing chemicals and derivatives thereof
WO2015191638A1 (en) * 2014-06-09 2015-12-17 Massachusetts Institute Of Technology Dynamic knockdown of central metabolism for redirecting glucose-6-phosphate fluxes
CN104312934A (zh) * 2014-10-22 2015-01-28 江南大学 一种构建重组酵母生物合成葡萄糖醛酸的方法
CN104312935B (zh) * 2014-10-22 2018-07-06 江南大学 一种构建重组酵母发酵生产葡萄糖二酸的方法
US10752888B2 (en) 2016-06-30 2020-08-25 Cj Cheiljedang Corporation Method for enzymatically preparing highly concentrated myo-inositol
CN106148292B (zh) * 2016-07-06 2019-05-17 江南大学 一种肌醇加氧酶突变体及其应用
WO2018067458A1 (en) * 2016-10-03 2018-04-12 The Regents Of The University Of California Engineered microorganisms for production of commodity chemicals and cellular biomass
CN106929459B (zh) * 2017-03-30 2026-03-20 华南理工大学 一种重组大肠杆菌及其构建方法与通过代谢工程生产葡萄糖二酸的方法
FR3067350B1 (fr) 2017-06-12 2020-12-11 Seprosys Procede d'obtention d'une solution d'acide glucarique libre et solution obtenue
BR112019027341A2 (pt) 2017-06-22 2020-07-07 Archer Daniels Midland Company processo para produzir ésteres de fdca e processo para produzir ésteres de fdca a partir de dextrose
CN107365806B (zh) * 2017-08-02 2020-04-03 南京师范大学 一种葡萄糖二酸的制备方法及其制备中的糖醛酸脱氢酶-nadh氧化酶和应用
EP3969600B1 (en) * 2019-05-17 2024-08-07 The Governing Council of the University of Toronto Enzymatic production of glucaric acid from glucuronic acid
CN110846436B (zh) * 2019-12-25 2021-09-28 江南大学 一种快速鉴别Ale和Lager型啤酒酵母的方法
CN110951794B (zh) * 2019-12-31 2022-03-25 江南大学 一种提高酿酒酵母工程菌生产葡萄糖二酸的发酵方法
CN111019852B (zh) * 2019-12-31 2021-11-23 江南大学 一种提高酿酒酵母工程菌株合成葡萄糖二酸效率的方法
KR102649855B1 (ko) * 2020-09-15 2024-03-22 경북대학교 산학협력단 갈락투론산을 발효하여 뮤식산을 생산하는 신규 효모 균주
EP4314311A4 (en) * 2021-03-26 2025-12-24 Archer Daniels Midland Co D-GLUCARATE DEHYDRATASE THERMOSTABLE WHICH IS RESISTANT TO INHIBITION BY TARTRATE SEQUENCE SUBMISSION
KR102626750B1 (ko) * 2021-12-27 2024-01-19 한국프라임제약주식회사 키로이노시톨 생성능이 우수한 락티카제이 바실러스 카제이 WiKim0135 균주 및 이의 혈당강하 용도
CN115820761A (zh) * 2022-11-28 2023-03-21 南京工业大学 一种生物法催化合成d-葡萄糖醛酸的方法
WO2024217529A1 (zh) * 2023-04-21 2024-10-24 西湖大学 一种新型的葡萄糖二酸生物合成路径及发酵生产葡萄糖二酸的方法
CN119752971A (zh) * 2025-01-10 2025-04-04 福州大学 一种利用d-木糖和甲醇生物合成d-葡萄糖二酸的方法

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DE19604798A1 (de) * 1996-02-09 1997-08-14 Herbstreith & Fox Kg Pektin Fa Herstellung von L-Ascorbinsäure
US7326549B2 (en) * 2001-03-19 2008-02-05 Cargill, Incorporated Myo-inositol oxygenases

Also Published As

Publication number Publication date
KR101625443B1 (ko) 2016-05-30
CN102066552B (zh) 2014-03-12
EP2274417A2 (en) 2011-01-19
JP2017018140A (ja) 2017-01-26
CN102066552A (zh) 2011-05-18
EP2274417B1 (en) 2013-07-24
KR20110027652A (ko) 2011-03-16
WO2009145838A3 (en) 2010-02-18
EP2274417A4 (en) 2012-05-02
JP2011516063A (ja) 2011-05-26
US8835147B2 (en) 2014-09-16
WO2009145838A2 (en) 2009-12-03
DK2274417T3 (da) 2013-10-28
US20110124065A1 (en) 2011-05-26
JP6196016B2 (ja) 2017-09-13
CA2720526A1 (en) 2009-12-03
BRPI0911171A2 (pt) 2015-08-04
US20150093794A1 (en) 2015-04-02

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