CA2697621C - Method of efficiently establishing induced pluripotent stem cells - Google Patents

Method of efficiently establishing induced pluripotent stem cells Download PDF

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CA2697621C
CA2697621C CA2697621A CA2697621A CA2697621C CA 2697621 C CA2697621 C CA 2697621C CA 2697621 A CA2697621 A CA 2697621A CA 2697621 A CA2697621 A CA 2697621A CA 2697621 C CA2697621 C CA 2697621C
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cells
oct3
cell
myc
sox2
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CA2697621A1 (en
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Shinya Yamanaka
Yoshinori Yoshida
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Kyoto University NUC
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0696Artificially induced pluripotent stem cells, e.g. iPS
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068General culture methods using substrates
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0607Non-embryonic pluripotent stem cells, e.g. MASC
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60Transcription factors
    • C12N2501/602Sox-2
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60Transcription factors
    • C12N2501/603Oct-3/4
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60Transcription factors
    • C12N2501/606Transcription factors c-Myc
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    • C12N2510/00Genetically modified cells

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  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Transplantation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Virology (AREA)
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  • Physics & Mathematics (AREA)
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CA2697621A 2008-07-30 2009-07-30 Method of efficiently establishing induced pluripotent stem cells Active CA2697621C (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US8484208P 2008-07-30 2008-07-30
US61/084,842 2008-07-30
US14117708P 2008-12-29 2008-12-29
US61/141,177 2008-12-29
US20393108P 2008-12-30 2008-12-30
US61/203,931 2008-12-30
PCT/JP2009/063906 WO2010013845A1 (en) 2008-07-30 2009-07-30 Method of efficiently establishing induced pluripotent stem cells

Publications (2)

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CA2697621A1 CA2697621A1 (en) 2010-02-04
CA2697621C true CA2697621C (en) 2017-01-17

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US (1) US9528092B2 (OSRAM)
EP (1) EP2307539B1 (OSRAM)
JP (1) JP5562231B2 (OSRAM)
KR (1) KR101685209B1 (OSRAM)
CN (2) CN101848994A (OSRAM)
CA (1) CA2697621C (OSRAM)
WO (1) WO2010013845A1 (OSRAM)

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Publication number Publication date
KR20110036523A (ko) 2011-04-07
CN101848994A (zh) 2010-09-29
EP2307539A1 (en) 2011-04-13
US20110039338A1 (en) 2011-02-17
US9528092B2 (en) 2016-12-27
WO2010013845A1 (en) 2010-02-04
EP2307539A4 (en) 2012-10-24
JP2011529329A (ja) 2011-12-08
CA2697621A1 (en) 2010-02-04
EP2307539B1 (en) 2014-11-19
CN107012123A (zh) 2017-08-04
JP5562231B2 (ja) 2014-07-30
KR101685209B1 (ko) 2016-12-09

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