CA2670003A1 - Ablation genetique du gene prp, cellules utilisant un piege a promoteur cible, strategie pour la production de proteines recombinantes exemptes de serum en tant que produits therapeutiques - Google Patents

Ablation genetique du gene prp, cellules utilisant un piege a promoteur cible, strategie pour la production de proteines recombinantes exemptes de serum en tant que produits therapeutiques Download PDF

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Publication number
CA2670003A1
CA2670003A1 CA002670003A CA2670003A CA2670003A1 CA 2670003 A1 CA2670003 A1 CA 2670003A1 CA 002670003 A CA002670003 A CA 002670003A CA 2670003 A CA2670003 A CA 2670003A CA 2670003 A1 CA2670003 A1 CA 2670003A1
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Canada
Prior art keywords
prp
cells
human
cell line
free
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002670003A
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English (en)
Inventor
Carola Schroeder
Elisabeth Casademunt
Kim Bjoernstrup
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Octagene GmbH
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Individual
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Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2670003A1 publication Critical patent/CA2670003A1/fr
Abandoned legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0684Cells of the urinary tract or kidneys
    • C12N5/0686Kidney cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10Cells modified by introduction of foreign genetic material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/90Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/92Medium free of human- or animal-derived components
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/90Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/95Protein-free medium and culture conditions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00Genetically modified cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00Genetically modified cells
    • C12N2510/02Cells for production

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Urology & Nephrology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA002670003A 2006-11-20 2007-11-20 Ablation genetique du gene prp, cellules utilisant un piege a promoteur cible, strategie pour la production de proteines recombinantes exemptes de serum en tant que produits therapeutiques Abandoned CA2670003A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP06124427.3 2006-11-20
EP06124427 2006-11-20
PCT/EP2007/062597 WO2008061995A2 (fr) 2006-11-20 2007-11-20 Ablation génétique du gène prp, cellules utilisant un piège à promoteur ciblé, stratégie pour la production de protéines recombinantes exemptes de sérum en tant que produits thérapeutiques

Publications (1)

Publication Number Publication Date
CA2670003A1 true CA2670003A1 (fr) 2008-05-29

Family

ID=37685785

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002670003A Abandoned CA2670003A1 (fr) 2006-11-20 2007-11-20 Ablation genetique du gene prp, cellules utilisant un piege a promoteur cible, strategie pour la production de proteines recombinantes exemptes de serum en tant que produits therapeutiques

Country Status (12)

Country Link
EP (1) EP2084269A2 (fr)
KR (1) KR20090106474A (fr)
CN (1) CN101605891A (fr)
AU (1) AU2007324530A1 (fr)
BR (1) BRPI0718989A2 (fr)
CA (1) CA2670003A1 (fr)
IL (1) IL198770A0 (fr)
MX (1) MX2009005277A (fr)
NO (1) NO20091875L (fr)
RU (1) RU2009123458A (fr)
WO (1) WO2008061995A2 (fr)
ZA (1) ZA200903429B (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG185511A1 (en) * 2010-05-12 2012-12-28 Centocor Ortho Biotech Inc Differentiation of human embryonic stem cells
CN102277379B (zh) * 2011-08-18 2013-07-24 中国科学院遗传与发育生物学研究所 表达凝血因子viii的表达载体及其应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5464764A (en) * 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
DE69233477T2 (de) * 1991-11-14 2006-02-16 Prionics Ag Transgene nicht-menschliche tiere ohne prionprotein
BR9916475A (pt) * 1998-12-22 2001-12-04 Univ California Células somáticas com gene prp removido emétodos de uso
EP1282721A4 (fr) * 2000-03-24 2005-09-07 Univ Massachusetts Ongules transgeniques depourvus de prions
US7265262B2 (en) * 2001-03-21 2007-09-04 Roslin Institute (Edinburgh) Telomerizing nuclear donor cells and improving the efficiency on nuclear transfer

Also Published As

Publication number Publication date
BRPI0718989A2 (pt) 2014-02-11
WO2008061995A3 (fr) 2008-07-10
MX2009005277A (es) 2009-05-28
EP2084269A2 (fr) 2009-08-05
CN101605891A (zh) 2009-12-16
KR20090106474A (ko) 2009-10-09
RU2009123458A (ru) 2010-12-27
NO20091875L (no) 2009-07-28
WO2008061995A2 (fr) 2008-05-29
ZA200903429B (en) 2010-04-28
AU2007324530A1 (en) 2008-05-29
IL198770A0 (en) 2011-08-01

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Legal Events

Date Code Title Description
FZDE Discontinued