CA2656192C - Detectable nucleic acid tag - Google Patents

Detectable nucleic acid tag Download PDF

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Publication number
CA2656192C
CA2656192C CA2656192A CA2656192A CA2656192C CA 2656192 C CA2656192 C CA 2656192C CA 2656192 A CA2656192 A CA 2656192A CA 2656192 A CA2656192 A CA 2656192A CA 2656192 C CA2656192 C CA 2656192C
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CA
Canada
Prior art keywords
nucleic acid
protein
interest
binding domain
fusion protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2656192A
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English (en)
French (fr)
Other versions
CA2656192A1 (en
Inventor
Pietro Ciceri
Jeremy Hunt
Jean-Michael A. Lelias
Mike Morrison
Daniel Treiber
Lisa M. Wodicka
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eurofins Discoverx Corp
Original Assignee
DiscoveRx Corp
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Publication date
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Publication of CA2656192A1 publication Critical patent/CA2656192A1/en
Application granted granted Critical
Publication of CA2656192C publication Critical patent/CA2656192C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2522/00—Reaction characterised by the use of non-enzymatic proteins
    • C12Q2522/10—Nucleic acid binding proteins
    • C12Q2522/101—Single or double stranded nucleic acid binding proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/101—Nucleic acid detection characterized by the use of physical, structural and functional properties radioactivity, e.g. radioactive labels
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/113—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being electroactive, e.g. redox labels
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Enzymes And Modification Thereof (AREA)
CA2656192A 2006-06-30 2007-06-29 Detectable nucleic acid tag Active CA2656192C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US80642206P 2006-06-30 2006-06-30
US60/806,422 2006-06-30
PCT/US2007/015089 WO2008005310A2 (en) 2006-06-30 2007-06-29 Detectable nucleic acid tag

Publications (2)

Publication Number Publication Date
CA2656192A1 CA2656192A1 (en) 2008-01-10
CA2656192C true CA2656192C (en) 2016-05-03

Family

ID=38895122

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2656192A Active CA2656192C (en) 2006-06-30 2007-06-29 Detectable nucleic acid tag

Country Status (10)

Country Link
US (2) US9110054B2 (enExample)
EP (2) EP2044219B1 (enExample)
JP (2) JP5415264B2 (enExample)
KR (1) KR20090034930A (enExample)
CN (1) CN101512016B (enExample)
AU (1) AU2007269786A1 (enExample)
CA (1) CA2656192C (enExample)
IL (1) IL196258A0 (enExample)
MX (1) MX2009000044A (enExample)
WO (1) WO2008005310A2 (enExample)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT2268623E (pt) 2008-03-17 2015-09-17 Ambit Biosciences Corp Derivados de quinazolina como moduladores de cinase raf e métodos para utilização dos mesmos
CN102395576A (zh) 2009-02-27 2012-03-28 埃姆比特生物科学公司 调控jak激酶的喹唑啉衍生物和其使用方法
WO2010124157A1 (en) * 2009-04-24 2010-10-28 Ambit Biosciences Corporation Cellular assay employing detectable protein
KR20120059558A (ko) 2009-08-19 2012-06-08 암비트 바이오사이언시즈 코포레이션 바이아릴 화합물 및 이의 사용 방법
US20120214208A1 (en) * 2009-09-16 2012-08-23 Massey University Fusion polypeptides and uses thereof
WO2011056764A1 (en) 2009-11-05 2011-05-12 Ambit Biosciences Corp. Isotopically enriched or fluorinated imidazo[2,1-b][1,3]benzothiazoles
US9296722B2 (en) 2010-05-27 2016-03-29 Ambit Biosciences Corporation Azolyl urea compounds and methods of use thereof
WO2011150201A2 (en) 2010-05-27 2011-12-01 Ambit Biosciences Corporation Azolyl amide compounds and methods of use thereof
WO2012030910A1 (en) 2010-09-01 2012-03-08 Ambit Biosciences Corporation 2-cycloquinazoline derivatives and methods of use thereof
US20130225614A1 (en) 2010-09-01 2013-08-29 Ambit Biosciences Corporation 4-azolylaminoquinazoline derivatives and methods of use thereof
EP2611448A1 (en) 2010-09-01 2013-07-10 Ambit Biosciences Corporation 7-cyclylquinazoline derivatives and methods of use thereof
JP5932794B2 (ja) 2010-09-01 2016-06-08 アムビト ビオスシエンセス コルポラチオン 光学活性のあるピラゾリルアミノキナゾリン及びその医薬組成物及び使用方法
US20130303533A1 (en) 2010-09-01 2013-11-14 Ambit Biosciences Corporation Azolopyridine and azolopyrimidine compounds and methods of use thereof
WO2012030894A1 (en) 2010-09-01 2012-03-08 Ambit Biosciences Corporation Thienopyridine and thienopyrimidine compounds and methods of use thereof
CA2810024A1 (en) 2010-09-01 2012-03-08 Ambit Biosciences Corporation Quinazoline compounds and methods of use thereof
WO2012030944A2 (en) 2010-09-01 2012-03-08 Ambit Biosciences Corporation Quinoline and isoquinoline compounds and methods of use thereof
RS54936B1 (sr) 2011-10-14 2016-11-30 Ambit Biosciences Corp Heterociklična jedinjenja i njihova upotreba kao modulatora receptora tirozin kinaza tipa iii
CN104156739B (zh) * 2013-05-14 2019-09-10 上海亨臻实业有限公司 电子标签亲和配体库及用途
GB201409282D0 (en) * 2014-05-23 2014-07-09 Univ Sydney Tech Sequencing process
WO2016008120A1 (zh) * 2014-07-16 2016-01-21 中国科学技术大学 基于核酸序列的蛋白质相互作用检测方法
WO2016028804A1 (en) 2014-08-21 2016-02-25 Discoverx Corporation Homogenous thermal shift ligand binding assay
US10829484B2 (en) 2015-07-28 2020-11-10 Plexxikon Inc. Compounds and methods for kinase modulation, and indications therefor
EP4354140A3 (en) 2016-02-26 2024-07-24 The Board of Trustees of the Leland Stanford Junior University Multiplexed single molecule rna visualization with a two-probe proximity ligation system
WO2018081113A1 (en) 2016-10-24 2018-05-03 Sawaya Sterling Concealing information present within nucleic acids
JP7105774B2 (ja) 2016-12-01 2022-07-25 イグナイタ インコーポレイテッド がん治療のための方法
CN112243463B (zh) 2018-04-09 2024-12-13 小利兰·斯坦福大学托管委员会 原位基因测序方法
US11208677B2 (en) 2018-06-07 2021-12-28 Amgen Inc. Detection assay for protein-polynucleotide conjugates
CN109709080B (zh) * 2018-09-28 2020-06-02 山东大学 一种光控示踪剂在探测蛋白质的寡聚状态中的应用
JP2022509850A (ja) * 2018-12-07 2022-01-24 オクタント，インク． タンパク質間相互作用スクリーニングのためのシステム
KR20220031070A (ko) * 2019-07-08 2022-03-11 인스크립타 인코포레이티드 Lexa-rad51 융합 단백질을 통한 증가된 핵산-가이드된 세포 편집
KR102319229B1 (ko) * 2020-03-03 2021-10-28 차의과학대학교 산학협력단 반복유산 진단을 위한 분석방법 및 키트
US11091524B1 (en) 2020-04-22 2021-08-17 Houston Gene Therapeutics Llc Compositions for treatment of vascular disease
EP4189085A4 (en) * 2020-07-28 2024-08-28 Protillion Biosciences, Inc. SYSTEMS AND METHODS FOR TESTING MULTIPLE POLYPEPTIDES
CN112126680B (zh) * 2020-09-27 2022-10-14 北京大学 基于荧光定量pcr筛选痕量内分泌干扰物的试剂盒及方法
CN116793750B (zh) * 2023-06-30 2024-09-20 宁波大学 一种高细菌检测特异性的atp荧光检测拭子及其使用方法
WO2025209553A1 (en) * 2024-04-02 2025-10-09 Ningbo Leadart Biotechnologies Co., Ltd. System and method for detecting the interaction between reactive probes and proteins

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100566859B1 (ko) 1997-01-21 2006-04-03 제너럴 하스피톨 코포레이션 Ｒｎａ-단백질 융합물을 이용한 단백질의 선별
US6261804B1 (en) 1997-01-21 2001-07-17 The General Hospital Corporation Selection of proteins using RNA-protein fusions
GB9724829D0 (en) 1997-11-21 1998-01-21 Muller Rolf Transcription factor
US20030027214A1 (en) * 1999-02-17 2003-02-06 Kamb Carl Alexander Methods for substrate-ligand interaction screening
AU3117101A (en) * 2000-01-28 2001-08-07 Scripps Research Inst Synthetic internal ribosome entry sites and methods of identifying same
EP1138781A3 (en) * 2000-03-31 2004-07-14 Health Research, Inc. Method for Quantifying DNA binding activity of DNA binding proteins
DE60104019T2 (de) * 2000-12-18 2005-07-14 National Research Council Of Canada, Ottawa Zusammensetzungen, Verfahren und Reagentiensätze zur Erkennung von Protein-Protein Wechselwirkung störenden Substanzen
EP1410023B1 (en) 2001-04-10 2006-06-21 Ecole Polytechnique Fédérale de Lausanne Methods using o6-alkylguanine-dna alkyltransferases
JP2005521409A (ja) 2002-03-29 2005-07-21 ニューゲン テクノロジーズ， インコーポレイテッド 単一プライマー等温核酸増幅−増強型被分析物検出および定量
US20030186221A1 (en) 2002-04-02 2003-10-02 Lockhart David J. Phage display affinity filter and forward screen
GB0212652D0 (en) * 2002-05-31 2002-07-10 Univ Bristol Screening Assay
WO2004035732A2 (en) * 2002-08-29 2004-04-29 Five Prime Therapeutics, Inc. Human polypeptides encoded by polynucleotides and methods of their use
US20050015337A1 (en) 2002-09-17 2005-01-20 Washington Keith Anthony Address billing system
DK1644513T3 (da) 2003-06-20 2011-10-17 Discoverx Corp Assay til detektion af proteinbinding
WO2005067644A2 (en) 2004-01-07 2005-07-28 Ambit Biosciences Corporation Conjugated small molecules

Also Published As

Publication number Publication date
CA2656192A1 (en) 2008-01-10
JP6038759B2 (ja) 2016-12-07
JP2014057594A (ja) 2014-04-03
WO2008005310A3 (en) 2008-08-21
MX2009000044A (es) 2009-01-23
IL196258A0 (en) 2011-08-01
EP2405272A1 (en) 2012-01-11
CN101512016B (zh) 2016-08-03
US10324092B2 (en) 2019-06-18
WO2008005310A2 (en) 2008-01-10
AU2007269786A1 (en) 2008-01-10
KR20090034930A (ko) 2009-04-08
EP2044219A2 (en) 2009-04-08
US9110054B2 (en) 2015-08-18
US20150377886A1 (en) 2015-12-31
JP2009542211A (ja) 2009-12-03
US20090053701A1 (en) 2009-02-26
JP5415264B2 (ja) 2014-02-12
CN101512016A (zh) 2009-08-19
EP2044219B1 (en) 2013-05-22
EP2405272B1 (en) 2015-01-14

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