CA2583735A1 - Orthogonal translation components for the in vivo incorporation of unnatural amino acids - Google Patents

Orthogonal translation components for the in vivo incorporation of unnatural amino acids Download PDF

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Publication number
CA2583735A1
CA2583735A1 CA002583735A CA2583735A CA2583735A1 CA 2583735 A1 CA2583735 A1 CA 2583735A1 CA 002583735 A CA002583735 A CA 002583735A CA 2583735 A CA2583735 A CA 2583735A CA 2583735 A1 CA2583735 A1 CA 2583735A1
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Prior art keywords
trna
amino acid
unnatural amino
translation system
providing
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Abandoned
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CA002583735A
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English (en)
French (fr)
Inventor
Peter Schultz
Lital Alfonta
Jonathan R. Chittuluru
Alexander Deiters
Dan Groff
Daniel Summerer
Meng-Lin Tsao
Jiangyun Wang
Ning Wu
Jianming Xie
Huaqiang Zeng
Mohammad R. Seyedsayamdost
James Turner
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Scripps Research Institute
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Publication of CA2583735A1 publication Critical patent/CA2583735A1/en
Abandoned legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93Ligases (6)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1058Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Ecology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CA002583735A 2004-10-27 2005-10-27 Orthogonal translation components for the in vivo incorporation of unnatural amino acids Abandoned CA2583735A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US62273804P 2004-10-27 2004-10-27
US60/622,738 2004-10-27
PCT/US2005/039210 WO2006110182A2 (en) 2004-10-27 2005-10-27 Orthogonal translation components for the in vivo incorporation of unnatural amino acids

Publications (1)

Publication Number Publication Date
CA2583735A1 true CA2583735A1 (en) 2006-10-19

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Family Applications (1)

Application Number Title Priority Date Filing Date
CA002583735A Abandoned CA2583735A1 (en) 2004-10-27 2005-10-27 Orthogonal translation components for the in vivo incorporation of unnatural amino acids

Country Status (10)

Country Link
US (2) US8216804B2 (https=)
EP (3) EP2380982B1 (https=)
JP (2) JP5135504B2 (https=)
CN (2) CN101048506B (https=)
AU (1) AU2005330514B2 (https=)
BR (1) BRPI0517381A (https=)
CA (1) CA2583735A1 (https=)
IL (2) IL182495A0 (https=)
MX (1) MX2007004892A (https=)
WO (1) WO2006110182A2 (https=)

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Also Published As

Publication number Publication date
AU2005330514B2 (en) 2011-05-12
EP2380982A1 (en) 2011-10-26
CN101048506A (zh) 2007-10-03
JP2008517631A (ja) 2008-05-29
EP1807515A4 (en) 2008-06-18
CN102827827A (zh) 2012-12-19
WO2006110182A3 (en) 2006-12-21
EP2383340B1 (en) 2014-07-16
EP1807515A2 (en) 2007-07-18
IL182495A0 (en) 2007-09-20
EP2383340A2 (en) 2011-11-02
AU2005330514A1 (en) 2006-10-19
EP2383340A3 (en) 2012-03-21
IL211229A0 (en) 2011-04-28
EP2380982B1 (en) 2014-07-16
JP2012254073A (ja) 2012-12-27
US20130244245A1 (en) 2013-09-19
CN101048506B (zh) 2013-06-26
MX2007004892A (es) 2007-08-16
BRPI0517381A (pt) 2008-10-07
JP5135504B2 (ja) 2013-02-06
WO2006110182A2 (en) 2006-10-19
US20080233611A1 (en) 2008-09-25
CN102827827B (zh) 2014-10-29
US8216804B2 (en) 2012-07-10
JP5539948B2 (ja) 2014-07-02
US9102932B2 (en) 2015-08-11

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