CA2577180A1 - Single protein production in living cells facilitated by a messenger rna interferase - Google Patents

Single protein production in living cells facilitated by a messenger rna interferase Download PDF

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Publication number
CA2577180A1
CA2577180A1 CA002577180A CA2577180A CA2577180A1 CA 2577180 A1 CA2577180 A1 CA 2577180A1 CA 002577180 A CA002577180 A CA 002577180A CA 2577180 A CA2577180 A CA 2577180A CA 2577180 A1 CA2577180 A1 CA 2577180A1
Authority
CA
Canada
Prior art keywords
mutated
acid sequence
nucleic acid
target protein
mrna interferase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002577180A
Other languages
English (en)
French (fr)
Inventor
Inouye Masayori
Junjie Zhang
Motoo Suzuki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Medicine and Dentistry of New Jersey
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2577180A1 publication Critical patent/CA2577180A1/en
Abandoned legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10Cells modified by introduction of foreign genetic material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases RNAses, DNAses
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/06Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA002577180A 2004-11-04 2005-11-04 Single protein production in living cells facilitated by a messenger rna interferase Abandoned CA2577180A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US62497604P 2004-11-04 2004-11-04
US60/624,976 2004-11-04
PCT/US2005/040107 WO2006055292A2 (en) 2004-11-04 2005-11-04 Single protein production in living cells facilitated by a messenger rna interferase

Publications (1)

Publication Number Publication Date
CA2577180A1 true CA2577180A1 (en) 2006-05-26

Family

ID=36407606

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002577180A Abandoned CA2577180A1 (en) 2004-11-04 2005-11-04 Single protein production in living cells facilitated by a messenger rna interferase

Country Status (6)

Country Link
EP (1) EP1812582A4 (ja)
JP (1) JP5013375B2 (ja)
KR (1) KR101064783B1 (ja)
CN (1) CN101052713B (ja)
CA (1) CA2577180A1 (ja)
WO (1) WO2006055292A2 (ja)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007020873A1 (ja) * 2005-08-16 2007-02-22 Takara Bio Inc. 免疫不全ウイルス感染症の治療または予防のための核酸
WO2010040134A2 (en) 2008-10-04 2010-04-08 University Of Medicine And Dentistry Of New Jersey Independently inducible system of gene expression
EP2848695A1 (en) * 2013-09-16 2015-03-18 Inria Institut National de Recherche en Informatique et en Automatique Method for producing metabolites, peptides and recombinant proteins
CN103484471B (zh) * 2013-09-30 2015-07-08 王悦 人表皮细胞生长因子核酸序列及大肠杆菌表达载体
KR101776368B1 (ko) * 2014-10-02 2017-09-07 서울시립대학교 산학협력단 전령 rna 나노입자 및 그 제조방법
TW202000240A (zh) * 2018-02-23 2020-01-01 日商盧卡科學股份有限公司 用於在粒線體內使蛋白質表現之核酸、封入前述核酸之脂質膜結構體及其等之用途

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5807718A (en) * 1994-12-02 1998-09-15 The Scripps Research Institute Enzymatic DNA molecules
EP2298869A1 (en) * 2003-06-13 2011-03-23 University Of Medicine And Dentistry Of New Jersey Recombinant protein production in the presence of mRNA interferase

Also Published As

Publication number Publication date
KR101064783B1 (ko) 2011-09-14
KR20080088350A (ko) 2008-10-02
CN101052713A (zh) 2007-10-10
WO2006055292A3 (en) 2006-08-03
JP5013375B2 (ja) 2012-08-29
CN101052713B (zh) 2011-01-26
WO2006055292A2 (en) 2006-05-26
EP1812582A2 (en) 2007-08-01
JP2008518623A (ja) 2008-06-05
EP1812582A4 (en) 2013-03-20

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued