CA2535922A1 - Methods of synthesizing polynucleotides using thermostable enzymes - Google Patents

Methods of synthesizing polynucleotides using thermostable enzymes Download PDF

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Publication number
CA2535922A1
CA2535922A1 CA002535922A CA2535922A CA2535922A1 CA 2535922 A1 CA2535922 A1 CA 2535922A1 CA 002535922 A CA002535922 A CA 002535922A CA 2535922 A CA2535922 A CA 2535922A CA 2535922 A1 CA2535922 A1 CA 2535922A1
Authority
CA
Canada
Prior art keywords
cell
polynucleotide
kit
cloning vector
reaction mixture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002535922A
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English (en)
French (fr)
Inventor
Marjorie F. Oleksiak
Douglas L. Crawford
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Missouri-Columbia
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2535922A1 publication Critical patent/CA2535922A1/en
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
CA002535922A 2003-09-23 2004-09-23 Methods of synthesizing polynucleotides using thermostable enzymes Abandoned CA2535922A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US50530003P 2003-09-23 2003-09-23
US60/505,300 2003-09-23
PCT/US2004/031267 WO2005030995A1 (en) 2003-09-23 2004-09-23 Methods of synthesizing polynucleotides using thermostable enzymes

Publications (1)

Publication Number Publication Date
CA2535922A1 true CA2535922A1 (en) 2005-04-07

Family

ID=34393006

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002535922A Abandoned CA2535922A1 (en) 2003-09-23 2004-09-23 Methods of synthesizing polynucleotides using thermostable enzymes

Country Status (7)

Country Link
US (2) US20050064490A1 (cg-RX-API-DMAC10.html)
EP (1) EP1664347A1 (cg-RX-API-DMAC10.html)
JP (1) JP2007506430A (cg-RX-API-DMAC10.html)
CN (1) CN1856579A (cg-RX-API-DMAC10.html)
AU (1) AU2004276790A1 (cg-RX-API-DMAC10.html)
CA (1) CA2535922A1 (cg-RX-API-DMAC10.html)
WO (1) WO2005030995A1 (cg-RX-API-DMAC10.html)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006512092A (ja) 2002-12-30 2006-04-13 ザ・リージェンツ・オブ・ジ・ユニバーシティ・オブ・カリフォルニア 病原体の検出および分析のための方法および装置
US7799553B2 (en) 2004-06-01 2010-09-21 The Regents Of The University Of California Microfabricated integrated DNA analysis system
WO2006032044A2 (en) 2004-09-15 2006-03-23 Microchip Biotechnologies, Inc. Microfluidic devices
JP5063616B2 (ja) 2006-02-03 2012-10-31 インテジェニックス インコーポレイテッド マイクロ流体デバイス
US7766033B2 (en) 2006-03-22 2010-08-03 The Regents Of The University Of California Multiplexed latching valves for microfluidic devices and processors
US8841116B2 (en) 2006-10-25 2014-09-23 The Regents Of The University Of California Inline-injection microdevice and microfabricated integrated DNA analysis system using same
KR20100028526A (ko) 2007-02-05 2010-03-12 마이크로칩 바이오테크놀로지스, 인크. 마이크로유체 및 나노유체 장치, 시스템 및 응용
WO2009015296A1 (en) 2007-07-24 2009-01-29 The Regents Of The University Of California Microfabricated dropley generator
EP2234916A4 (en) 2008-01-22 2016-08-10 Integenx Inc UNIVERSAL SPECIMEN PREPARATION SYSTEM AND ITS USE IN AN INTEGRATED ANALYSIS SYSTEM
KR20110111449A (ko) 2008-12-31 2011-10-11 인터젠엑스 인크. 미세유체 칩을 갖는 기구
US8685648B2 (en) * 2009-02-03 2014-04-01 Biohelix Corporation Endonuclease-enhanced helicase-dependent amplification
US8388908B2 (en) 2009-06-02 2013-03-05 Integenx Inc. Fluidic devices with diaphragm valves
US8584703B2 (en) 2009-12-01 2013-11-19 Integenx Inc. Device with diaphragm valve
US8512538B2 (en) 2010-05-28 2013-08-20 Integenx Inc. Capillary electrophoresis device
WO2012024658A2 (en) 2010-08-20 2012-02-23 IntegenX, Inc. Integrated analysis system
US8763642B2 (en) 2010-08-20 2014-07-01 Integenx Inc. Microfluidic devices with mechanically-sealed diaphragm valves
US8916358B2 (en) 2010-08-31 2014-12-23 Greenlight Biosciences, Inc. Methods for control of flux in metabolic pathways through protease manipulation
WO2015021058A2 (en) 2013-08-05 2015-02-12 Greenlight Biosciences, Inc. Engineered proteins with a protease cleavage site
RU2017137788A (ru) 2015-03-30 2019-04-30 Гринлайт Байосайенсис, Инк. Бесклеточное получение рибонуклеиновой кислоты
CN109477127A (zh) * 2016-03-14 2019-03-15 R基因股份有限公司 超热稳定的赖氨酸-突变型ssDNA/RNA连接酶
SG11201808721YA (en) * 2016-04-06 2018-11-29 Greenlight Biosciences Inc Cell-free production of ribonucleic acid
KR20190100386A (ko) 2017-01-06 2019-08-28 그린라이트 바이오사이언시스, 아이엔씨. 당의 무세포 생산
WO2019075167A1 (en) 2017-10-11 2019-04-18 Greenlight Biosciences, Inc. METHODS AND COMPOSITIONS FOR THE PRODUCTION OF NUCLEOSIDE TRIPHOSPHATE AND RIBONUCLEIC ACID
CN114875002B (zh) * 2021-07-01 2023-05-26 温州医科大学 一种精氨酸突变的dna/rna连接酶

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Publication number Priority date Publication date Assignee Title
US5374553A (en) * 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US5283173A (en) * 1990-01-24 1994-02-01 The Research Foundation Of State University Of New York System to detect protein-protein interactions
US5756334A (en) * 1990-04-26 1998-05-26 New England Biolabs, Inc. Thermostable DNA polymerase from 9°N-7 and methods for producing the same
US5352778A (en) * 1990-04-26 1994-10-04 New England Biolabs, Inc. Recombinant thermostable DNA polymerase from archaebacteria
ATE197718T1 (de) * 1990-09-28 2000-12-15 Hoffmann La Roche Gereinigte thermostabile nukleinsäurepolymerase aus thermosipho africanus
AU664050B2 (en) * 1991-12-18 1995-11-02 Becton Dickinson & Company Process for lysing mycobacteria
AU681650B2 (en) * 1993-03-24 1997-09-04 Molecular Biology Resources, Inc. Dinucleotide restriction endonuclease preparations and methods of use
US6100078A (en) * 1994-04-01 2000-08-08 Gen-Probe Incorporated Purified DNA polymerase from bacillus stearothermophilus ATCC 12980
US6306588B1 (en) * 1997-02-07 2001-10-23 Invitrogen Corporation Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof
WO1999005284A1 (fr) * 1997-07-25 1999-02-04 Agene Research Institute, Co., Ltd. GENE HUMAIN RecQ4 CODANT HELICASE
US20010018192A1 (en) * 1998-02-12 2001-08-30 Terstappen Leon W.M.M. Labeled cells for use as an internal functional control in rare cell detection assays
ATE362986T1 (de) * 1998-10-30 2007-06-15 Cornell Res Foundation Inc High fidelity thermostabile ligase und ihre verwendung
US6436677B1 (en) * 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription
US6323009B1 (en) * 2000-06-28 2001-11-27 Molecular Staging, Inc. Multiply-primed amplification of nucleic acid sequences
WO2002004022A1 (en) * 2000-07-12 2002-01-17 Invitrogen Corp High fidelity polymerases and uses thereof

Also Published As

Publication number Publication date
WO2005030995A1 (en) 2005-04-07
JP2007506430A (ja) 2007-03-22
US20050064490A1 (en) 2005-03-24
AU2004276790A1 (en) 2005-04-07
EP1664347A1 (en) 2006-06-07
US20060252083A1 (en) 2006-11-09
CN1856579A (zh) 2006-11-01

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