AU2004276790A1 - Methods of synthesizing polynucleotides using thermostable enzymes - Google Patents

Methods of synthesizing polynucleotides using thermostable enzymes Download PDF

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Publication number
AU2004276790A1
AU2004276790A1 AU2004276790A AU2004276790A AU2004276790A1 AU 2004276790 A1 AU2004276790 A1 AU 2004276790A1 AU 2004276790 A AU2004276790 A AU 2004276790A AU 2004276790 A AU2004276790 A AU 2004276790A AU 2004276790 A1 AU2004276790 A1 AU 2004276790A1
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AU
Australia
Prior art keywords
cell
polynucleotide
kit
cloning vector
target
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
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AU2004276790A
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English (en)
Inventor
Douglas L. Crawford
Marjorie F. Oleksiak
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Missouri-Columbia
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University of Missouri-Columbia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Missouri-Columbia filed Critical University of Missouri-Columbia
Publication of AU2004276790A1 publication Critical patent/AU2004276790A1/en
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
AU2004276790A 2003-09-23 2004-09-23 Methods of synthesizing polynucleotides using thermostable enzymes Abandoned AU2004276790A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US50530003P 2003-09-23 2003-09-23
US60/505,300 2003-09-23
PCT/US2004/031267 WO2005030995A1 (en) 2003-09-23 2004-09-23 Methods of synthesizing polynucleotides using thermostable enzymes

Publications (1)

Publication Number Publication Date
AU2004276790A1 true AU2004276790A1 (en) 2005-04-07

Family

ID=34393006

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2004276790A Abandoned AU2004276790A1 (en) 2003-09-23 2004-09-23 Methods of synthesizing polynucleotides using thermostable enzymes

Country Status (7)

Country Link
US (2) US20050064490A1 (cg-RX-API-DMAC10.html)
EP (1) EP1664347A1 (cg-RX-API-DMAC10.html)
JP (1) JP2007506430A (cg-RX-API-DMAC10.html)
CN (1) CN1856579A (cg-RX-API-DMAC10.html)
AU (1) AU2004276790A1 (cg-RX-API-DMAC10.html)
CA (1) CA2535922A1 (cg-RX-API-DMAC10.html)
WO (1) WO2005030995A1 (cg-RX-API-DMAC10.html)

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JP2006512092A (ja) 2002-12-30 2006-04-13 ザ・リージェンツ・オブ・ジ・ユニバーシティ・オブ・カリフォルニア 病原体の検出および分析のための方法および装置
US7799553B2 (en) 2004-06-01 2010-09-21 The Regents Of The University Of California Microfabricated integrated DNA analysis system
WO2006032044A2 (en) 2004-09-15 2006-03-23 Microchip Biotechnologies, Inc. Microfluidic devices
JP5063616B2 (ja) 2006-02-03 2012-10-31 インテジェニックス インコーポレイテッド マイクロ流体デバイス
US7766033B2 (en) 2006-03-22 2010-08-03 The Regents Of The University Of California Multiplexed latching valves for microfluidic devices and processors
US8841116B2 (en) 2006-10-25 2014-09-23 The Regents Of The University Of California Inline-injection microdevice and microfabricated integrated DNA analysis system using same
KR20100028526A (ko) 2007-02-05 2010-03-12 마이크로칩 바이오테크놀로지스, 인크. 마이크로유체 및 나노유체 장치, 시스템 및 응용
WO2009015296A1 (en) 2007-07-24 2009-01-29 The Regents Of The University Of California Microfabricated dropley generator
EP2234916A4 (en) 2008-01-22 2016-08-10 Integenx Inc UNIVERSAL SPECIMEN PREPARATION SYSTEM AND ITS USE IN AN INTEGRATED ANALYSIS SYSTEM
KR20110111449A (ko) 2008-12-31 2011-10-11 인터젠엑스 인크. 미세유체 칩을 갖는 기구
US8685648B2 (en) * 2009-02-03 2014-04-01 Biohelix Corporation Endonuclease-enhanced helicase-dependent amplification
US8388908B2 (en) 2009-06-02 2013-03-05 Integenx Inc. Fluidic devices with diaphragm valves
US8584703B2 (en) 2009-12-01 2013-11-19 Integenx Inc. Device with diaphragm valve
US8512538B2 (en) 2010-05-28 2013-08-20 Integenx Inc. Capillary electrophoresis device
WO2012024658A2 (en) 2010-08-20 2012-02-23 IntegenX, Inc. Integrated analysis system
US8763642B2 (en) 2010-08-20 2014-07-01 Integenx Inc. Microfluidic devices with mechanically-sealed diaphragm valves
US8916358B2 (en) 2010-08-31 2014-12-23 Greenlight Biosciences, Inc. Methods for control of flux in metabolic pathways through protease manipulation
WO2015021058A2 (en) 2013-08-05 2015-02-12 Greenlight Biosciences, Inc. Engineered proteins with a protease cleavage site
RU2017137788A (ru) 2015-03-30 2019-04-30 Гринлайт Байосайенсис, Инк. Бесклеточное получение рибонуклеиновой кислоты
CN109477127A (zh) * 2016-03-14 2019-03-15 R基因股份有限公司 超热稳定的赖氨酸-突变型ssDNA/RNA连接酶
SG11201808721YA (en) * 2016-04-06 2018-11-29 Greenlight Biosciences Inc Cell-free production of ribonucleic acid
KR20190100386A (ko) 2017-01-06 2019-08-28 그린라이트 바이오사이언시스, 아이엔씨. 당의 무세포 생산
WO2019075167A1 (en) 2017-10-11 2019-04-18 Greenlight Biosciences, Inc. METHODS AND COMPOSITIONS FOR THE PRODUCTION OF NUCLEOSIDE TRIPHOSPHATE AND RIBONUCLEIC ACID
CN114875002B (zh) * 2021-07-01 2023-05-26 温州医科大学 一种精氨酸突变的dna/rna连接酶

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US5374553A (en) * 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US5283173A (en) * 1990-01-24 1994-02-01 The Research Foundation Of State University Of New York System to detect protein-protein interactions
US5756334A (en) * 1990-04-26 1998-05-26 New England Biolabs, Inc. Thermostable DNA polymerase from 9°N-7 and methods for producing the same
US5352778A (en) * 1990-04-26 1994-10-04 New England Biolabs, Inc. Recombinant thermostable DNA polymerase from archaebacteria
ATE197718T1 (de) * 1990-09-28 2000-12-15 Hoffmann La Roche Gereinigte thermostabile nukleinsäurepolymerase aus thermosipho africanus
AU664050B2 (en) * 1991-12-18 1995-11-02 Becton Dickinson & Company Process for lysing mycobacteria
AU681650B2 (en) * 1993-03-24 1997-09-04 Molecular Biology Resources, Inc. Dinucleotide restriction endonuclease preparations and methods of use
US6100078A (en) * 1994-04-01 2000-08-08 Gen-Probe Incorporated Purified DNA polymerase from bacillus stearothermophilus ATCC 12980
US6306588B1 (en) * 1997-02-07 2001-10-23 Invitrogen Corporation Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof
WO1999005284A1 (fr) * 1997-07-25 1999-02-04 Agene Research Institute, Co., Ltd. GENE HUMAIN RecQ4 CODANT HELICASE
US20010018192A1 (en) * 1998-02-12 2001-08-30 Terstappen Leon W.M.M. Labeled cells for use as an internal functional control in rare cell detection assays
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US6436677B1 (en) * 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription
US6323009B1 (en) * 2000-06-28 2001-11-27 Molecular Staging, Inc. Multiply-primed amplification of nucleic acid sequences
WO2002004022A1 (en) * 2000-07-12 2002-01-17 Invitrogen Corp High fidelity polymerases and uses thereof

Also Published As

Publication number Publication date
WO2005030995A1 (en) 2005-04-07
JP2007506430A (ja) 2007-03-22
US20050064490A1 (en) 2005-03-24
EP1664347A1 (en) 2006-06-07
US20060252083A1 (en) 2006-11-09
CN1856579A (zh) 2006-11-01
CA2535922A1 (en) 2005-04-07

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Legal Events

Date Code Title Description
MK4 Application lapsed section 142(2)(d) - no continuation fee paid for the application