CA2489982C - Preparation of microbial oil-containing polyunsaturated fatty acids - Google Patents

Preparation of microbial oil-containing polyunsaturated fatty acids Download PDF

Info

Publication number
CA2489982C
CA2489982C CA2489982A CA2489982A CA2489982C CA 2489982 C CA2489982 C CA 2489982C CA 2489982 A CA2489982 A CA 2489982A CA 2489982 A CA2489982 A CA 2489982A CA 2489982 C CA2489982 C CA 2489982C
Authority
CA
Canada
Prior art keywords
oil
process according
fermentation
micro
carbon source
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2489982A
Other languages
English (en)
French (fr)
Other versions
CA2489982A1 (en
Inventor
Hugo Streekstra
Petrus Joseph Maria Brocken
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DSM IP Assets BV
Original Assignee
DSM IP Assets BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DSM IP Assets BV filed Critical DSM IP Assets BV
Publication of CA2489982A1 publication Critical patent/CA2489982A1/en
Application granted granted Critical
Publication of CA2489982C publication Critical patent/CA2489982C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS OR COOKING OILS
    • A23D9/00—Other edible oils or fats, e.g. shortenings or cooking oils
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS OR COOKING OILS
    • A23D9/00—Other edible oils or fats, e.g. shortenings or cooking oils
    • A23D9/02—Other edible oils or fats, e.g. shortenings or cooking oils characterised by the production or working-up
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/158—Fatty acids; Fats; Products containing oils or fats
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/115—Fatty acids or derivatives thereof; Fats or oils
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/115—Fatty acids or derivatives thereof; Fats or oils
    • A23L33/12—Fatty acids or derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/67—Vitamins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
    • A61K8/925—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of animal origin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9728—Fungi, e.g. yeasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/02—Nutrients, e.g. vitamins, minerals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/10—Production of fats or fatty oils from raw materials by extracting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/005—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor after treatment of microbial biomass not covered by C12N1/02 - C12N1/08
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6431—Linoleic acids [18:2[n-6]]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6434—Docosahexenoic acids [DHA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6481—Phosphoglycerides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Polymers & Plastics (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Food Science & Technology (AREA)
  • Mycology (AREA)
  • Epidemiology (AREA)
  • Birds (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Sustainable Development (AREA)
  • Animal Husbandry (AREA)
  • Dermatology (AREA)
  • Virology (AREA)
  • Botany (AREA)
  • Obesity (AREA)
  • Neurology (AREA)
  • Hematology (AREA)
  • Diabetes (AREA)
CA2489982A 2002-06-19 2003-06-20 Preparation of microbial oil-containing polyunsaturated fatty acids Expired - Lifetime CA2489982C (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
EP02254262.5 2002-06-19
EP02254262 2002-06-19
EP02258713 2002-12-18
EP02258713.3 2002-12-18
EP03251169.3 2003-02-26
EP03251169 2003-02-26
PCT/EP2003/006552 WO2004009827A2 (en) 2002-06-19 2003-06-20 Preparation of microbial oil containing polyunsaturated fatty acids

Publications (2)

Publication Number Publication Date
CA2489982A1 CA2489982A1 (en) 2004-01-29
CA2489982C true CA2489982C (en) 2019-09-10

Family

ID=30773251

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2489982A Expired - Lifetime CA2489982C (en) 2002-06-19 2003-06-20 Preparation of microbial oil-containing polyunsaturated fatty acids

Country Status (14)

Country Link
US (3) US7470527B2 (https=)
EP (1) EP2333093B1 (https=)
JP (5) JP2005530519A (https=)
KR (4) KR20140023448A (https=)
CN (3) CN112280807A (https=)
AU (1) AU2003281542A1 (https=)
BR (2) BR0311916A (https=)
CA (1) CA2489982C (https=)
DK (1) DK1513941T3 (https=)
EA (2) EA200901291A1 (https=)
ES (2) ES2567569T3 (https=)
IL (1) IL165529A (https=)
MX (2) MXPA04012915A (https=)
WO (1) WO2004009827A2 (https=)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MXPA04012915A (es) 2002-06-19 2005-03-31 Dsm Ip Assets Bv Preparacion de aceite microbiano.
ATE430802T1 (de) 2003-08-21 2009-05-15 Monsanto Technology Llc Fettsäuredesaturasen aus primula
EP2799553B1 (en) 2004-03-31 2020-07-29 Cargill, Incorporated Process for fermenting sugars containing oligomeric saccharides
BRPI0509944A (pt) 2004-04-16 2007-09-25 Monsanto Technology Llc expressão de dessaturases de ácido graxo em milho
TWI397582B (zh) 2004-11-04 2013-06-01 Monsanto Technology Llc 油組合物之製備方法
US7550286B2 (en) * 2004-11-04 2009-06-23 E. I. Du Pont De Nemours And Company Docosahexaenoic acid producing strains of Yarrowia lipolytica
AU2007226680B2 (en) 2006-03-10 2013-10-24 Monsanto Technology Llc Soybean seed and oil compositions and methods of making same
TW200806184A (en) * 2006-04-11 2008-02-01 Martek Biosciences Corp Food products comprising long chain polyunsaturated fatty acids and methods for preparing the same
JP5371750B2 (ja) * 2007-06-04 2013-12-18 国立大学法人北海道大学 微生物発酵によるｄｈａ含有リン脂質の製造方法
CN101874117A (zh) 2007-09-12 2010-10-27 马太克生物科学公司 生物油及其制备与应用
EP2316278B1 (en) * 2008-08-29 2013-04-10 Pharvis R&D Korea Co., Ltd. Method for producing fermented edible plants or edible animal/plants, fermented edible plants or edible animal/plants produced by same, and foods containing same
US8207363B2 (en) 2009-03-19 2012-06-26 Martek Biosciences Corporation Thraustochytrids, fatty acid compositions, and methods of making and uses thereof
US9480271B2 (en) 2009-09-15 2016-11-01 Monsanto Technology Llc Soybean seed and oil compositions and methods of making same
WO2011090493A1 (en) 2010-01-19 2011-07-28 Martek Biosciences Corporation Eicosapentaenoic acid-producing microorganisms, fatty acid compositions, and methods of making and uses thereof
EP2545159B1 (en) * 2010-03-11 2016-05-11 DSM IP Assets B.V. Yeast strains and their uses in the production of lipids
WO2011133610A1 (en) * 2010-04-22 2011-10-27 E. I. Du Pont De Nemours And Company Method for obtaining polyunsaturated fatty acid-containing compositions from microbial biomass
CA2802835A1 (en) * 2010-06-30 2012-01-05 Nippon Suisan Kaisha, Ltd. Process for production of useful substances
US8999663B2 (en) * 2011-02-11 2015-04-07 E L Du Pont De Nemours And Company Method for obtaining a lipid-containing composition from microbial biomass
CN103781887B (zh) 2011-06-27 2016-08-17 陶氏环球技术有限责任公司 基因工程化微生物油的介电流体
HK1198515A1 (en) 2011-07-21 2015-05-15 Dsm Ip Assets B.V. Fatty acid compositions
CN103131529B (zh) * 2011-11-23 2016-02-24 丰益(上海)生物技术研发中心有限公司 一种提取微生物油脂的方法
KR101984342B1 (ko) 2011-12-30 2019-05-30 다우 글로벌 테크놀로지스 엘엘씨 파네센 기재 올리고머를 갖는 유전 유체
BR112015012946B1 (pt) * 2012-12-10 2021-09-28 Cargill, Incorporated Processo de fermentação em lote para fermentar um hidrolisato de amido
WO2014209830A1 (en) * 2013-06-25 2014-12-31 Shell Oil Company Direct method of producing fatty acid esters from microbial biomass
EP2826384A1 (de) 2013-07-16 2015-01-21 Evonik Industries AG Verfahren zur Trocknung von Biomasse
EP3733857A3 (en) * 2013-12-04 2020-12-09 Nippon Suisan Kaisha, Ltd. Dihomo-gamma-linolenic acid-containing microbial oil and dihomo-gamma-linolenic acid-containing microbial biomass
FI3200604T4 (fi) 2014-10-02 2025-07-29 Evonik Operations Gmbh Menetelmä rehun valmistamiseksi
ES2870093T3 (es) 2014-10-02 2021-10-26 Evonik Operations Gmbh Biomasa que contiene PUFA con estabilidad celular elevada y su empleo para la producción de piensos
ES2873094T3 (es) * 2014-10-02 2021-11-03 Evonik Operations Gmbh Procedimiento para la producción de un pienso que contiene PUFAs mediante extrusión de una biomasa que contiene PUFAs de tipo Labyrinthulomycetes
CA2958439C (en) 2014-10-02 2022-09-20 Evonik Industries Ag Feedstuff of high abrasion resistance and good stability in water, containing pufas
SG10202001800WA (en) * 2015-08-25 2020-04-29 Dsm Ip Assets Bv Refined oil compositions and methods for making
KR102405390B1 (ko) 2016-07-13 2022-06-03 에보닉 오퍼레이션스 게엠베하 용해된 지질을 함유하는 바이오매스로부터 지질을 분리하는 방법
CN110462014A (zh) 2016-12-27 2019-11-15 赢创德固赛有限公司 从含有脂质的生物质中分离脂质的方法
KR20200039704A (ko) * 2017-08-07 2020-04-16 디에스엠 아이피 어셋츠 비.브이. 농축된 다중-불포화 지방산의 제조 방법
EP3470502A1 (en) 2017-10-13 2019-04-17 Evonik Degussa GmbH Method of separating lipids from a lysed lipids containing biomass
EP3527664A1 (en) 2018-02-15 2019-08-21 Evonik Degussa GmbH Method of isolating lipids from a lipids containing biomass
EP3775248A1 (en) * 2018-03-30 2021-02-17 DSM IP Assets B.V. Method of obtaining a microbial oil and a method of reducing emulsion by maintaining a low concentration of carbohydrate
WO2019219396A1 (en) 2018-05-15 2019-11-21 Evonik Operations Gmbh Method of isolating lipids from a lysed lipids containing biomass by emulsion inversion
DK3794097T3 (da) 2018-05-15 2024-12-16 Evonik Operations Gmbh Fremgangsmåde til isolering af lipider fra en lipid-indeholdende biomasse ved hjælp af hydrofob silica
CN108902364A (zh) * 2018-08-04 2018-11-30 望江县振兴植物油厂（普通合伙） 一种儿童专用调和油及其制备方法
CN113301812A (zh) * 2018-10-17 2021-08-24 完美日股份有限公司 用于食物产品的重组组分和组合物
CN110747239B (zh) * 2019-11-26 2021-06-22 瞿瀚鹏 富含Sn-2位ARA的微生物油脂及其制备方法和应用
CN111575110A (zh) * 2020-05-27 2020-08-25 嘉必优生物技术（武汉）股份有限公司 一种含有多不饱和脂肪酸的微生物油脂的纯化方法

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0716424B2 (ja) 1985-10-01 1995-03-01 ライオン株式会社 アラキドン酸含有脂質の製造方法
JPS6438007A (en) * 1987-04-28 1989-02-08 Lion Corp Skin external preparation
JP2723243B2 (ja) * 1988-02-25 1998-03-09 サントリー株式会社 高度不飽和脂肪酸添加動物飼料
JPH01228486A (ja) * 1988-03-09 1989-09-12 Suntory Ltd 奇数鎖高度不飽和脂肪酸及びこれを含有する脂質の製造方法
JPH0213388A (ja) * 1988-06-30 1990-01-17 Lion Corp 不飽和脂肪酸含有脂質の製造方法
JPH02142486A (ja) * 1988-11-25 1990-05-31 Lion Corp 不飽和脂肪酸含有脂質の製造方法
US5658767A (en) * 1991-01-24 1997-08-19 Martek Corporation Arachidonic acid and methods for the production and use thereof
PH11992043811B1 (en) 1991-01-24 2002-08-22 Martek Corp Arachidonic acid and methods for the production and use thereof
JP3354582B2 (ja) * 1991-09-30 2002-12-09 サントリー株式会社 オメガ９系高度不飽和脂肪酸およびこれを含有する脂質の製造方法
US5583019A (en) * 1995-01-24 1996-12-10 Omegatech Inc. Method for production of arachidonic acid
ATE328104T1 (de) * 1996-03-28 2006-06-15 Dsm Ip Assets Bv Mikrobielles polyungesättigte fettsäure enthaltendes öl und verfahren zur herstellung eines öls aus pasteurisierter und granulierter biomasse
JP4197744B2 (ja) * 1996-12-27 2008-12-17 サントリー株式会社 微生物培養用培地、並びに不飽和脂肪酸またはこれを含有する脂質の製造方法
JP2000069987A (ja) 1998-08-28 2000-03-07 Suntory Ltd アラキドン酸含有脂質並びにジホモ−γ−リノレン酸含有脂質の製造方法
CN1074289C (zh) * 1999-04-14 2001-11-07 内蒙古美乐绿色食品开发公司 具有抑制肿瘤和免疫调节功能的口服液
EP1178103A1 (en) 2000-08-02 2002-02-06 Dsm N.V. Purifying crude pufa oils
DE50113264D1 (de) * 2001-03-08 2007-12-27 Enthone System zur elektrodialytischen Regeneration eines stromlosen Badelektrolyten
CN1362522A (zh) * 2001-12-26 2002-08-07 武汉烯王生物工程有限公司 用离子束生物工程诱变菌生产含花生四烯酸油脂的方法
MXPA04012915A (es) * 2002-06-19 2005-03-31 Dsm Ip Assets Bv Preparacion de aceite microbiano.

Also Published As

Publication number Publication date
KR101208304B1 (ko) 2012-12-05
US7470527B2 (en) 2008-12-30
BR0311916A (pt) 2005-03-29
CA2489982A1 (en) 2004-01-29
ES2812475T3 (es) 2021-03-17
JP2005530519A (ja) 2005-10-13
MXPA04012915A (es) 2005-03-31
US20090053342A1 (en) 2009-02-26
JP2011024582A (ja) 2011-02-10
EA200901291A1 (ru) 2010-02-26
JP2016127837A (ja) 2016-07-14
WO2004009827A3 (en) 2004-04-15
CN101787379A (zh) 2010-07-28
KR20050021984A (ko) 2005-03-07
KR101288258B1 (ko) 2013-07-26
AU2003281542A8 (en) 2004-02-09
WO2004009827A2 (en) 2004-01-29
IL165529A (en) 2010-05-31
KR20110039504A (ko) 2011-04-18
KR20140023448A (ko) 2014-02-26
EP2333093A1 (en) 2011-06-15
US20140017742A1 (en) 2014-01-16
US20050202148A1 (en) 2005-09-15
JP6226601B2 (ja) 2017-11-08
AU2003281542A1 (en) 2004-02-09
EP2333093B1 (en) 2020-06-10
JP2009261397A (ja) 2009-11-12
DK1513941T3 (en) 2016-04-18
IL165529A0 (en) 2006-01-15
ES2567569T3 (es) 2016-04-25
US10633678B2 (en) 2020-04-28
JP2014039540A (ja) 2014-03-06
BRPI0311916B1 (pt) 2020-01-28
EA200500048A1 (ru) 2005-06-30
CN1662654A (zh) 2005-08-31
MX337781B (es) 2016-03-16
CN100575497C (zh) 2009-12-30
KR20120134152A (ko) 2012-12-11
EA013051B1 (ru) 2010-02-26
CN112280807A (zh) 2021-01-29

Similar Documents

Publication Publication Date Title
CA2489982C (en) Preparation of microbial oil-containing polyunsaturated fatty acids
EP1513941B1 (en) Preparation of microbial oil containing polyunsaturated fatty acids
EP0904339B1 (en) Sterol extraction with a polar solvent to give low sterol microbial oil
CA2501880C (en) Process for producing microbial fat or oil having lowered unsaponifiable matter content and said fat or oil
HK1158697A (en) Preparation of microbial oil containing polyunsaturated fatty acids
HK1152549A (en) Preparation of microbial oil containing polyunsaturated fatty acids
KR20140146156A (ko) 다가불포화 지방산을 함유하는 미생물 오일의 제조방법
HK1146736A (en) Preparation of microbial oil containing polyunsaturated fatty acids

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20230620