CA2485513A1 - Phytases modifiees - Google Patents

Phytases modifiees Download PDF

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Publication number
CA2485513A1
CA2485513A1 CA002485513A CA2485513A CA2485513A1 CA 2485513 A1 CA2485513 A1 CA 2485513A1 CA 002485513 A CA002485513 A CA 002485513A CA 2485513 A CA2485513 A CA 2485513A CA 2485513 A1 CA2485513 A1 CA 2485513A1
Authority
CA
Canada
Prior art keywords
ala
ser
phytase
thr
polypeptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002485513A
Other languages
English (en)
Inventor
Jan Metske Van Der Laan
Stefanus Turk
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF SE
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2485513A1 publication Critical patent/CA2485513A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fodder In General (AREA)

Abstract

La présente invention a trait à des phytases modifiées. Ces phytases sont modifiées en différentes positions par rapport à une phytase type, notamment en vue d'accroître la thermostabilité de la phytase modifiée par rapport à celle de la phytase type. Les phytases modifiées présentent en outre des propriétés favorables préservées de la phytase d'Aspergillus niger, notamment grâce à des résidus d'acides aminés spécifiques de la phytase d'Aspergillus niger préservés dans la phytase modifiées.
CA002485513A 2002-05-30 2003-05-28 Phytases modifiees Abandoned CA2485513A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP02100608 2002-05-30
EP02100608.5 2002-05-30
PCT/EP2003/005729 WO2003102174A2 (fr) 2002-05-30 2003-05-28 Phytases modifiees

Publications (1)

Publication Number Publication Date
CA2485513A1 true CA2485513A1 (fr) 2003-12-11

Family

ID=29595046

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002485513A Abandoned CA2485513A1 (fr) 2002-05-30 2003-05-28 Phytases modifiees

Country Status (16)

Country Link
EP (1) EP1513931A2 (fr)
JP (1) JP2005533492A (fr)
KR (1) KR20050038593A (fr)
CN (1) CN1656217A (fr)
AR (2) AR039939A1 (fr)
AU (1) AU2003238448A1 (fr)
BR (1) BR0311287A (fr)
CA (1) CA2485513A1 (fr)
MX (1) MXPA04011224A (fr)
NO (1) NO20044802L (fr)
PE (1) PE20040537A1 (fr)
PL (1) PL372824A1 (fr)
RU (1) RU2329301C2 (fr)
TW (2) TW200401033A (fr)
WO (1) WO2003102174A2 (fr)
ZA (1) ZA200410369B (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0422052D0 (en) 2004-10-04 2004-11-03 Dansico As Enzymes
ES2531434T3 (es) 2006-04-04 2015-03-16 Novozymes A/S Variantes de fitasa
DE102006053059A1 (de) 2006-11-10 2008-05-15 Ab Enzymes Gmbh Polypeptid mit Phytaseaktivität und erhöhter Temperaturstabilität der Enzymaktivität sowie dieses codierende Nukleotidsequenz
CN102854158B (zh) * 2011-07-01 2016-01-20 北京昕大洋科技发展有限公司 一种快速测定植酸酶耐温性能的方法
CN105441406A (zh) * 2014-08-05 2016-03-30 北京大学 植酸酶变体
CN108251439B (zh) * 2018-01-11 2021-03-30 山西大学 一种人工改造的耐胰蛋白酶的植酸酶及其制备方法和应用
CN117721093A (zh) * 2020-04-29 2024-03-19 南京百斯杰生物工程有限公司 一种亲本植酸酶变体

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60030412T2 (de) * 1999-01-22 2007-08-30 Novozymes A/S Verbesserte phytasen

Also Published As

Publication number Publication date
WO2003102174A2 (fr) 2003-12-11
BR0311287A (pt) 2005-03-29
AR039939A1 (es) 2005-03-09
JP2005533492A (ja) 2005-11-10
RU2329301C2 (ru) 2008-07-20
TW200403341A (en) 2004-03-01
AR039938A1 (es) 2005-03-09
ZA200410369B (en) 2006-06-28
PL372824A1 (en) 2005-08-08
NO20044802L (no) 2004-12-08
WO2003102174A3 (fr) 2004-03-25
EP1513931A2 (fr) 2005-03-16
KR20050038593A (ko) 2005-04-27
TW200401033A (en) 2004-01-16
CN1656217A (zh) 2005-08-17
AU2003238448A1 (en) 2003-12-19
MXPA04011224A (es) 2005-01-25
PE20040537A1 (es) 2004-11-04
RU2004139054A (ru) 2005-06-27

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Legal Events

Date Code Title Description
FZDE Discontinued