CA2477452A1 - Cloning of cytochrome p450 genes from nicotiana - Google Patents

Cloning of cytochrome p450 genes from nicotiana Download PDF

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Publication number
CA2477452A1
CA2477452A1 CA002477452A CA2477452A CA2477452A1 CA 2477452 A1 CA2477452 A1 CA 2477452A1 CA 002477452 A CA002477452 A CA 002477452A CA 2477452 A CA2477452 A CA 2477452A CA 2477452 A1 CA2477452 A1 CA 2477452A1
Authority
CA
Canada
Prior art keywords
nucleic acid
seq
plant
acid molecule
amino acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002477452A
Other languages
English (en)
French (fr)
Inventor
Dongmei Xu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
US Smokeless Tobacco Co LLC
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2477452A1 publication Critical patent/CA2477452A1/en
Abandoned legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0071Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
CA002477452A 2002-03-12 2003-03-12 Cloning of cytochrome p450 genes from nicotiana Abandoned CA2477452A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US36368402P 2002-03-12 2002-03-12
US60/363,684 2002-03-12
PCT/US2003/007430 WO2003078577A2 (en) 2002-03-12 2003-03-12 Cloning of cytochrome p450 genes from nicotiana

Publications (1)

Publication Number Publication Date
CA2477452A1 true CA2477452A1 (en) 2003-09-25

Family

ID=28041798

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002477452A Abandoned CA2477452A1 (en) 2002-03-12 2003-03-12 Cloning of cytochrome p450 genes from nicotiana

Country Status (10)

Country Link
EP (1) EP1572911A4 (enExample)
JP (2) JP2005531297A (enExample)
KR (1) KR20040091124A (enExample)
CN (1) CN101166824A (enExample)
AP (1) AP2004003122A0 (enExample)
AU (1) AU2003220165A1 (enExample)
BR (1) BR0308351A (enExample)
CA (1) CA2477452A1 (enExample)
MX (1) MXPA04008785A (enExample)
WO (1) WO2003078577A2 (enExample)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7700834B2 (en) 2001-11-13 2010-04-20 U.S. Smokless Tobacco Company Nicotiana nucleic acid molecules and uses thereof
US8592663B2 (en) 2001-11-13 2013-11-26 U.S. Smokeless Tobacco Company Llc Tobacco nicotine demethylase genomic clone and uses thereof
US7812227B2 (en) 2001-11-13 2010-10-12 U.S. Smokeless Tobacco Company Cloning of cytochrome p450 genes from nicotiana
US10266836B2 (en) 2001-11-13 2019-04-23 U.S. Smokeless Tobacco Company Llc Tobacco nicotine demethylase genomic clone and uses thereof
US7855318B2 (en) 2001-11-13 2010-12-21 U.S. Smokeless Tobacco Company Cloning of cytochrome P450 genes from Nicotiana
US7700851B2 (en) * 2001-11-13 2010-04-20 U.S. Smokeless Tobacco Company Tobacco nicotine demethylase genomic clone and uses thereof
CN102321641A (zh) * 2003-10-16 2012-01-18 美国无烟烟草有限责任公司 从烟草克隆细胞色素p450基因
JP4519137B2 (ja) * 2003-10-16 2010-08-04 ユーエス スモークレス タバコ カンパニー タバコ属(Nicotiana)からのチトクロムp450遺伝子のクローニング
EP2330191A1 (en) 2003-10-16 2011-06-08 U.S. Smokeless Tobacco Company Cloning of cytochrome P450 genes from nicotiana
AU2011232795B2 (en) * 2004-04-29 2015-05-28 U.S. Smokeless Tobacco Company Llc Nicotiana nucleic acid molecules and uses thereof
JP5106103B2 (ja) * 2004-04-29 2012-12-26 ユーエス スモークレス タバコ カンパニー リミテッド ライアビリティ カンパニー タバコニコチンデメチラーゼゲノムクローンおよびその用途
CN101384710B (zh) * 2004-04-29 2012-05-02 美国无烟烟草有限责任公司 烟草烟碱脱甲基酶基因组克隆及其应用
WO2005111217A2 (en) * 2004-04-29 2005-11-24 U.S. Smokeless Tobacco Company Nicotiana nucleic acid molecules and uses thereof
EP2933328A1 (en) * 2004-04-29 2015-10-21 U.S. Smokeless Tobacco Company LLC Nicotiana nucleic acid molecules and uses thereof
ES2373881T3 (es) * 2004-04-29 2012-02-09 U.S. Smokeless Tobacco Company Llc Clon genómico de nicotina desmetilasa de tabaco y usos del mismo.
US8586837B2 (en) 2004-04-29 2013-11-19 U.S. Smokeless Tobacco Company Llc Nicotiana nucleic acid molecules and uses thereof
JP4753937B2 (ja) * 2004-04-29 2011-08-24 ユーエス スモークレス タバコ カンパニー リミテッド ライアビリティ カンパニー Nicotiana核酸分子およびその用途
EP1851317B1 (en) 2005-02-23 2011-10-26 North Carolina State University Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes
EP2094841B1 (en) * 2006-12-13 2012-03-14 DSM Sinochem Pharmaceuticals Netherlands B.V. Process for preparing pravastatin
US8319011B2 (en) 2006-12-15 2012-11-27 U.S. Smokeless Tobacco Company Llc Tobacco plants having reduced nicotine demethylase activity
US11332753B2 (en) 2006-12-15 2022-05-17 U.S. Smokeless Tobacco Company Llc Tobacco plants having reduced nicotine demethylase activity
BRPI0820042B1 (pt) 2007-11-12 2020-05-19 North Carolina State University método de obtenção de uma planta de tabaco, ou célula ou parte desta, tendo níveis reduzidos de nornicotina, produto de tabaco, método para fabricar um produto de tabaco, polinucleotídeo isolado, cassete de expressão, polipetpídeo isolado e método de obtenção de uma planta, ou parte de planta desta, do gênero nicotiana
PH12012501393B1 (en) 2010-01-15 2017-10-25 Univ North Carolina State Compositions and methods for minimizing nornicotine synthesis in tobacco
KR20140034160A (ko) 2011-02-28 2014-03-19 알트리아 클라이언트 서비시스 인코포레이티드 담배 근친교배 식물체 albex1f 및 albex1ms
BR112013021515A2 (pt) * 2011-02-28 2018-06-12 Univ North Carolina State plantas congênitas de tabaco ncbex1f, ncbex1ms e nc ex90
WO2014110363A1 (en) 2013-01-11 2014-07-17 North Carolina State University Tobacco inbred plants k326 src, cms k326 src, k346 src, cms k346 src, nc1562-1 src, nctg-61 src, cms nctg-61 src and hybrid nc196 src
EP2964016A1 (en) 2013-03-05 2016-01-13 North Carolina State University Tobacco inbred and hybrid plants and uses thereof
CN106659232A (zh) 2014-03-03 2017-05-10 北卡罗莱纳州立大学 烟草近交和杂种植物以及由其制得的烟草产品
EP3113602A1 (en) 2014-03-03 2017-01-11 North Carolina State University Tobacco inbred and hybrid plants and tobacco products made thereof
EP3113603A1 (en) 2014-03-03 2017-01-11 North Carolina State University Tobacco inbred and hybrid plants and tobacco products made thereof
KR102201082B1 (ko) 2018-10-30 2021-01-08 인하대학교 산학협력단 산소환원반응용 니켈-코발트 산화물의 산소결핍의 유도 방법 및 그 방법에 의한 니켈-코발트 산화물

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002244262B2 (en) * 2001-03-09 2007-07-12 University Of Kentucky Research Foundation Cytochrome P450s and uses thereof
EP2336305A3 (en) * 2002-10-16 2011-10-05 U.S. Smokeless Tobacco Company LLC Cloning of cytochrome P450 genes from Nicotiana

Also Published As

Publication number Publication date
AU2003220165A8 (en) 2008-03-06
AU2003220165A1 (en) 2003-09-29
KR20040091124A (ko) 2004-10-27
WO2003078577A9 (en) 2004-01-22
MXPA04008785A (es) 2005-09-30
JP2005531297A (ja) 2005-10-20
EP1572911A2 (en) 2005-09-14
BR0308351A (pt) 2007-04-17
AP2004003122A0 (en) 2004-09-30
WO2003078577A8 (en) 2008-01-10
EP1572911A4 (en) 2008-06-25
CN101166824A (zh) 2008-04-23
WO2003078577A2 (en) 2003-09-25
JP2010131027A (ja) 2010-06-17

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued