CN101166824A - 克隆来自烟草的细胞色素p450基因 - Google Patents

克隆来自烟草的细胞色素p450基因 Download PDF

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Publication number
CN101166824A
CN101166824A CNA038057476A CN03805747A CN101166824A CN 101166824 A CN101166824 A CN 101166824A CN A038057476 A CNA038057476 A CN A038057476A CN 03805747 A CN03805747 A CN 03805747A CN 101166824 A CN101166824 A CN 101166824A
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China
Prior art keywords
nucleic acid
seq
plants
plant
acid molecule
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CNA038057476A
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English (en)
Chinese (zh)
Inventor
D·徐
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US Smokeless Tobacco Co LLC
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US Smokeless Tobacco Co LLC
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Publication of CN101166824A publication Critical patent/CN101166824A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
CNA038057476A 2002-03-12 2003-03-12 克隆来自烟草的细胞色素p450基因 Pending CN101166824A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US36368402P 2002-03-12 2002-03-12
US60/363,684 2002-03-12

Publications (1)

Publication Number Publication Date
CN101166824A true CN101166824A (zh) 2008-04-23

Family

ID=28041798

Family Applications (1)

Application Number Title Priority Date Filing Date
CNA038057476A Pending CN101166824A (zh) 2002-03-12 2003-03-12 克隆来自烟草的细胞色素p450基因

Country Status (10)

Country Link
EP (1) EP1572911A4 (enExample)
JP (2) JP2005531297A (enExample)
KR (1) KR20040091124A (enExample)
CN (1) CN101166824A (enExample)
AP (1) AP2004003122A0 (enExample)
AU (1) AU2003220165A1 (enExample)
BR (1) BR0308351A (enExample)
CA (1) CA2477452A1 (enExample)
MX (1) MXPA04008785A (enExample)
WO (1) WO2003078577A2 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103687480A (zh) * 2011-02-28 2014-03-26 北卡罗莱纳州立大学 烟草近交植物ncbex1f、ncbex1ms和nc ex90

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10266836B2 (en) 2001-11-13 2019-04-23 U.S. Smokeless Tobacco Company Llc Tobacco nicotine demethylase genomic clone and uses thereof
US7812227B2 (en) 2001-11-13 2010-10-12 U.S. Smokeless Tobacco Company Cloning of cytochrome p450 genes from nicotiana
US7700834B2 (en) 2001-11-13 2010-04-20 U.S. Smokless Tobacco Company Nicotiana nucleic acid molecules and uses thereof
US8592663B2 (en) 2001-11-13 2013-11-26 U.S. Smokeless Tobacco Company Llc Tobacco nicotine demethylase genomic clone and uses thereof
US7855318B2 (en) 2001-11-13 2010-12-21 U.S. Smokeless Tobacco Company Cloning of cytochrome P450 genes from Nicotiana
US7700851B2 (en) * 2001-11-13 2010-04-20 U.S. Smokeless Tobacco Company Tobacco nicotine demethylase genomic clone and uses thereof
AU2004282576B2 (en) * 2002-10-16 2010-01-28 U S Smokeless Tobacco Company Llc Cloning of cytochrome P450 genes from nicotiana
CN102703477A (zh) * 2003-10-16 2012-10-03 美国无烟烟草有限责任公司 从烟草克隆细胞色素p450基因
ZA200602938B (en) * 2003-10-16 2008-06-25 Us Smokeless Tobacco Company Cloning of cytochrome p450 genes from Nicotiana
AU2005243214B2 (en) * 2004-04-29 2011-07-07 U.S. Smokeless Tobacco Company Llc Nicotiana nucleic acid molecules and uses thereof
CN101979583B (zh) * 2004-04-29 2016-08-10 美国无烟烟草有限责任公司 烟草烟碱脱甲基酶基因组克隆及其应用
US8586837B2 (en) 2004-04-29 2013-11-19 U.S. Smokeless Tobacco Company Llc Nicotiana nucleic acid molecules and uses thereof
EP2933328A1 (en) * 2004-04-29 2015-10-21 U.S. Smokeless Tobacco Company LLC Nicotiana nucleic acid molecules and uses thereof
JP5106103B2 (ja) * 2004-04-29 2012-12-26 ユーエス スモークレス タバコ カンパニー リミテッド ライアビリティ カンパニー タバコニコチンデメチラーゼゲノムクローンおよびその用途
JP4753937B2 (ja) * 2004-04-29 2011-08-24 ユーエス スモークレス タバコ カンパニー リミテッド ライアビリティ カンパニー Ｎｉｃｏｔｉａｎａ核酸分子およびその用途
AU2011232795B2 (en) * 2004-04-29 2015-05-28 U.S. Smokeless Tobacco Company Llc Nicotiana nucleic acid molecules and uses thereof
CN101384710B (zh) * 2004-04-29 2012-05-02 美国无烟烟草有限责任公司 烟草烟碱脱甲基酶基因组克隆及其应用
EP1851317B1 (en) 2005-02-23 2011-10-26 North Carolina State University Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes
DK2094841T3 (da) * 2006-12-13 2012-05-29 Dsm Sinochem Pharm Nl Bv Fremgangsmåde til fremstilling af pravastatin
US8319011B2 (en) 2006-12-15 2012-11-27 U.S. Smokeless Tobacco Company Llc Tobacco plants having reduced nicotine demethylase activity
US11332753B2 (en) 2006-12-15 2022-05-17 U.S. Smokeless Tobacco Company Llc Tobacco plants having reduced nicotine demethylase activity
US8124851B2 (en) 2007-11-12 2012-02-28 North Carolina State University Alteration of tobacco alkaloid content through modification of specific cytochrome P450 genes
MX337123B (es) 2010-01-15 2016-02-12 Univ North Carolina State Composiciones y metodos para minimizar la sintesis de nornicotina en el tabaco.
CN103562393A (zh) 2011-02-28 2014-02-05 奥驰亚客户服务公司 烟草近交植物albex1f和albex1ms
US9603335B2 (en) 2013-01-11 2017-03-28 North Carolina State University Tobacco inbred plants K326 SRC, CMS K326 SRC, K346 SRC, CMS K346 SRC, NC1562-1 SRC, NCTG-61 SRC, CMS NCTG-61 SRC and hybrid NC196 SRC
EP2964016A1 (en) 2013-03-05 2016-01-13 North Carolina State University Tobacco inbred and hybrid plants and uses thereof
EP3113602A1 (en) 2014-03-03 2017-01-11 North Carolina State University Tobacco inbred and hybrid plants and tobacco products made thereof
CN106659232A (zh) 2014-03-03 2017-05-10 北卡罗莱纳州立大学 烟草近交和杂种植物以及由其制得的烟草产品
WO2015134438A1 (en) 2014-03-03 2015-09-11 North Carolina State University Tobacco inbred and hybrid plants and tobacco products made thereof
KR102201082B1 (ko) 2018-10-30 2021-01-08 인하대학교 산학협력단 산소환원반응용 니켈-코발트 산화물의 산소결핍의 유도 방법 및 그 방법에 의한 니켈-코발트 산화물

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE513046T1 (de) * 2001-03-09 2011-07-15 Univ Kentucky Res Found Cytochrom p450s und verwendungen davon
JP2006519590A (ja) * 2002-10-16 2006-08-31 ユーエス スモークレス タバコ カンパニー タバコ属由来のシトクロムｐ４５０遺伝子のクローニング

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103687480A (zh) * 2011-02-28 2014-03-26 北卡罗莱纳州立大学 烟草近交植物ncbex1f、ncbex1ms和nc ex90

Also Published As

Publication number Publication date
BR0308351A (pt) 2007-04-17
EP1572911A2 (en) 2005-09-14
MXPA04008785A (es) 2005-09-30
WO2003078577A2 (en) 2003-09-25
AU2003220165A8 (en) 2008-03-06
EP1572911A4 (en) 2008-06-25
JP2005531297A (ja) 2005-10-20
AU2003220165A1 (en) 2003-09-29
KR20040091124A (ko) 2004-10-27
JP2010131027A (ja) 2010-06-17
WO2003078577A9 (en) 2004-01-22
CA2477452A1 (en) 2003-09-25
WO2003078577A8 (en) 2008-01-10
AP2004003122A0 (en) 2004-09-30

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Open date: 20080423