CA2435753A1 - Renaturation and activation of the proteinase k zmyogen produced in inclusion bodies - Google Patents

Renaturation and activation of the proteinase k zmyogen produced in inclusion bodies Download PDF

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Publication number
CA2435753A1
CA2435753A1 CA002435753A CA2435753A CA2435753A1 CA 2435753 A1 CA2435753 A1 CA 2435753A1 CA 002435753 A CA002435753 A CA 002435753A CA 2435753 A CA2435753 A CA 2435753A CA 2435753 A1 CA2435753 A1 CA 2435753A1
Authority
CA
Canada
Prior art keywords
proteinase
zymogenic
recombinant
folding
concentration
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002435753A
Other languages
English (en)
French (fr)
Inventor
Rainer Mueller
Johann-Peter Thalhofer
Bernhard Rexer
Rainer Schmuck
Frank Geipel
Stephan Glaser
Helmut Schoen
Thomas Meier
Rainer Rudolph
Hauke Lilie
Bjoern Schott
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2435753A1 publication Critical patent/CA2435753A1/en
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21064—Peptidase K (3.4.21.64)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA002435753A 2001-02-09 2002-02-08 Renaturation and activation of the proteinase k zmyogen produced in inclusion bodies Abandoned CA2435753A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10105912A DE10105912A1 (de) 2001-02-09 2001-02-09 Rekombinante Proteinase K
DE10105912.4 2001-02-09
PCT/EP2002/001322 WO2002072634A2 (de) 2001-02-09 2002-02-08 Rekombinante proteinase k

Publications (1)

Publication Number Publication Date
CA2435753A1 true CA2435753A1 (en) 2002-09-19

Family

ID=7673417

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002435753A Abandoned CA2435753A1 (en) 2001-02-09 2002-02-08 Renaturation and activation of the proteinase k zmyogen produced in inclusion bodies

Country Status (8)

Country Link
US (1) US20070099283A1 (https=)
EP (1) EP1360284B1 (https=)
JP (1) JP2004525631A (https=)
AT (1) ATE401393T1 (https=)
CA (1) CA2435753A1 (https=)
CZ (1) CZ20032165A3 (https=)
DE (2) DE10105912A1 (https=)
WO (1) WO2002072634A2 (https=)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7153666B2 (en) 2003-07-17 2006-12-26 General Atomics Methods and compositions for determination of glycated proteins
ES2404059T3 (es) * 2006-07-25 2013-05-23 General Atomics Procedimientos para analizar el porcentaje de hemoglobina glicada
US7943385B2 (en) 2006-07-25 2011-05-17 General Atomics Methods for assaying percentage of glycated hemoglobin
WO2008063378A2 (en) 2006-11-01 2008-05-29 Ventana Medical Systems, Inc. Haptens, hapten conjugates, compositions thereof and method for their preparation and use
US8148105B2 (en) * 2007-03-16 2012-04-03 The Board Of Trustees Of The Leland Stanford Junior University Scaleable manufacturing process for cysteine endoprotease B, isoform 2
US7682789B2 (en) * 2007-05-04 2010-03-23 Ventana Medical Systems, Inc. Method for quantifying biomolecules conjugated to a nanoparticle
JP2010528285A (ja) 2007-05-23 2010-08-19 ベンタナ・メデイカル・システムズ・インコーポレーテツド 免疫組織化学およびｉｎｓｉｔｕハイブリダーゼーションのためのポリマー担体
EP2283149A1 (en) * 2008-05-13 2011-02-16 General Atomics Electrochemical biosensor for direct determination of percentage of glycated hemoglobin
WO2009149013A2 (en) 2008-06-05 2009-12-10 Ventana Medical Systems, Inc. Compositions comprising nanomaterials and method for using such compositions for histochemical processes
USPP22463P3 (en) * 2010-02-16 2012-01-17 Menachem Bornstein Gypsophila plant named ‘Pearl Blossom’
EP2423217A1 (en) 2010-08-23 2012-02-29 Forschungsverbund Berlin E.V. Peptide and protein affinity tag from mistic protein
CN112592931A (zh) * 2020-12-31 2021-04-02 安徽丰原发酵技术工程研究有限公司 一种生产重组蛋白酶k的方法
CN118165964B (zh) * 2024-04-11 2025-01-24 铭诚惠众(江苏)药物研究有限公司 一种重组蛋白酶k的纯化方法及其应用

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5332805A (en) * 1984-02-03 1994-07-26 Celltech Limited Process for the recovery of recombinantly produced chymosin from insoluble aggregate
US5082775A (en) * 1984-05-11 1992-01-21 Berlex Laboratories, Inc. Efficient process for isolating insoluble heterologous protein using non-ionic detergents
DE3537708A1 (de) * 1985-10-23 1987-04-23 Boehringer Mannheim Gmbh Verfahren zur aktivierung von t-pa nach expression in prokaryonten
US5453363A (en) * 1985-10-23 1995-09-26 Boehringer Mannheim Gmbh Process for the activation of t-PA or Ing after genetic expression in prokaryotes
AU617996B2 (en) * 1987-04-03 1991-12-12 Amgen, Inc. Novel proteolytic enzymes
DE3835350A1 (de) * 1988-10-17 1990-04-19 Boehringer Mannheim Gmbh Aktivierung von gentechnologisch hergestellten, in prokaryonten exprimierten antikoerpern
DE3903581A1 (de) * 1989-02-07 1990-08-16 Boehringer Mannheim Gmbh Gewebs-plasminogenaktivator-derivat
US5191063A (en) * 1989-05-02 1993-03-02 University Of Medicine And Dentistry Of New Jersey Production of biologically active polypeptides by treatment with an exogenous peptide sequence
US5606031A (en) * 1990-04-06 1997-02-25 Lile; Jack Production and purification of biologically active recombinant neurotrophic protein in bacteria
US5144006A (en) * 1991-06-13 1992-09-01 The Rockefeller University Oxidative folding of peptide and protein substrates using hydrocarbon sulfoxides
US5792623A (en) * 1992-04-06 1998-08-11 Immuno Aktiengesellschaft Method for producing activated blood factors with a protease and a detergent
DE4225427A1 (de) * 1992-07-31 1994-02-03 Boehringer Mannheim Gmbh Verfahren zur Gewinnung rekombinanter, biologisch aktiver, eukaryontischer alkalischer Phosphatase
CA2155335C (en) * 1993-02-04 2001-06-05 HANS CHRISTIAN THõGERSEN Improved method for the refolding of proteins
US5747654A (en) * 1993-06-14 1998-05-05 The United States Of America As Represented By The Department Of Health And Human Services Recombinant disulfide-stabilized polypeptide fragments having binding specificity
JPH0748398A (ja) * 1993-08-03 1995-02-21 Nippon Oil Co Ltd 変性蛋白質の再生方法及び変性蛋白質再生剤
US5550262A (en) * 1994-11-14 1996-08-27 Cephalon, Inc. Multicatalytic protease inhibitors
IL117350A0 (en) * 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
US6342585B1 (en) * 1996-06-11 2002-01-29 Roche Diagnostics Gmbh Method of activating denatured protein
US6426075B1 (en) * 1996-11-06 2002-07-30 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Protease-activatable pseudomonas exotoxin A-like proproteins
US20030207402A1 (en) * 1997-08-22 2003-11-06 Erhard Kopetzki Autocatalytically activatable zymogenic precursors of proteases and their use
WO2000034504A2 (en) * 1998-12-10 2000-06-15 Genetic Vectors, Inc. Method of protein removal
US7351549B2 (en) * 2000-01-24 2008-04-01 Polymun Scientific Immunbiologische Forschung Gmbh Method for the manufacture of recombinant trypsin
US7001724B1 (en) * 2000-11-28 2006-02-21 Applera Corporation Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases
MXPA03004835A (es) * 2000-12-15 2005-04-19 Boehringer Ingelheim Ltd Proteasa ns2/3 activa, purificada de virus de hepatitis c.
US7214484B2 (en) * 2002-12-17 2007-05-08 Sigma-Aldrich Co. Compositions and methods for nucleic acid extraction from biological samples

Also Published As

Publication number Publication date
DE10105912A1 (de) 2002-08-14
EP1360284B1 (de) 2008-07-16
DE50212510D1 (de) 2008-08-28
US20070099283A1 (en) 2007-05-03
WO2002072634A2 (de) 2002-09-19
CZ20032165A3 (cs) 2004-10-13
EP1360284A2 (de) 2003-11-12
JP2004525631A (ja) 2004-08-26
WO2002072634A3 (de) 2003-03-13
ATE401393T1 (de) 2008-08-15

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