JP2004525631A - 組換えプロテイナーゼｋ - Google Patents

組換えプロテイナーゼｋ Download PDF

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Publication number
JP2004525631A
JP2004525631A JP2002571547A JP2002571547A JP2004525631A JP 2004525631 A JP2004525631 A JP 2004525631A JP 2002571547 A JP2002571547 A JP 2002571547A JP 2002571547 A JP2002571547 A JP 2002571547A JP 2004525631 A JP2004525631 A JP 2004525631A
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JP
Japan
Prior art keywords
proteinase
folding
concentration
buffer
recombinant
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Application number
JP2002571547A
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English (en)
Japanese (ja)
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JP2004525631A5 (https=
Inventor
ミュラー，ライナー
タールホファー，ヨハン−ペーター
レクサー，ベルンハルト
シュムック，ライナー
ガイペル，フランク
グラーゼル，シュテファン
シェーン，ヘルムート
マイアー，トーマス
ルドルフ，ライナー
リーリー，ハウケ
ショット，ビェルン
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
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Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of JP2004525631A publication Critical patent/JP2004525631A/ja
Publication of JP2004525631A5 publication Critical patent/JP2004525631A5/ja
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21064—Peptidase K (3.4.21.64)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2002571547A 2001-02-09 2002-02-08 組換えプロテイナーゼｋ Pending JP2004525631A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10105912A DE10105912A1 (de) 2001-02-09 2001-02-09 Rekombinante Proteinase K
PCT/EP2002/001322 WO2002072634A2 (de) 2001-02-09 2002-02-08 Rekombinante proteinase k

Publications (2)

Publication Number Publication Date
JP2004525631A true JP2004525631A (ja) 2004-08-26
JP2004525631A5 JP2004525631A5 (https=) 2006-12-07

Family

ID=7673417

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2002571547A Pending JP2004525631A (ja) 2001-02-09 2002-02-08 組換えプロテイナーゼｋ

Country Status (8)

Country Link
US (1) US20070099283A1 (https=)
EP (1) EP1360284B1 (https=)
JP (1) JP2004525631A (https=)
AT (1) ATE401393T1 (https=)
CA (1) CA2435753A1 (https=)
CZ (1) CZ20032165A3 (https=)
DE (2) DE10105912A1 (https=)
WO (1) WO2002072634A2 (https=)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7153666B2 (en) 2003-07-17 2006-12-26 General Atomics Methods and compositions for determination of glycated proteins
ES2404059T3 (es) * 2006-07-25 2013-05-23 General Atomics Procedimientos para analizar el porcentaje de hemoglobina glicada
US7943385B2 (en) 2006-07-25 2011-05-17 General Atomics Methods for assaying percentage of glycated hemoglobin
WO2008063378A2 (en) 2006-11-01 2008-05-29 Ventana Medical Systems, Inc. Haptens, hapten conjugates, compositions thereof and method for their preparation and use
US8148105B2 (en) * 2007-03-16 2012-04-03 The Board Of Trustees Of The Leland Stanford Junior University Scaleable manufacturing process for cysteine endoprotease B, isoform 2
US7682789B2 (en) * 2007-05-04 2010-03-23 Ventana Medical Systems, Inc. Method for quantifying biomolecules conjugated to a nanoparticle
JP2010528285A (ja) 2007-05-23 2010-08-19 ベンタナ・メデイカル・システムズ・インコーポレーテツド 免疫組織化学およびｉｎｓｉｔｕハイブリダーゼーションのためのポリマー担体
EP2283149A1 (en) * 2008-05-13 2011-02-16 General Atomics Electrochemical biosensor for direct determination of percentage of glycated hemoglobin
WO2009149013A2 (en) 2008-06-05 2009-12-10 Ventana Medical Systems, Inc. Compositions comprising nanomaterials and method for using such compositions for histochemical processes
USPP22463P3 (en) * 2010-02-16 2012-01-17 Menachem Bornstein Gypsophila plant named ‘Pearl Blossom’
EP2423217A1 (en) 2010-08-23 2012-02-29 Forschungsverbund Berlin E.V. Peptide and protein affinity tag from mistic protein
CN112592931A (zh) * 2020-12-31 2021-04-02 安徽丰原发酵技术工程研究有限公司 一种生产重组蛋白酶k的方法
CN118165964B (zh) * 2024-04-11 2025-01-24 铭诚惠众(江苏)药物研究有限公司 一种重组蛋白酶k的纯化方法及其应用

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5332805A (en) * 1984-02-03 1994-07-26 Celltech Limited Process for the recovery of recombinantly produced chymosin from insoluble aggregate
US5082775A (en) * 1984-05-11 1992-01-21 Berlex Laboratories, Inc. Efficient process for isolating insoluble heterologous protein using non-ionic detergents
DE3537708A1 (de) * 1985-10-23 1987-04-23 Boehringer Mannheim Gmbh Verfahren zur aktivierung von t-pa nach expression in prokaryonten
US5453363A (en) * 1985-10-23 1995-09-26 Boehringer Mannheim Gmbh Process for the activation of t-PA or Ing after genetic expression in prokaryotes
AU617996B2 (en) * 1987-04-03 1991-12-12 Amgen, Inc. Novel proteolytic enzymes
DE3835350A1 (de) * 1988-10-17 1990-04-19 Boehringer Mannheim Gmbh Aktivierung von gentechnologisch hergestellten, in prokaryonten exprimierten antikoerpern
DE3903581A1 (de) * 1989-02-07 1990-08-16 Boehringer Mannheim Gmbh Gewebs-plasminogenaktivator-derivat
US5191063A (en) * 1989-05-02 1993-03-02 University Of Medicine And Dentistry Of New Jersey Production of biologically active polypeptides by treatment with an exogenous peptide sequence
US5606031A (en) * 1990-04-06 1997-02-25 Lile; Jack Production and purification of biologically active recombinant neurotrophic protein in bacteria
US5144006A (en) * 1991-06-13 1992-09-01 The Rockefeller University Oxidative folding of peptide and protein substrates using hydrocarbon sulfoxides
US5792623A (en) * 1992-04-06 1998-08-11 Immuno Aktiengesellschaft Method for producing activated blood factors with a protease and a detergent
DE4225427A1 (de) * 1992-07-31 1994-02-03 Boehringer Mannheim Gmbh Verfahren zur Gewinnung rekombinanter, biologisch aktiver, eukaryontischer alkalischer Phosphatase
CA2155335C (en) * 1993-02-04 2001-06-05 HANS CHRISTIAN THõGERSEN Improved method for the refolding of proteins
US5747654A (en) * 1993-06-14 1998-05-05 The United States Of America As Represented By The Department Of Health And Human Services Recombinant disulfide-stabilized polypeptide fragments having binding specificity
JPH0748398A (ja) * 1993-08-03 1995-02-21 Nippon Oil Co Ltd 変性蛋白質の再生方法及び変性蛋白質再生剤
US5550262A (en) * 1994-11-14 1996-08-27 Cephalon, Inc. Multicatalytic protease inhibitors
IL117350A0 (en) * 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
US6342585B1 (en) * 1996-06-11 2002-01-29 Roche Diagnostics Gmbh Method of activating denatured protein
US6426075B1 (en) * 1996-11-06 2002-07-30 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Protease-activatable pseudomonas exotoxin A-like proproteins
US20030207402A1 (en) * 1997-08-22 2003-11-06 Erhard Kopetzki Autocatalytically activatable zymogenic precursors of proteases and their use
WO2000034504A2 (en) * 1998-12-10 2000-06-15 Genetic Vectors, Inc. Method of protein removal
US7351549B2 (en) * 2000-01-24 2008-04-01 Polymun Scientific Immunbiologische Forschung Gmbh Method for the manufacture of recombinant trypsin
US7001724B1 (en) * 2000-11-28 2006-02-21 Applera Corporation Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases
MXPA03004835A (es) * 2000-12-15 2005-04-19 Boehringer Ingelheim Ltd Proteasa ns2/3 activa, purificada de virus de hepatitis c.
US7214484B2 (en) * 2002-12-17 2007-05-08 Sigma-Aldrich Co. Compositions and methods for nucleic acid extraction from biological samples

Also Published As

Publication number Publication date
DE10105912A1 (de) 2002-08-14
EP1360284B1 (de) 2008-07-16
CA2435753A1 (en) 2002-09-19
DE50212510D1 (de) 2008-08-28
US20070099283A1 (en) 2007-05-03
WO2002072634A2 (de) 2002-09-19
CZ20032165A3 (cs) 2004-10-13
EP1360284A2 (de) 2003-11-12
WO2002072634A3 (de) 2003-03-13
ATE401393T1 (de) 2008-08-15

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