CA2378016A1 - Nouveaux moyens de transformation de champignons et leur utilisation pour la production d'une proteine heterologue - Google Patents

Nouveaux moyens de transformation de champignons et leur utilisation pour la production d'une proteine heterologue Download PDF

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Publication number
CA2378016A1
CA2378016A1 CA002378016A CA2378016A CA2378016A1 CA 2378016 A1 CA2378016 A1 CA 2378016A1 CA 002378016 A CA002378016 A CA 002378016A CA 2378016 A CA2378016 A CA 2378016A CA 2378016 A1 CA2378016 A1 CA 2378016A1
Authority
CA
Canada
Prior art keywords
sojae
aspergillus sojae
gene
aspergillus
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002378016A
Other languages
English (en)
Inventor
Margreet Heerikhuisen
Cornelis Van Den Hondel
Peter Punt
Nick Van Biezen
Alwin Albers
Kurt Vogel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
F Hoffmann La Roche AG
Nederlandse Organisatie voor Toegepast Natuurwetenschappelijk Onderzoek TNO
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG, Nederlandse Organisatie voor Toegepast Natuurwetenschappelijk Onderzoek TNO filed Critical F Hoffmann La Roche AG
Publication of CA2378016A1 publication Critical patent/CA2378016A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention concerne un Aspergillus sojae) recombinant comprenant un gène S (amdS) acétamidase introduit en tant que marqueur sélectable. Elle concerne également un Aspergillus sojae qui exprime la croissance dans un milieu comprenant uracile et un acide fluoro-orotique, mais n'exprime pas la croissance dans un milieu comprenant uridine et un acide fluoro-orotique (c'est-à-dire, Aspergillus sojae exprime l'auxotrophie pour l'uracile, est incapable d'utiliser l'uridine, est pyrG négatif, est résistant à l'acide fluoro-orotique, peut être libéré après complémentation avec un gène pyrG) introduit). The Aspergillus sojae comprend en outre une séquence d'acide nucléique codant une phytase ou une protéine ayant une activité phytase ou toute autre protéine ou polypetide hétérologue, et peut être utilisé pour la production biotechnologique de cette phytase ou de ces autres protéines ou polypetides hétérologues. L'invention concerne en outre des mutants supplémentaires présentant une morphologie modifiée. Elle concerne enfin des méthodes de production de tels hôtes d'expression.
CA002378016A 1999-07-30 2000-07-28 Nouveaux moyens de transformation de champignons et leur utilisation pour la production d'une proteine heterologue Abandoned CA2378016A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP99202517 1999-07-30
EP99202516.3 1999-07-30
EP99202516 1999-07-30
EP99202517.1 1999-07-30
PCT/NL2000/000544 WO2001009352A2 (fr) 1999-07-30 2000-07-28 Nouveaux moyens de transformation de champignons et leur utilisation pour la production d'une proteine heterologue

Publications (1)

Publication Number Publication Date
CA2378016A1 true CA2378016A1 (fr) 2001-02-08

Family

ID=26153350

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002378016A Abandoned CA2378016A1 (fr) 1999-07-30 2000-07-28 Nouveaux moyens de transformation de champignons et leur utilisation pour la production d'une proteine heterologue

Country Status (10)

Country Link
EP (1) EP1204758A2 (fr)
JP (1) JP2003518921A (fr)
KR (1) KR20020059350A (fr)
CN (1) CN1369016A (fr)
AR (1) AR024989A1 (fr)
AU (1) AU782116B2 (fr)
BR (1) BR0012856A (fr)
CA (1) CA2378016A1 (fr)
MX (1) MXPA02001061A (fr)
WO (1) WO2001009352A2 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7883872B2 (en) 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
KR20020026456A (ko) 2000-04-13 2002-04-10 에말파브 마크 아론 사상균에서 발현된 ｄｎａ 라이브러리의 고산출량 스크리닝
CA2736661A1 (fr) 2007-09-07 2009-03-12 Dyadic International, Inc. Enzymes fongiques inedites
JP2011239681A (ja) * 2008-09-19 2011-12-01 Kikkoman Corp 麹菌アルカリプロテアーゼプロモーター
CN101503660B (zh) * 2009-02-10 2011-07-27 汪兵 表达嗜热糖化酶的工程菌及其应用
DK3000870T3 (en) * 2011-04-22 2018-04-30 Danisco Us Inc FILAMENTOUS FUNGI WITH CHANGED VISCOSITY PHENOTYPE
EP2699667B1 (fr) * 2011-04-22 2016-04-13 Danisco US Inc. Champignons filamenteux ayant un phénotype de viscosité modifiée

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2748347B2 (ja) * 1989-03-09 1998-05-06 キッコーマン 株式会社 アスペルギルス・ソーヤ形質転換体
DE19526485A1 (de) * 1995-07-20 1997-01-23 Roehm Gmbh Rekombinant hergestellte Leucinaminopeptidase aus Aspergillus sojae
DK0758020T3 (da) * 1995-08-03 2009-04-06 Dsm Ip Assets Bv Amds-gen fra Aspergillus niger kodende for en acetamidase
JP3727780B2 (ja) * 1998-06-12 2005-12-14 キッコーマン株式会社 ロイシンアミノペプチダーゼ遺伝子、組み換え体ｄｎａ及びロイシンアミノペプチダーゼの製造法

Also Published As

Publication number Publication date
AU6323800A (en) 2001-02-19
CN1369016A (zh) 2002-09-11
BR0012856A (pt) 2002-06-11
JP2003518921A (ja) 2003-06-17
KR20020059350A (ko) 2002-07-12
AU782116B2 (en) 2005-07-07
WO2001009352A3 (fr) 2001-11-08
EP1204758A2 (fr) 2002-05-15
AR024989A1 (es) 2002-11-06
WO2001009352A2 (fr) 2001-02-08
MXPA02001061A (es) 2003-04-10

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Legal Events

Date Code Title Description
FZDE Discontinued
FZDE Discontinued

Effective date: 20030730