CA2360543C - Binary probe and clamp composition and methods for target hybridization detection - Google Patents

Binary probe and clamp composition and methods for target hybridization detection Download PDF

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Publication number
CA2360543C
CA2360543C CA002360543A CA2360543A CA2360543C CA 2360543 C CA2360543 C CA 2360543C CA 002360543 A CA002360543 A CA 002360543A CA 2360543 A CA2360543 A CA 2360543A CA 2360543 C CA2360543 C CA 2360543C
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CA
Canada
Prior art keywords
clamp
probe
specific portion
sequence
target
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA002360543A
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English (en)
French (fr)
Other versions
CA2360543A1 (en
Inventor
Kenneth J. Livak
Michael Egholm
Michael W. Hunkapiller
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Applied Biosystems LLC
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Applera Corp
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Publication of CA2360543A1 publication Critical patent/CA2360543A1/en
Application granted granted Critical
Publication of CA2360543C publication Critical patent/CA2360543C/en
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Expired - Fee Related legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6818Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Electrophonic Musical Instruments (AREA)
CA002360543A 1999-01-15 2000-01-14 Binary probe and clamp composition and methods for target hybridization detection Expired - Fee Related CA2360543C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/232,000 US6432642B1 (en) 1999-01-15 1999-01-15 Binary probe and clamp composition and methods for a target hybridization detection
US09/232,000 1999-01-15
PCT/US2000/000972 WO2000041549A2 (en) 1999-01-15 2000-01-14 Binary probe and clamp composition and methods for target hybridization detection

Publications (2)

Publication Number Publication Date
CA2360543A1 CA2360543A1 (en) 2000-07-20
CA2360543C true CA2360543C (en) 2009-03-31

Family

ID=22871476

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002360543A Expired - Fee Related CA2360543C (en) 1999-01-15 2000-01-14 Binary probe and clamp composition and methods for target hybridization detection

Country Status (8)

Country Link
US (4) US6432642B1 (enExample)
EP (1) EP1141387B1 (enExample)
JP (3) JP4644370B2 (enExample)
AT (1) ATE329056T1 (enExample)
AU (1) AU771650B2 (enExample)
CA (1) CA2360543C (enExample)
DE (1) DE60028534T2 (enExample)
WO (1) WO2000041549A2 (enExample)

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CN110283888B (zh) 2013-08-19 2021-06-22 卓异生物公司 用于单分子检测的测定及其应用
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WO2016134191A1 (en) 2015-02-18 2016-08-25 Singular Bio, Inc. Assays for single molecule detection and use thereof
US11788123B2 (en) 2017-05-26 2023-10-17 President And Fellows Of Harvard College Systems and methods for high-throughput image-based screening
KR101899371B1 (ko) 2017-07-25 2018-10-29 (주)엔바이오텍 핵산 복합체 페어, 핵산 복합체 페어를 포함하는 pcr용 키트, 및 핵산 복합체 페어를 이용한 타겟 검출 방법
KR102262260B1 (ko) * 2018-02-08 2021-06-09 주식회사 시선테라퓨틱스 엔도좀 탈출능을 갖는 펩티드 핵산 복합체 및 이의 용도
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Also Published As

Publication number Publication date
ATE329056T1 (de) 2006-06-15
JP4644370B2 (ja) 2011-03-02
EP1141387B1 (en) 2006-06-07
AU771650B2 (en) 2004-04-01
CA2360543A1 (en) 2000-07-20
JP2009017882A (ja) 2009-01-29
WO2000041549A2 (en) 2000-07-20
EP1141387A2 (en) 2001-10-10
DE60028534D1 (de) 2006-07-20
US6432642B1 (en) 2002-08-13
US20070026429A1 (en) 2007-02-01
US20060035217A1 (en) 2006-02-16
US7057025B2 (en) 2006-06-06
WO2000041549A3 (en) 2000-11-30
US8067165B2 (en) 2011-11-29
DE60028534T2 (de) 2007-06-06
AU2850400A (en) 2000-08-01
JP2005160489A (ja) 2005-06-23
JP2002534100A (ja) 2002-10-15
US20100291557A1 (en) 2010-11-18

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