CA2359629C - Schizochytrium pks genes - Google Patents

Schizochytrium pks genes Download PDF

Info

Publication number
CA2359629C
CA2359629C CA2359629A CA2359629A CA2359629C CA 2359629 C CA2359629 C CA 2359629C CA 2359629 A CA2359629 A CA 2359629A CA 2359629 A CA2359629 A CA 2359629A CA 2359629 C CA2359629 C CA 2359629C
Authority
CA
Canada
Prior art keywords
ala
leu
ser
gly
val
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2359629A
Other languages
English (en)
French (fr)
Other versions
CA2359629A1 (en
Inventor
Daniel Facciotti
James George Metz
Michael Lassner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
DSM IP Assets BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DSM IP Assets BV filed Critical DSM IP Assets BV
Publication of CA2359629A1 publication Critical patent/CA2359629A1/en
Application granted granted Critical
Publication of CA2359629C publication Critical patent/CA2359629C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/02—Nutrients, e.g. vitamins, minerals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/21—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pseudomonadaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/28—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Vibrionaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/405—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6434—Docosahexenoic acids [DHA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Diabetes (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Public Health (AREA)
  • Obesity (AREA)
  • Hematology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Endocrinology (AREA)
  • Emergency Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CA2359629A 1999-01-14 2000-01-14 Schizochytrium pks genes Expired - Lifetime CA2359629C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/231,899 1999-01-14
US09/231,899 US6566583B1 (en) 1997-06-04 1999-01-14 Schizochytrium PKS genes
PCT/US2000/000956 WO2000042195A2 (en) 1999-01-14 2000-01-14 Schizochytrium pks genes

Publications (2)

Publication Number Publication Date
CA2359629A1 CA2359629A1 (en) 2000-07-20
CA2359629C true CA2359629C (en) 2015-02-24

Family

ID=22871074

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2359629A Expired - Lifetime CA2359629C (en) 1999-01-14 2000-01-14 Schizochytrium pks genes

Country Status (10)

Country Link
US (6) US6566583B1 (enExample)
EP (4) EP2333071A1 (enExample)
JP (3) JP2002534123A (enExample)
BR (1) BR0008760A (enExample)
CA (1) CA2359629C (enExample)
ES (1) ES2427017T3 (enExample)
HK (1) HK1041292A1 (enExample)
IL (4) IL144269A0 (enExample)
MX (1) MX252154B (enExample)
WO (1) WO2000042195A2 (enExample)

Families Citing this family (79)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6566583B1 (en) * 1997-06-04 2003-05-20 Daniel Facciotti Schizochytrium PKS genes
JP2002537762A (ja) 1999-01-05 2002-11-12 トラスティーズ オブ ボストン ユニバーシティ 改良型核酸クローニング
US7211418B2 (en) * 1999-01-14 2007-05-01 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
US8003772B2 (en) 1999-01-14 2011-08-23 Martek Biosciences Corporation Chimeric PUFA polyketide synthase systems and uses thereof
US20070244192A1 (en) * 1999-01-14 2007-10-18 Martek Biosciences Corporation Plant seed oils containing polyunsaturated fatty acids
US7247461B2 (en) * 1999-01-14 2007-07-24 Martek Biosciences Corporation Nucleic acid molecule encoding ORFA of a PUFA polyketide synthase system and uses thereof
US7217856B2 (en) * 1999-01-14 2007-05-15 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
US7271315B2 (en) * 1999-01-14 2007-09-18 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
ES2352001T3 (es) 2000-01-19 2011-02-14 Martek Biosciences Corporation Procedimiento de extracción sin disolvente.
CN105112463A (zh) 2000-01-28 2015-12-02 Dsmip资产公司 通过在发酵罐中高密度培养真核微生物来增加含有多烯脂肪酸的脂质的产生
TWI337619B (en) * 2001-04-16 2011-02-21 Martek Biosciences Corp Pufa polyketide synthase systems and uses thereof
US20060165735A1 (en) 2002-06-18 2006-07-27 Abril Jesus R Stable emulsions of oils in aqueous solutions and methods for producing same
AU2003296638B2 (en) 2002-12-19 2009-06-11 University Of Bristol Method for the production of polyunsaturated fatty acids
ATE517984T1 (de) 2003-02-27 2011-08-15 Basf Plant Science Gmbh Verfahren zur herstellung mehrfach ungesättigter fettsäuren
US20070082008A1 (en) * 2003-03-07 2007-04-12 Advanced Bionutrition Corporation Feed formulation for terrestrial and aquatic animals
CA2520396C (en) * 2003-03-26 2016-08-09 Martek Biosciences Corporation Pufa polyketide synthase systems and uses thereof
US7855321B2 (en) 2003-03-31 2010-12-21 University Of Bristol Plant acyltransferases specific for long-chained, multiply unsaturated fatty acids
US7646815B2 (en) * 2003-07-15 2010-01-12 Lsi Corporation Intra estimation chroma mode 0 sub-block dependent prediction
US7208590B2 (en) * 2003-07-15 2007-04-24 Abbott Laboratories Genes involved in polyketide synthase pathways and uses thereof
DE102004017370A1 (de) * 2004-04-08 2005-10-27 Nutrinova Nutrition Specialties & Food Ingredients Gmbh PUFA-PKS Gene aus Ulkenia
DE102004017369A1 (de) * 2004-04-08 2005-11-03 Nutrinova Nutrition Specialties & Food Ingredients Gmbh Screeningverfahren zur Identifizierung von PUFA-PKS in Proben
CN103451246B (zh) 2004-04-22 2018-02-16 联邦科学技术研究组织 用重组细胞合成长链多不饱和脂肪酸
CA3023314C (en) 2004-04-22 2019-12-10 Commonwealth Scientific And Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cells
WO2006092449A2 (en) * 2005-03-02 2006-09-08 Metanomics Gmbh Process for the production of fine chemicals
DK2447356T3 (en) 2005-06-07 2016-06-06 Dsm Nutritional Products Ag Eukaryotic MICROORGANISMS FOR PRODUCTION OF LIPIDS AND ANTIOXIDANTS
CA2614095A1 (en) 2005-07-01 2007-01-11 Martek Biosciences Corporation Polyunsaturated fatty acid-containing oil product and uses and production thereof
JP4943678B2 (ja) * 2005-08-01 2012-05-30 国立大学法人北海道大学 細胞の脂肪酸組成を改変する方法およびその利用
CA2630173C (en) 2005-11-18 2018-01-09 Commonwealth Scientific And Industrial Research Organisation Feedstuffs for aquaculture comprising stearidonic acid
KR101524398B1 (ko) * 2006-03-15 2015-06-04 디에스엠 아이피 어셋츠 비.브이. Ｐｕｆａ 폴리케티드 신타제 시스템을 이용한 이종 생물체내 다불포화 지방산의 제조
CN101466270A (zh) * 2006-04-11 2009-06-24 马泰克生物科学公司 包含长链多不饱和脂肪酸的食品及其制备方法
EP2034973B2 (en) 2006-06-22 2022-01-19 DSM IP Assets B.V. Encapsulated labile compound compositions and methods of making the same
WO2008129358A2 (en) * 2006-08-01 2008-10-30 Ocean Nutrition Canada Ltd. Oil producing microbes and methods of modification thereof
EP2059588A4 (en) 2006-08-29 2010-07-28 Commw Scient Ind Res Org FATTY ACID SYNTHESIS
VN24011A1 (en) 2006-08-29 2010-10-25 Martek Biosciences Corp Use of DPA(n-6) oils in infant formula
CA2675026A1 (en) * 2007-01-12 2008-07-24 The Regents Of The University Of Colorado, A Body Corporate Compositions and methods for enhancing tolerance for the production of organic chemicals produced by microorganisms
AU2008269989B2 (en) * 2007-06-29 2014-02-27 Dsm Ip Assets B.V. Production and purification of esters of polyunsaturated fatty acids
JP2010534474A (ja) 2007-07-26 2010-11-11 パシフィック バイオサイエンシーズ オブ カリフォルニア， インコーポレイテッド 分子冗長配列決定
CA2702577A1 (en) 2007-10-15 2009-04-23 Jbs United, Inc. Method for increasing performance of offspring
US8048624B1 (en) 2007-12-04 2011-11-01 Opx Biotechnologies, Inc. Compositions and methods for 3-hydroxypropionate bio-production from biomass
US20110076379A1 (en) * 2008-06-13 2011-03-31 Means Michael M High omega saturated fat free meat products
US8809559B2 (en) 2008-11-18 2014-08-19 Commonwelath Scientific And Industrial Research Organisation Enzymes and methods for producing omega-3 fatty acids
CN102741267B (zh) 2009-03-19 2020-06-23 帝斯曼知识产权资产有限公司 多不饱和脂肪酸合酶核酸分子和多肽，及其组合物、制备方法和用途
US9212371B2 (en) 2009-05-13 2015-12-15 Basf Plant Science Company Gmbh Acyltransferases and uses thereof in fatty acid production
US8809027B1 (en) 2009-09-27 2014-08-19 Opx Biotechnologies, Inc. Genetically modified organisms for increased microbial production of 3-hydroxypropionic acid involving an oxaloacetate alpha-decarboxylase
CA2775390C (en) 2009-09-27 2021-06-29 Opx Biotechnologies, Inc. Method for producing 3-hydroxypropionic acid and other products
CA2781400A1 (en) * 2009-11-20 2011-05-26 Opx Biotechnologies, Inc. Production of an organic acid and/or related chemicals
IN2012DN06617A (enExample) * 2010-01-27 2015-10-23 Opx Biotechnologies Inc
TW201144442A (en) 2010-05-17 2011-12-16 Dow Agrosciences Llc Production of DHA and other LC-PUFAs in plants
CA2801011C (en) * 2010-06-01 2019-02-26 Dsm Ip Assests B.V. Extraction of lipid from cells and products therefrom
AU2011269059B2 (en) 2010-06-25 2016-01-28 Basf Plant Science Company Gmbh Acyltransferases and uses therof in fatty acid production
TW201307553A (zh) 2011-07-26 2013-02-16 Dow Agrosciences Llc 在植物中生產二十二碳六烯酸（ｄｈａ）及其他長鏈多元不飽和脂肪酸（ｌｃ－ｐｕｆａ）之技術
BR122019026149A8 (pt) 2012-06-15 2022-06-28 Commw Scient Ind Res Org Célula hospedeira, processo de produzir um lipídio de planta extraído, método de produzir semente, farinha de semente, método para produzir um produto alimentício, uso e processo para produzir ésteres de etila
US9879234B2 (en) 2012-08-03 2018-01-30 Basf Plant Science Company Gmbh Enzymes, enzyme components and uses thereof
CN109182238A (zh) 2012-08-10 2019-01-11 嘉吉有限公司 用于生产脂肪酸和脂肪酸衍生产物的微生物及方法
EP2970926B1 (en) 2013-03-13 2018-01-31 DSM Nutritional Products AG Engineering microorganisms
WO2014145096A1 (en) 2013-03-15 2014-09-18 Cindy Hoppe Flash evaporation for production purification and recovery
WO2014146026A1 (en) 2013-03-15 2014-09-18 Opx Biotechnologies, Inc. Bioproduction of chemicals
JP6603658B2 (ja) 2013-07-19 2019-11-06 カーギル インコーポレイテッド 脂肪酸及び脂肪酸誘導体の製造のための微生物及び方法
US11408013B2 (en) 2013-07-19 2022-08-09 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
TW201525136A (zh) 2013-11-26 2015-07-01 Dow Agrosciences Llc 利用破囊壺菌ＰＵＦＡ合成酶於油籽作物中生成ω－３長鏈多不飽和脂肪酸
PE20170253A1 (es) 2013-12-18 2017-04-14 Nuseed Pty Ltd Lipido que comprende acidos grasos poliinsaturados de cadena larga
TWI646189B (zh) 2013-12-20 2019-01-01 荷蘭商Ｄｓｍ智慧財產有限公司 用於從微生物細胞獲得微生物油之方法（五）
AU2014369045B2 (en) 2013-12-20 2020-02-27 Dsm Ip Assets B.V. Processes for obtaining microbial oil from microbial cells
NZ721417A (en) 2013-12-20 2022-07-01 Dsm Ip Assets Bv Processes for obtaining microbial oil from microbial cells
CN106029624B (zh) 2013-12-20 2021-12-07 帝斯曼知识产权资产管理有限公司 用于从微生物细胞获得微生物油的方法
AR098896A1 (es) 2013-12-20 2016-06-22 Dsm Ip Assets Bv Proceso para obtener aceite microbiano a partir de células microbianas
US10087430B2 (en) 2014-01-28 2018-10-02 Dsm Ip Assets B.V. Factors for the production and accumulation of polyunsaturated fatty acids (PUFAS) derived from PUFA synthases
WO2015134547A1 (en) 2014-03-03 2015-09-11 Synthetic Genomics, Inc. Molecules associated with fatty acid biosynthetic pathways and uses thereof
AU2015281791A1 (en) 2014-06-27 2017-01-12 Commonwealth Scientific And Industrial Research Organisation Lipid comprising docosapentaenoic acid
EP2993228B1 (en) 2014-09-02 2019-10-09 Cargill, Incorporated Production of fatty acid esters
CA2978770A1 (en) * 2015-03-12 2016-09-15 Synthetic Genomics, Inc. Microorganisms for fatty acid production using elongase and desaturase enzymes
JP6966040B2 (ja) 2016-05-12 2021-11-10 ディーエスエム アイピー アセッツ ビー.ブイ.Dsm Ip Assets B.V. 微細藻類中でのオメガ−３多価不飽和脂肪酸産生を増加させる方法
AR109245A1 (es) 2016-05-12 2018-11-14 Basf Plant Science Co Gmbh Métodos para optimizar la producción de metabolitos en plantas genéticamente modificadas y para procesar estas plantas
WO2018144701A2 (en) 2017-02-02 2018-08-09 Cargill Incorporated Genetically modified cells that produce c6-c10 fatty acid derivatives
US11248244B2 (en) * 2017-05-10 2022-02-15 Nanjing University Of Technology Schizochytrium limacinum strain, building method therefor and application thereof
US11866788B2 (en) 2018-05-08 2024-01-09 Sanford Burnham Prebys Medical Discovery Institute Role of PVT1 in the diagnosis and treatment of MYC-driven cancer
JP7838921B2 (ja) 2018-08-10 2026-04-01 協和発酵バイオ株式会社 エイコサペンタエン酸を生産する微生物及びエイコサペンタエン酸の製造法
US12241101B2 (en) 2018-08-10 2025-03-04 Kyowa Hakko Bio Co., Ltd. Microorganism producing polyunsaturated fatty acid and method for producing polyunsaturated fatty acid
US12516085B2 (en) 2019-09-19 2026-01-06 Sanford Burnham Prebys Medical Discovery Institute Methods and compositions for treating Myc-driven cancers

Family Cites Families (72)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5034322A (en) 1983-01-17 1991-07-23 Monsanto Company Chimeric genes suitable for expression in plant cells
US4792523A (en) 1984-08-31 1988-12-20 Cetus Corporation 3'Expression enhancing fragments and method
US4743548A (en) 1984-09-25 1988-05-10 Calgene, Inc. Plant cell microinjection technique
US5420034A (en) 1986-07-31 1995-05-30 Calgene, Inc. Seed-specific transcriptional regulation
US4943674A (en) 1987-05-26 1990-07-24 Calgene, Inc. Fruit specific transcriptional factors
GB2178752B (en) 1985-07-12 1989-10-11 Unilever Plc Substitute milk fat
US4940835A (en) 1985-10-29 1990-07-10 Monsanto Company Glyphosate-resistant plants
US5068193A (en) 1985-11-06 1991-11-26 Calgene, Inc. Novel method and compositions for introducing alien DNA in vivo
US4795855A (en) 1985-11-14 1989-01-03 Joanne Fillatti Transformation and foreign gene expression with woody species
US5188958A (en) 1986-05-29 1993-02-23 Calgene, Inc. Transformation and foreign gene expression in brassica species
US5565347A (en) 1986-06-10 1996-10-15 Calgene, Inc. Transformation and foreign gene expression with plant species
US5246841A (en) 1986-12-26 1993-09-21 Sagami Chemical Research Center Microbial process for production of eicosapentaenoic acid
AU612133B2 (en) 1987-02-20 1991-07-04 Natinco Nv Production of proteins in active forms
DE3719097C1 (de) 1987-06-06 1988-06-09 Fratzer Uwe Arzneimittel,enthaltend Eicosapentaensaeure und Docosahexaensaeure als ungesaettigte Fettsaeuren sowie Vitamin E
US5011770A (en) 1987-09-04 1991-04-30 Molecular Devices, Inc. DNA detection method
US4999422A (en) 1988-04-15 1991-03-12 Biogen, N.V. Continuous method of refolding proteins
US5565346A (en) 1988-07-27 1996-10-15 Calgene, Inc. Transformation and regeneration system for legumes
US5340742A (en) * 1988-09-07 1994-08-23 Omegatech Inc. Process for growing thraustochytrium and schizochytrium using non-chloride salts to produce a microfloral biomass having omega-3-highly unsaturated fatty acids
US5130242A (en) * 1988-09-07 1992-07-14 Phycotech, Inc. Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids
US5162215A (en) 1988-09-22 1992-11-10 Amgen Inc. Method of gene transfer into chickens and other avian species
EP1103616A3 (en) 1989-02-24 2001-06-27 Monsanto Company Synthetic plant genes and method for preparation
US5106739A (en) 1989-04-18 1992-04-21 Calgene, Inc. CaMv 355 enhanced mannopine synthase promoter and method for using same
US5175095A (en) 1989-07-19 1992-12-29 Calgene, Inc. Ovary-tissue transcriptional factors
US5639790A (en) 1991-05-21 1997-06-17 Calgene, Inc. Plant medium-chain thioesterases
US5798259A (en) * 1992-05-15 1998-08-25 Sagami Chemical Research Center Gene coding for eicosapentaenoic acid synthesizing enzymes and process for production of eicosapentaenoic acid
CA2113557A1 (en) 1992-05-15 1993-11-25 Kazunaga Yazawa Gene which codes for eicosapentaenoic acid synthetase group and process for producing eicosapentaenoic acid
US5683898A (en) 1992-05-15 1997-11-04 Sagami Chemical Research Center Gene coding for eicosapentaenoic acid synthesizing enzymes and process for production of eicosapentaenoic acid
US5310242A (en) * 1992-09-28 1994-05-10 Golder Kimberly A Portable infant seat
DE69424861T2 (de) 1993-02-26 2000-12-28 Calgene Llc., Davis Geminivirus-basiertes-genexpressionsystem
DE4323727A1 (de) * 1993-07-15 1995-03-09 Boehringer Mannheim Gmbh Verfahren zur Identifizierung von menschlichen und tierischen Zellen mit der Fähigkeit zu unbegrenzter Proliferation oder zur Tumorbildung
US5672491A (en) 1993-09-20 1997-09-30 The Leland Stanford Junior University Recombinant production of novel polyketides
AU694181B2 (en) 1994-03-09 1998-07-16 Abbott Laboratories Humanized milk
AU3460195A (en) 1994-08-24 1996-03-14 Meiji Milk Products Co., Ltd. Method of culturing avian cells and cell line obtained thereby
FR2726003B1 (fr) 1994-10-21 2002-10-18 Agronomique Inst Nat Rech Milieu de culture de cellules embryonnaires totipotentes aviaires, procede de culture de ces cellules, et cellules embryonnaires totipotentes aviaires
EP0831149A4 (en) 1995-01-13 2000-12-06 Sagami Chem Res GENES CODING FOR THE GROUP OF ENZYMES FOR BIOSYNTHESIS OF EICOSAPENTIC ACID AND METHOD FOR PRODUCING EICOSAPENTIC ACID
US6582941B1 (en) * 1995-04-17 2003-06-24 Japan As Represented By Director-General Of Agency Of Industrial Science And Technology Microorganisms capable of producing highly unsaturated fatty acids and process for producing highly unsaturated fatty acids by using the microorganisms
GB9508023D0 (en) 1995-04-20 1995-06-07 Scotia Holdings Plc Fatty acid derivatives
EP0913473A4 (en) * 1996-07-10 2000-12-06 Sagami Chem Res METHOD FOR PRODUCING EICOSAPENTIC ACID BY GENETIC RECOMBINATION
US6033883A (en) * 1996-12-18 2000-03-07 Kosan Biosciences, Inc. Production of polyketides in bacteria and yeast
IL132148A0 (en) * 1997-04-11 2001-03-19 Calgene Llc Methods and compositions for synthesis of long chain polyunsaturated fatty acids in plants
US6140486A (en) * 1997-06-04 2000-10-31 Calgene Llc Production of polyunsaturated fatty acids by expression of polyketide-like synthesis genes in plants
US6566583B1 (en) 1997-06-04 2003-05-20 Daniel Facciotti Schizochytrium PKS genes
US6677145B2 (en) * 1998-09-02 2004-01-13 Abbott Laboratories Elongase genes and uses thereof
US8003772B2 (en) * 1999-01-14 2011-08-23 Martek Biosciences Corporation Chimeric PUFA polyketide synthase systems and uses thereof
US7211418B2 (en) * 1999-01-14 2007-05-01 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
US20070244192A1 (en) * 1999-01-14 2007-10-18 Martek Biosciences Corporation Plant seed oils containing polyunsaturated fatty acids
US7247461B2 (en) * 1999-01-14 2007-07-24 Martek Biosciences Corporation Nucleic acid molecule encoding ORFA of a PUFA polyketide synthase system and uses thereof
US7217856B2 (en) * 1999-01-14 2007-05-15 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
US7271315B2 (en) * 1999-01-14 2007-09-18 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
EP1294898B1 (en) * 2000-06-08 2006-08-30 Miami University FATTY ACID ELONGASE 3-KETOACYL CoA SYNTHASE POLYPEPTIDES
US20040010817A1 (en) * 2000-07-21 2004-01-15 Washington State University Research Foundation Plant acyl-CoA synthetases
JP4071622B2 (ja) * 2000-09-28 2008-04-02 バイオリジナル フード アンド サイエンス コーポレーション 脂肪酸不飽和化酵素ファミリーのメンバーであるｆａｄ４、ｆａｄ５、ｆａｄ５−２およびｆａｄ６、ならびにそれらの使用方法
TWI324181B (en) * 2001-04-16 2010-05-01 Martek Biosciences Corp Product and process for transformation of thraustochytriales microorganisms
TWI337619B (en) 2001-04-16 2011-02-21 Martek Biosciences Corp Pufa polyketide synthase systems and uses thereof
US7045683B2 (en) * 2001-05-04 2006-05-16 Abbott Laboratories Δ4-desaturase genes and uses thereof
AU2002309856B2 (en) * 2001-05-14 2008-10-23 Dsm Ip Assets B.V. Production and use of a polar lipid-rich fraction containing omega-3 and/or omega-6 highly unsaturated fatty acids from microbes, genetically modified plant seeds and marine organisms
US20040005672A1 (en) * 2002-02-22 2004-01-08 Santi Daniel V. Heterologous production of polyketides
GB2385852A (en) * 2002-02-27 2003-09-03 Rothamsted Ex Station Delta 6-desaturases from Primulaceae
CA2519169C (en) * 2002-03-16 2013-04-30 The University Of York Transgenic plants expressing enzymes involved in fatty acid biosynthesis
US8084074B2 (en) * 2003-02-12 2011-12-27 E. I. Du Pont De Nemours And Company Production of very long chain polyunsaturated fatty acids in oil seed plants
US20040172682A1 (en) 2003-02-12 2004-09-02 Kinney Anthony J. Production of very long chain polyunsaturated fatty acids in oilseed plants
CA2520396C (en) 2003-03-26 2016-08-09 Martek Biosciences Corporation Pufa polyketide synthase systems and uses thereof
US7855321B2 (en) 2003-03-31 2010-12-21 University Of Bristol Plant acyltransferases specific for long-chained, multiply unsaturated fatty acids
US7125672B2 (en) * 2003-05-07 2006-10-24 E. I. Du Pont De Nemours And Company Codon-optimized genes for the production of polyunsaturated fatty acids in oleaginous yeasts
US7208590B2 (en) * 2003-07-15 2007-04-24 Abbott Laboratories Genes involved in polyketide synthase pathways and uses thereof
DE102004060340A1 (de) 2004-07-16 2006-02-09 Basf Plant Science Gmbh Verfahren zur Erhöhung des Gehalts an mehrfach ungesättigten langkettigen Fettsäuren in transgenen Organismen
EP2339007A1 (en) 2004-09-20 2011-06-29 BASF Plant Science GmbH Arabidopsis genes encoding proteins involved in sugar and lipid metabolism and methods of use
US20080260933A1 (en) * 2004-10-08 2008-10-23 Dow Agroscience Llc Certain Plants with "No Saturate" or Reduced Saturate Levels of Fatty Acids in Seeds, and Oil Derived from the Seeds
DE602005021675D1 (de) * 2004-11-04 2010-07-15 Monsanto Technology Llc Verfahren zur herstellung von ölzusammensetzungen
AR059376A1 (es) * 2006-02-21 2008-03-26 Basf Plant Science Gmbh Procedimiento para la produccion de acidos grasos poliinsaturados
KR101524398B1 (ko) * 2006-03-15 2015-06-04 디에스엠 아이피 어셋츠 비.브이. Ｐｕｆａ 폴리케티드 신타제 시스템을 이용한 이종 생물체내 다불포화 지방산의 제조
AU2008293423B2 (en) * 2007-08-31 2014-10-30 Dsm Ip Assets B.V. Polyunsaturated fatty acid-containing solid fat compositions and uses and production thereof

Also Published As

Publication number Publication date
EP1147197A2 (en) 2001-10-24
MXPA01007153A (es) 2003-07-21
JP2010252811A (ja) 2010-11-11
US20030101486A1 (en) 2003-05-29
BR0008760A (pt) 2002-10-08
EP2333072A1 (en) 2011-06-15
ES2427017T3 (es) 2013-10-28
EP2333071A1 (en) 2011-06-15
US8829274B2 (en) 2014-09-09
JP2002534123A (ja) 2002-10-15
IL144269A0 (en) 2002-05-23
CA2359629A1 (en) 2000-07-20
US20090098622A1 (en) 2009-04-16
US7214853B2 (en) 2007-05-08
US6566583B1 (en) 2003-05-20
WO2000042195A3 (en) 2000-09-28
EP2333073A1 (en) 2011-06-15
IL188590A (en) 2013-06-27
US20110250342A1 (en) 2011-10-13
WO2000042195B1 (en) 2000-11-09
US20080148433A1 (en) 2008-06-19
EP2333072B1 (en) 2013-06-19
HK1159166A1 (en) 2012-07-27
HK1041292A1 (zh) 2002-07-05
WO2000042195A2 (en) 2000-07-20
IL224871A (en) 2017-11-30
MX252154B (es) 2007-12-04
IL188590A0 (en) 2008-04-13
IL144269A (en) 2010-04-15
US20140053299A1 (en) 2014-02-20
US7897844B2 (en) 2011-03-01
JP2008099697A (ja) 2008-05-01

Similar Documents

Publication Publication Date Title
US6566583B1 (en) Schizochytrium PKS genes
US6140486A (en) Production of polyunsaturated fatty acids by expression of polyketide-like synthesis genes in plants
KR101506347B1 (ko) 다불포화 지방산을 함유하는 식물 종자 오일
US7217856B2 (en) PUFA polyketide synthase systems and uses thereof
US7919320B2 (en) PUFA polyketide synthase systems and uses thereof
AU2002303394B2 (en) PUFA polyketide synthase systems and uses thereof
US20070244192A1 (en) Plant seed oils containing polyunsaturated fatty acids
HK1159166B (en) Schizochytrium pks genes
HK1159167A (en) Schitzochytrium pks genes
HK1159168A (en) Schitzochytrium pks genes
HK1162575A (en) Pufa polyketide synthase systems and uses thereof
HK1162576A (en) Pufa polyketide synthase systems and uses thereof
HK1162578A (en) Pufa polyketide synthase systems and uses thereof
HK1162577A (en) Pufa polyketide synthase systems and uses thereof

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20200114