CA2308575A1 - Vecteurs d'expression inductible par hyperthermie utiles pour la therapie genique et procedes d'utilisation de ceux-ci - Google Patents

Vecteurs d'expression inductible par hyperthermie utiles pour la therapie genique et procedes d'utilisation de ceux-ci Download PDF

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Publication number
CA2308575A1
CA2308575A1 CA002308575A CA2308575A CA2308575A1 CA 2308575 A1 CA2308575 A1 CA 2308575A1 CA 002308575 A CA002308575 A CA 002308575A CA 2308575 A CA2308575 A CA 2308575A CA 2308575 A1 CA2308575 A1 CA 2308575A1
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CA
Canada
Prior art keywords
promoter
expression
cell
expression construct
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002308575A
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English (en)
Inventor
Tom Tsang
Eugene Gerner
David T. Harris
Evan Hersh
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Arizona Board of Regents of University of Arizona
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Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2308575A1 publication Critical patent/CA2308575A1/fr
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
    • C12N2830/85—Vector systems having a special element relevant for transcription from vertebrates mammalian
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Epidemiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

L'invention concerne des procédés et des compositions utiles pour exprimer des transgènes dans des cellules cibles. L'invention concerne des produits de recombinaison d'expression utilisant un système d'amplification inductible pour diriger l'expression d'un gène thérapeutique ou d'un autre gène intéressant dans des cellules hôtes de mammifère, ainsi que des procédés utiles à cet effet. L'expression inductible des transgènes à des concentrations élevées dans des conditions physiologiques est la conséquence d'une induction provoquée par des conditions d'hyperthermie par rapport à la température de base des cellules hôtes.
CA002308575A 1997-11-03 1998-11-03 Vecteurs d'expression inductible par hyperthermie utiles pour la therapie genique et procedes d'utilisation de ceux-ci Abandoned CA2308575A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US6408897P 1997-11-03 1997-11-03
US60/064,088 1997-11-03
PCT/US1998/023387 WO1999023216A2 (fr) 1997-11-03 1998-11-03 Vecteurs d'expression inductible par hyperthermie utiles pour la therapie genique et procedes d'utilisation de ceux-ci

Publications (1)

Publication Number Publication Date
CA2308575A1 true CA2308575A1 (fr) 1999-05-14

Family

ID=22053477

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002308575A Abandoned CA2308575A1 (fr) 1997-11-03 1998-11-03 Vecteurs d'expression inductible par hyperthermie utiles pour la therapie genique et procedes d'utilisation de ceux-ci

Country Status (9)

Country Link
EP (1) EP1029041A2 (fr)
JP (1) JP2001521740A (fr)
KR (1) KR20010031762A (fr)
CN (1) CN1299411A (fr)
AU (1) AU1377299A (fr)
BR (1) BR9813917A (fr)
CA (1) CA2308575A1 (fr)
IL (1) IL135943A0 (fr)
WO (1) WO1999023216A2 (fr)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19951765A1 (de) * 1999-10-27 2001-05-03 Thomas Schweder Wirts-Vektor-Systeme zur Überproduktion von thermolabilen Enzymen psychrophiler Organismen
US6579522B1 (en) 2000-06-27 2003-06-17 Genvec, Inc. Replication deficient adenoviral TNF vector
US7285414B2 (en) 2000-09-26 2007-10-23 Emory University Viruses targeted to hypoxic cells and tissues
CA2423833C (fr) * 2000-09-26 2011-02-22 Emory University Virus diriges sur des cellules et des tissus hypoxiques
US20030086903A1 (en) 2001-11-02 2003-05-08 Genvec, Inc. Therapeutic regimen for treating cancer
EP1541674B9 (fr) 2002-06-18 2011-09-14 Eisai R&D Management Co., Ltd. Adipocytes de culture primaire utilises en therapie genique
AU2003278079A1 (en) * 2002-10-14 2004-05-04 Develogen Aktiengesellschaft Fur Entwicklungsbiologische Forschung Proteins involved in the regulation of energy homeostasis
JP2008048601A (ja) * 2004-06-15 2008-03-06 Nippon Kosei Kagaku Kenkyusho:Kk 治療に有用な産物の生産法およびシステム
EP1879622A2 (fr) * 2005-05-03 2008-01-23 Veterinärmedizinische Universität Wien Capsules permeables
RU2499599C2 (ru) * 2007-09-28 2013-11-27 Интрексон Корпорейшн Конструкции терапевтического переключения генов и биореакторы для экспрессии биотерапевтических молекул и их применение
CN103667345B (zh) * 2013-12-02 2015-10-28 东南大学 一种抗肿瘤基因磁性复合纳米颗粒及制备方法
WO2017133175A1 (fr) * 2016-02-04 2017-08-10 Nanjing Legend Biotech Co., Ltd. Cellules mammifères génétiquement modifiées pour thérapie anticancéreuse
CN108913661A (zh) * 2018-06-22 2018-11-30 安徽 一种人体研究用造血干细胞的制备和提取方法
CN114317588B (zh) * 2020-09-29 2023-06-27 中国农业科学院生物技术研究所 一种作物高温响应型基因组精准甲基化调控回路
IT202200000788A1 (it) * 2022-01-19 2023-07-19 Bruno Cell S R L Induzione sensibile alla temperatura del programma miogenico e/o adipogenico in cellule per la produzione di tessuto muscolare e/o adiposo
CN116536349B (zh) * 2023-05-22 2024-02-20 安徽农业大学 一种大豆GmMLP34基因在调控植物耐高温性上的应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3991770A (en) * 1974-01-24 1976-11-16 Leveen Harry H Method for treating benign and malignant tumors utilizing radio frequency, electromagnetic radiation
EP0299127A1 (fr) * 1987-07-16 1989-01-18 IntraCel Corporation Procédé pour exprimer des gènes recombinants, stimulés par une protéine activatrice, exprimée par induction
EP0455424A3 (en) * 1990-05-02 1992-04-29 Merck & Co. Inc. Mammalian inducible promoter cascade system
FR2717187B1 (fr) * 1994-03-10 1996-05-31 Transgene Sa Usage combiné de deux cassettes d'expression pour la production d'une protéine d'intérêt.

Also Published As

Publication number Publication date
EP1029041A2 (fr) 2000-08-23
CN1299411A (zh) 2001-06-13
KR20010031762A (ko) 2001-04-16
AU1377299A (en) 1999-05-24
BR9813917A (pt) 2001-10-30
JP2001521740A (ja) 2001-11-13
WO1999023216A3 (fr) 1999-08-05
WO1999023216A2 (fr) 1999-05-14
IL135943A0 (en) 2001-05-20

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Date Code Title Description
FZDE Discontinued