CA2303891C - Assay sonication apparatus and methodology - Google Patents
Assay sonication apparatus and methodology Download PDFInfo
- Publication number
- CA2303891C CA2303891C CA002303891A CA2303891A CA2303891C CA 2303891 C CA2303891 C CA 2303891C CA 002303891 A CA002303891 A CA 002303891A CA 2303891 A CA2303891 A CA 2303891A CA 2303891 C CA2303891 C CA 2303891C
- Authority
- CA
- Canada
- Prior art keywords
- binding
- cell
- recited
- assay
- sonication
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000003556 assay Methods 0.000 title claims abstract description 134
- 238000000527 sonication Methods 0.000 title claims description 216
- 238000000034 method Methods 0.000 title claims description 44
- 239000003153 chemical reaction reagent Substances 0.000 claims description 204
- 238000009739 binding Methods 0.000 claims description 129
- 239000007790 solid phase Substances 0.000 claims description 83
- 230000027455 binding Effects 0.000 claims description 78
- 239000007787 solid Substances 0.000 claims description 21
- 238000000159 protein binding assay Methods 0.000 claims description 20
- 239000012491 analyte Substances 0.000 claims description 19
- 238000001514 detection method Methods 0.000 claims description 18
- 238000005259 measurement Methods 0.000 claims description 16
- 239000000203 mixture Substances 0.000 claims description 14
- 238000004140 cleaning Methods 0.000 claims description 8
- 238000003018 immunoassay Methods 0.000 claims description 8
- 238000007899 nucleic acid hybridization Methods 0.000 claims description 5
- 102000004190 Enzymes Human genes 0.000 claims description 4
- 108090000790 Enzymes Proteins 0.000 claims description 4
- 238000000670 ligand binding assay Methods 0.000 claims description 4
- 108020004707 nucleic acids Proteins 0.000 claims description 2
- 102000039446 nucleic acids Human genes 0.000 claims description 2
- 150000007523 nucleic acids Chemical class 0.000 claims description 2
- 102000008394 Immunoglobulin Fragments Human genes 0.000 claims 1
- 108010021625 Immunoglobulin Fragments Proteins 0.000 claims 1
- 238000004020 luminiscence type Methods 0.000 claims 1
- 102000005962 receptors Human genes 0.000 claims 1
- 108020003175 receptors Proteins 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 description 92
- 210000004027 cell Anatomy 0.000 description 83
- 239000000243 solution Substances 0.000 description 37
- 239000000463 material Substances 0.000 description 32
- 239000000523 sample Substances 0.000 description 24
- 239000002131 composite material Substances 0.000 description 23
- 238000010168 coupling process Methods 0.000 description 20
- 238000005859 coupling reaction Methods 0.000 description 20
- 239000007788 liquid Substances 0.000 description 20
- 230000008878 coupling Effects 0.000 description 19
- 230000001965 increasing effect Effects 0.000 description 18
- 238000002405 diagnostic procedure Methods 0.000 description 14
- 238000002156 mixing Methods 0.000 description 14
- 239000000126 substance Substances 0.000 description 12
- 230000005540 biological transmission Effects 0.000 description 11
- 230000006870 function Effects 0.000 description 11
- 239000012780 transparent material Substances 0.000 description 10
- 239000012911 assay medium Substances 0.000 description 8
- 238000010586 diagram Methods 0.000 description 8
- 230000008569 process Effects 0.000 description 7
- 230000007423 decrease Effects 0.000 description 6
- 230000002452 interceptive effect Effects 0.000 description 6
- 229920000642 polymer Polymers 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 238000012546 transfer Methods 0.000 description 6
- 238000002525 ultrasonication Methods 0.000 description 6
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 5
- 230000004913 activation Effects 0.000 description 5
- 239000006227 byproduct Substances 0.000 description 5
- 239000000356 contaminant Substances 0.000 description 5
- 230000005284 excitation Effects 0.000 description 5
- 238000003780 insertion Methods 0.000 description 5
- 230000037431 insertion Effects 0.000 description 5
- 230000003287 optical effect Effects 0.000 description 5
- 102100024342 Contactin-2 Human genes 0.000 description 4
- 229910021607 Silver chloride Inorganic materials 0.000 description 4
- 101000690440 Solanum lycopersicum Floral homeotic protein AGAMOUS Proteins 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 210000004188 enterochromaffin-like cell Anatomy 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 229920002457 flexible plastic Polymers 0.000 description 4
- 238000011534 incubation Methods 0.000 description 4
- 238000003367 kinetic assay Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 229920003023 plastic Polymers 0.000 description 4
- 239000004033 plastic Substances 0.000 description 4
- 230000001681 protective effect Effects 0.000 description 4
- HKZLPVFGJNLROG-UHFFFAOYSA-M silver monochloride Chemical compound [Cl-].[Ag+] HKZLPVFGJNLROG-UHFFFAOYSA-M 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 238000013459 approach Methods 0.000 description 3
- 238000002820 assay format Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 239000004020 conductor Substances 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- 238000009792 diffusion process Methods 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 230000001939 inductive effect Effects 0.000 description 3
- 230000000670 limiting effect Effects 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 229920001200 poly(ethylene-vinyl acetate) Polymers 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- YFTHZRPMJXBUME-UHFFFAOYSA-N tripropylamine Chemical compound CCCN(CCC)CCC YFTHZRPMJXBUME-UHFFFAOYSA-N 0.000 description 3
- 239000011800 void material Substances 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- 239000003298 DNA probe Substances 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 2
- 150000001485 argon Chemical class 0.000 description 2
- 239000012131 assay buffer Substances 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 125000002843 carboxylic acid group Chemical group 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 238000007405 data analysis Methods 0.000 description 2
- 238000013480 data collection Methods 0.000 description 2
- 238000003795 desorption Methods 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000000835 electrochemical detection Methods 0.000 description 2
- 238000003487 electrochemical reaction Methods 0.000 description 2
- 238000003366 endpoint assay Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000003384 imaging method Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 230000009149 molecular binding Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000011253 protective coating Substances 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 238000003260 vortexing Methods 0.000 description 2
- BZSVVCFHMVMYCR-UHFFFAOYSA-N 2-pyridin-2-ylpyridine;ruthenium Chemical group [Ru].N1=CC=CC=C1C1=CC=CC=N1.N1=CC=CC=C1C1=CC=CC=N1.N1=CC=CC=C1C1=CC=CC=N1 BZSVVCFHMVMYCR-UHFFFAOYSA-N 0.000 description 1
- 108020003215 DNA Probes Proteins 0.000 description 1
- 238000012286 ELISA Assay Methods 0.000 description 1
- NQTADLQHYWFPDB-UHFFFAOYSA-N N-Hydroxysuccinimide Chemical compound ON1C(=O)CCC1=O NQTADLQHYWFPDB-UHFFFAOYSA-N 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 229920001054 Poly(ethylene‐co‐vinyl acetate) Polymers 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000005534 acoustic noise Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 239000002981 blocking agent Substances 0.000 description 1
- 239000008366 buffered solution Substances 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- KRVSOGSZCMJSLX-UHFFFAOYSA-L chromic acid Substances O[Cr](O)(=O)=O KRVSOGSZCMJSLX-UHFFFAOYSA-L 0.000 description 1
- 230000009137 competitive binding Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 239000012611 container material Substances 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000013016 damping Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000007812 electrochemical assay Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 230000005281 excited state Effects 0.000 description 1
- 230000009969 flowable effect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- AWJWCTOOIBYHON-UHFFFAOYSA-N furo[3,4-b]pyrazine-5,7-dione Chemical compound C1=CN=C2C(=O)OC(=O)C2=N1 AWJWCTOOIBYHON-UHFFFAOYSA-N 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000003842 industrial chemical process Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012806 monitoring device Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- WLVXZMHBUFGVMK-UHFFFAOYSA-N n-ethyl-n'-(2-methylbutan-2-yl)methanediimine Chemical compound CCN=C=NC(C)(C)CC WLVXZMHBUFGVMK-UHFFFAOYSA-N 0.000 description 1
- 230000009871 nonspecific binding Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 239000012429 reaction media Substances 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000027756 respiratory electron transport chain Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000002198 surface plasmon resonance spectroscopy Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
- 238000006276 transfer reaction Methods 0.000 description 1
- 238000004506 ultrasonic cleaning Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
- G01N33/5438—Electrodes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/808—Optical sensing apparatus
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/805—Optical property
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/932,985 | 1997-09-18 | ||
| US08/932,985 US6413783B1 (en) | 1997-09-18 | 1997-09-18 | Assay sonication apparatus and methodology |
| PCT/US1998/019118 WO1999014599A1 (en) | 1997-09-18 | 1998-09-11 | Assay sonication apparatus and methodology |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2303891A1 CA2303891A1 (en) | 1999-03-25 |
| CA2303891C true CA2303891C (en) | 2009-11-17 |
Family
ID=25463245
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002303891A Expired - Fee Related CA2303891C (en) | 1997-09-18 | 1998-09-11 | Assay sonication apparatus and methodology |
Country Status (7)
Families Citing this family (86)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6207369B1 (en) * | 1995-03-10 | 2001-03-27 | Meso Scale Technologies, Llc | Multi-array, multi-specific electrochemiluminescence testing |
| US6673533B1 (en) * | 1995-03-10 | 2004-01-06 | Meso Scale Technologies, Llc. | Multi-array multi-specific electrochemiluminescence testing |
| US7687039B2 (en) | 1998-10-28 | 2010-03-30 | Covaris, Inc. | Methods and systems for modulating acoustic energy delivery |
| US7981368B2 (en) | 1998-10-28 | 2011-07-19 | Covaris, Inc. | Method and apparatus for acoustically controlling liquid solutions in microfluidic devices |
| ATE381016T1 (de) * | 1998-10-28 | 2007-12-15 | Covaris Inc | Vorrichtung und verfahren zur kontrolle einer akustischen behandlung |
| US6948843B2 (en) * | 1998-10-28 | 2005-09-27 | Covaris, Inc. | Method and apparatus for acoustically controlling liquid solutions in microfluidic devices |
| EP1128185B8 (en) * | 2000-02-25 | 2009-08-19 | Hitachi, Ltd. | Mixing device for automatic analyzer |
| US20040209303A1 (en) | 2000-10-03 | 2004-10-21 | Martin Mark T. | Methods and compositions for directed microwave chemistry |
| US7351590B2 (en) * | 2000-10-03 | 2008-04-01 | Mirari Biosciences, Inc. | Methods and compositions for directed microwave chemistry |
| US7348182B2 (en) * | 2000-10-03 | 2008-03-25 | Mirari Biosciences, Inc. | Directed microwave chemistry |
| WO2002084754A2 (en) * | 2000-10-25 | 2002-10-24 | Washington State University Research Foundation | Piezoelectric micro-transducers, methods of use and manufacturing methods for same |
| GB0103765D0 (en) * | 2001-02-15 | 2001-04-04 | Affitech As | Assay |
| EP2423673B8 (en) | 2001-06-29 | 2020-10-28 | Meso Scale Technologies, LLC. | Apparatus for measuring luminescence from a multi-well assay plate having a plurality of wells, method of measuring luminescence using the apparatus and system comprising the apparatus |
| AU2020204526B2 (en) * | 2001-06-29 | 2022-04-07 | Meso Scale Technologies, Llc. | Assay plates reader systems and methods for luminescence test measurements |
| ATE471369T1 (de) | 2001-07-30 | 2010-07-15 | Meso Scale Technologies Llc | Assay-elektroden mit schichten aus immobilisiertem lipid/protein sowie verfahren zu deren herstellung und verwendung |
| EP1451348B1 (en) | 2001-09-10 | 2015-08-12 | Meso Scale Technologies, LLC | Methods, reagents, kits and apparatus for protein function analysis |
| US6939032B2 (en) * | 2001-10-25 | 2005-09-06 | Erie Scientific Company | Cover slip mixing apparatus |
| US6838888B2 (en) * | 2001-12-13 | 2005-01-04 | Agilent Technologies, Inc. | Flow cell humidity sensor system |
| US20040066703A1 (en) * | 2002-10-03 | 2004-04-08 | Protasis Corporation | Fluid-handling apparatus and methods |
| US7000870B2 (en) * | 2002-11-07 | 2006-02-21 | Aerion Corporation | Laminar flow wing for transonic cruise |
| US7341837B2 (en) * | 2003-09-02 | 2008-03-11 | Lawton Robert L | Soluble analyte detection and amplification |
| US7981362B2 (en) | 2003-11-04 | 2011-07-19 | Meso Scale Technologies, Llc | Modular assay plates, reader systems and methods for test measurements |
| US7328628B2 (en) | 2003-12-08 | 2008-02-12 | Covaris, Inc. | Apparatus and methods for sample preparation |
| EP1628136A1 (en) * | 2004-08-19 | 2006-02-22 | B.R.A.H.M.S. Aktiengesellschaft | Method of diagnosis of disease using copeptin |
| JP4469928B2 (ja) * | 2004-09-22 | 2010-06-02 | ベックマン・コールター・インコーポレーテッド | 攪拌容器 |
| JP4365813B2 (ja) * | 2004-09-22 | 2009-11-18 | オリンパス株式会社 | 攪拌装置、容器および攪拌装置を備えた分析装置 |
| DE602005027127D1 (de) * | 2004-12-23 | 2011-05-05 | Hoffmann La Roche | Nachweis eines therapeutischen antikörpers in einem versuchstier |
| GB2426050A (en) | 2005-05-09 | 2006-11-15 | Orion Diagnostica Oy | Method of measuring binding rate using sonication |
| WO2007016605A2 (en) * | 2005-08-01 | 2007-02-08 | Covaris, Inc. | An apparatus and a method for processing a sample using acoustic energy |
| US7372562B2 (en) * | 2005-10-17 | 2008-05-13 | Hewlett-Packard Development Company, L.P. | Dynamic random separation among nanoparticles for nano enhanced Raman spectroscopy (NERS) molecular sensing |
| US7342656B2 (en) * | 2005-10-17 | 2008-03-11 | Hewlett-Packard Development Company, L.P. | Dynamically variable separation among nanoparticles for nano-enhanced Raman spectroscopy (NERS) molecular sensing |
| US20070292844A1 (en) * | 2006-03-22 | 2007-12-20 | Northrop Grumman Corporation | Enhanced biohazard detection system |
| WO2008016691A2 (en) * | 2006-08-01 | 2008-02-07 | Covaris, Inc. | Methods and apparatus for treating samples with acoustic energy |
| EP1925359A1 (en) | 2006-11-22 | 2008-05-28 | Covaris, Inc. | Methods and apparatus for treating samples with acoustic energy to form particles and particulates |
| DE102007017450A1 (de) * | 2007-04-02 | 2008-10-09 | Niro-Plan Ag | Verfahren und Vorrichtung zur Herstellung von Caffe Latte Macchiato |
| KR101833297B1 (ko) | 2008-04-11 | 2018-02-28 | 메소 스케일 테크놀러지즈, 엘엘시 | 분석 장치, 방법 및 시약 |
| US20100008178A1 (en) * | 2008-07-14 | 2010-01-14 | Dale Fahrion | Acoustic Beverage Mixer |
| US20100261292A1 (en) | 2009-04-10 | 2010-10-14 | Meso Scale Technologies, Llc | Methods for Conducting Assays |
| CA3083798C (en) * | 2009-07-27 | 2023-08-15 | Meso Scale Technologies, Llc | Assay apparatuses, consumables and methods |
| BR112012033163A2 (pt) | 2010-08-17 | 2016-10-11 | Hoffmann La Roche | ''anticorpos monoclonais, linhagens celulares, usos de um anticorpo, kit, métodos para detectar um anticorpo, composição de anticorpo e uso de uma composição de anticorpo'' |
| CA3241642A1 (en) | 2010-10-14 | 2012-04-19 | Meso Scale Technologies, Llc | Reagent storage in an assay device |
| JP6071886B2 (ja) | 2010-10-14 | 2017-02-01 | ザ・ジョンズ・ホプキンス・ユニバーシティ | 脳損傷のバイオマーカー |
| CN103443614B (zh) | 2010-10-25 | 2015-07-22 | 霍夫曼-拉罗奇有限公司 | 信号增强化合物在电化学发光检测中的用途 |
| US8459121B2 (en) | 2010-10-28 | 2013-06-11 | Covaris, Inc. | Method and system for acoustically treating material |
| US8709359B2 (en) | 2011-01-05 | 2014-04-29 | Covaris, Inc. | Sample holder and method for treating sample material |
| JP6087293B2 (ja) | 2011-01-06 | 2017-03-01 | メソ スケール テクノロジーズ エルエルシー | アッセイカートリッジ及びその使用方法 |
| US20140141458A1 (en) | 2011-05-12 | 2014-05-22 | The Johns Hopkins University | Assay reagents for a neurogranin diagnostic kit |
| DK2776833T3 (da) | 2011-11-11 | 2019-01-02 | Eli N Glezer | Cobinder-understøttet testfremgangsmåde |
| US9213043B2 (en) | 2012-05-15 | 2015-12-15 | Wellstat Diagnostics, Llc | Clinical diagnostic system including instrument and cartridge |
| US9081001B2 (en) | 2012-05-15 | 2015-07-14 | Wellstat Diagnostics, Llc | Diagnostic systems and instruments |
| US9625465B2 (en) | 2012-05-15 | 2017-04-18 | Defined Diagnostics, Llc | Clinical diagnostic systems |
| CA3107353C (en) | 2013-01-04 | 2024-05-28 | Meso Scale Technologies, Llc. | Assay apparatuses, methods and reagents |
| US10545161B2 (en) | 2013-03-11 | 2020-01-28 | Cue Health Inc. | Systems and methods for detection and quantification of analytes |
| US9789483B2 (en) | 2013-03-11 | 2017-10-17 | Cue Inc. | System for portable and easy-to-use detection of analytes with mobile computing device |
| US9623409B2 (en) | 2013-03-11 | 2017-04-18 | Cue Inc. | Cartridges, kits, and methods for enhanced mixing for detection and quantification of analytes |
| KR20250002843A (ko) | 2013-03-11 | 2025-01-07 | 메소 스케일 테크놀러지즈, 엘엘시 | 다중화 어세이를 수행하기 위한 개선된 방법 |
| AU2014248759B2 (en) | 2013-03-13 | 2020-02-27 | Meso Scale Technologies, Llc. | Improved assay methods |
| US10114015B2 (en) | 2013-03-13 | 2018-10-30 | Meso Scale Technologies, Llc. | Assay methods |
| EP2970631B1 (en) | 2013-03-15 | 2017-05-03 | Lubrizol Advanced Materials, Inc. | Heavy metal free cpvc compositions |
| WO2015009907A1 (en) | 2013-07-17 | 2015-01-22 | The Johns Hopkins University | A multi-protein biomarker assay for brain injury detection and outcome |
| US10300453B2 (en) * | 2013-10-16 | 2019-05-28 | University Of Iowa Research Foundation | Thin layer sonochemistry and sonoelectrochemistry devices and methods |
| JP6410422B2 (ja) * | 2013-11-25 | 2018-10-24 | 株式会社タウンズ | タンパク質検出装置およびタンパク質検出方法 |
| CA3229509A1 (en) | 2014-05-09 | 2015-11-12 | Meso Scale Technologies, Llc. | Graphene-modified electrodes |
| USD745423S1 (en) | 2014-05-12 | 2015-12-15 | Cue Inc. | Automated analyzer test cartridge and sample collection device for analyte detection |
| JP6695280B2 (ja) | 2014-05-15 | 2020-05-20 | メソ スケール テクノロジーズ エルエルシー | 改善されたアッセイ方法 |
| WO2016044697A1 (en) | 2014-09-19 | 2016-03-24 | The Johns Hopkins University | Biomarkers of cognitive dysfunction |
| WO2016134365A1 (en) | 2015-02-20 | 2016-08-25 | The Johns Hopkins University | Biomarkers of myocardial injury |
| US10509045B2 (en) | 2015-05-29 | 2019-12-17 | University Of Florida Research Foundation, Incorporated | Methods for diagnosing Huntington's disease |
| CA2992596C (en) | 2015-07-17 | 2023-08-22 | Cue Inc. | Systems and methods for enhanced detection and quantification of analytes |
| EP3173776B1 (en) * | 2015-11-25 | 2019-01-16 | C A Casyso GmbH | System and method for mixing and testing a liquid |
| CN108698047B (zh) | 2016-03-11 | 2022-04-01 | 豪夫迈·罗氏有限公司 | 电化学发光检测中的支链胺 |
| JP7509394B2 (ja) | 2016-04-04 | 2024-07-02 | ユニバーシティ オブ フロリダ リサーチ ファンデーション インコーポレーティッド | 反復関連非ａｔｇ（ｒａｎ）翻訳を調節するためのｅｉｆ３の操作 |
| US11237161B2 (en) | 2017-01-25 | 2022-02-01 | Cue Health Inc. | Systems and methods for enhanced detection and quantification of analytes |
| EP3634573A4 (en) * | 2017-04-24 | 2021-01-27 | Abdolahad, Mohammad | ULTRASONIC ASSISTED ELECTROCHEMICAL DISTINCTION OF NORMAL AND CARCINOUS CELLS |
| US11903910B2 (en) | 2017-09-26 | 2024-02-20 | University Of Florida Research Foundation, Incorporated | Use of metformin and analogs thereof to reduce RAN protein levels in the treatment of neurological disorders |
| EP4310184A3 (en) | 2018-02-23 | 2024-05-01 | Meso Scale Technologies, LLC. | Methods of screening antigen-binding molecules by normalizing for the concentration of antigen-binding molecule |
| WO2019236632A1 (en) | 2018-06-04 | 2019-12-12 | Avon Products. Inc. | Protein biomarkers for identifying and treating aging skin and skin conditions |
| AU2019418498A1 (en) | 2019-01-03 | 2021-07-15 | Meso Scale Technologies, Llc | Compositions and methods for carrying out assay measurements |
| US20220099661A1 (en) | 2019-03-01 | 2022-03-31 | Meso Scale Technologies, Llc. | Electrochemiluminescent labeled probes for use in immunoassay methods, methods using such and kits comprising same |
| JP2022535580A (ja) | 2019-06-07 | 2022-08-09 | エフ．ホフマン－ラ ロシュ アーゲー | 全ｌｎａオリゴヌクレオチドのハイブリダイズ |
| WO2021236584A1 (en) | 2020-05-19 | 2021-11-25 | Meso Scale Technologies, Llc. | Methods, compositions, and kits for nucleic acid detection |
| US20220018785A1 (en) | 2020-07-01 | 2022-01-20 | Meso Scale Technologies, Llc. | Compositions and methods for assay measurements |
| EP4222498A4 (en) | 2020-09-29 | 2024-10-16 | The Johns Hopkins University | INTEGRATED PROTEOMIC BIOMARKERS FOR THE DETECTION OF AGGRESSIVE PROSTATE CANCER |
| JP2023554517A (ja) | 2020-12-22 | 2023-12-27 | エフ． ホフマン－ラ ロシュ アーゲー | 試料中の目的の分析物を検出するための方法 |
| EP4457359A1 (en) | 2021-12-30 | 2024-11-06 | Meso Scale Technologies, LLC | Methods for electrochemiluminescence detection |
| JP2025506652A (ja) | 2022-02-18 | 2025-03-13 | エフ． ホフマン－ラ ロシュ アーゲー | 試料中の目的の分析物を検出するための方法 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1063486A (en) * | 1976-04-22 | 1979-10-02 | Bristol-Myers Squibb Company | Ultrasonic cleaner |
| US4575485A (en) * | 1983-08-29 | 1986-03-11 | Syntex (U.S.A.) Inc. | Ultrasonic enhanced immuno-reactions |
| US4983523A (en) * | 1988-04-08 | 1991-01-08 | Gene-Trak Systems | Methods for preparing sample nucleic acids for hybridization |
| JPH02312591A (ja) * | 1989-05-26 | 1990-12-27 | Chisso Corp | 超音波による酵素反応の制御法 |
| US5247954A (en) * | 1991-11-12 | 1993-09-28 | Submicron Systems, Inc. | Megasonic cleaning system |
| US5639423A (en) | 1992-08-31 | 1997-06-17 | The Regents Of The University Of Calfornia | Microfabricated reactor |
| US5866434A (en) * | 1994-12-08 | 1999-02-02 | Meso Scale Technology | Graphitic nanotubes in luminescence assays |
| US6207369B1 (en) * | 1995-03-10 | 2001-03-27 | Meso Scale Technologies, Llc | Multi-array, multi-specific electrochemiluminescence testing |
| US5589136A (en) | 1995-06-20 | 1996-12-31 | Regents Of The University Of California | Silicon-based sleeve devices for chemical reactions |
-
1997
- 1997-09-18 US US08/932,985 patent/US6413783B1/en not_active Expired - Lifetime
-
1998
- 1998-09-11 AT AT98951916T patent/ATE405835T1/de active
- 1998-09-11 AU AU97745/98A patent/AU9774598A/en not_active Abandoned
- 1998-09-11 WO PCT/US1998/019118 patent/WO1999014599A1/en active Application Filing
- 1998-09-11 DE DE69839911T patent/DE69839911D1/de not_active Expired - Lifetime
- 1998-09-11 CA CA002303891A patent/CA2303891C/en not_active Expired - Fee Related
- 1998-09-11 EP EP98951916A patent/EP1018015B1/en not_active Expired - Lifetime
-
2002
- 2002-05-21 US US10/152,234 patent/US7008796B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP1018015A1 (en) | 2000-07-12 |
| US7008796B2 (en) | 2006-03-07 |
| WO1999014599A1 (en) | 1999-03-25 |
| US20020137234A1 (en) | 2002-09-26 |
| US6413783B1 (en) | 2002-07-02 |
| EP1018015A4 (en) | 2005-10-19 |
| EP1018015B1 (en) | 2008-08-20 |
| DE69839911D1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 2008-10-02 |
| CA2303891A1 (en) | 1999-03-25 |
| ATE405835T1 (de) | 2008-09-15 |
| AU9774598A (en) | 1999-04-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2303891C (en) | Assay sonication apparatus and methodology | |
| JP3053112B2 (ja) | 粒子濃縮と化学発光検出を利用する改良発光検定のための方法ならびに装置 | |
| US5746974A (en) | Apparatus for improved luminescence assays using particle concentration, electrochemical generation of chemiluminescence and chemiluminescence detection | |
| US8541174B2 (en) | Multi-array, multi-specific electrochemiluminescence testing | |
| JP4754003B2 (ja) | 多重アレイの多重特異的な電気化学発光試験 | |
| US6448091B1 (en) | Method and apparatus for improved luminescence assays using particle concentration chemiluminescence detection | |
| US5798083A (en) | Apparatus for improved luminescence assays using particle concentration and chemiluminescence detection | |
| Kurita et al. | Development of electrogenerated chemiluminescence-based enzyme linked immunosorbent assay for sub-pM detection | |
| US20140274805A1 (en) | Multi-array, multi-specific electrochemiluminescence testing | |
| US20030008339A1 (en) | Methods and apparatus for improved luminescence assays | |
| WO1996028538A9 (en) | Multi-array, multi-specific electrochemiluminescence testing | |
| US5770459A (en) | Methods and apparatus for improved luminescence assays using particle concentration, electrochemical generation of chemiluminescence detection | |
| Namba et al. | Highly sensitive electrochemiluminescence immunoassay using the ruthenium chelate-labeled antibody bound on the magnetic micro beads | |
| US7931788B1 (en) | Method and apparatus for the detection of pathogens, parasites, and toxins | |
| CN105452851A (zh) | 检测液体样本中的分析物的电致化学发光方法以及分析系统 | |
| JP3641100B2 (ja) | 電気化学発光検出セル | |
| CN102565381B (zh) | 导电微粒子介导电化学发光信号的免疫检测方法 | |
| WO1996021154A1 (en) | Electrogenerated chemiluminescence through enhanced particle luminescence | |
| US9880123B2 (en) | Chip for a pathogens, parasites, toxins and desired chemical compounds detection | |
| AU676665C (en) | Methods and apparatus for improved luminescence assays usingparticle concentration and chemiluminescence detection |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |
Effective date: 20180911 |