CA2273064C - Novel processes for amplifying nucleic acid, post-termination labeling process for nucleic acid sequencing and producing nucleic acid having decreased thermodynamic stability - Google Patents

Novel processes for amplifying nucleic acid, post-termination labeling process for nucleic acid sequencing and producing nucleic acid having decreased thermodynamic stability Download PDF

Info

Publication number
CA2273064C
CA2273064C CA002273064A CA2273064A CA2273064C CA 2273064 C CA2273064 C CA 2273064C CA 002273064 A CA002273064 A CA 002273064A CA 2273064 A CA2273064 A CA 2273064A CA 2273064 C CA2273064 C CA 2273064C
Authority
CA
Canada
Prior art keywords
nucleic acid
segment
primer
acid sequence
template
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA002273064A
Other languages
English (en)
French (fr)
Other versions
CA2273064A1 (en
Inventor
Elazar Rabbani
Jannis G. Stavrianopoulos
James J. Donegan
Jack Coleman
Marleen Walner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Enzo Diagnostics Inc
Original Assignee
Enzo Diagnostics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22298492&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2273064(C) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Enzo Diagnostics Inc filed Critical Enzo Diagnostics Inc
Priority to CA002475018A priority Critical patent/CA2475018C/en
Publication of CA2273064A1 publication Critical patent/CA2273064A1/en
Application granted granted Critical
Publication of CA2273064C publication Critical patent/CA2273064C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
CA002273064A 1998-06-24 1999-06-15 Novel processes for amplifying nucleic acid, post-termination labeling process for nucleic acid sequencing and producing nucleic acid having decreased thermodynamic stability Expired - Lifetime CA2273064C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA002475018A CA2475018C (en) 1998-06-24 1999-06-15 Novel processes for amplifying nucleic acid, post-termination labeling process for nucleic acid sequencing and producing nucleic acid having decreased thermodynamic stability

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/104,067 US6743605B1 (en) 1998-06-24 1998-06-24 Linear amplification of specific nucleic acid sequences
US09/104,067 1998-06-24

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA002475018A Division CA2475018C (en) 1998-06-24 1999-06-15 Novel processes for amplifying nucleic acid, post-termination labeling process for nucleic acid sequencing and producing nucleic acid having decreased thermodynamic stability

Publications (2)

Publication Number Publication Date
CA2273064A1 CA2273064A1 (en) 1999-12-24
CA2273064C true CA2273064C (en) 2008-04-01

Family

ID=22298492

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002273064A Expired - Lifetime CA2273064C (en) 1998-06-24 1999-06-15 Novel processes for amplifying nucleic acid, post-termination labeling process for nucleic acid sequencing and producing nucleic acid having decreased thermodynamic stability

Country Status (6)

Country Link
US (11) US6743605B1 (OSRAM)
EP (1) EP0971039B1 (OSRAM)
JP (3) JP2000037194A (OSRAM)
CA (1) CA2273064C (OSRAM)
DE (1) DE69943292D1 (OSRAM)
IL (1) IL130477A (OSRAM)

Families Citing this family (110)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994003624A1 (en) 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
US6261808B1 (en) 1992-08-04 2001-07-17 Replicon, Inc. Amplification of nucleic acid molecules via circular replicons
GB9812768D0 (en) * 1998-06-13 1998-08-12 Zeneca Ltd Methods
US8445664B2 (en) 1998-06-24 2013-05-21 Enzo Diagnostics, Inc. Kits for amplifying and detecting nucleic acid sequences
US6743605B1 (en) * 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
JP3974441B2 (ja) * 1998-11-09 2007-09-12 栄研化学株式会社 核酸の合成方法
WO2000028082A1 (fr) 1998-11-09 2000-05-18 Eiken Kagaku Kabushiki Kaisha Procede de synthese d'acide nucleique
US6692918B2 (en) 1999-09-13 2004-02-17 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
DE60025840T2 (de) 1999-11-08 2006-07-27 Eiken Kagaku K.K. Methode zum nachweis von variationen oder polymorphismen
JP4721603B2 (ja) * 1999-11-08 2011-07-13 栄研化学株式会社 変異および/または多型の検出方法
AU2001244677A1 (en) 2000-04-07 2001-10-23 Eiken Kagaku Kabushiki Kaisha Method of amplifying nucleic acid by using double-stranded nucleic acid as template
JP2001309785A (ja) * 2000-05-01 2001-11-06 Eiken Chem Co Ltd 遺伝子配列間の相違検出方法
US7846733B2 (en) 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
ATE455186T1 (de) 2000-06-26 2010-01-15 Nugen Technologies Inc Methoden und zusammensetzungen zur auf transkription basierenden vervielfältigung von nukleinsäuren
WO2002024902A1 (en) 2000-09-19 2002-03-28 Eiken Kagaku Kabushiki Kaisha Method of synthesizing polynucleotide
EP1427847B1 (en) 2000-12-13 2010-07-28 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
BR0205268A (pt) 2001-03-09 2004-11-30 Nugen Technologies Inc Processos e composições para a mplificação de sequências de rna
CA2439074A1 (en) * 2001-03-09 2002-09-19 Nugen Technologies, Inc. Methods and compositions for amplification of rna sequences
US20040161741A1 (en) 2001-06-30 2004-08-19 Elazar Rabani Novel compositions and processes for analyte detection, quantification and amplification
US9777312B2 (en) 2001-06-30 2017-10-03 Enzo Life Sciences, Inc. Dual polarity analysis of nucleic acids
US9261460B2 (en) 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
JP2005052002A (ja) * 2001-07-06 2005-03-03 Wakunaga Pharmaceut Co Ltd 非環状核酸断片の増幅方法
US20110151438A9 (en) 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
WO2003044216A2 (en) 2001-11-19 2003-05-30 Parallele Bioscience, Inc. Multiplex oligonucleotide addition and target amplification
US9353405B2 (en) 2002-03-12 2016-05-31 Enzo Life Sciences, Inc. Optimized real time nucleic acid detection processes
US6815167B2 (en) 2002-04-25 2004-11-09 Geneohm Sciences Amplification of DNA to produce single-stranded product of defined sequence and length
EP2292801B1 (en) 2002-06-14 2018-08-08 Gen-Probe Incorporated Compositions and methods for detecting hepatitis b virus
GB0218215D0 (en) * 2002-08-06 2002-09-11 Tepnel Medical Ltd Amplification of nucleic acids
TW200413527A (en) 2002-10-29 2004-08-01 Riken Amplification of nucleic acid
EP1613778B1 (en) * 2003-04-14 2014-11-26 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
EP1712618B1 (en) * 2003-12-25 2014-06-04 Riken Method of amplifying nucleic acid and method of detecting mutated nucleic acid using the same
US7575863B2 (en) 2004-05-28 2009-08-18 Applied Biosystems, Llc Methods, compositions, and kits comprising linker probes for quantifying polynucleotides
CA2476030A1 (en) * 2004-06-09 2005-12-09 Wilsun Xu A power signaling based technique for detecting islanding conditions in electric power distribution systems
US8124334B2 (en) * 2004-07-06 2012-02-28 Enzo Biochem, Inc. Selective detection of oncogenic HPV
WO2006034387A1 (en) * 2004-09-21 2006-03-30 Applera Corporation TWO-COLOR REAL-TIME/END-POINT QUANTITATION OF MICRORNAS (miRNAs)
EP1696040A1 (en) * 2005-02-28 2006-08-30 Eurofins Viralliance, Inc. Method of genotyping and phenotyping hepatitis B viruses resistant to antiviral molecules
EP1871896B1 (en) * 2005-04-12 2015-06-03 Olink AB Circle probes and their use in the identification of biomolecules
EP1871897B1 (en) 2005-04-12 2010-09-29 Olink AB Methods for production of oligonucleotides
NZ620811A (en) 2005-05-09 2015-09-25 Theranos Inc Point-of-care fluidic systems and uses thereof
US20070054301A1 (en) * 2005-09-06 2007-03-08 Gen-Probe Incorporated Methods, compositions and kits for isothermal amplification of nucleic acids
WO2007030759A2 (en) 2005-09-07 2007-03-15 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
EP3263117A1 (en) * 2005-11-28 2018-01-03 Imaginot Pty Ltd. Oral therapeutic compound delivery system
JP4580875B2 (ja) * 2006-01-20 2010-11-17 株式会社東芝 核酸検出法のためのプライマーの設計方法
US11287421B2 (en) 2006-03-24 2022-03-29 Labrador Diagnostics Llc Systems and methods of sample processing and fluid control in a fluidic system
US8007999B2 (en) 2006-05-10 2011-08-30 Theranos, Inc. Real-time detection of influenza virus
US7833716B2 (en) 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
US7501254B2 (en) 2006-07-20 2009-03-10 Ghc Technologies, Inc. Methods and compositions for amplification and capture of nucleic acid sequences
US8012744B2 (en) 2006-10-13 2011-09-06 Theranos, Inc. Reducing optical interference in a fluidic device
US20080113391A1 (en) 2006-11-14 2008-05-15 Ian Gibbons Detection and quantification of analytes in bodily fluids
AU2008206221A1 (en) * 2007-01-17 2008-07-24 Meridian Bioscience, Inc. Stable reagents and kits useful in loop-mediated isothermal amplification (LAMP)
US8158430B1 (en) 2007-08-06 2012-04-17 Theranos, Inc. Systems and methods of fluidic sample processing
MX352987B (es) 2007-10-02 2017-12-15 Theranos Ip Co Llc Dispositivos modulares de punto de cuidado y usos de los mismos.
US8420323B2 (en) * 2007-11-14 2013-04-16 Fujifilm Corporation Nucleic acid amplification method
US20090162845A1 (en) 2007-12-20 2009-06-25 Elazar Rabbani Affinity tag nucleic acid and protein compositions, and processes for using same
US8034568B2 (en) 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
US20090215050A1 (en) * 2008-02-22 2009-08-27 Robert Delmar Jenison Systems and methods for point-of-care amplification and detection of polynucleotides
WO2009117698A2 (en) 2008-03-21 2009-09-24 Nugen Technologies, Inc. Methods of rna amplification in the presence of dna
GB0820927D0 (en) 2008-11-14 2008-12-24 Isis Innovation Method
AU2010209528B2 (en) 2009-01-30 2015-10-01 Oxford Nanopore Technologies Limited Adaptors for nucleic acid constructs in transmembrane sequencing
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
US9393564B2 (en) 2009-03-30 2016-07-19 Ibis Biosciences, Inc. Bioagent detection systems, devices, and methods
EP2272980A1 (en) * 2009-07-09 2011-01-12 Bioesplora SRL Molecular probes for target nucleic acid detection and quantification
US9416409B2 (en) 2009-07-31 2016-08-16 Ibis Biosciences, Inc. Capture primers and capture sequence linked solid supports for molecular diagnostic tests
MY165876A (en) 2009-10-19 2018-05-18 Theranos Inc Integrated health data capture and analysis system
AU2010321576A1 (en) 2009-11-23 2012-06-07 Swift Biosciences, Inc. Devices to extend single stranded target molecules
CA2825196C (en) 2011-01-21 2021-01-05 Theranos, Inc. Systems and methods for sample use maximization
WO2013014451A1 (en) 2011-07-25 2013-01-31 Oxford Nanopore Technologies Limited Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores
US9109226B2 (en) * 2011-09-01 2015-08-18 New England Biolabs, Inc. Synthetic nucleic acids for polymerization reactions
US9416153B2 (en) 2011-10-11 2016-08-16 Enzo Life Sciences, Inc. Fluorescent dyes
US9970061B2 (en) 2011-12-27 2018-05-15 Ibis Biosciences, Inc. Bioagent detection oligonucleotides
EP2798089B1 (en) 2011-12-30 2018-05-23 Bio-rad Laboratories, Inc. Methods and compositions for performing nucleic acid amplification reactions
EP2802666B1 (en) 2012-01-13 2018-09-19 Data2Bio Genotyping by next-generation sequencing
WO2013153359A1 (en) 2012-04-10 2013-10-17 Oxford Nanopore Technologies Limited Mutant lysenin pores
EP2875154B1 (en) 2012-07-19 2017-08-23 Oxford Nanopore Technologies Limited SSB method for characterising a nucleic acid
EP3511424B1 (en) 2012-08-03 2023-10-18 University Of Washington Through Its Center For Commercialization Compositions and methods for improving nanopore sequencing
WO2014110127A1 (en) 2013-01-08 2014-07-17 Enzo Biochem, Inc. Diagnosis and treatment of viral diseases
US9617607B2 (en) 2013-01-08 2017-04-11 Enzo Biochem, Inc. Diagnosis and treatment of viral diseases
KR102168813B1 (ko) 2013-03-08 2020-10-22 옥스포드 나노포어 테크놀로지즈 리미티드 효소 정지 방법
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
CA2903874C (en) 2013-03-15 2024-02-20 Theranos, Inc. Nucleic acid amplification
SG11201507272RA (en) 2013-03-15 2015-10-29 Theranos Inc Nuclei acid amplification
EP3587592A1 (en) 2013-03-15 2020-01-01 Theranos IP Company, LLC Nucleic acid amplification
US10450595B2 (en) 2013-03-15 2019-10-22 Theranos Ip Company, Llc Nucleic acid amplification
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
BR112016004994A2 (pt) 2013-09-06 2021-05-11 Theranos Ip Company, Llc Sistemas e métodos para detectar doenças infecciosas
CN106103741B (zh) 2014-01-22 2020-03-13 牛津纳米孔技术公司 将一个或多个多核苷酸结合蛋白连接到靶多核苷酸的方法
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
WO2015166276A1 (en) 2014-05-02 2015-11-05 Oxford Nanopore Technologies Limited Method of improving the movement of a target polynucleotide with respect to a transmembrane pore
US10093918B2 (en) 2014-06-04 2018-10-09 Lucigen Corporation Sample collection and analysis devices
US10364458B2 (en) 2014-07-16 2019-07-30 Tangen Biosciences, Inc. Isothermal methods for amplifying nucleic acid samples
EP4053150A1 (en) 2014-09-01 2022-09-07 Vib Vzw Mutant csgg pores
CA2961209A1 (en) * 2014-09-17 2016-03-24 Theranos, Inc. Diagnostic methods and compositions
US10266885B2 (en) 2014-10-07 2019-04-23 Oxford Nanopore Technologies Ltd. Mutant pores
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
CN107208012B (zh) 2014-11-03 2021-06-29 唐恩生物科学公司 用于细胞、孢子或病毒捕获和破碎的装置和方法
GB201502809D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Mutant pore
GB201502810D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
EP3283887B1 (en) 2015-04-14 2021-07-21 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
CN109072295B (zh) 2015-12-08 2022-05-31 鲁汶大学研究与发展中心 修饰的纳米孔,包含其的组合物及其应用
US11766435B2 (en) * 2016-02-18 2023-09-26 Merck Sharp & Dohme Llc N3-substituted iminopyrimidinones as antimalarial agents
EP3423486B1 (en) 2016-03-02 2021-12-29 Oxford Nanopore Technologies PLC Mutant pore
CA3020203A1 (en) 2016-04-06 2017-10-12 Oxford Nanopore Technologies Limited Mutant pore
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
EP4202439B1 (en) 2016-06-09 2025-02-19 The Regents of the University of California Method of purifying and amplifying a nucleic acid
US10822648B1 (en) 2016-07-29 2020-11-03 Labrador Diagnostics Llc Hybrid multi-step nucleic acid amplification
IL267836B2 (en) 2017-01-04 2023-09-01 Complete Genomics Inc Stepwise sequence determination by unlabeled reversible terminations or natural nucleotides
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
WO2018236918A1 (en) 2017-06-20 2018-12-27 Bio-Rad Laboratories, Inc. MDA USING A BALL OLIGONUCLEOTIDE
CN117106037B (zh) 2017-06-30 2025-07-25 弗拉芒区生物技术研究所 新颖蛋白孔
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method

Family Cites Families (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE265429C (OSRAM) 1911-12-15 1913-10-10
US4711955A (en) * 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
US5260433A (en) 1982-06-23 1993-11-09 Enzo Diagnostics, Inc. Saccharide specific binding system labeled nucleotides
US5241060A (en) 1982-06-23 1993-08-31 Enzo Diagnostics, Inc. Base moiety-labeled detectable nucleatide
US5171534A (en) 1984-01-16 1992-12-15 California Institute Of Technology Automated DNA sequencing technique
US4707440A (en) 1984-01-30 1987-11-17 Enzo Biochem, Inc. Nucleic acid hybridization assay and detectable molecules useful in such assay
US4729947A (en) 1984-03-29 1988-03-08 The Board Of Regents Of The University Of Nebraska DNA sequencing
US5230781A (en) 1984-03-29 1993-07-27 Li-Cor, Inc. Sequencing near infrared and infrared fluorescence labeled DNA for detecting using laser diodes
US5346603A (en) 1984-03-29 1994-09-13 The Board Of Regents Of The University Of Nebraska DNA sequencing
US5360523A (en) 1984-03-29 1994-11-01 Li-Cor, Inc. DNA sequencing
US4749647A (en) * 1984-06-22 1988-06-07 Genetic Systems Corporation Polymerization-induced separation assay using recognition pairs
GB8421967D0 (en) * 1984-08-30 1984-10-03 Hickory Springs Mfg Co Polyurethane foams
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5093232A (en) * 1985-12-11 1992-03-03 Chiron Corporation Nucleic acid probes
JP3293820B2 (ja) 1985-12-13 2002-06-17 エンゾ− バイオケム インコ−ポレイテツド 標的ポリヌクレオチドにハイブリツド形成するための新規な一工程方法とポリヌクレオチド化合物
CA1340806C (en) 1986-07-02 1999-11-02 James Merrill Prober Method, system and reagents for dna sequencing
US5047519A (en) 1986-07-02 1991-09-10 E. I. Du Pont De Nemours And Company Alkynylamino-nucleotides
US5032570A (en) 1987-08-04 1991-07-16 Hitachi Metals, Ltd. Method for producing ceramic superconducting material using intermediate products
DD265429A1 (de) * 1987-09-30 1989-03-01 Adl Inst Phytopathologie Verfahren zur nichtradioaktiven markierung von polynukleotiden
CA1323293C (en) 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
US5260428A (en) * 1988-07-11 1993-11-09 Boehringer Mannheim Gmbh Agent for the detection of substances with hydrolase activity
US5508178A (en) 1989-01-19 1996-04-16 Rose; Samuel Nucleic acid amplification using single primer
US5503975A (en) * 1989-03-01 1996-04-02 City Of Hope Mechanism based inhibitors of DNA methyltransferase
JPH0750041Y2 (ja) 1990-01-17 1995-11-15 大同特殊鋼株式会社 連続鋳造設備におけるタンディッシュ交換装置
WO1992000989A1 (en) 1990-07-10 1992-01-23 Imperial Chemical Industries Plc Non-isotopic nucleic acid labelling method
US5595891A (en) 1990-07-19 1997-01-21 Behringwerke Ag Method for producing a polynucleotide for use in single primer amplification
US5439793A (en) 1990-07-19 1995-08-08 Syntex (U.S.A.) Inc. Method for producing a polynucleotide having an intramolecularly base-paired structure
US5518900A (en) * 1993-01-15 1996-05-21 Molecular Tool, Inc. Method for generating single-stranded DNA molecules
US5569582A (en) * 1991-07-15 1996-10-29 Institute Of Molecular Biology & Technology Rapid amplification and detection of nucleic acids
US5714323A (en) * 1991-08-30 1998-02-03 The University Of Medecine And Dentistry Of New Jersey Over expression of single-stranded molecules
US5474796A (en) 1991-09-04 1995-12-12 Protogene Laboratories, Inc. Method and apparatus for conducting an array of chemical reactions on a support surface
US5612199A (en) 1991-10-11 1997-03-18 Behringwerke Ag Method for producing a polynucleotide for use in single primer amplification
EP0656065A1 (en) 1991-10-31 1995-06-07 University Of Pittsburgh Reverse dot blot hybridization using tandem head-to-tail monomers containing probes synthesized by staggered complementary primers
US5270184A (en) 1991-11-19 1993-12-14 Becton, Dickinson And Company Nucleic acid target generation
ZA929351B (en) * 1991-12-11 1993-06-04 Igen Inc Electrochemiluminescent label for DNA assays.
FR2687679B1 (fr) * 1992-02-05 1994-10-28 Centre Nat Rech Scient Oligothionucleotides.
US5462854A (en) 1993-04-19 1995-10-31 Beckman Instruments, Inc. Inverse linkage oligonucleotides for chemical and enzymatic processes
US5470723A (en) 1993-05-05 1995-11-28 Becton, Dickinson And Company Detection of mycobacteria by multiplex nucleic acid amplification
WO1995002690A1 (en) 1993-07-13 1995-01-26 Abbott Laboratories Nucleotide sequences and process for amplifying and detection of hepatitis b virus
CA2105364A1 (en) * 1993-09-01 1995-03-02 Eric T. Kool Stem-loop oligonucleotides containing parallel and antiparallel binding domains
DE4331670A1 (de) 1993-09-17 1995-03-23 Hoechst Ag Neue Antisense-Oligonucleotide gegen HSV-1 sowie deren Herstellung
US5470184A (en) * 1994-01-14 1995-11-28 Chandler; Charles W. Screw hole plug
US5462584A (en) 1994-03-21 1995-10-31 Gas Research Institute Process for dehydrating natural gas
FR2721945B1 (fr) 1994-07-04 1996-10-18 David Fabrice Accroissement genique, un procede d'amplicication genique isotherme et ses applications
FR2726277B1 (fr) 1994-10-28 1996-12-27 Bio Merieux Oligonucleotide utilisable comme amorce dans une methode d'amplification basee sur une replication avec deplacement de brin
US5665545A (en) * 1994-11-28 1997-09-09 Akzo Nobel N.V. Terminal repeat amplification method
US5798276A (en) * 1995-06-07 1998-08-25 Molecular Probes, Inc. Reactive derivatives of sulforhodamine 101 with enhanced hydrolytic stability
AT402203B (de) 1995-06-13 1997-03-25 Himmler Gottfried Dipl Ing Dr Verfahren zur transkriptionsfreien amplifizierung von nucleinsäuren
WO1997004131A1 (en) 1995-07-21 1997-02-06 Forsyth Dental Infirmary For Children Single primer amplification of polynucleotide hairpins
US6114150A (en) * 1995-11-29 2000-09-05 Yale University Amplification of nucleic acids
GB9609441D0 (en) 1996-05-04 1996-07-10 Zeneca Ltd Process
US5821356A (en) 1996-08-12 1998-10-13 The Perkin Elmer Corporation Propargylethoxyamino nucleotides
JPH10312826A (ja) 1997-03-10 1998-11-24 Sanyo Electric Co Ltd 非水電解質電池及びその充電方法
EP0973788A1 (en) 1997-04-02 2000-01-26 Amersham Pharmacia Biotech UK Limited Tricyclic base analogues
GB9717061D0 (en) 1997-08-13 1997-10-15 Tepnel Medical Ltd Amplification of nucleic acids
US6087099A (en) * 1997-09-08 2000-07-11 Myriad Genetics, Inc. Method for sequencing both strands of a double stranded DNA in a single sequencing reaction
US6124120A (en) 1997-10-08 2000-09-26 Yale University Multiple displacement amplification
GB9810865D0 (en) 1998-05-20 1998-07-22 Zeneca Ltd Nucleic acid sequence identification
US5991238A (en) 1998-06-09 1999-11-23 Western Atlas International, Inc. Weighted backus filter method of combining dual sensor traces
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
US6743605B1 (en) * 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
WO2000028082A1 (fr) * 1998-11-09 2000-05-18 Eiken Kagaku Kabushiki Kaisha Procede de synthese d'acide nucleique
US6114123A (en) 1999-06-14 2000-09-05 Incyte Pharmaceuticals, Inc. Lipocalin family protein
DE60025840T2 (de) 1999-11-08 2006-07-27 Eiken Kagaku K.K. Methode zum nachweis von variationen oder polymorphismen
BR0015382B1 (pt) 1999-11-08 2014-04-29 Eiken Chemical Processos e kits para sintetizacao e amplificação do acido nucleico bem como, processos para detectar uma sequencia de nucleotideo alvo e de uma mutacao da dita sequencia

Also Published As

Publication number Publication date
US20030165939A1 (en) 2003-09-04
EP0971039A2 (en) 2000-01-12
US8133989B2 (en) 2012-03-13
US20030165938A1 (en) 2003-09-04
US20030170682A1 (en) 2003-09-11
EP0971039B1 (en) 2011-03-23
CA2273064A1 (en) 1999-12-24
IL130477A (en) 2004-03-28
JP4675141B2 (ja) 2011-04-20
JP2000037194A (ja) 2000-02-08
US8288092B2 (en) 2012-10-16
JP2005270108A (ja) 2005-10-06
JP4493330B2 (ja) 2010-06-30
US20030170681A1 (en) 2003-09-11
JP2004141170A (ja) 2004-05-20
US20090263789A1 (en) 2009-10-22
EP0971039A3 (en) 2004-01-28
US6764821B1 (en) 2004-07-20
US7468245B2 (en) 2008-12-23
US20090286285A1 (en) 2009-11-19
US20030165936A1 (en) 2003-09-04
US6743605B1 (en) 2004-06-01
DE69943292D1 (de) 2011-05-05
US20030104460A1 (en) 2003-06-05
US8486633B2 (en) 2013-07-16
US7264930B2 (en) 2007-09-04
US7713691B2 (en) 2010-05-11
US20050260573A1 (en) 2005-11-24
US7485417B2 (en) 2009-02-03
IL130477A0 (en) 2000-06-01

Similar Documents

Publication Publication Date Title
CA2273064C (en) Novel processes for amplifying nucleic acid, post-termination labeling process for nucleic acid sequencing and producing nucleic acid having decreased thermodynamic stability
US5942609A (en) Ligation assembly and detection of polynucleotides on solid-support
AU764099B2 (en) Polynucleotide sequencing method
JP3867926B2 (ja) 核酸の増幅法
EP2049682A2 (en) Method of library preparation avoiding the formation of adaptor dimers
JP2003507024A (ja) Pna−dnaキメラの3’末端でのポリメラーゼ伸長
EP1590482B1 (en) Nucleic acid amplification using non-standard bases
US8445664B2 (en) Kits for amplifying and detecting nucleic acid sequences
CA2475018C (en) Novel processes for amplifying nucleic acid, post-termination labeling process for nucleic acid sequencing and producing nucleic acid having decreased thermodynamic stability
IL152261A (en) Process for non-linearly amplifying a nucleic acid sequence
WO2005073409A2 (en) Methods, compositions, and kits for amplifying and sequencing polynucleotides
JPH05211873A (ja) 核酸鋳型の特異的増殖方法および核酸の決定方法

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20190617