CA2250712C - Chimeric isoprenoid synthases and uses thereof - Google Patents

Chimeric isoprenoid synthases and uses thereof Download PDF

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Publication number
CA2250712C
CA2250712C CA002250712A CA2250712A CA2250712C CA 2250712 C CA2250712 C CA 2250712C CA 002250712 A CA002250712 A CA 002250712A CA 2250712 A CA2250712 A CA 2250712A CA 2250712 C CA2250712 C CA 2250712C
Authority
CA
Canada
Prior art keywords
chimeric
synthase
isoprenoid
isoprenoid synthase
domain
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA002250712A
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English (en)
French (fr)
Other versions
CA2250712A1 (en
Inventor
Joseph Chappell
Kyoungwhan Back
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Kentucky
Original Assignee
University of Kentucky
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Filing date
Publication date
Application filed by University of Kentucky filed Critical University of Kentucky
Publication of CA2250712A1 publication Critical patent/CA2250712A1/en
Application granted granted Critical
Publication of CA2250712C publication Critical patent/CA2250712C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04Antibacterial agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/10Antimycotics
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00Antineoplastic agents
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88Lyases (4.)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Epidemiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
CA002250712A 1996-04-12 1997-04-11 Chimeric isoprenoid synthases and uses thereof Expired - Lifetime CA2250712C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/631,341 1996-04-12
US08/631,341 US5824774A (en) 1996-04-12 1996-04-12 Chimeric isoprenoid synthases and uses thereof
PCT/US1997/005986 WO1997038703A1 (en) 1996-04-12 1997-04-11 Chimeric isoprenoid synthases and uses thereof

Publications (2)

Publication Number Publication Date
CA2250712A1 CA2250712A1 (en) 1997-10-23
CA2250712C true CA2250712C (en) 2008-07-08

Family

ID=24530791

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002250712A Expired - Lifetime CA2250712C (en) 1996-04-12 1997-04-11 Chimeric isoprenoid synthases and uses thereof

Country Status (25)

Country Link
US (2) US5824774A (OSRAM)
EP (2) EP0904095B1 (OSRAM)
JP (4) JP2000508899A (OSRAM)
KR (1) KR20000005385A (OSRAM)
AP (1) AP808A (OSRAM)
AR (1) AR006638A1 (OSRAM)
AT (1) ATE223225T1 (OSRAM)
AU (1) AU2726497A (OSRAM)
BR (1) BR9708650A (OSRAM)
CA (1) CA2250712C (OSRAM)
CZ (1) CZ294613B6 (OSRAM)
DE (2) DE904095T1 (OSRAM)
EA (1) EA001622B1 (OSRAM)
ES (1) ES2132046T3 (OSRAM)
GR (1) GR990300025T1 (OSRAM)
ID (1) ID19769A (OSRAM)
IL (2) IL126486A0 (OSRAM)
IN (1) IN186316B (OSRAM)
OA (1) OA10895A (OSRAM)
PL (1) PL329404A1 (OSRAM)
PT (1) PT904095E (OSRAM)
TR (1) TR199802043T2 (OSRAM)
TW (1) TW509724B (OSRAM)
WO (1) WO1997038703A1 (OSRAM)
ZA (1) ZA973108B (OSRAM)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7186891B1 (en) * 1996-04-12 2007-03-06 University Of Kentucky, Research Foundation Plant cells and plants expressing chimeric isoprenoid synthases
US8106260B2 (en) * 1996-04-12 2012-01-31 The Board Of Trustees Of The University Of Kentucky Chimeric isoprenoid synthases and uses thereof
US5824774A (en) * 1996-04-12 1998-10-20 Board Of Trustees Of The University Of Kentucky Chimeric isoprenoid synthases and uses thereof
US5945726A (en) * 1996-12-16 1999-08-31 Micron Technology, Inc. Lateral bipolar transistor
AR022383A1 (es) * 1998-09-18 2002-09-04 Univ Kentucky Res Found Sintasas
US6358712B1 (en) 1999-01-05 2002-03-19 Trustee Of Boston University Ordered gene assembly
CA2360011A1 (en) * 1999-01-05 2000-07-13 Trustees Of Boston University Ordered gene assembly
US20040005673A1 (en) * 2001-06-29 2004-01-08 Kevin Jarrell System for manipulating nucleic acids
US20030033626A1 (en) 2000-07-31 2003-02-13 Hahn Frederick M. Manipulation of genes of the mevalonate and isoprenoid pathways to create novel traits in transgenic organisms
AU2013204829B2 (en) * 2001-03-09 2013-07-04 University Of Kentucky Research Foundation Cytochrome P450s and uses thereof
ATE513046T1 (de) * 2001-03-09 2011-07-15 Univ Kentucky Res Found Cytochrom p450s und verwendungen davon
AU2012202780B2 (en) * 2001-03-09 2014-07-03 University Of Kentucky Research Foundation Cytochrome P450s and Uses Thereof
US7192751B2 (en) * 2001-12-06 2007-03-20 The Regents Of The University Of California Biosynthesis of amorpha-4,11-diene
US7172886B2 (en) * 2001-12-06 2007-02-06 The Regents Of The University Of California Biosynthesis of isopentenyl pyrophosphate
US7442785B2 (en) * 2003-07-24 2008-10-28 The University Of Kentucky Research Foundation Sesquiterpene synthase gene and protein
US8124811B2 (en) * 2007-03-20 2012-02-28 Allylix, Inc. Fragrance and methods for production of 5-epi-β-vetivone, 2-isopropyl-6,10-dimethyl-spiro[4.5]deca-2,6-dien-8-one, and 2-isopropyl-6,10-dimethyl-spiro[4.5]deca-1,6-dien-8-one
NZ581473A (en) 2007-06-01 2012-02-24 Scripps Research Inst Use of genetically modified organisms to generate biomass degrading enzymes
WO2009036087A1 (en) 2007-09-11 2009-03-19 Sapphire Energy, Inc. Methods of producing organic products with photosynthetic organisms and products and compositions thereof
MX2010002723A (es) * 2007-09-11 2010-05-21 Sapphire Energy Inc Produccion de moleculas mediante organismos fotosinteticos.
BRPI0823506A8 (pt) * 2007-12-13 2018-05-15 Danisco Us Inc Genencor Div composições e métodos para a produção de isoprene
US8034992B2 (en) * 2008-06-16 2011-10-11 Academia Sinica Gibberellin 2-oxidase genes and uses thereof
US8609371B2 (en) * 2008-08-12 2013-12-17 Allylix, Inc. Isoprenoid compounds
BR112013010244A2 (pt) 2010-10-29 2016-07-05 Allylix Inc polipeptídeos de valenceno sintase modificados que codificam moléculas de ácido nucleico e seus usos
US9550815B2 (en) 2012-01-23 2017-01-24 University Of British Columbia ABC terpenoid transporters and methods of using the same
JP5950253B2 (ja) * 2012-02-13 2016-07-13 住友ゴム工業株式会社 イソプレノイドの製造方法、及びイソプレノイド
WO2014022434A1 (en) 2012-07-30 2014-02-06 Allylix, Inc. Sclareol and labdenediol diphosphate synthase polypeptides encoding nucleic acid molecules and uses thereof
CA2889945A1 (en) 2012-11-01 2014-05-08 The University Of British Columbia Cytochrome p450 and cytochrome p450 reductase polypeptides, encoding nucleic acid molecules and uses thereof
US10000749B2 (en) 2013-03-14 2018-06-19 Evolva, Inc. Valencene synthase polypeptides, encoding nucleic acid molecules and uses thereof
CN111621508B (zh) * 2020-06-11 2022-07-01 云南中烟工业有限责任公司 烟草萜类合成酶NtTPS7基因及其载体与应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2159735A1 (en) * 1993-04-02 1994-10-13 Terry Euclaire Meyer Southern corn rootworm control with limonene
CA2118071C (en) * 1993-10-28 2004-09-14 Rodney B. Croteau Dna encoding limonene synthase from mentha spicata
US5824774A (en) * 1996-04-12 1998-10-20 Board Of Trustees Of The University Of Kentucky Chimeric isoprenoid synthases and uses thereof

Also Published As

Publication number Publication date
EP1229122A2 (en) 2002-08-07
ATE223225T1 (de) 2002-09-15
ZA973108B (en) 1997-11-04
DE904095T1 (de) 1999-10-07
ID19769A (id) 1998-07-30
AP9700971A0 (en) 1997-07-31
JP2015171387A (ja) 2015-10-01
ES2132046T3 (es) 2003-03-16
EP1229122A3 (en) 2002-10-09
WO1997038703A1 (en) 1997-10-23
BR9708650A (pt) 1999-08-03
IN186316B (OSRAM) 2001-08-04
TR199802043T2 (xx) 1999-01-18
GR990300025T1 (en) 1999-07-30
US5824774A (en) 1998-10-20
CA2250712A1 (en) 1997-10-23
DE69715192D1 (de) 2002-10-10
HK1017275A1 (en) 1999-11-19
AR006638A1 (es) 1999-09-08
JP2015057074A (ja) 2015-03-26
AU2726497A (en) 1997-11-07
ES2132046T1 (es) 1999-08-16
CZ317998A3 (cs) 1999-02-17
DE69715192T2 (de) 2003-04-30
PT904095E (pt) 2002-12-31
IL126486A (en) 2006-04-10
CZ294613B6 (cs) 2005-02-16
EP0904095A4 (en) 1999-07-07
AP808A (en) 2000-02-29
OA10895A (en) 2001-10-11
TW509724B (en) 2002-11-11
EP0904095A1 (en) 1999-03-31
IL126486A0 (en) 1999-08-17
JP2012005499A (ja) 2012-01-12
EP0904095B1 (en) 2002-09-04
PL329404A1 (en) 1999-03-29
US6072045A (en) 2000-06-06
JP2000508899A (ja) 2000-07-18
KR20000005385A (ko) 2000-01-25
EA001622B1 (ru) 2001-06-25
EA199800917A1 (ru) 1999-04-29

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Effective date: 20170411