CA2250084A1 - Use of arabinogalactan in cell cryopreservation media - Google Patents
Use of arabinogalactan in cell cryopreservation media Download PDFInfo
- Publication number
- CA2250084A1 CA2250084A1 CA002250084A CA2250084A CA2250084A1 CA 2250084 A1 CA2250084 A1 CA 2250084A1 CA 002250084 A CA002250084 A CA 002250084A CA 2250084 A CA2250084 A CA 2250084A CA 2250084 A1 CA2250084 A1 CA 2250084A1
- Authority
- CA
- Canada
- Prior art keywords
- medium
- cells
- cryoprotective
- arabinogalactan
- concentration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- SATHPVQTSSUFFW-UHFFFAOYSA-N 4-[6-[(3,5-dihydroxy-4-methoxyoxan-2-yl)oxymethyl]-3,5-dihydroxy-4-methoxyoxan-2-yl]oxy-2-(hydroxymethyl)-6-methyloxane-3,5-diol Chemical compound OC1C(OC)C(O)COC1OCC1C(O)C(OC)C(O)C(OC2C(C(CO)OC(C)C2O)O)O1 SATHPVQTSSUFFW-UHFFFAOYSA-N 0.000 title claims abstract description 47
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/12—Chemical aspects of preservation
- A01N1/122—Preservation or perfusion media
- A01N1/125—Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US621,489 | 1996-03-25 | ||
| US08/621,489 US5897987A (en) | 1996-03-25 | 1996-03-25 | Use of arabinogalactan in cell cryopreservation media |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2250084A1 true CA2250084A1 (en) | 1997-10-02 |
Family
ID=24490374
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002250084A Abandoned CA2250084A1 (en) | 1996-03-25 | 1997-03-25 | Use of arabinogalactan in cell cryopreservation media |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US5897987A (enExample) |
| EP (1) | EP0891134B1 (enExample) |
| JP (1) | JP4031044B2 (enExample) |
| CA (1) | CA2250084A1 (enExample) |
| DE (1) | DE69713312T2 (enExample) |
| WO (1) | WO1997035472A1 (enExample) |
Families Citing this family (37)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6378527B1 (en) * | 1998-04-08 | 2002-04-30 | Chondros, Inc. | Cell-culture and polymer constructs |
| CA2332986C (en) | 1998-05-26 | 2009-09-08 | Lifecell Corporation | Cryopreservation of human red blood cells |
| DE19835368C1 (de) * | 1998-08-05 | 2000-02-10 | Juergen Steinmeyer | Verfahren zur Herstellung einer Knorpelzellsuspension |
| GR1004204B (el) * | 1999-09-29 | 2003-09-05 | Κρυοσυντηρημενα αμνιακα κυτταρα ανθρωπου για μελλοντικες θεραπευτικες, γενετικες, διαγνωστικες και αλλες χρησεις | |
| US7112576B1 (en) | 1999-12-10 | 2006-09-26 | Regents Of The University Of Minnesota | Compositions and methods for cryopreservation of peripheral blood lymphocytes |
| US7674576B2 (en) * | 2001-01-12 | 2010-03-09 | Abs Corporation | Semen extender composition and methods for manufacturing and using |
| EP1475434A1 (en) * | 2003-05-09 | 2004-11-10 | Oncoscience AG | Method for storing tumor cells |
| ATE494777T1 (de) * | 2003-10-09 | 2011-01-15 | Core Dynamics Ltd | Verfahren zum tiefgefrieren, auftauen und transplantieren von lebensfähigem knorpel |
| US8196416B2 (en) * | 2004-02-02 | 2012-06-12 | Core Dynamics Limited | Device for directional cooling of biological matter |
| EP1711053A2 (en) * | 2004-02-02 | 2006-10-18 | I.M.T. Interface Multigrad Technology Ltd. | Biological material and methods and solutions for preservation thereof |
| DE602005019998D1 (de) * | 2004-06-07 | 2010-04-29 | Core Dynamics Ltd | Verfahren zur sterilisation von biologischen präparaten |
| WO2006016372A1 (en) * | 2004-08-12 | 2006-02-16 | I.M.T. Interface Multigrad Technology Ltd. | Method and apparatus for freezing or thawing of a biological material |
| US20080160496A1 (en) * | 2005-02-22 | 2008-07-03 | Victor Rzepakovsky | Preserved Viable Cartilage, Method for Its Preservation, and System and Devices Used Therefor |
| US20100233295A1 (en) * | 2005-03-02 | 2010-09-16 | Vibha Gupta | Aqueous moisturizers and lubricants and uses thereof |
| US8703198B2 (en) | 2005-03-02 | 2014-04-22 | Aquatrove Biosciences | Water-based personal moisturizers and lubricants, in particular vaginal lubricants, and uses thereof |
| EP2361591A3 (en) * | 2005-06-21 | 2011-12-21 | Pervasis Therapeutics, Inc. | Methods and compositions for enhancing vascular access |
| US8198085B2 (en) * | 2005-08-03 | 2012-06-12 | Core Dynamics Limited | Somatic cells for use in cell therapy |
| US20070110666A1 (en) * | 2005-09-30 | 2007-05-17 | Paul Pevsner | Methods for preparation of live body tissues for examination |
| US20070098695A1 (en) * | 2005-10-31 | 2007-05-03 | Deolden James | Methods of islet separation during isolation |
| AU2006329195B2 (en) * | 2005-12-22 | 2012-09-20 | John E. Davies | Viable cells from frozen umbilical cord tissue |
| US20070259327A1 (en) * | 2006-05-03 | 2007-11-08 | Bioverde. Inc. | Cryopreservation medium and cryopreservation method for tissues and cells |
| JP2008099659A (ja) * | 2006-09-22 | 2008-05-01 | Ina Food Ind Co Ltd | 微生物の環境ストレス保護剤、並びに発酵食品及びその製造方法 |
| US20080113431A1 (en) * | 2006-11-15 | 2008-05-15 | Mariposa Biotechnology, Inc. | Methods and compositions for cryopreserving oocytes |
| US20080113432A1 (en) * | 2006-11-15 | 2008-05-15 | Mariposa Biotechnology, Inc. | Methods and compositions for reanimating cryopreserved oocytes |
| US20090123436A1 (en) * | 2007-11-08 | 2009-05-14 | Surmodics, Inc. | Cryopreservative compositions and methods |
| US20090202978A1 (en) * | 2008-02-13 | 2009-08-13 | Ginadi Shaham | Method and apparatus for freezing of a biological material |
| US9186253B2 (en) | 2013-02-22 | 2015-11-17 | Allosource | Cartilage mosaic compositions and methods |
| EP2967874B1 (en) | 2013-03-15 | 2019-11-20 | AlloSource | Perforated osteochondral allograft compositions |
| EP2970882B1 (en) | 2013-03-15 | 2018-11-28 | AlloSource | Cell repopulated collagen matrix for soft tissue repair and regeneration |
| ES2529265B1 (es) * | 2013-07-18 | 2015-10-07 | Universidad De Sevilla | Monitorización mediante TAC de procesos de preservación en frío y criopreservación de material biológico |
| JP6373241B2 (ja) * | 2015-10-05 | 2018-08-15 | 有限会社ジーエヌコーポレーション | 細胞の保存方法 |
| US10004697B2 (en) | 2016-11-16 | 2018-06-26 | M Pharmaceutical Usa Inc. | In vivo sperm selection for treating male infertility |
| GB201708999D0 (en) | 2017-06-06 | 2017-07-19 | Univ Warwick | Recrystallization Inhibitor |
| WO2019156601A1 (en) * | 2018-02-08 | 2019-08-15 | Al Otaibi Noha Abdullah S | High efficiency cryo-additive agent to improve mammalian cells biopreservation |
| CN111034712A (zh) * | 2019-12-23 | 2020-04-21 | 成都大熊猫繁育研究基地 | 一种大熊猫乳汁保存方法 |
| GB202007905D0 (en) * | 2020-05-27 | 2020-07-08 | Univ Edinburgh | Cryopressing macrophages |
| CN113615681A (zh) * | 2021-08-27 | 2021-11-09 | 郑州源创吉因实业有限公司 | 一种免疫细胞的冻存液及冻存方法 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4327177A (en) * | 1969-04-10 | 1982-04-27 | Wallace Shrimpton | Method and means for controlling the sex of mammalian offspring and product therefor |
| US4007087A (en) * | 1975-10-17 | 1977-02-08 | Gametrics Limited | Sperm fractionation and storage |
| US4004975A (en) * | 1975-12-30 | 1977-01-25 | The United States Of America As Represented By The Secretary Of The Navy | Method of isolating and cryopreserving human white cells from whole blood |
| US4605558A (en) * | 1984-04-20 | 1986-08-12 | Wallace Shrimpton | Process for cell separation |
| US5490991A (en) * | 1986-07-03 | 1996-02-13 | Advanced Magnetics, Inc. | Directed delivery of radioprotectants using a receptor specific carrier |
| US5478576A (en) * | 1986-07-03 | 1995-12-26 | Advanced Magnetics, Inc. | Arabinogalactan derivatives and uses thereof |
| US5071741A (en) * | 1988-04-18 | 1991-12-10 | Cryolife, Inc. | Cryoprotective agent and its use in cryopreservation of cellular matter |
| WO1992008347A1 (en) * | 1988-04-18 | 1992-05-29 | Cryolife, Inc. | Cryoprotective agent |
| US5116969B1 (en) * | 1990-04-26 | 1997-04-01 | Larex International Inc | Ultrarefined arabinogalactan product |
| GB9009674D0 (en) * | 1990-04-30 | 1990-06-20 | Zaadunie Bv | Improvements in or relating to organic compounds |
| EP0646018A1 (en) * | 1992-06-17 | 1995-04-05 | Advanced Magnetics Incorporated | Arabinogalactan derivatives and uses thereof |
| US5405742A (en) * | 1993-07-16 | 1995-04-11 | Cyromedical Sciences, Inc. | Solutions for tissue preservation and bloodless surgery and methods using same |
| JPH07113001A (ja) * | 1993-08-23 | 1995-05-02 | Tsumura & Co | 新規多糖体及び該多糖体を有効成分とする放射線防護剤 |
-
1996
- 1996-03-25 US US08/621,489 patent/US5897987A/en not_active Expired - Lifetime
-
1997
- 1997-03-25 CA CA002250084A patent/CA2250084A1/en not_active Abandoned
- 1997-03-25 DE DE69713312T patent/DE69713312T2/de not_active Expired - Fee Related
- 1997-03-25 JP JP53454397A patent/JP4031044B2/ja not_active Expired - Fee Related
- 1997-03-25 WO PCT/US1997/004764 patent/WO1997035472A1/en not_active Ceased
- 1997-03-25 EP EP97916960A patent/EP0891134B1/en not_active Expired - Lifetime
-
1998
- 1998-08-21 US US09/138,101 patent/US6593138B1/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| WO1997035472A1 (en) | 1997-10-02 |
| JP2000507547A (ja) | 2000-06-20 |
| US6593138B1 (en) | 2003-07-15 |
| DE69713312T2 (de) | 2003-02-13 |
| EP0891134A1 (en) | 1999-01-20 |
| EP0891134B1 (en) | 2002-06-12 |
| US5897987A (en) | 1999-04-27 |
| JP4031044B2 (ja) | 2008-01-09 |
| DE69713312D1 (de) | 2002-07-18 |
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