CA2214029A1 - Formulations en ampoule unidose de complexes adn/lipides - Google Patents
Formulations en ampoule unidose de complexes adn/lipides Download PDFInfo
- Publication number
- CA2214029A1 CA2214029A1 CA002214029A CA2214029A CA2214029A1 CA 2214029 A1 CA2214029 A1 CA 2214029A1 CA 002214029 A CA002214029 A CA 002214029A CA 2214029 A CA2214029 A CA 2214029A CA 2214029 A1 CA2214029 A1 CA 2214029A1
- Authority
- CA
- Canada
- Prior art keywords
- solution
- cationic lipid
- plasmid dna
- lipid
- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7088—Compounds having three or more nucleosides or nucleotides
- A61K31/711—Natural deoxyribonucleic acids, i.e. containing only 2'-deoxyriboses attached to adenine, guanine, cytosine or thymine and having 3'-5' phosphodiester links
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/177—Receptors; Cell surface antigens; Cell surface determinants
- A61K38/1774—Immunoglobulin superfamily (e.g. CD2, CD4, CD8, ICAM molecules, B7 molecules, Fc-receptors, MHC-molecules)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/19—Cytokines; Lymphokines; Interferons
- A61K38/20—Interleukins [IL]
- A61K38/2013—IL-2
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/54—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
- A61K47/543—Lipids, e.g. triglycerides; Polyamines, e.g. spermine or spermidine
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/10—Dispersions; Emulsions
- A61K9/127—Liposomes
- A61K9/1271—Non-conventional liposomes, e.g. PEGylated liposomes, liposomes coated with polymers
- A61K9/1272—Non-conventional liposomes, e.g. PEGylated liposomes, liposomes coated with polymers with substantial amounts of non-phosphatidyl, i.e. non-acylglycerophosphate, surfactants as bilayer-forming substances, e.g. cationic lipids
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Epidemiology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Dispersion Chemistry (AREA)
- Medicinal Preparation (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
L'invention concerne des formulations en ampoule unidose de complexes ADN plasmidique/lipides cationiques, lesquelles sont destinées à une utilisation clinique chez l'homme, et préparées selon plusieurs procédés différents, par exemple, selon un procédé comprenant les étapes consistant: (a) à stériliser en autoclave une solution de lipides cationiques, à un niveau de concentration suffisamment élevé pour empêcher principalement la dégradation des lipides au cours de cette stérilisation; (b) à diluer la solution de lipides cationiques stérilisée de l'étape (a), à un degré suffisant pour empêcher notamment l'agrégation des lipides lors de l'étape (d) ci-après; (c) à stériliser par filtrage une solution d'ADN plasmidique; (d) à ajouter la solution d'ADN plasmidique stérilisée de l'étape (c) à la solution stérilisée et diluée de lipides cationiques de l'étape (b), à un niveau de force ionique inférieur à celui de l'isotonicité, afin de former des complexes ADN/lipides; et (e) à ajuster les complexes ADN/lipides de l'étape (d) afin qu'ils soient proches de l'isotonicité.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US42846395A | 1995-04-25 | 1995-04-25 | |
US08/428,463 | 1995-04-25 |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2214029A1 true CA2214029A1 (fr) | 1996-10-31 |
Family
ID=23699001
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002214029A Abandoned CA2214029A1 (fr) | 1995-04-25 | 1996-04-11 | Formulations en ampoule unidose de complexes adn/lipides |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP0826063A1 (fr) |
JP (1) | JPH11504631A (fr) |
CA (1) | CA2214029A1 (fr) |
WO (1) | WO1996034109A1 (fr) |
Families Citing this family (55)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5770715A (en) * | 1995-03-22 | 1998-06-23 | Toagosei Co., Ltd. | Hammerhead-like nucleic acid analogues and their synthesis |
AU729077B2 (en) * | 1996-08-26 | 2001-01-25 | Transgene S.A. | Cationic lipid-nucleic acid complexes |
WO1998019709A2 (fr) * | 1996-11-04 | 1998-05-14 | Qiagen Gmbh | Reactifs cationiques pour transfection |
FR2772047B1 (fr) | 1997-12-05 | 2004-04-09 | Ct Nat D Etudes Veterinaires E | Sequence genomique et polypeptides de circovirus associe a la maladie de l'amaigrissement du porcelet (map), applications au diagnostic et a la prevention et/ou au traitement de l'infection |
DE69931166T2 (de) * | 1998-08-14 | 2007-02-15 | Valentis Inc., Burlingame | Co-lyophilisierter komplex umfassend einen nukleinsäurevektor und ein formulierungsagens |
US6943152B1 (en) | 1999-06-10 | 2005-09-13 | Merial | DNA vaccine-PCV |
FR2794648B1 (fr) * | 1999-06-10 | 2003-03-07 | Merial Sas | Vaccins adn pour animaux de compagnie et de sport |
PL210451B1 (pl) * | 1999-06-10 | 2012-01-31 | Merial Sas | Szczepionka DNA przeciwko wirusowi nosówki psów (CDV) |
DE19944262A1 (de) * | 1999-09-15 | 2001-03-29 | Cardiogene Gentherapeutische S | Pharmazeutische Zusammensetzung in Form eines Nukleinsäure-Lipid-Komplexes, ihre Herstellung und Verwendung in der Gentherapie |
US6852705B2 (en) | 2000-01-21 | 2005-02-08 | Merial | DNA vaccines for farm animals, in particular bovines and porcines |
US7078388B2 (en) | 2000-01-21 | 2006-07-18 | Merial | DNA vaccines for farm animals, in particular bovines and porcines |
FR2804028B1 (fr) * | 2000-01-21 | 2004-06-04 | Merial Sas | Vaccins adn ameliores pour animaux de rente |
FR2810888B1 (fr) | 2000-06-29 | 2004-07-30 | Merial Sas | Vaccin contre la fievre aphteuse |
FR2823222B1 (fr) | 2001-04-06 | 2004-02-06 | Merial Sas | Vaccin contre le virus de la fievre du nil |
MXPA05003049A (es) | 2002-09-19 | 2005-11-17 | Us Gov Health & Human Serv | Polipeptidos de phlebotomus ariasi, polipeptidos de phlebotomus perniciosus y metodos de uso. |
EP1572968B1 (fr) | 2002-10-29 | 2009-04-22 | The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Polypeptides de lutzomyia longipalpis et methodes d'utilisation desdits polypeptides |
US7354593B2 (en) | 2002-12-09 | 2008-04-08 | Merial Limited | Coccidial vaccine and methods of making and using same |
US7468273B2 (en) | 2003-05-01 | 2008-12-23 | Meial Limited | Canine GHRH gene, polypeptides and methods of use |
RS51324B (sr) | 2005-04-25 | 2010-12-31 | Merial Ltd. | Vakcine protiv nipah virusa |
CN101365484A (zh) | 2005-08-15 | 2009-02-11 | 瓦克辛公司 | 通过给药非复制型载体化疫苗而进行的对禽类的免疫 |
US20080241184A1 (en) | 2005-08-25 | 2008-10-02 | Jules Maarten Minke | Canine influenza vaccines |
US7771995B2 (en) | 2005-11-14 | 2010-08-10 | Merial Limited | Plasmid encoding human BMP-7 |
US7598364B2 (en) | 2005-11-14 | 2009-10-06 | Merial Limited | Plasmid encoding canine BMP-7 |
BRPI0709282B8 (pt) | 2006-03-29 | 2021-05-25 | Merial Ltd | vacina contra estreptococos |
US7862821B2 (en) | 2006-06-01 | 2011-01-04 | Merial Limited | Recombinant vaccine against bluetongue virus |
EP2116599B1 (fr) | 2006-11-24 | 2014-08-06 | Waseda University | Réactif pour l'introduction d'une protéine ou d'un gène |
SI2147105T1 (sl) | 2007-05-02 | 2013-08-30 | Merial Limited | DNA-plazmidi z izboljšano ekspresijo in stabilnostjo |
US20090111099A1 (en) * | 2007-10-27 | 2009-04-30 | Yongsheng Ma | Promoter Detection and Analysis |
EP2283035B1 (fr) | 2008-05-08 | 2015-07-29 | Merial Limited | Vaccin contre la leishmaniose utilisant un immunogène salivaire de phlébotome |
MX2011005604A (es) | 2008-11-28 | 2011-09-01 | Merial Ltd | Vacuna recombinante para influenza aviar y sus usos. |
CN102428099B (zh) | 2009-04-03 | 2016-03-16 | 梅里亚有限公司 | 运载新城疫病毒的禽疫苗 |
ES2600612T3 (es) | 2009-05-22 | 2017-02-10 | Merial, Inc. | Plásmido libre de antibióticos |
CA2785653C (fr) | 2009-12-28 | 2018-05-01 | Merial Limited | Antigene recombinant du ndv, et ses utilisations |
US20130197612A1 (en) | 2010-02-26 | 2013-08-01 | Jack W. Lasersohn | Electromagnetic Radiation Therapy |
US20110236416A1 (en) | 2010-03-12 | 2011-09-29 | Jean-Christophe Audonnet | Foot and mouth disease virus recombinant vaccines and uses thereof |
JP5913316B2 (ja) | 2010-08-31 | 2016-04-27 | メリアル リミテッド | ニューカッスル病ウイルスをベクターとするヘルペスウイルスワクチン |
US9216213B2 (en) | 2011-04-20 | 2015-12-22 | Merial, Inc. | Adjuvanted rabies vaccine with improved viscosity profile |
EP2714722B1 (fr) | 2011-05-27 | 2019-09-11 | Boehringer Ingelheim Animal Health USA Inc. | Vaccins genetiques contre les virus hendra et nipah |
CA2837375C (fr) | 2011-06-01 | 2019-07-16 | Merial Limited | Administration sans aiguille de vaccins contre le vsrrp |
LT2734230T (lt) | 2011-07-20 | 2019-03-25 | Merial Limited | Rekombinantinė kačių leikemijos viruso vakcina, turinti optimizuotą kačių leukemijos viruso apvalkalo geną |
US10166188B2 (en) | 2011-08-12 | 2019-01-01 | Merial, Inc. | Method for vacuum-assisted preservation of biologics including vaccines |
CN104203271B (zh) | 2012-02-14 | 2017-08-25 | 梅里亚股份有限公司 | 表达狂犬病病毒和ox40蛋白的重组痘病毒载体及从其制造的疫苗 |
EP2814508B1 (fr) | 2012-02-14 | 2017-04-12 | Merial, Inc. | Vaccins sous-unitaires contre le rotavirus, procedés de fabrication et leur utilisation |
CA2867893C (fr) | 2012-03-20 | 2022-09-20 | Merial Limited | Vaccin contre l'herpesvirus equin 1 recombinant contenant une glycoproteine c mutee et utilisations associees |
WO2013143555A1 (fr) | 2012-03-26 | 2013-10-03 | Biontech Ag | Formulation d'arn pour immunothérapie |
RU2656187C2 (ru) | 2012-06-13 | 2018-05-31 | Мериал, Инк. | Реассортантные btv и ahsv вакцины |
KR102319843B1 (ko) | 2013-09-25 | 2021-10-29 | 조에티스 서비시즈 엘엘씨 | Pcv2b 분지형 백신 조성물 및 사용 방법 |
NZ741421A (en) | 2014-05-14 | 2023-05-26 | Vitamfero S A | Methods for freeze-drying and rehydrating biologics |
BR112017027895A2 (pt) | 2015-06-23 | 2018-11-06 | Merial Inc | vetores virais recombinantes contendo proteínas menores de prrsv e métodos de fabricação e utilização destes |
US10617752B2 (en) | 2015-06-26 | 2020-04-14 | Boehringer Ingelheim Animal Health USA Inc. | Inactivated canine influenza vaccines and methods of making and uses thereof |
EP4286002A3 (fr) | 2015-09-29 | 2024-02-28 | Boehringer Ingelheim Animal Health USA Inc. | Vaccins à particules pseudo-virales (vlp) de parvovirus canin (cpv) et utilisations associées |
DK3380119T3 (da) | 2015-11-23 | 2021-11-15 | Boehringer Ingelheim Animal Health Usa Inc | Fmdv- og e2-fusionsproteiner og anvendelser deraf |
TWI760322B (zh) | 2016-01-29 | 2022-04-11 | 美商百靈佳殷格翰動物保健美國有限公司 | 重組腺病毒載體裝載之fmdv疫苗及其用途 |
AU2019258679A1 (en) * | 2018-04-25 | 2020-10-15 | Ethris Gmbh | Cryoprotective agents for particulate formulations |
JP7271026B1 (ja) * | 2021-12-28 | 2023-05-11 | 株式会社Hyperion Drug Discovery | 遺伝子導入細胞の免疫応答を回避させるための剤 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1990003808A1 (fr) * | 1988-10-07 | 1990-04-19 | The Liposome Company, Inc. | Traitement thermique de liposomes |
US5399346A (en) * | 1989-06-14 | 1995-03-21 | The United States Of America As Represented By The Department Of Health And Human Services | Gene therapy |
US5264618A (en) * | 1990-04-19 | 1993-11-23 | Vical, Inc. | Cationic lipids for intracellular delivery of biologically active molecules |
DE4131562A1 (de) * | 1991-09-18 | 1993-03-25 | Medac Klinische Spezialpraep | Arzneistofftraeger aus festen lipidteilchen-feste lipidnanosphaeren (sln) |
-
1996
- 1996-04-11 CA CA002214029A patent/CA2214029A1/fr not_active Abandoned
- 1996-04-11 WO PCT/US1996/005035 patent/WO1996034109A1/fr not_active Application Discontinuation
- 1996-04-11 EP EP96911722A patent/EP0826063A1/fr not_active Withdrawn
- 1996-04-11 JP JP8532559A patent/JPH11504631A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
WO1996034109A1 (fr) | 1996-10-31 |
EP0826063A1 (fr) | 1998-03-04 |
JPH11504631A (ja) | 1999-04-27 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FZDE | Dead |