CA2141430C - Amplification d'une sequence d'acide nucleique - Google Patents

Amplification d'une sequence d'acide nucleique Download PDF

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Publication number
CA2141430C
CA2141430C CA002141430A CA2141430A CA2141430C CA 2141430 C CA2141430 C CA 2141430C CA 002141430 A CA002141430 A CA 002141430A CA 2141430 A CA2141430 A CA 2141430A CA 2141430 C CA2141430 C CA 2141430C
Authority
CA
Canada
Prior art keywords
oligonucleotide
modification
sequence
primer sequence
promoter
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA002141430A
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English (en)
Other versions
CA2141430A1 (fr
Inventor
Sherrol H. Mcdonough
Daniel L. Kacian
Nanibhushan Dattagupta
Diane L. Mcallister
Philip W. Hammond
Thomas B. Ryder
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gen Probe Inc
Original Assignee
Gen Probe Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Probe Inc filed Critical Gen Probe Inc
Publication of CA2141430A1 publication Critical patent/CA2141430A1/fr
Application granted granted Critical
Publication of CA2141430C publication Critical patent/CA2141430C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/35—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Mycobacteriaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
CA002141430A 1992-08-04 1993-07-28 Amplification d'une sequence d'acide nucleique Expired - Lifetime CA2141430C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US92540592A 1992-08-04 1992-08-04
US07/925,405 1992-08-04
PCT/US1993/007138 WO1994003472A1 (fr) 1992-08-04 1993-07-28 Amplification de sequence d'acides nucleiques

Publications (2)

Publication Number Publication Date
CA2141430A1 CA2141430A1 (fr) 1994-02-17
CA2141430C true CA2141430C (fr) 2009-05-12

Family

ID=25451694

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002141430A Expired - Lifetime CA2141430C (fr) 1992-08-04 1993-07-28 Amplification d'une sequence d'acide nucleique

Country Status (11)

Country Link
EP (2) EP0587298B1 (fr)
JP (3) JP4372837B2 (fr)
KR (1) KR100316498B1 (fr)
AT (2) ATE197479T1 (fr)
AU (2) AU670116B2 (fr)
CA (1) CA2141430C (fr)
DE (2) DE69329636T2 (fr)
ES (1) ES2277679T3 (fr)
NO (1) NO950381L (fr)
TW (1) TW278099B (fr)
WO (1) WO1994003472A1 (fr)

Families Citing this family (78)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5981179A (en) * 1991-11-14 1999-11-09 Digene Diagnostics, Inc. Continuous amplification reaction
WO1995015399A1 (fr) 1993-12-01 1995-06-08 Toyo Boseki Kabushiki Kaisha Procede d'amplification et de detection d'une sequence nucleotidique au moyen d'enzymes thermostables
FR2724934B1 (fr) * 1994-09-26 1997-01-24 Bio Merieux Oligonucleotide chimere et son utilisation dans l'obtention de transcrits d'un acide nucleique
US5514551A (en) * 1994-10-14 1996-05-07 Gen-Probe Incorporated Compositions for the detection of Chlamydia trachomatis
DK0805876T3 (da) * 1995-01-19 2000-11-06 Gen Probe Inc Nukleinsyreamplifikationsoligonukleotider og prober til Lyme-sygdom-associeret Borrelia
US5622827A (en) * 1995-02-07 1997-04-22 Gen-Probe Incorporated Amplification primers and nucleic acid probes targeted to coccidioides immitis nucleic acid
AU693023B2 (en) 1995-05-05 1998-06-18 Applied Biosystems, Llc Methods and reagents for combined PCR amplification and hybridization probing assay
US5925518A (en) * 1995-05-19 1999-07-20 Akzo Nobel N.V. Nucleic acid primers for amplification of a mycobacteria RNA template
WO1996040990A1 (fr) * 1995-06-07 1996-12-19 Gen-Probe Incorporated Procedes et trousses servant a determiner les niveaux de preamplification d'une sequence cible d'acides nucleiques a partir des niveaux de post-amplification du produit
AU707691B2 (en) * 1995-06-07 1999-07-15 Gen-Probe Incorporated Nucleic acid probes and amplification oligonucleotides for neisseria species
US5882857A (en) * 1995-06-07 1999-03-16 Behringwerke Ag Internal positive controls for nucleic acid amplification
US5739311A (en) * 1995-06-07 1998-04-14 Gen-Probe Incorporated Enzymatic synthesis of phosphorothioate oligonucleotides using restriction endonucleases
US5652126A (en) * 1995-06-07 1997-07-29 Gen-Probe Incorporated Use of restriction endonuclease sequences for cleaving phosphorothioate oligonucleotides
US5710029A (en) * 1995-06-07 1998-01-20 Gen-Probe Incorporated Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5747252A (en) 1995-06-07 1998-05-05 Gen-Probe Incorporated Nucleic acid probes and amplification oligonucleotides for Neisseria species
US5932450A (en) * 1995-06-07 1999-08-03 Gen-Probe Incorporated Enzymatic synthesis of oligonucleotides using digestible templates
US5705365A (en) * 1995-06-07 1998-01-06 Gen-Probe Incorporated Kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5916777A (en) * 1995-06-07 1999-06-29 Gen-Probe Incorporated Enzymatic synthesis of oligonucleotides using 3'-ribonucleotide primers
WO1997032044A1 (fr) * 1996-03-01 1997-09-04 E.I. Du Pont De Nemours And Company Procede d'amplification et de detection d'un fragment d'acide nucleique d'interet
US6218107B1 (en) 1996-05-22 2001-04-17 Gen-Probe Incorporated Compositions and methods for detecting the presence of Mycobacterium kansassii
US6013488A (en) * 1996-07-25 2000-01-11 The Institute Of Physical And Chemical Research Method for reverse transcription
US6057134A (en) * 1996-10-07 2000-05-02 Ambion, Inc. Modulating the efficiency of nucleic acid amplification reactions with 3' modified oligonucleotides
WO1998022625A1 (fr) 1996-11-20 1998-05-28 The Regents Of The University Of Michigan Dispositifs microfabriques et procedes d'amplification isotherme d'acides nucleiques
GB9626074D0 (en) * 1996-12-16 1997-02-05 Cytocell Ltd Nucleic acids amplification assay
US6025133A (en) * 1996-12-30 2000-02-15 Gen-Probe Incorporated Promoter-sequestered oligonucleoside and method of use
ES2271994T3 (es) * 1997-05-02 2007-04-16 Gen-Probe Incorporated Hibridacion en dos pasos y captura de un polinucleotido.
US6534273B2 (en) 1997-05-02 2003-03-18 Gen-Probe Incorporated Two-step hybridization and capture of a polynucleotide
DE69833864T2 (de) * 1997-08-14 2006-12-21 Takara Bio Inc., Otsu Methoden zur dns vervielfältigung und material dafür
JP2002509443A (ja) * 1997-10-31 2002-03-26 ジェン−プローブ・インコーポレーテッド 核酸検出法
US5932451A (en) * 1997-11-19 1999-08-03 Incyte Pharmaceuticals, Inc. Method for unbiased mRNA amplification
JP4438110B2 (ja) * 1998-07-01 2010-03-24 東ソー株式会社 標的核酸の定量方法
AU4739600A (en) * 1999-05-17 2000-12-05 Mcgill University Method for subtracting cdnas by suppressing the synthesis of specifically targeted mrnas
DE60022541T2 (de) 1999-07-23 2006-06-14 Gen Probe Inc Polynukleotid- amplifikationsverfahren
US6489114B2 (en) 1999-12-17 2002-12-03 Bio Merieux Process for labeling a ribonucleic acid, and labeled RNA fragments which are obtained thereby
US6902891B2 (en) 1999-12-17 2005-06-07 Bio Merieux Process for labeling a nucleic acid
JP2002051778A (ja) * 2000-05-31 2002-02-19 Tosoh Corp 小型球形ウイルス（ｓｒｓｖ）ｒｎａ検出のためのオリゴヌクレオチドおよび検出法
AUPR050700A0 (en) * 2000-10-03 2000-10-26 Id+Plus Ltd Detection method
AU2001291513B2 (en) * 2000-10-03 2007-06-07 Id+Plus Ltd Nucleotide detection method
WO2002034951A2 (fr) 2000-10-23 2002-05-02 Gen-Probe Incorporated Compositions et procedes de detection du virus de l'immunodeficience humaine 2 (vih-2)
JP2002125688A (ja) * 2000-10-30 2002-05-08 Tosoh Corp Ｃ型肝炎ウイルスの高感度検出のためのオリゴヌクレオチドおよび検出法
DE10119203B4 (de) * 2001-04-19 2004-09-16 Rolf Dr. Postina Verfahren zur Erzeugung von doppelsträngigen Nukleinsäuren mit einzelsträngigen Überhängen beliebiger Länge und Nukleotidzusammensetzung
EP2292801B1 (fr) 2002-06-14 2018-08-08 Gen-Probe Incorporated Compositions et procédé pour la détection du virus de l'hépatite b
EP2977470B1 (fr) 2002-10-16 2018-01-03 Gen-Probe Incorporated Compositions et procédé pour la détection du virus du nil occidental
EP1694871B1 (fr) 2003-12-19 2010-08-25 Gen-Probe Incorporated Compositions, methodes et trousses destinees a la detection des acides nucleiques du vih 1 et du vih 2
CA2847930C (fr) 2004-09-30 2016-07-05 Gen-Probe Incorporated Test de detection et de quantification du vih-1
AU2005333163B2 (en) 2004-11-09 2011-06-16 Gen-Probe Incorporated Compositions and methods for detecting Group A streptococci
JP4819064B2 (ja) 2005-02-18 2011-11-16 ジェン−プロウブ インコーポレイテッド アルカリショックを取り入れたサンプル調製法
EP1877418B1 (fr) 2005-05-06 2013-11-20 Gen-Probe Incorporated Compositions et analyses permettant de detecter des acides nucleiques des virus a de la grippe
WO2007059179A1 (fr) 2005-11-14 2007-05-24 Gen-Probe Incorporated Procede d’etalonnage de parametres
EP2027293B1 (fr) * 2006-05-24 2014-11-19 Gen-Probe Incorporated Methode pour le lysage de mycobacterium et l'amplification de ses acides nucleiques
EP1921156A1 (fr) * 2006-11-10 2008-05-14 bioMerieux B.V. Amplification multiplexe améliorée d'acides nucléiques au moyen d' amorces bloquées
GB0701253D0 (en) 2007-01-23 2007-02-28 Diagnostics For The Real World Nucleic acid amplification and testing
EP1978111B1 (fr) * 2007-04-02 2013-03-27 Gen-Probe Incorporated Compositions, kits et procédés associés pour la détection et/ou la surveillance de Pseudomonas aeruginosa
EP3392351A1 (fr) 2008-04-21 2018-10-24 Gen-Probe Incorporated Procédé de détection de virus du chikungunya
AU2010217928B2 (en) 2009-02-26 2013-06-06 Gen-Probe Incorporated Assay for detection of human parvovirus nucleic acid
EP3674423A1 (fr) 2010-07-12 2020-07-01 Gen-Probe Incorporated Compositions et tests permettant de détecter l'acide nucléique du virus de la grippe a saisonnière h3
EP2611932A2 (fr) 2010-08-30 2013-07-10 Gen-Probe Incorporated Compositions, procédés et mélanges réactionnels pour détection du virus xénotrope apparenté aux virus de la leucémie murine
AU2012284307A1 (en) 2011-07-15 2013-05-09 Gen-Probe Incorporated Compositions and method for detecting human parvovirus nucleic acid and for detecting hepatitis A virus nucleic acids in single-plex or multiplex assays
EP2877952B1 (fr) 2012-07-27 2023-04-12 Gen-Probe Incorporated Procédé d'étalonnage à double référence et système de quantification de polynucléotides
ES2795677T3 (es) 2013-03-15 2020-11-24 Gen Probe Inc Método, aparato y producto de programa informático para calibración
CA3176529C (fr) 2017-03-24 2024-03-05 Gen-Probe Incorporated Compositions et procedes de detection d'agents pathogenes viraux dans des echantillons
US10844425B2 (en) 2017-03-24 2020-11-24 Gen-Probe Incorporated Compositions and methods for detecting or quantifying parainfluenza virus
EP4219766A3 (fr) 2017-08-11 2023-10-11 Gen-Probe Incorporated Compositions et procédés de détection de staphylococcus aureus
WO2019118735A1 (fr) 2017-12-15 2019-06-20 Gen-Probe Incorporated Compositions et méthodes pour la détection de clostridium difficile toxigène
GB2597569B (en) 2018-06-13 2023-01-25 Gen Probe Inc Compositions and methods for detecting group B streptococcus nucleic acid
EP3830302B1 (fr) 2018-08-01 2022-10-05 Gen-Probe Incorporated Compositions et procédés de détection d'acides nucléiques du virus d'epstein-barr
JP2021532808A (ja) 2018-08-08 2021-12-02 ジェン−プローブ・インコーポレーテッド Ｍｙｃｏｐｌａｓｍａ ｇｅｎｉｔａｌｉｕｍを検出するための組成物、方法、およびキット
CA3107043A1 (fr) 2018-08-21 2020-02-27 Gen-Probe Incorporated Compositions et procedes d'amplification, de detection ou de quantification de cytomegalovirus humain
WO2020069085A2 (fr) 2018-09-27 2020-04-02 Gen-Probe Incorporated Compositions et procédés de détection d'acides nucléiques de bordetella pertussis et de bordetella parapertussis
WO2020086546A1 (fr) 2018-10-22 2020-04-30 Gen-Probe Incorporated Compositions et procédés d'amplification, de détection ou de quantification de polyomavirus bk humain
CA3152309A1 (fr) 2019-08-23 2021-03-04 Gen-Probe Incorporated Compositions, procedes et kits pour la detection de treponema pallidum
EP4025715A1 (fr) 2019-09-05 2022-07-13 Gen-Probe Incorporated Détection de variants d'acide nucléique de chlamydia trachomatis
EP4073268B1 (fr) 2019-12-09 2024-04-10 Gen-Probe Incorporated Quantification d'analytes polynucléotidiques à partir d'échantillons séchés
JP2023516683A (ja) 2020-03-04 2023-04-20 ジェン－プローブ・インコーポレーテッド ＳＡＲＳ－ＣｏＶ－２核酸を検出するための組成物および方法
AU2022319876A1 (en) 2021-07-27 2024-01-18 Gen-Probe Incorporated Compositions and methods for detecting gastrointestinal pathogens
KR102544380B1 (ko) 2022-01-29 2023-06-16 (주)동방지퍼 지퍼용 슬라이더와 손잡이의 결착방법 및 이로 인해 제조된 지퍼
EP4282980A1 (fr) 2022-05-23 2023-11-29 Mobidiag Oy Procédés d'amplification d'un acide nucléique
WO2024054924A1 (fr) 2022-09-08 2024-03-14 Gen-Probe Incorporated Procédé de détection d'analytes d'acides nucléiques à l'aide d'amorces à double spécificité

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988003957A1 (fr) * 1986-11-24 1988-06-02 Gen-Probe Incorporated Sondes d'acide nucleique pour la detection et/ou la quantification d'organismes non viraux
IL86724A (en) * 1987-06-19 1995-01-24 Siska Diagnostics Inc Methods and kits for amplification and testing of nucleic acid sequences
US4994368A (en) * 1987-07-23 1991-02-19 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
EP0731175A3 (fr) * 1989-07-11 2004-05-26 Gen-Probe Incorporated Sondes oligonucléotidiques pour d'acide nucléique de VIH
CA2035010C (fr) * 1990-01-26 1996-12-10 Keith C. Backman Methode d'amplification des acides nucleiques cibles applicable aux reactions en chaine a la polymerase et a la ligase
WO1992007957A1 (fr) * 1990-10-31 1992-05-14 Cimino George D Procedes et compositions servant a reduire au minimum l'inhibition de l'amplification d'acides nucleiques
US5169766A (en) * 1991-06-14 1992-12-08 Life Technologies, Inc. Amplification of nucleic acid molecules
US5521300A (en) * 1991-08-13 1996-05-28 Norval B. Galloway Oligonucleotides complementary to mycobacterial nucleic acids
CA2135073C (fr) * 1992-05-06 2002-11-19 Daniel L. Kacian Procede, composition et trousse d'amplification des sequences d'acides nucleiques

Also Published As

Publication number Publication date
CA2141430A1 (fr) 1994-02-17
TW278099B (fr) 1996-06-11
NO950381D0 (no) 1995-02-02
AU6802796A (en) 1996-12-19
AU670116B2 (en) 1996-07-04
DE69334092D1 (de) 2007-01-11
EP0587298A2 (fr) 1994-03-16
KR950702999A (ko) 1995-08-23
KR100316498B1 (ko) 2002-06-20
EP0587298B1 (fr) 2000-11-08
EP0587298A3 (en) 1995-08-30
DE69329636T2 (de) 2001-03-01
NO950381L (no) 1995-04-03
JPH07509368A (ja) 1995-10-19
AU700253B2 (en) 1998-12-24
AU4792093A (en) 1994-03-03
JP2006238892A (ja) 2006-09-14
DE69329636D1 (de) 2000-12-14
ATE346954T1 (de) 2006-12-15
JP4372837B2 (ja) 2009-11-25
EP0978570B1 (fr) 2006-11-29
DE69334092T2 (de) 2007-07-05
EP0978570A2 (fr) 2000-02-09
WO1994003472A1 (fr) 1994-02-17
ES2277679T3 (es) 2007-07-16
JP3860809B2 (ja) 2006-12-20
EP0978570A3 (fr) 2000-04-12
ATE197479T1 (de) 2000-11-11
JP2004194662A (ja) 2004-07-15

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