CA2061569A1 - Activation de proteines recombinantes - Google Patents
Activation de proteines recombinantesInfo
- Publication number
- CA2061569A1 CA2061569A1 CA2061569A CA2061569A CA2061569A1 CA 2061569 A1 CA2061569 A1 CA 2061569A1 CA 2061569 A CA2061569 A CA 2061569A CA 2061569 A CA2061569 A CA 2061569A CA 2061569 A1 CA2061569 A1 CA 2061569A1
- Authority
- CA
- Canada
- Prior art keywords
- recombinant proteins
- amino acids
- improved activation
- helper sequences
- relative hydrophobicities
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/53—Colony-stimulating factor [CSF]
- C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/06—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length using protecting groups or activating agents
- C07K1/08—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length using protecting groups or activating agents using activating agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
- C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Analytical Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- General Chemical & Material Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Plant Pathology (AREA)
- Toxicology (AREA)
- Microbiology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Dental Preparations (AREA)
- Manufacture Of Macromolecular Shaped Articles (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Adhesives Or Adhesive Processes (AREA)
- Nitrogen- Or Sulfur-Containing Heterocyclic Ring Compounds With Rings Of Six Or More Members (AREA)
- Adhesive Tapes (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DEP4105480.6 | 1991-02-21 | ||
DE4105480A DE4105480A1 (de) | 1991-02-21 | 1991-02-21 | Verbesserte aktivierung von rekombinanten proteinen |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2061569A1 true CA2061569A1 (fr) | 1992-08-22 |
CA2061569C CA2061569C (fr) | 2000-10-24 |
Family
ID=6425597
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002061569A Expired - Fee Related CA2061569C (fr) | 1991-02-21 | 1992-02-20 | Activation de proteines recombinantes |
Country Status (20)
Country | Link |
---|---|
US (1) | US5578710A (fr) |
EP (1) | EP0500108B1 (fr) |
JP (1) | JP2528232B2 (fr) |
KR (1) | KR950014493B1 (fr) |
AT (1) | ATE144284T1 (fr) |
AU (1) | AU641081B2 (fr) |
CA (1) | CA2061569C (fr) |
CZ (1) | CZ282744B6 (fr) |
DE (2) | DE4105480A1 (fr) |
DK (1) | DK0500108T3 (fr) |
ES (1) | ES2093122T3 (fr) |
FI (1) | FI106029B (fr) |
GR (1) | GR3021395T3 (fr) |
HU (1) | HU214881B (fr) |
IE (1) | IE920276A1 (fr) |
IL (1) | IL101024A (fr) |
MX (1) | MX9200709A (fr) |
NO (1) | NO300329B1 (fr) |
NZ (1) | NZ241570A (fr) |
ZA (1) | ZA921230B (fr) |
Families Citing this family (25)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5718893A (en) * | 1984-04-15 | 1998-02-17 | Foster; Preston F. | Use of G-CSF to reduce acute rejection |
US5581476A (en) | 1993-01-28 | 1996-12-03 | Amgen Inc. | Computer-based methods and articles of manufacture for preparing G-CSF analogs |
SE9301057L (sv) * | 1993-03-30 | 1994-10-01 | Pharmacia Ab | Beredning med kontrollerad frisättning |
US5536495A (en) * | 1994-04-15 | 1996-07-16 | Foster; Preston F. | Use of G-CSF to reduce acute rejection |
US20030053982A1 (en) | 1994-09-26 | 2003-03-20 | Kinstler Olaf B. | N-terminally chemically modified protein compositions and methods |
US5824784A (en) | 1994-10-12 | 1998-10-20 | Amgen Inc. | N-terminally chemically modified protein compositions and methods |
US6245740B1 (en) | 1998-12-23 | 2001-06-12 | Amgen Inc. | Polyol:oil suspensions for the sustained release of proteins |
WO2002020767A2 (fr) | 2000-09-08 | 2002-03-14 | Massachusetts Institute Of Technology | Composition d'analogue du g-csf et procede associe |
HUP0303199A2 (hu) | 2001-02-19 | 2003-12-29 | Merck Patent Gmbh | Eljárás T-sejt-epitópok azonosítására és csökkentett immunogenitású molekulák előállítására |
MXPA04000231A (es) | 2001-07-11 | 2004-05-04 | Maxygen Holdings Ltd | Conjugados del factor de estimulacion de colonias de granulocitos. |
WO2003078461A1 (fr) | 2002-03-20 | 2003-09-25 | Biopolymed Inc. | Preparation d'un g-csf stoechiometriquement conjugue a des polymeres biocompatibles au niveau d'un residu de cysteine |
US7666627B2 (en) * | 2002-08-08 | 2010-02-23 | Targetex Kft. | Folded recombinant catalytic fragments of multidomain serine proteases, preparation and uses thereof |
US7785601B2 (en) | 2002-12-31 | 2010-08-31 | Sygnis Bioscience Gmbh & Co. Kg | Methods of treating neurological conditions with hematopoietic growth factors |
US7695723B2 (en) | 2002-12-31 | 2010-04-13 | Sygnis Bioscience Gmbh & Co. Kg | Methods of treating neurological conditions with hematopoietic growth factors |
US7220407B2 (en) | 2003-10-27 | 2007-05-22 | Amgen Inc. | G-CSF therapy as an adjunct to reperfusion therapy in the treatment of acute myocardial infarction |
WO2006055260A2 (fr) | 2004-11-05 | 2006-05-26 | Northwestern University | Utilisation de scf et de scf dans le traitement de l'ischemie cerebrale et des troubles neurologiques |
CA2607844C (fr) | 2005-06-01 | 2012-07-10 | Maxygen Holdings Ltd. | Polypeptides g-csf pegyles et procedes de production correspondants |
CL2007002166A1 (es) * | 2006-07-24 | 2008-01-25 | Tetralogic Pharm Corp | Compuestos derivados de heterociclos de nitrogeno, antagonistas de los inhibidores de las proteinas de la apoptosis; sus composiciones farmaceuticas; y uso de dichos compuestos para el tratamiento del cancer. |
EP2581441A1 (fr) | 2007-08-09 | 2013-04-17 | Genzyme Corporation | Procédé de traitement d'une maladie auto-immune avec des cellules souches mésenchymateuses |
US8758761B2 (en) | 2007-09-30 | 2014-06-24 | University Of Florida Research Foundation, Inc. | Combination therapies for treating type 1 diabetes |
US8283372B2 (en) | 2009-07-02 | 2012-10-09 | Tetralogic Pharmaceuticals Corp. | 2-(1H-indol-3-ylmethyl)-pyrrolidine dimer as a SMAC mimetic |
NZ602255A (en) | 2010-03-04 | 2014-04-30 | Pfenex Inc | Method for producing soluble recombinant interferon protein without denaturing |
CN102892780B (zh) * | 2010-03-17 | 2015-07-29 | 通益制药有限公司 | 获得生物活性的重组人g-csf的方法 |
PL2552949T3 (pl) | 2010-04-01 | 2017-01-31 | Pfenex Inc. | Sposoby wytwarzania G-CSF w komórce gospodarza Pseudomonas |
ES2633894T3 (es) * | 2012-08-02 | 2017-09-25 | F. Hoffmann-La Roche Ag | Procedimiento para producir moléculas monoméricas y multiméricas y usos de las mismas |
Family Cites Families (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4532207A (en) * | 1982-03-19 | 1985-07-30 | G. D. Searle & Co. | Process for the preparation of polypeptides utilizing a charged amino acid polymer and exopeptidase |
DK55685A (da) * | 1985-02-07 | 1986-08-08 | Nordisk Gentofte | Enzym eller enzymkompleks med proteolytisk aktivitet |
US4530787A (en) * | 1984-03-28 | 1985-07-23 | Cetus Corporation | Controlled oxidation of microbially produced cysteine-containing proteins |
US5082775A (en) * | 1984-05-11 | 1992-01-21 | Berlex Laboratories, Inc. | Efficient process for isolating insoluble heterologous protein using non-ionic detergents |
US4948729A (en) * | 1985-03-25 | 1990-08-14 | Cetus Corporation | Production of soluble recombinant proteins |
US4783415A (en) * | 1985-03-28 | 1988-11-08 | Meiji Seika Kabushiki Kaisha | Gene coding for signal peptides and utilization thereof |
US5087564A (en) * | 1985-06-20 | 1992-02-11 | Monsanto Company | Release of recombinant peptides from polypeptides using V8 endopeptidase |
DE3636903A1 (de) * | 1985-12-21 | 1987-07-02 | Hoechst Ag | Fusionsproteine mit eukaryotischem ballastanteil |
JPH0618781B2 (ja) * | 1986-10-18 | 1994-03-16 | 中外製薬株式会社 | 感染症治療剤 |
EP0305500B1 (fr) * | 1987-03-20 | 1994-11-09 | Creative Biomolecules, Inc. | Procede de purification de polypeptides recombinants |
US5013653A (en) * | 1987-03-20 | 1991-05-07 | Creative Biomolecules, Inc. | Product and process for introduction of a hinge region into a fusion protein to facilitate cleavage |
JPH03503596A (ja) * | 1987-06-24 | 1991-08-15 | ノボ・ノルディスク エー/エス | 蛋白質若しくはポリペプチドの調製方法、該ポリペプチドをコードするdna配列、該dna配列およびポリペプチドを有する微生物および該ポリペプチドの薬学的製剤としての利用 |
DE3835350A1 (de) * | 1988-10-17 | 1990-04-19 | Boehringer Mannheim Gmbh | Aktivierung von gentechnologisch hergestellten, in prokaryonten exprimierten antikoerpern |
ZA901719B (en) * | 1989-03-19 | 1991-01-30 | Akzo Nv | Hog cholera virus vaccine and diagnostics |
US5191063A (en) * | 1989-05-02 | 1993-03-02 | University Of Medicine And Dentistry Of New Jersey | Production of biologically active polypeptides by treatment with an exogenous peptide sequence |
US5115102A (en) * | 1989-07-21 | 1992-05-19 | Monsanto Company | Variant proteins and polypeptides possessing enhanced affinity for immobilized-metal affinity matrices |
ATE154073T1 (de) * | 1990-08-20 | 1997-06-15 | Novo Nordisk As | Prozess für die herstellung von biologisch aktivem igf-1 durch verwendung von amino-terminal verlängertem igf-1 |
-
1991
- 1991-02-21 DE DE4105480A patent/DE4105480A1/de not_active Withdrawn
-
1992
- 1992-01-28 IE IE027692A patent/IE920276A1/en not_active IP Right Cessation
- 1992-02-10 NZ NZ241570A patent/NZ241570A/en not_active IP Right Cessation
- 1992-02-14 AU AU10948/92A patent/AU641081B2/en not_active Ceased
- 1992-02-20 HU HU9200548A patent/HU214881B/hu not_active IP Right Cessation
- 1992-02-20 MX MX9200709A patent/MX9200709A/es not_active IP Right Cessation
- 1992-02-20 ES ES92102864T patent/ES2093122T3/es not_active Expired - Lifetime
- 1992-02-20 CZ CS92499A patent/CZ282744B6/cs not_active IP Right Cessation
- 1992-02-20 FI FI920742A patent/FI106029B/fi not_active IP Right Cessation
- 1992-02-20 CA CA002061569A patent/CA2061569C/fr not_active Expired - Fee Related
- 1992-02-20 DE DE59207351T patent/DE59207351D1/de not_active Revoked
- 1992-02-20 DK DK92102864.3T patent/DK0500108T3/da active
- 1992-02-20 EP EP92102864A patent/EP0500108B1/fr not_active Revoked
- 1992-02-20 AT AT92102864T patent/ATE144284T1/de not_active IP Right Cessation
- 1992-02-20 NO NO920671A patent/NO300329B1/no unknown
- 1992-02-20 IL IL10102492A patent/IL101024A/en not_active IP Right Cessation
- 1992-02-20 ZA ZA921230A patent/ZA921230B/xx unknown
- 1992-02-20 JP JP4033257A patent/JP2528232B2/ja not_active Expired - Lifetime
- 1992-02-21 KR KR1019920002697A patent/KR950014493B1/ko not_active IP Right Cessation
-
1993
- 1993-10-21 US US08/139,054 patent/US5578710A/en not_active Expired - Lifetime
-
1996
- 1996-10-17 GR GR960402469T patent/GR3021395T3/el unknown
Also Published As
Publication number | Publication date |
---|---|
HUT68021A (en) | 1995-04-04 |
FI920742A0 (fi) | 1992-02-20 |
CA2061569C (fr) | 2000-10-24 |
EP0500108B1 (fr) | 1996-10-16 |
KR920016464A (ko) | 1992-09-24 |
NO300329B1 (no) | 1997-05-12 |
EP0500108A3 (en) | 1993-04-07 |
ZA921230B (en) | 1992-11-25 |
IL101024A0 (en) | 1992-11-15 |
FI920742A (fi) | 1992-08-22 |
CS49992A3 (en) | 1992-09-16 |
GR3021395T3 (en) | 1997-01-31 |
FI106029B (fi) | 2000-11-15 |
AU1094892A (en) | 1992-08-27 |
KR950014493B1 (ko) | 1995-12-02 |
HU214881B (hu) | 1998-07-28 |
CZ282744B6 (cs) | 1997-09-17 |
DE59207351D1 (de) | 1996-11-21 |
NO920671L (no) | 1992-08-24 |
US5578710A (en) | 1996-11-26 |
IE920276A1 (en) | 1992-08-26 |
MX9200709A (es) | 1992-09-01 |
DK0500108T3 (da) | 1997-03-24 |
NZ241570A (en) | 1994-09-27 |
IL101024A (en) | 1996-06-18 |
JP2528232B2 (ja) | 1996-08-28 |
ES2093122T3 (es) | 1996-12-16 |
NO920671D0 (no) | 1992-02-20 |
DE4105480A1 (de) | 1992-08-27 |
HU9200548D0 (en) | 1992-04-28 |
EP0500108A2 (fr) | 1992-08-26 |
JPH05244977A (ja) | 1993-09-24 |
AU641081B2 (en) | 1993-09-09 |
ATE144284T1 (de) | 1996-11-15 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKLA | Lapsed |