CA2006008A1 - Methode pour obtenir des oligonucleotides synthetiques qui se lient specifiquement a des sites cibles sur des molecules d'adn double brin, en formant un triplex colineaire; methode d'utilisation de ces oligonucleotides synthetiques - Google Patents
Methode pour obtenir des oligonucleotides synthetiques qui se lient specifiquement a des sites cibles sur des molecules d'adn double brin, en formant un triplex colineaire; methode d'utilisation de ces oligonucleotides synthetiquesInfo
- Publication number
- CA2006008A1 CA2006008A1 CA2006008A CA2006008A CA2006008A1 CA 2006008 A1 CA2006008 A1 CA 2006008A1 CA 2006008 A CA2006008 A CA 2006008A CA 2006008 A CA2006008 A CA 2006008A CA 2006008 A1 CA2006008 A1 CA 2006008A1
- Authority
- CA
- Canada
- Prior art keywords
- synthetic oligonucleotides
- dna
- bind
- duplex
- duplex dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108091034117 Oligonucleotide Proteins 0.000 title abstract 11
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title abstract 11
- 238000000034 method Methods 0.000 title abstract 9
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 abstract 4
- 230000000295 complement effect Effects 0.000 abstract 3
- 239000002773 nucleotide Substances 0.000 abstract 2
- 125000003729 nucleotide group Chemical group 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 206010028980 Neoplasm Diseases 0.000 abstract 1
- CZPWVGJYEJSRLH-UHFFFAOYSA-N Pyrimidine Chemical compound C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 abstract 1
- 201000011510 cancer Diseases 0.000 abstract 1
- 230000010261 cell growth Effects 0.000 abstract 1
- 201000010099 disease Diseases 0.000 abstract 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 230000002194 synthesizing effect Effects 0.000 abstract 1
Classifications
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
- C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
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- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
- C12N15/1138—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against receptors or cell surface proteins
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- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01034—Hydroxymethylglutaryl-CoA reductase (NADPH) (1.1.1.34)
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- C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
- C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
- C12Y205/01018—Glutathione transferase (2.5.1.18)
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- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/32—Chemical structure of the sugar
- C12N2310/321—2'-O-R Modification
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Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US28735988A | 1988-12-20 | 1988-12-20 | |
US287,359 | 1988-12-20 |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2006008A1 true CA2006008A1 (fr) | 1990-06-20 |
CA2006008C CA2006008C (fr) | 2000-02-15 |
Family
ID=23102554
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002006008A Expired - Fee Related CA2006008C (fr) | 1988-12-20 | 1989-12-19 | Methode pour obtenir des oligonucleotides synthetiques qui se lient specifiquement a des sites cibles sur des molecules d'adn double brin, en formant un triplex colineaire; methode d'utilisation de ces oligonucleotides synthetiques |
Country Status (10)
Country | Link |
---|---|
EP (2) | EP0449972A4 (fr) |
JP (1) | JPH04502407A (fr) |
AT (1) | ATE118818T1 (fr) |
AU (1) | AU640910B2 (fr) |
CA (1) | CA2006008C (fr) |
DE (1) | DE68921315T2 (fr) |
DK (1) | DK120091A (fr) |
ES (1) | ES2069598T3 (fr) |
PT (1) | PT92640B (fr) |
WO (1) | WO1990006934A1 (fr) |
Families Citing this family (118)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5422251A (en) * | 1986-11-26 | 1995-06-06 | Princeton University | Triple-stranded nucleic acids |
US5928863A (en) * | 1986-11-26 | 1999-07-27 | Princeton University | Triple-stranded nucleic acids |
US5849482A (en) * | 1988-09-28 | 1998-12-15 | Epoch Pharmaceuticals, Inc. | Crosslinking oligonucleotides |
US5721218A (en) * | 1989-10-23 | 1998-02-24 | Gilead Sciences, Inc. | Oligonucleotides with inverted polarity |
US5399676A (en) * | 1989-10-23 | 1995-03-21 | Gilead Sciences | Oligonucleotides with inverted polarity |
IE20010427A1 (en) * | 1990-04-10 | 2003-03-05 | Canji Inc | Gene therapy for cell proliferative diseases |
EP0531436A4 (en) * | 1990-05-25 | 1993-06-16 | Gilead Sciences, Inc. | Sequence-specific nonphotoactivated crosslinking agents which bind to the major groove of duplex dna |
EP0463712A3 (en) * | 1990-06-27 | 1992-04-08 | University Patents, Inc. | Polynucleotide phosphorodithioates as therapeutic agents for retroviral infections |
US5734022A (en) * | 1990-08-01 | 1998-03-31 | The Johns Hopkins University | Antibodies to a novel mammalian protein associated with uncontrolled cell division |
EP0558634A4 (en) * | 1990-11-23 | 1995-06-28 | Gilead Sciences Inc | Triplex-forming oligomers containing modified bases |
WO1992011390A1 (fr) * | 1990-12-17 | 1992-07-09 | Idexx Laboratories, Inc. | Detection de sequences d'acide nucleique par la formation d'une triple helice de l'adn |
US5683874A (en) * | 1991-03-27 | 1997-11-04 | Research Corporation Technologies, Inc. | Single-stranded circular oligonucleotides capable of forming a triplex with a target sequence |
US6136601A (en) * | 1991-08-21 | 2000-10-24 | Epoch Pharmaceuticals, Inc. | Targeted mutagenesis in living cells using modified oligonucleotides |
CA2116343A1 (fr) * | 1991-08-30 | 1993-03-18 | Paul S. Miller | Formulation de complexes a triple helice d'adn double brin avec des oligomeres de nucleosides contenant des analogues de bases puriques |
IL103311A0 (en) * | 1991-10-07 | 1993-03-15 | Univ Johns Hopkins | Formation of triple helix complexes of single stranded nucleic acids using nucleoside oligomers |
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-
1989
- 1989-12-19 CA CA002006008A patent/CA2006008C/fr not_active Expired - Fee Related
- 1989-12-20 EP EP19900901460 patent/EP0449972A4/en active Pending
- 1989-12-20 EP EP89313391A patent/EP0375408B1/fr not_active Expired - Lifetime
- 1989-12-20 AU AU48384/90A patent/AU640910B2/en not_active Ceased
- 1989-12-20 JP JP2502252A patent/JPH04502407A/ja active Pending
- 1989-12-20 WO PCT/US1989/005769 patent/WO1990006934A1/fr not_active Application Discontinuation
- 1989-12-20 AT AT89313391T patent/ATE118818T1/de not_active IP Right Cessation
- 1989-12-20 ES ES89313391T patent/ES2069598T3/es not_active Expired - Lifetime
- 1989-12-20 DE DE68921315T patent/DE68921315T2/de not_active Expired - Fee Related
- 1989-12-20 PT PT92640A patent/PT92640B/pt not_active IP Right Cessation
-
1991
- 1991-06-20 DK DK120091A patent/DK120091A/da not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
AU640910B2 (en) | 1993-09-09 |
EP0375408B1 (fr) | 1995-02-22 |
JPH04502407A (ja) | 1992-05-07 |
ATE118818T1 (de) | 1995-03-15 |
WO1990006934A1 (fr) | 1990-06-28 |
PT92640B (pt) | 1995-09-12 |
DE68921315D1 (de) | 1995-03-30 |
CA2006008C (fr) | 2000-02-15 |
AU4838490A (en) | 1990-07-10 |
DE68921315T2 (de) | 1995-08-03 |
ES2069598T3 (es) | 1995-05-16 |
DK120091D0 (da) | 1991-06-20 |
PT92640A (pt) | 1990-06-29 |
EP0449972A4 (en) | 1992-03-18 |
EP0449972A1 (fr) | 1991-10-09 |
DK120091A (da) | 1991-06-20 |
EP0375408A1 (fr) | 1990-06-27 |
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