BRPI9816263B1 - método de transformação de uma célula vegetal com um gene de interesse e de preparação de uma planta transgênica fértil - Google Patents
método de transformação de uma célula vegetal com um gene de interesse e de preparação de uma planta transgênica fértil Download PDFInfo
- Publication number
- BRPI9816263B1 BRPI9816263B1 BRPI9816263A BR9816263A BRPI9816263B1 BR PI9816263 B1 BRPI9816263 B1 BR PI9816263B1 BR PI9816263 A BRPI9816263 A BR PI9816263A BR 9816263 A BR9816263 A BR 9816263A BR PI9816263 B1 BRPI9816263 B1 BR PI9816263B1
- Authority
- BR
- Brazil
- Prior art keywords
- agrobacterium
- callus
- cells
- embryos
- gene
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 82
- 238000000034 method Methods 0.000 title claims abstract description 44
- 230000009261 transgenic effect Effects 0.000 title claims abstract description 17
- 230000001131 transforming effect Effects 0.000 title claims abstract description 9
- 241000589158 Agrobacterium Species 0.000 claims abstract description 185
- 241000196324 Embryophyta Species 0.000 claims abstract description 93
- 230000017074 necrotic cell death Effects 0.000 claims abstract description 35
- 230000002401 inhibitory effect Effects 0.000 claims abstract description 17
- 240000008042 Zea mays Species 0.000 claims description 106
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 103
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 claims description 53
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 45
- 235000005822 corn Nutrition 0.000 claims description 45
- 230000009466 transformation Effects 0.000 claims description 39
- 229910001961 silver nitrate Inorganic materials 0.000 claims description 26
- 239000013598 vector Substances 0.000 claims description 25
- 239000003112 inhibitor Substances 0.000 claims description 21
- 239000003795 chemical substances by application Substances 0.000 claims description 18
- 239000000041 non-steroidal anti-inflammatory agent Substances 0.000 claims description 13
- 229940021182 non-steroidal anti-inflammatory drug Drugs 0.000 claims description 13
- 230000008929 regeneration Effects 0.000 claims description 12
- 238000011069 regeneration method Methods 0.000 claims description 12
- 239000013000 chemical inhibitor Substances 0.000 claims description 11
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 claims description 10
- 239000005977 Ethylene Substances 0.000 claims description 10
- 241000209140 Triticum Species 0.000 claims description 7
- 235000021307 Triticum Nutrition 0.000 claims description 7
- 238000002360 preparation method Methods 0.000 claims description 5
- ZSILVJLXKHGNPL-UHFFFAOYSA-L S(=S)(=O)([O-])[O-].[Ag+2] Chemical compound S(=S)(=O)([O-])[O-].[Ag+2] ZSILVJLXKHGNPL-UHFFFAOYSA-L 0.000 claims description 4
- SJYNFBVQFBRSIB-UHFFFAOYSA-N norbornadiene Chemical group C1=CC2C=CC1C2 SJYNFBVQFBRSIB-UHFFFAOYSA-N 0.000 claims description 4
- JFNLZVQOOSMTJK-KNVOCYPGSA-N norbornene Chemical compound C1[C@@H]2CC[C@H]1C=C2 JFNLZVQOOSMTJK-KNVOCYPGSA-N 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims 1
- 241000209504 Poaceae Species 0.000 abstract description 13
- 210000004027 cell Anatomy 0.000 description 133
- 206010020649 Hyperkeratosis Diseases 0.000 description 126
- 239000002609 medium Substances 0.000 description 105
- 210000001519 tissue Anatomy 0.000 description 102
- 210000002257 embryonic structure Anatomy 0.000 description 93
- 238000011081 inoculation Methods 0.000 description 59
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 58
- 235000009973 maize Nutrition 0.000 description 58
- 108020004414 DNA Proteins 0.000 description 54
- 230000000408 embryogenic effect Effects 0.000 description 49
- 239000013612 plasmid Substances 0.000 description 32
- 230000000977 initiatory effect Effects 0.000 description 31
- 108010060309 Glucuronidase Proteins 0.000 description 26
- 102000053187 Glucuronidase Human genes 0.000 description 26
- 230000030833 cell death Effects 0.000 description 25
- 230000000694 effects Effects 0.000 description 24
- 102400000124 Cyclin-dependent kinase 5 activator 1, p35 Human genes 0.000 description 23
- 239000012634 fragment Substances 0.000 description 19
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 18
- 238000013467 fragmentation Methods 0.000 description 18
- 238000006062 fragmentation reaction Methods 0.000 description 18
- 230000000692 anti-sense effect Effects 0.000 description 17
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 16
- 210000004436 artificial bacterial chromosome Anatomy 0.000 description 16
- 229960004261 cefotaxime Drugs 0.000 description 16
- AZZMGZXNTDTSME-JUZDKLSSSA-M cefotaxime sodium Chemical compound [Na+].N([C@@H]1C(N2C(=C(COC(C)=O)CS[C@@H]21)C([O-])=O)=O)C(=O)\C(=N/OC)C1=CSC(N)=N1 AZZMGZXNTDTSME-JUZDKLSSSA-M 0.000 description 16
- 230000014509 gene expression Effects 0.000 description 16
- 238000003782 apoptosis assay Methods 0.000 description 15
- 230000006907 apoptotic process Effects 0.000 description 15
- 238000002474 experimental method Methods 0.000 description 15
- 239000007788 liquid Substances 0.000 description 15
- 230000005522 programmed cell death Effects 0.000 description 15
- 238000006243 chemical reaction Methods 0.000 description 14
- 108020004707 nucleic acids Proteins 0.000 description 14
- 102000039446 nucleic acids Human genes 0.000 description 14
- 150000007523 nucleic acids Chemical class 0.000 description 14
- 230000035939 shock Effects 0.000 description 14
- 238000012360 testing method Methods 0.000 description 14
- 108091026890 Coding region Proteins 0.000 description 13
- 241000588724 Escherichia coli Species 0.000 description 13
- 229930006000 Sucrose Natural products 0.000 description 13
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 13
- 239000002585 base Substances 0.000 description 13
- 229960004793 sucrose Drugs 0.000 description 13
- 239000002773 nucleotide Substances 0.000 description 12
- 125000003729 nucleotide group Chemical group 0.000 description 12
- 239000005720 sucrose Substances 0.000 description 12
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 11
- YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 11
- IAJOBQBIJHVGMQ-UHFFFAOYSA-N Phosphinothricin Natural products CP(O)(=O)CCC(N)C(O)=O IAJOBQBIJHVGMQ-UHFFFAOYSA-N 0.000 description 11
- IAJOBQBIJHVGMQ-BYPYZUCNSA-N glufosinate-P Chemical compound CP(O)(=O)CC[C@H](N)C(O)=O IAJOBQBIJHVGMQ-BYPYZUCNSA-N 0.000 description 11
- 238000012423 maintenance Methods 0.000 description 11
- 241000894006 Bacteria Species 0.000 description 10
- 230000015572 biosynthetic process Effects 0.000 description 10
- 239000000872 buffer Substances 0.000 description 10
- 102000004169 proteins and genes Human genes 0.000 description 10
- 230000004044 response Effects 0.000 description 10
- 102000004190 Enzymes Human genes 0.000 description 9
- 108090000790 Enzymes Proteins 0.000 description 9
- 229940088598 enzyme Drugs 0.000 description 9
- 210000001161 mammalian embryo Anatomy 0.000 description 9
- 108010082527 phosphinothricin N-acetyltransferase Proteins 0.000 description 9
- WWAABJGNHFGXSJ-UHFFFAOYSA-N chlorophenol red Chemical compound C1=C(Cl)C(O)=CC=C1C1(C=2C=C(Cl)C(O)=CC=2)C2=CC=CC=C2S(=O)(=O)O1 WWAABJGNHFGXSJ-UHFFFAOYSA-N 0.000 description 8
- 238000004520 electroporation Methods 0.000 description 8
- 235000018102 proteins Nutrition 0.000 description 8
- 239000000523 sample Substances 0.000 description 8
- 239000011780 sodium chloride Substances 0.000 description 8
- 230000010474 transient expression Effects 0.000 description 8
- 101150077138 virB1 gene Proteins 0.000 description 8
- 229920000936 Agarose Polymers 0.000 description 7
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 7
- 230000001580 bacterial effect Effects 0.000 description 7
- 230000001338 necrotic effect Effects 0.000 description 7
- 238000003757 reverse transcription PCR Methods 0.000 description 7
- 230000004083 survival effect Effects 0.000 description 7
- 239000000725 suspension Substances 0.000 description 7
- 230000001018 virulence Effects 0.000 description 7
- JLIDBLDQVAYHNE-YKALOCIXSA-N Abscisic acid Natural products OC(=O)/C=C(/C)\C=C\[C@@]1(O)C(C)=CC(=O)CC1(C)C JLIDBLDQVAYHNE-YKALOCIXSA-N 0.000 description 6
- 229920001817 Agar Polymers 0.000 description 6
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 6
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 6
- 241000208125 Nicotiana Species 0.000 description 6
- 239000008272 agar Substances 0.000 description 6
- 238000002635 electroconvulsive therapy Methods 0.000 description 6
- 208000015181 infectious disease Diseases 0.000 description 6
- 239000003550 marker Substances 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 238000012216 screening Methods 0.000 description 6
- 238000003786 synthesis reaction Methods 0.000 description 6
- 108020004705 Codon Proteins 0.000 description 5
- IMXSCCDUAFEIOE-UHFFFAOYSA-N D-Octopin Natural products OC(=O)C(C)NC(C(O)=O)CCCN=C(N)N IMXSCCDUAFEIOE-UHFFFAOYSA-N 0.000 description 5
- IMXSCCDUAFEIOE-RITPCOANSA-N D-octopine Chemical compound [O-]C(=O)[C@@H](C)[NH2+][C@H](C([O-])=O)CCCNC(N)=[NH2+] IMXSCCDUAFEIOE-RITPCOANSA-N 0.000 description 5
- 238000007792 addition Methods 0.000 description 5
- 230000002424 anti-apoptotic effect Effects 0.000 description 5
- 238000003556 assay Methods 0.000 description 5
- 230000003115 biocidal effect Effects 0.000 description 5
- 229940041514 candida albicans extract Drugs 0.000 description 5
- 238000011065 in-situ storage Methods 0.000 description 5
- 230000007246 mechanism Effects 0.000 description 5
- 238000007747 plating Methods 0.000 description 5
- 230000002829 reductive effect Effects 0.000 description 5
- 230000035945 sensitivity Effects 0.000 description 5
- 238000012546 transfer Methods 0.000 description 5
- 238000011282 treatment Methods 0.000 description 5
- 239000012138 yeast extract Substances 0.000 description 5
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 4
- HSSBORCLYSCBJR-UHFFFAOYSA-N Chloramben Chemical compound NC1=CC(Cl)=CC(C(O)=O)=C1Cl HSSBORCLYSCBJR-UHFFFAOYSA-N 0.000 description 4
- 108010067770 Endopeptidase K Proteins 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 4
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 4
- 108091022912 Mannose-6-Phosphate Isomerase Proteins 0.000 description 4
- 206010028980 Neoplasm Diseases 0.000 description 4
- 239000001888 Peptone Substances 0.000 description 4
- 108010080698 Peptones Proteins 0.000 description 4
- 210000004102 animal cell Anatomy 0.000 description 4
- YPHMISFOHDHNIV-FSZOTQKASA-N cycloheximide Chemical compound C1[C@@H](C)C[C@H](C)C(=O)[C@@H]1[C@H](O)CC1CC(=O)NC(=O)C1 YPHMISFOHDHNIV-FSZOTQKASA-N 0.000 description 4
- 239000000499 gel Substances 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 4
- 229910052737 gold Inorganic materials 0.000 description 4
- 239000010931 gold Substances 0.000 description 4
- 230000012010 growth Effects 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 230000002363 herbicidal effect Effects 0.000 description 4
- 239000004009 herbicide Substances 0.000 description 4
- 238000009396 hybridization Methods 0.000 description 4
- 238000011534 incubation Methods 0.000 description 4
- 230000001939 inductive effect Effects 0.000 description 4
- 238000002955 isolation Methods 0.000 description 4
- 229930027917 kanamycin Natural products 0.000 description 4
- 229960000318 kanamycin Drugs 0.000 description 4
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 4
- 229930182823 kanamycin A Natural products 0.000 description 4
- 108010083942 mannopine synthase Proteins 0.000 description 4
- 101150113864 pat gene Proteins 0.000 description 4
- 235000019319 peptone Nutrition 0.000 description 4
- YBYRMVIVWMBXKQ-UHFFFAOYSA-N phenylmethanesulfonyl fluoride Chemical compound FS(=O)(=O)CC1=CC=CC=C1 YBYRMVIVWMBXKQ-UHFFFAOYSA-N 0.000 description 4
- 230000010152 pollination Effects 0.000 description 4
- 239000002244 precipitate Substances 0.000 description 4
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 4
- -1 silver nitrate Chemical class 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 102000048193 Mannose-6-phosphate isomerases Human genes 0.000 description 3
- 108010014251 Muramidase Proteins 0.000 description 3
- 102000016943 Muramidase Human genes 0.000 description 3
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 3
- 108091034117 Oligonucleotide Proteins 0.000 description 3
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 3
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 3
- 238000002105 Southern blotting Methods 0.000 description 3
- 108090000848 Ubiquitin Proteins 0.000 description 3
- 102000044159 Ubiquitin Human genes 0.000 description 3
- 241000700605 Viruses Species 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 230000004913 activation Effects 0.000 description 3
- 101150103518 bar gene Proteins 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 230000029087 digestion Effects 0.000 description 3
- 238000001962 electrophoresis Methods 0.000 description 3
- 230000002068 genetic effect Effects 0.000 description 3
- 230000006698 induction Effects 0.000 description 3
- 230000003993 interaction Effects 0.000 description 3
- 229960000274 lysozyme Drugs 0.000 description 3
- 239000004325 lysozyme Substances 0.000 description 3
- 235000010335 lysozyme Nutrition 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000006798 recombination Effects 0.000 description 3
- 238000005215 recombination Methods 0.000 description 3
- 230000001172 regenerating effect Effects 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 238000010186 staining Methods 0.000 description 3
- 238000004114 suspension culture Methods 0.000 description 3
- 230000001960 triggered effect Effects 0.000 description 3
- 239000013638 trimer Substances 0.000 description 3
- 239000012137 tryptone Substances 0.000 description 3
- 238000011144 upstream manufacturing Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- USGUVNUTPWXWBA-JRIXXDKMSA-N (e,2s)-2-amino-4-(2-aminoethoxy)but-3-enoic acid Chemical compound NCCO\C=C\[C@H](N)C(O)=O USGUVNUTPWXWBA-JRIXXDKMSA-N 0.000 description 2
- OVSKIKFHRZPJSS-UHFFFAOYSA-N 2,4-D Chemical compound OC(=O)COC1=CC=C(Cl)C=C1Cl OVSKIKFHRZPJSS-UHFFFAOYSA-N 0.000 description 2
- HXKWSTRRCHTUEC-UHFFFAOYSA-N 2,4-Dichlorophenoxyaceticacid Chemical compound OC(=O)C(Cl)OC1=CC=C(Cl)C=C1 HXKWSTRRCHTUEC-UHFFFAOYSA-N 0.000 description 2
- ISPYQTSUDJAMAB-UHFFFAOYSA-N 2-chlorophenol Chemical compound OC1=CC=CC=C1Cl ISPYQTSUDJAMAB-UHFFFAOYSA-N 0.000 description 2
- 241000589156 Agrobacterium rhizogenes Species 0.000 description 2
- BSYNRYMUTXBXSQ-UHFFFAOYSA-N Aspirin Chemical compound CC(=O)OC1=CC=CC=C1C(O)=O BSYNRYMUTXBXSQ-UHFFFAOYSA-N 0.000 description 2
- 208000003643 Callosities Diseases 0.000 description 2
- 108010066133 D-octopine dehydrogenase Proteins 0.000 description 2
- 101000785279 Dictyostelium discoideum Calcium-transporting ATPase PAT1 Proteins 0.000 description 2
- 101001129314 Dictyostelium discoideum Probable plasma membrane ATPase Proteins 0.000 description 2
- 208000035240 Disease Resistance Diseases 0.000 description 2
- 108010042407 Endonucleases Proteins 0.000 description 2
- 102000004533 Endonucleases Human genes 0.000 description 2
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- 229930191978 Gibberellin Natural products 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- 101000779309 Homo sapiens Amyloid protein-binding protein 2 Proteins 0.000 description 2
- 101000713296 Homo sapiens Proton-coupled amino acid transporter 1 Proteins 0.000 description 2
- 101000713293 Homo sapiens Proton-coupled amino acid transporter 2 Proteins 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 108090000157 Metallothionein Proteins 0.000 description 2
- 102000003992 Peroxidases Human genes 0.000 description 2
- 102100036920 Proton-coupled amino acid transporter 1 Human genes 0.000 description 2
- 102100036919 Proton-coupled amino acid transporter 2 Human genes 0.000 description 2
- 101100327927 Rhizobium radiobacter chvD gene Proteins 0.000 description 2
- 108700025695 Suppressor Genes Proteins 0.000 description 2
- 239000004098 Tetracycline Substances 0.000 description 2
- 235000007244 Zea mays Nutrition 0.000 description 2
- 229960001138 acetylsalicylic acid Drugs 0.000 description 2
- 108010045649 agarase Proteins 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- 238000013019 agitation Methods 0.000 description 2
- 230000004075 alteration Effects 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000005557 antagonist Substances 0.000 description 2
- 230000001640 apoptogenic effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 150000001768 cations Chemical class 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- YTRQFSDWAXHJCC-UHFFFAOYSA-N chloroform;phenol Chemical compound ClC(Cl)Cl.OC1=CC=CC=C1 YTRQFSDWAXHJCC-UHFFFAOYSA-N 0.000 description 2
- 150000001868 cobalt Chemical class 0.000 description 2
- 230000009089 cytolysis Effects 0.000 description 2
- 230000034994 death Effects 0.000 description 2
- 229960003964 deoxycholic acid Drugs 0.000 description 2
- FCRACOPGPMPSHN-UHFFFAOYSA-N desoxyabscisic acid Natural products OC(=O)C=C(C)C=CC1C(C)=CC(=O)CC1(C)C FCRACOPGPMPSHN-UHFFFAOYSA-N 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 210000005069 ears Anatomy 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000013604 expression vector Substances 0.000 description 2
- 239000008098 formaldehyde solution Substances 0.000 description 2
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 2
- 239000003448 gibberellin Substances 0.000 description 2
- 101150054900 gus gene Proteins 0.000 description 2
- 101150047832 hpt gene Proteins 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 239000000411 inducer Substances 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 231100000518 lethal Toxicity 0.000 description 2
- 230000001665 lethal effect Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000001404 mediated effect Effects 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 108020004999 messenger RNA Proteins 0.000 description 2
- 208000024191 minimally invasive lung adenocarcinoma Diseases 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 239000008188 pellet Substances 0.000 description 2
- 108040007629 peroxidase activity proteins Proteins 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 108091008146 restriction endonucleases Proteins 0.000 description 2
- 108700022109 ropocamptide Proteins 0.000 description 2
- 229960004889 salicylic acid Drugs 0.000 description 2
- FHHPUSMSKHSNKW-SMOYURAASA-M sodium deoxycholate Chemical compound [Na+].C([C@H]1CC2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC([O-])=O)C)[C@@]2(C)[C@@H](O)C1 FHHPUSMSKHSNKW-SMOYURAASA-M 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 229960000268 spectinomycin Drugs 0.000 description 2
- UNFWWIHTNXNPBV-WXKVUWSESA-N spectinomycin Chemical compound O([C@@H]1[C@@H](NC)[C@@H](O)[C@H]([C@@H]([C@H]1O1)O)NC)[C@]2(O)[C@H]1O[C@H](C)CC2=O UNFWWIHTNXNPBV-WXKVUWSESA-N 0.000 description 2
- ATHGHQPFGPMSJY-UHFFFAOYSA-N spermidine Chemical compound NCCCCNCCCN ATHGHQPFGPMSJY-UHFFFAOYSA-N 0.000 description 2
- 229960002180 tetracycline Drugs 0.000 description 2
- 229930101283 tetracycline Natural products 0.000 description 2
- 235000019364 tetracycline Nutrition 0.000 description 2
- 150000003522 tetracyclines Chemical class 0.000 description 2
- 230000005740 tumor formation Effects 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- CAAMSDWKXXPUJR-UHFFFAOYSA-N 3,5-dihydro-4H-imidazol-4-one Chemical compound O=C1CNC=N1 CAAMSDWKXXPUJR-UHFFFAOYSA-N 0.000 description 1
- HCWLJSDMOMMDRF-SZWOQXJISA-N 3-[(3s,6r)-2-oxo-6-[(1s,2r,3r)-1,2,3,4-tetrahydroxybutyl]morpholin-3-yl]propanamide Chemical compound NC(=O)CC[C@@H]1NC[C@H]([C@@H](O)[C@H](O)[C@H](O)CO)OC1=O HCWLJSDMOMMDRF-SZWOQXJISA-N 0.000 description 1
- 108010020183 3-phosphoshikimate 1-carboxyvinyltransferase Proteins 0.000 description 1
- 101710103719 Acetolactate synthase large subunit Proteins 0.000 description 1
- 101710182467 Acetolactate synthase large subunit IlvB1 Proteins 0.000 description 1
- 101710171176 Acetolactate synthase large subunit IlvG Proteins 0.000 description 1
- 101710176702 Acetolactate synthase small subunit Proteins 0.000 description 1
- 101710147947 Acetolactate synthase small subunit 1, chloroplastic Proteins 0.000 description 1
- 101710095712 Acetolactate synthase, mitochondrial Proteins 0.000 description 1
- ILQOASSPNGAIBC-UHFFFAOYSA-N Agropine Natural products OC(O)C(O)CC(O)C1CN2C(CCC2=O)C(=O)O1 ILQOASSPNGAIBC-UHFFFAOYSA-N 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 108020005544 Antisense RNA Proteins 0.000 description 1
- 229940088872 Apoptosis inhibitor Drugs 0.000 description 1
- 241000219194 Arabidopsis Species 0.000 description 1
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
- 241000972773 Aulopiformes Species 0.000 description 1
- 101100394813 Bacillus subtilis (strain 168) hprT gene Proteins 0.000 description 1
- 241000193388 Bacillus thuringiensis Species 0.000 description 1
- 101100062377 Caenorhabditis elegans dad-1 gene Proteins 0.000 description 1
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 235000002566 Capsicum Nutrition 0.000 description 1
- 241000143940 Colias Species 0.000 description 1
- 241000186031 Corynebacteriaceae Species 0.000 description 1
- LMKYZBGVKHTLTN-NKWVEPMBSA-N D-nopaline Chemical compound NC(=N)NCCC[C@@H](C(O)=O)N[C@@H](C(O)=O)CCC(O)=O LMKYZBGVKHTLTN-NKWVEPMBSA-N 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 238000007399 DNA isolation Methods 0.000 description 1
- 239000003298 DNA probe Substances 0.000 description 1
- 208000001840 Dandruff Diseases 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 102000016911 Deoxyribonucleases Human genes 0.000 description 1
- 108010053770 Deoxyribonucleases Proteins 0.000 description 1
- 101710162371 Dihydroxy-acid dehydratase 1 Proteins 0.000 description 1
- 102100039104 Dolichyl-diphosphooligosaccharide-protein glycosyltransferase subunit DAD1 Human genes 0.000 description 1
- 101710178850 Dolichyl-diphosphooligosaccharide-protein glycosyltransferase subunit DAD1 Proteins 0.000 description 1
- 102100031780 Endonuclease Human genes 0.000 description 1
- 241000588722 Escherichia Species 0.000 description 1
- 101001091269 Escherichia coli Hygromycin-B 4-O-kinase Proteins 0.000 description 1
- 241001302160 Escherichia coli str. K-12 substr. DH10B Species 0.000 description 1
- 108700007698 Genetic Terminator Regions Proteins 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 239000005562 Glyphosate Substances 0.000 description 1
- 101710172804 K protein Proteins 0.000 description 1
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 description 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 description 1
- VPRLICVDSGMIKO-UHFFFAOYSA-N Mannopine Natural products NC(=O)CCC(C(O)=O)NCC(O)C(O)C(O)C(O)CO VPRLICVDSGMIKO-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 108700020796 Oncogene Proteins 0.000 description 1
- 102000043276 Oncogene Human genes 0.000 description 1
- 108700026244 Open Reading Frames Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 239000006002 Pepper Substances 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 108091000080 Phosphotransferase Proteins 0.000 description 1
- 235000016761 Piper aduncum Nutrition 0.000 description 1
- 235000017804 Piper guineense Nutrition 0.000 description 1
- 244000203593 Piper nigrum Species 0.000 description 1
- 235000008184 Piper nigrum Nutrition 0.000 description 1
- 101710196435 Probable acetolactate synthase large subunit Proteins 0.000 description 1
- 101710181764 Probable acetolactate synthase small subunit Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 101710104000 Putative acetolactate synthase small subunit Proteins 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 241000220317 Rosa Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 101001091268 Streptomyces hygroscopicus Hygromycin-B 7''-O-kinase Proteins 0.000 description 1
- 208000037065 Subacute sclerosing leukoencephalitis Diseases 0.000 description 1
- 206010042297 Subacute sclerosing panencephalitis Diseases 0.000 description 1
- 229940100389 Sulfonylurea Drugs 0.000 description 1
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- 241000219094 Vitaceae Species 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 229940024606 amino acid Drugs 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000000074 antisense oligonucleotide Substances 0.000 description 1
- 238000012230 antisense oligonucleotides Methods 0.000 description 1
- 239000000158 apoptosis inhibitor Substances 0.000 description 1
- 230000005735 apoptotic response Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 229960001230 asparagine Drugs 0.000 description 1
- 235000009582 asparagine Nutrition 0.000 description 1
- 229940097012 bacillus thuringiensis Drugs 0.000 description 1
- 108700042656 bcl-1 Genes Proteins 0.000 description 1
- GINJFDRNADDBIN-FXQIFTODSA-N bilanafos Chemical compound OC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](N)CCP(C)(O)=O GINJFDRNADDBIN-FXQIFTODSA-N 0.000 description 1
- 238000010804 cDNA synthesis Methods 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 235000011148 calcium chloride Nutrition 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- 101150055766 cat gene Proteins 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000008618 cell wall macromolecule catabolic process Effects 0.000 description 1
- 229960005091 chloramphenicol Drugs 0.000 description 1
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 230000010428 chromatin condensation Effects 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 101150012914 chvG gene Proteins 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000001934 delay Effects 0.000 description 1
- 230000003544 deproteinization Effects 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000013681 dietary sucrose Nutrition 0.000 description 1
- 230000003292 diminished effect Effects 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 239000012458 free base Substances 0.000 description 1
- 102000034356 gene-regulatory proteins Human genes 0.000 description 1
- 108091006104 gene-regulatory proteins Proteins 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 description 1
- 229940097068 glyphosate Drugs 0.000 description 1
- 235000021021 grapes Nutrition 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 108091006086 inhibitor proteins Proteins 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 description 1
- 229960001669 kinetin Drugs 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 229910001425 magnesium ion Inorganic materials 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- VPRLICVDSGMIKO-SZWOQXJISA-N mannopine Chemical compound NC(=O)CC[C@@H](C(O)=O)NC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO VPRLICVDSGMIKO-SZWOQXJISA-N 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 210000004088 microvessel Anatomy 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000004660 morphological change Effects 0.000 description 1
- 101150057066 nahG gene Proteins 0.000 description 1
- 230000000952 necrogenic effect Effects 0.000 description 1
- 150000002823 nitrates Chemical class 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 235000021317 phosphate Nutrition 0.000 description 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- BLFWHYXWBKKRHI-JYBILGDPSA-N plap Chemical compound N([C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCC(O)=O)C(=O)N1[C@@H](CCC1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(=O)[C@@H]1CCCN1C(=O)[C@H](CO)NC(=O)[C@@H](N)CCC(O)=O BLFWHYXWBKKRHI-JYBILGDPSA-N 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 229910001414 potassium ion Inorganic materials 0.000 description 1
- 230000003405 preventing effect Effects 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 235000019419 proteases Nutrition 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000019515 salmon Nutrition 0.000 description 1
- 230000011218 segmentation Effects 0.000 description 1
- 230000010153 self-pollination Effects 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- 229910001415 sodium ion Inorganic materials 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 229940063673 spermidine Drugs 0.000 description 1
- 238000009987 spinning Methods 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- YROXIXLRRCOBKF-UHFFFAOYSA-N sulfonylurea Chemical compound OC(=N)N=S(=O)=O YROXIXLRRCOBKF-UHFFFAOYSA-N 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 239000003104 tissue culture media Substances 0.000 description 1
- 230000008467 tissue growth Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 108700012359 toxins Proteins 0.000 description 1
- 238000011426 transformation method Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 230000007332 vesicle formation Effects 0.000 description 1
- 101150007883 virD1 gene Proteins 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
- C12N15/8205—Agrobacterium mediated transformation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/14011—Baculoviridae
- C12N2710/14022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/582—Recycling of unreacted starting or intermediate materials
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Virology (AREA)
- Botany (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Compounds Of Unknown Constitution (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US86786997A | 1997-06-02 | 1997-06-02 | |
| PCT/EP1998/003215 WO1998054961A2 (en) | 1997-06-02 | 1998-05-29 | Plant transformation methods |
| BRPI9809899A BRPI9809899B8 (pt) | 1997-06-02 | 1998-05-29 | métodos de transformação de uma célula vegetal graminieae sp. com um gene de interesse e para preparação de uma planta transgênica fértil. |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BRPI9816263B1 true BRPI9816263B1 (pt) | 2016-08-16 |
Family
ID=25350632
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI9816263A BRPI9816263B1 (pt) | 1997-06-02 | 1998-05-29 | método de transformação de uma célula vegetal com um gene de interesse e de preparação de uma planta transgênica fértil |
| BRPI9809899A BRPI9809899B8 (pt) | 1997-06-02 | 1998-05-29 | métodos de transformação de uma célula vegetal graminieae sp. com um gene de interesse e para preparação de uma planta transgênica fértil. |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI9809899A BRPI9809899B8 (pt) | 1997-06-02 | 1998-05-29 | métodos de transformação de uma célula vegetal graminieae sp. com um gene de interesse e para preparação de uma planta transgênica fértil. |
Country Status (19)
| Country | Link |
|---|---|
| EP (1) | EP0986299B1 (enExample) |
| JP (1) | JP2002502252A (enExample) |
| KR (1) | KR100505910B1 (enExample) |
| CN (1) | CN1150320C (enExample) |
| AR (1) | AR015837A1 (enExample) |
| AT (1) | ATE336890T1 (enExample) |
| AU (1) | AU735472B2 (enExample) |
| BR (2) | BRPI9816263B1 (enExample) |
| CA (1) | CA2290863C (enExample) |
| DE (1) | DE69835672T2 (enExample) |
| HU (1) | HU226212B1 (enExample) |
| IL (2) | IL132768A0 (enExample) |
| MX (1) | MX257801B (enExample) |
| PL (1) | PL336979A1 (enExample) |
| RU (1) | RU2226549C2 (enExample) |
| TR (2) | TR199902941T2 (enExample) |
| UA (1) | UA72443C2 (enExample) |
| WO (1) | WO1998054961A2 (enExample) |
| ZA (1) | ZA984681B (enExample) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3331367B2 (ja) | 1997-03-11 | 2002-10-07 | 独立行政法人農業生物資源研究所 | 細胞死抑制遺伝子が導入されたストレス抵抗性植物およびその作出方法 |
| JP2002538769A (ja) * | 1998-10-30 | 2002-11-19 | ユニバーシティ オブ ネブラスカ−リンカーン | アポトーシス遺伝子の異種間移入及びそれにより開発されたトランスジェニック植物 |
| US6603061B1 (en) | 1999-07-29 | 2003-08-05 | Monsanto Company | Agrobacterium-mediated plant transformation method |
| WO2001044459A2 (en) * | 1999-12-15 | 2001-06-21 | Regents Of The University Of Minnesota | Method to enhance agrobacterium-mediated transformation of plants |
| US6570069B1 (en) * | 2000-02-10 | 2003-05-27 | Regents Of The University Of California | Nucleic acids encoding plant inhibitors of apoptosis and transgenic cells and plants expressing them |
| US7468475B2 (en) | 2000-06-16 | 2008-12-23 | Schmuelling Thomas | Method for modifying plant morphology, biochemistry and physiology |
| CN1273604C (zh) * | 2000-08-03 | 2006-09-06 | 日本烟草产业株式会社 | 提高植物细胞转基因效率的方法 |
| EP2298919A1 (en) | 2003-04-11 | 2011-03-23 | CropDesign N.V. | Method to increase stress tolerance in plants |
| KR100671225B1 (ko) | 2005-01-06 | 2007-01-19 | 전남대학교산학협력단 | 글리신-풍부 ｒｎａ-결합 단백질을 암호화하는뉴클레오타이드 서열을 이용하여 식물에 저온 또는 동결내성을 부여하는 방법 |
| GB0525645D0 (en) * | 2005-12-16 | 2006-01-25 | Plant Bioscience Ltd | Methods, means and compositions for enhancing agrobacterium-mediated plant cell transformation efficiency |
| CN100370016C (zh) * | 2006-04-21 | 2008-02-20 | 贵州大学 | 对根癌农杆菌有抑制作用的黑曲霉菌株及其用途 |
| PL2121935T3 (pl) | 2006-09-25 | 2011-12-30 | Schmuelling Thomas | Transkrypcyjne represory szlaków sygnałowych cytokinin i ich zastosowanie |
| JP2011120478A (ja) * | 2008-03-31 | 2011-06-23 | Japan Tobacco Inc | アグロバクテリウム菌による形質転換植物の作成方法 |
| AU2010278126B2 (en) | 2009-07-29 | 2015-01-22 | Kaneka Corporation | A method of gene introduction into triticum plant using agrobacterium, and a method of producing transformed triticum plant |
| CA2805263A1 (en) * | 2010-07-29 | 2012-02-02 | Dow Agrosciences Llc | Strains of agrobacterium modified to increase plant transformation frequency |
| JP2013212051A (ja) | 2010-07-29 | 2013-10-17 | Japan Tobacco Inc | アグロバクテリウム菌を用いた、オオムギ属植物へ遺伝子導入を行う方法およびオオムギ属植物の形質転換植物の作成方法 |
| GB201020737D0 (en) | 2010-12-08 | 2011-01-19 | Univ Leuven Kath | Anti-oxidative stress agents |
| CA2824152C (en) * | 2011-01-17 | 2022-12-13 | Philip Morris Products S.A. | Method and system for producing a heterologous polypeptide in a nicotiana tabacum plant |
| DE102013020605A1 (de) | 2013-12-15 | 2015-06-18 | Kws Saat Ag | Selektionsmarker-freies rhizobiaceae-vermitteltes verfahren zur herstellung einer transgenen pflanze der gattung triticum |
| BR102014023930A2 (pt) | 2014-09-26 | 2016-05-17 | Ctc Ct De Tecnologia Canavieira S A | método para transformar uma célula de planta ou tecido de planta usando agrobacterium, planta transgênica, célula transgênica ou tecido transgênico, meio de cultura, e, uso de um método para transformar uma célula de planta ou tecido de planta |
| CN116577307B (zh) * | 2023-05-16 | 2025-09-09 | 重庆极泽生物科技有限公司 | 一种基于浊度检测的禽胆汁固体物质的测量方法 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5380831A (en) | 1986-04-04 | 1995-01-10 | Mycogen Plant Science, Inc. | Synthetic insecticidal crystal protein gene |
| US5610042A (en) | 1991-10-07 | 1997-03-11 | Ciba-Geigy Corporation | Methods for stable transformation of wheat |
-
1998
- 1998-02-18 MX MX9911116A patent/MX257801B/es active IP Right Grant
- 1998-05-29 CN CNB988056291A patent/CN1150320C/zh not_active Expired - Lifetime
- 1998-05-29 BR BRPI9816263A patent/BRPI9816263B1/pt not_active IP Right Cessation
- 1998-05-29 KR KR19997011305A patent/KR100505910B1/ko not_active Expired - Fee Related
- 1998-05-29 UA UA99126545A patent/UA72443C2/uk unknown
- 1998-05-29 BR BRPI9809899A patent/BRPI9809899B8/pt not_active IP Right Cessation
- 1998-05-29 AT AT98936297T patent/ATE336890T1/de not_active IP Right Cessation
- 1998-05-29 AR ARP980102541A patent/AR015837A1/es active IP Right Grant
- 1998-05-29 HU HU0002903A patent/HU226212B1/hu not_active IP Right Cessation
- 1998-05-29 IL IL13276898A patent/IL132768A0/xx active IP Right Grant
- 1998-05-29 AU AU85355/98A patent/AU735472B2/en not_active Expired
- 1998-05-29 TR TR1999/02941T patent/TR199902941T2/xx unknown
- 1998-05-29 EP EP98936297A patent/EP0986299B1/en not_active Expired - Lifetime
- 1998-05-29 CA CA002290863A patent/CA2290863C/en not_active Expired - Lifetime
- 1998-05-29 JP JP50145199A patent/JP2002502252A/ja not_active Ceased
- 1998-05-29 RU RU2000100039/13A patent/RU2226549C2/ru active
- 1998-05-29 PL PL98336979A patent/PL336979A1/xx not_active IP Right Cessation
- 1998-05-29 WO PCT/EP1998/003215 patent/WO1998054961A2/en not_active Ceased
- 1998-05-29 DE DE69835672T patent/DE69835672T2/de not_active Expired - Lifetime
- 1998-05-29 TR TR2004/02566T patent/TR200402566T2/xx unknown
- 1998-06-01 ZA ZA984681A patent/ZA984681B/xx unknown
-
1999
- 1999-11-04 IL IL132768A patent/IL132768A/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| DE69835672D1 (de) | 2006-10-05 |
| TR199902941T2 (xx) | 2000-08-21 |
| AU735472B2 (en) | 2001-07-12 |
| EP0986299B1 (en) | 2006-08-23 |
| BR9809899B1 (pt) | 2013-03-19 |
| PL336979A1 (en) | 2000-07-31 |
| DE69835672T2 (de) | 2007-08-16 |
| CA2290863A1 (en) | 1998-12-10 |
| HU226212B1 (en) | 2008-06-30 |
| ZA984681B (en) | 1998-12-02 |
| WO1998054961A2 (en) | 1998-12-10 |
| IL132768A (en) | 2007-06-17 |
| CN1258316A (zh) | 2000-06-28 |
| IL132768A0 (en) | 2001-03-19 |
| AU8535598A (en) | 1998-12-21 |
| BRPI9809899B8 (pt) | 2017-03-07 |
| MX9911116A (en) | 2000-03-31 |
| KR20010013310A (ko) | 2001-02-26 |
| ATE336890T1 (de) | 2006-09-15 |
| JP2002502252A (ja) | 2002-01-22 |
| CA2290863C (en) | 2008-09-23 |
| UA72443C2 (uk) | 2005-03-15 |
| MX257801B (en) | 2008-06-10 |
| KR100505910B1 (ko) | 2005-08-04 |
| CN1150320C (zh) | 2004-05-19 |
| AR015837A1 (es) | 2001-05-30 |
| WO1998054961A3 (en) | 1999-03-04 |
| TR200402566T2 (tr) | 2004-11-22 |
| BR9809899A (pt) | 2000-10-17 |
| EP0986299A2 (en) | 2000-03-22 |
| HUP0002903A3 (en) | 2002-08-28 |
| HUP0002903A2 (hu) | 2000-12-28 |
| RU2226549C2 (ru) | 2004-04-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US6162965A (en) | Plant transformation methods | |
| BRPI9816263B1 (pt) | método de transformação de uma célula vegetal com um gene de interesse e de preparação de uma planta transgênica fértil | |
| Cho et al. | High-efficiency induction of soybean hairy roots and propagation of the soybean cyst nematode | |
| May et al. | Generation of transgenic banana (Musa acuminata) plants via Agrobacterium-mediated transformation | |
| Chowrira et al. | Transgenic grain legumes obtained by in planta electroporation-mediated gene transfer | |
| DE69721840T2 (de) | Zuckerrohr bacilliformviruspromotor | |
| Scorza et al. | Agrobacterium-mediated transformation of peach (Prunus persica L. Batsch) leaf segments, immature embryos, and long-term embryogenic callus | |
| Song et al. | Stable and efficient agrobacterium-mediated genetic transformation of larch using embryogenic callus | |
| KR20080093100A (ko) | 초기 배아 발달 동안 성장 및/또는 발달 관련 유전자의유전자전이 과발현을 통해 증가된 종자 크기 및 종자 수 | |
| Tyagi et al. | Regeneration and Agrobacterium-mediated transformation of a popular indica rice variety, ADT39 | |
| Furini et al. | Agrobacterium-mediated transformation of the desiccation-tolerant plant Craterostigma plantagineum | |
| Tor et al. | Isolation and culture of protoplasts from immature leaves and embryogenic cell suspensions of Dioscorea yams: tools for transient gene expression studies | |
| Zhu et al. | Enhancing disease resistances of super hybrid rice with four antifungal genes | |
| Batra et al. | Agrobacterium-mediated transient GUS gene expression in buffel grass (Cenchrus ciliaris L.) | |
| Hu et al. | Shoot Regeneration from Cultured Leaf Explants of Lycium barbarumand Agrobacterium-Mediated Transformation1 | |
| CN118048364A (zh) | GhTKPR1_8基因在调控植物雄性不育中的应用 | |
| Li et al. | Expression of foreign genes, GUS and hygromycin resistance, in the halophyte Kosteletzkya virginica in response to bombardment with the Particle Inflow Gun | |
| US20060075525A1 (en) | Transgenic Echinacea plants | |
| US20020088029A1 (en) | Plant transformation methods | |
| Ramli et al. | Development of a transient promoter assay system for oil palm | |
| Sefasi et al. | Transient expression of β-glucuronidase in recalcitrant ugandan sweetpotato and putative transformation with two cry genes | |
| Tang et al. | Development of transgenic rice pure lines with enhanced resistance to rice brown planthopper | |
| ES2353370T3 (es) | Promotor de sintasa mip del maíz. | |
| MX2013014098A (es) | Composiciones, sistemas, metodos, microorgnismos, y plantas de transformacion de patata. | |
| Ozcan | Tissue culture in pea and engineering a marker gene for specific expression in target cells for plant transformation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B11E | Dismissal acc. art. 34 of ipl - requirements for examination incomplete | ||
| B11N | Dismissal: publication cancelled [chapter 11.14 patent gazette] |
Free format text: REF. A PUBL. 11.5 NA RPI NO 2151 DE 27/03/2012. |
|
| B09B | Patent application refused [chapter 9.2 patent gazette] |
Free format text: INDEFIRO O PEDIDO DE ACORDO COM O(S) ARTIGO(S) 22 DA LPI. |
|
| B12B | Appeal against refusal [chapter 12.2 patent gazette] | ||
| B16A | Patent or certificate of addition of invention granted [chapter 16.1 patent gazette] |
Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 16/08/2016, OBSERVADAS AS CONDICOES LEGAIS. |
|
| B21F | Lapse acc. art. 78, item iv - on non-payment of the annual fees in time | ||
| B21H | Decision of lapse of a patent or of a certificate of addition of invention cancelled [chapter 21.8 patent gazette] |