BRPI1009752A2 - Sistema de vetor de expressão comprendendo dois marcadores de seleção - Google Patents
Sistema de vetor de expressão comprendendo dois marcadores de seleçãoInfo
- Publication number
- BRPI1009752A2 BRPI1009752A2 BRPI1009752-0A BRPI1009752A BRPI1009752A2 BR PI1009752 A2 BRPI1009752 A2 BR PI1009752A2 BR PI1009752 A BRPI1009752 A BR PI1009752A BR PI1009752 A2 BRPI1009752 A2 BR PI1009752A2
- Authority
- BR
- Brazil
- Prior art keywords
- expression vector
- vector system
- selection markers
- markers
- selection
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
- C12N9/0028—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1)
- C12N9/003—Dihydrofolate reductase [DHFR] (1.5.1.3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y105/00—Oxidoreductases acting on the CH-NH group of donors (1.5)
- C12Y105/01—Oxidoreductases acting on the CH-NH group of donors (1.5) with NAD+ or NADP+ as acceptor (1.5.1)
- C12Y105/01003—Dihydrofolate reductase (1.5.1.3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/40—Systems of functionally co-operating vectors
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP09153995 | 2009-02-27 | ||
| EP09153995.7 | 2009-02-27 | ||
| PCT/EP2010/001224 WO2010097240A1 (en) | 2009-02-27 | 2010-02-26 | Expression vector system comprising two selection markers |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| BRPI1009752A2 true BRPI1009752A2 (pt) | 2015-08-25 |
| BRPI1009752B1 BRPI1009752B1 (pt) | 2020-03-10 |
Family
ID=40886421
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI1009752-0A BRPI1009752B1 (pt) | 2009-02-27 | 2010-02-26 | Vetor de expressão ou uma combinação de pelo menos dois vetores de expressão e métodos para sua produção e sua seleção, bem como processo para produzir um produto de interesse e uso de um meio de cultura seletivo |
Country Status (16)
| Country | Link |
|---|---|
| US (3) | US9534226B2 (pt) |
| EP (1) | EP2401377B1 (pt) |
| JP (2) | JP5801207B2 (pt) |
| KR (1) | KR101707251B1 (pt) |
| CN (1) | CN102333871B (pt) |
| BR (1) | BRPI1009752B1 (pt) |
| CA (1) | CA2753814C (pt) |
| DK (1) | DK2401377T3 (pt) |
| ES (1) | ES2587630T3 (pt) |
| IL (1) | IL214621A (pt) |
| MX (1) | MX2011009025A (pt) |
| PL (1) | PL2401377T3 (pt) |
| PT (1) | PT2401377T (pt) |
| RU (2) | RU2535986C2 (pt) |
| SG (1) | SG173456A1 (pt) |
| WO (1) | WO2010097240A1 (pt) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102333871B (zh) | 2009-02-27 | 2013-12-04 | 诺瓦提斯公司 | 包含两个选择标记的表达载体系统 |
| US20130244231A1 (en) * | 2010-11-08 | 2013-09-19 | Jcr Pharmaceuticals Co., Ltd. | Novel expression vector |
| KR102286389B1 (ko) | 2012-11-20 | 2021-08-05 | 노파르티스 아게 | 폴리펩티드를 고 수율로 발현하는 최적화 발현 카세트 |
| US20160017319A1 (en) | 2013-03-11 | 2016-01-21 | Audrey Nommay | Method of screening cell clones |
| EP3027646B1 (en) * | 2013-07-31 | 2018-06-27 | Novartis AG | Novel selection vectors and methods of selecting eukaryotic host cells |
| EP3083677B1 (en) | 2013-12-20 | 2019-08-28 | Novartis AG | Novel eukaryotic cells and methods for recombinantly expressing a product of interest |
| US11203631B2 (en) | 2013-12-20 | 2021-12-21 | Novartis Ag | Eukaryotic cells and methods for recombinantly expressing a product of interest |
| US11512335B2 (en) | 2014-04-29 | 2022-11-29 | Novartis Ag | Vertebrate cells and methods for recombinantly expressing a polypeptide of interest |
| JP6767991B2 (ja) * | 2015-04-03 | 2020-10-14 | セレクシス エス．エー． | 哺乳動物細胞における組換えタンパク質生産のためのビタミンならびにビタミン代謝関連の遺伝子およびタンパク質の使用 |
| UY37758A (es) | 2017-06-12 | 2019-01-31 | Novartis Ag | Método de fabricación de anticuerpos biespecíficos, anticuerpos biespecíficos y uso terapéutico de dichos anticuerpos |
| JP2020536513A (ja) | 2017-10-02 | 2020-12-17 | アストラゼネカ・アクチエボラーグＡｓｔｒａｚｅｎｅｃａ Ａｋｔｉｅｂｏｌａｇ | タンパク質産生の増加のための細胞株及び方法 |
| CN114401769A (zh) * | 2019-03-27 | 2022-04-26 | 菲尼克斯组织修复公司 | 生产胶原蛋白7组合物的系统和方法 |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3438889B2 (ja) | 1991-03-29 | 2003-08-18 | ジェネンテク，インコーポレイテッド | 所望のタンパク質を高レベルに発現させる組換え宿主細胞の選別法 |
| US5439808A (en) | 1993-07-23 | 1995-08-08 | North American Vaccine, Inc. | Method for the high level expression, purification and refolding of the outer membrane group B porin proteins from Neisseria meningitidis |
| US5830698A (en) * | 1997-03-14 | 1998-11-03 | Idec Pharmaceuticals Corporation | Method for integrating genes at specific sites in mammalian cells via homologous recombination and vectors for accomplishing the same |
| ATE258131T1 (de) * | 2000-04-27 | 2004-02-15 | Olsen Design Aps | Verstäuung mit ummantelung zur verminderung der verschmutzung eines schiffes |
| DE10036491A1 (de) * | 2000-07-25 | 2002-02-07 | Roche Diagnostics Gmbh | Expression von Alkalischer Phosphatase in Hefe |
| US7384744B2 (en) * | 2002-11-29 | 2008-06-10 | Boehringer Ingelheim Pharma Gmbh & Co., Kg | Expression vector, methods for the production of heterologous gene products and for the selection of recombinant cells producing high levels of such products |
| DE10256083A1 (de) * | 2002-11-29 | 2004-08-12 | Boehringer Ingelheim Pharma Gmbh & Co. Kg | Expressionsvektor, Verfahren zur Herstellung von heterologen Genprodukten und Selektionsverfahren für hochproduzierende rekombinante Zellen |
| KR101180139B1 (ko) | 2003-03-11 | 2012-09-13 | 메르크 세로노 에스.에이. | Ｍｃｍｖ ｉｅ2 프로모터를 포함하는 발현 벡터 |
| TW200513351A (en) * | 2003-07-17 | 2005-04-16 | Availvs Corp | High pressure water jet surface cutting device and cutting method |
| DK1716233T3 (da) | 2004-01-30 | 2009-10-26 | Maxygen Holdings Ltd | Reguleret stopkodongennemlæsning |
| IL165484A0 (en) * | 2004-11-30 | 2006-01-15 | Applied Research Systems | Production of proteins |
| ATE511544T1 (de) * | 2006-02-21 | 2011-06-15 | Chromagenics Bv | Selektion von wirtszellen mit proteinexpression auf hohem niveau |
| EP2208792B1 (en) | 2006-05-17 | 2015-05-06 | Hoffmann-La Roche AG | Polypeptide producing cells |
| PT2227549E (pt) | 2007-12-21 | 2015-10-08 | Novartis Ag | Sistema de seleção para cultura de células eucarióticas com base num gene de recetor de folato ligado à membrana |
| KR101734585B1 (ko) | 2008-08-28 | 2017-05-11 | 노파르티스 아게 | 정지 코돈 번역초과에 의한 폴리펩티드 이소형의 세포 표면 표시 |
| DK2401383T3 (da) | 2009-02-27 | 2013-12-16 | Novartis Ag | Fremgangsmåder til udvælgelse af eukaryote celler, som udtrykker et heterologt protein |
| CN102333871B (zh) | 2009-02-27 | 2013-12-04 | 诺瓦提斯公司 | 包含两个选择标记的表达载体系统 |
| EP3027646B1 (en) | 2013-07-31 | 2018-06-27 | Novartis AG | Novel selection vectors and methods of selecting eukaryotic host cells |
-
2010
- 2010-02-26 CN CN2010800095054A patent/CN102333871B/zh active Active
- 2010-02-26 PL PL10711001.7T patent/PL2401377T3/pl unknown
- 2010-02-26 WO PCT/EP2010/001224 patent/WO2010097240A1/en active Application Filing
- 2010-02-26 PT PT107110017T patent/PT2401377T/pt unknown
- 2010-02-26 RU RU2011139182/10A patent/RU2535986C2/ru active
- 2010-02-26 DK DK10711001.7T patent/DK2401377T3/en active
- 2010-02-26 SG SG2011054079A patent/SG173456A1/en unknown
- 2010-02-26 KR KR1020117022541A patent/KR101707251B1/ko active IP Right Grant
- 2010-02-26 EP EP10711001.7A patent/EP2401377B1/en active Active
- 2010-02-26 CA CA2753814A patent/CA2753814C/en active Active
- 2010-02-26 JP JP2011551444A patent/JP5801207B2/ja active Active
- 2010-02-26 US US13/203,610 patent/US9534226B2/en active Active
- 2010-02-26 ES ES10711001.7T patent/ES2587630T3/es active Active
- 2010-02-26 BR BRPI1009752-0A patent/BRPI1009752B1/pt active IP Right Grant
- 2010-02-26 MX MX2011009025A patent/MX2011009025A/es active IP Right Grant
-
2011
- 2011-08-11 IL IL214621A patent/IL214621A/en active IP Right Grant
-
2014
- 2014-01-21 RU RU2014101759A patent/RU2649361C2/ru active
- 2014-12-17 JP JP2014254954A patent/JP2015109839A/ja not_active Withdrawn
-
2016
- 2016-11-21 US US15/356,748 patent/US9994866B2/en active Active
-
2018
- 2018-05-24 US US15/988,141 patent/US11174494B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| RU2014101759A (ru) | 2015-08-10 |
| JP2015109839A (ja) | 2015-06-18 |
| CA2753814C (en) | 2018-07-31 |
| PT2401377T (pt) | 2016-08-18 |
| WO2010097240A1 (en) | 2010-09-02 |
| US20110306092A1 (en) | 2011-12-15 |
| MX2011009025A (es) | 2011-09-28 |
| US9994866B2 (en) | 2018-06-12 |
| PL2401377T3 (pl) | 2016-11-30 |
| DK2401377T3 (en) | 2016-08-29 |
| SG173456A1 (en) | 2011-09-29 |
| CN102333871B (zh) | 2013-12-04 |
| US9534226B2 (en) | 2017-01-03 |
| RU2535986C2 (ru) | 2014-12-20 |
| US20180265891A1 (en) | 2018-09-20 |
| EP2401377A1 (en) | 2012-01-04 |
| JP5801207B2 (ja) | 2015-10-28 |
| IL214621A0 (en) | 2011-09-27 |
| KR20110119837A (ko) | 2011-11-02 |
| CA2753814A1 (en) | 2010-09-02 |
| JP2012518992A (ja) | 2012-08-23 |
| AU2010219089A1 (en) | 2011-08-18 |
| CN102333871A (zh) | 2012-01-25 |
| KR101707251B1 (ko) | 2017-02-15 |
| RU2649361C2 (ru) | 2018-04-02 |
| EP2401377B1 (en) | 2016-05-18 |
| BRPI1009752B1 (pt) | 2020-03-10 |
| US11174494B2 (en) | 2021-11-16 |
| ES2587630T3 (es) | 2016-10-25 |
| US20170067077A1 (en) | 2017-03-09 |
| IL214621A (en) | 2016-02-29 |
| RU2011139182A (ru) | 2013-04-10 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
| B06A | Patent application procedure suspended [chapter 6.1 patent gazette] | ||
| B06A | Patent application procedure suspended [chapter 6.1 patent gazette] | ||
| B09A | Decision: intention to grant [chapter 9.1 patent gazette] | ||
| B16A | Patent or certificate of addition of invention granted [chapter 16.1 patent gazette] |
Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 10/03/2020, OBSERVADAS AS CONDICOES LEGAIS. |