BRPI0818787A2 - Composição secada para pcr de partida quente com estabilidade de longa duração. - Google Patents

Composição secada para pcr de partida quente com estabilidade de longa duração.

Info

Publication number
BRPI0818787A2
BRPI0818787A2 BRPI0818787A BRPI0818787A2 BR PI0818787 A2 BRPI0818787 A2 BR PI0818787A2 BR PI0818787 A BRPI0818787 A BR PI0818787A BR PI0818787 A2 BRPI0818787 A2 BR PI0818787A2
Authority
BR
Brazil
Prior art keywords
long lasting
dried composition
lasting stability
hot starting
starting pcr
Prior art date
Application number
Other languages
English (en)
Inventor
Seong-Youl Kim
Hyun Bae Kim
Hae-Joon Park
Han Oh Park
Original Assignee
Bioneer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bioneer Corp filed Critical Bioneer Corp
Publication of BRPI0818787A2 publication Critical patent/BRPI0818787A2/pt
Publication of BRPI0818787B1 publication Critical patent/BRPI0818787B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
BRPI0818787A 2007-10-29 2008-10-28 composição secada para pcr de partida quente com estabilidade de longa duração, método de preparação da mesma, kit para pcr de partida quente e método para ampliar o ácido nucleico BRPI0818787B1 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020070109055A KR101098764B1 (ko) 2007-10-29 2007-10-29 안정화된 핫스타트 ｐｃｒ용 건조 조성물
PCT/KR2008/006355 WO2009057931A2 (en) 2007-10-29 2008-10-28 Dried composition for hot-start pcr with long-term stability

Publications (2)

Publication Number Publication Date
BRPI0818787A2 true BRPI0818787A2 (pt) 2015-04-22
BRPI0818787B1 BRPI0818787B1 (pt) 2016-12-13

Family

ID=40591640

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0818787A BRPI0818787B1 (pt) 2007-10-29 2008-10-28 composição secada para pcr de partida quente com estabilidade de longa duração, método de preparação da mesma, kit para pcr de partida quente e método para ampliar o ácido nucleico

Country Status (5)

Country Link
US (1) US9034603B2 (pt)
KR (1) KR101098764B1 (pt)
CN (1) CN101842492A (pt)
BR (1) BRPI0818787B1 (pt)
WO (1) WO2009057931A2 (pt)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3502272A1 (en) * 2010-06-21 2019-06-26 Life Technologies Corporation Compositions, kits, and methods for synthesis and/or detection of nucleic acids
WO2013053855A1 (en) * 2011-10-11 2013-04-18 Qiagen Gmbh Sample processing method and sample processing cartridge
CN103088014A (zh) * 2011-11-08 2013-05-08 北京金菩嘉医疗科技有限公司 一种聚合酶链反应试剂及其制备方法
KR101870311B1 (ko) 2012-03-09 2018-06-25 (주)바이오니아 핫스타트 역전사반응 또는 핫스타트 역전사 중합효소 연쇄반응용 조성물
KR101545848B1 (ko) 2012-04-09 2015-08-21 (주)바이오니아 핵산중합효소로 핵산을 검출하는데 사용되는 고민감도 핵산준비방법
US9034606B2 (en) * 2012-08-23 2015-05-19 New England Biolabs, Inc. Detection of an amplification reaction product using pH-sensitive dyes
CN103849548A (zh) 2012-12-03 2014-06-11 三星电子株式会社 用于扩增核酸的试剂容器及其制备方法、存储试剂的方法和用于核酸分析的微流体系统
CN103409540B (zh) * 2013-08-19 2015-12-09 苏州吉泰生物科技有限公司 一种提高灵敏度和特异性的定量pcr方法
KR101488110B1 (ko) 2013-11-29 2015-01-29 성균관대학교산학협력단 나노아케움 이퀴탄스 유래의 Neq HS DNA 중합효소의 돌연변이체 제조 및 이를 이용한 hot-start PCR 응용
CN103849690B (zh) * 2014-01-20 2015-12-30 凯杰生物工程（深圳）有限公司 用于检测人乙型肝炎病毒的引物组和探针
US9856524B2 (en) * 2014-12-04 2018-01-02 Shaofeng Ding Lyophilized integrated composition for storage and manipulation of pyrophosphorolysis activated polymerization
US9868944B2 (en) 2014-12-19 2018-01-16 Roche Molecular Systems, Inc. Reaction mixtures
KR102021605B1 (ko) * 2015-02-13 2019-09-16 주식회사 씨젠 다중타겟 핵산서열 증폭반응용 조성물의 동결건조 방법
CN111684077A (zh) 2018-02-06 2020-09-18 简·探针公司 远红染料探针调配物
CN112384608A (zh) * 2018-05-24 2021-02-19 纽约市哥伦比亚大学理事会 细菌捕获测序平台及其设计、构建和使用方法
JP7418717B2 (ja) * 2019-06-07 2024-01-22 国立大学法人 東京医科歯科大学 微生物の検出方法
CN110628885B (zh) * 2019-10-28 2023-04-25 珠海丽珠试剂股份有限公司 反应液、pcr反应液及其使用方法和用途
KR102529958B1 (ko) * 2021-01-18 2023-05-08 가톨릭대학교 산학협력단 저 gc 함량 dna의 정량적 증폭을 위한 pcr 첨가제 조성물
KR102264902B1 (ko) * 2021-03-05 2021-06-14 주식회사 모노바이오 안정성이 향상된 pcr 프리믹스 조성물 및 이의 제조방법
WO2024063637A1 (en) * 2022-09-20 2024-03-28 Jabatan Perkhidmatan Veterinar Sabah Sybr green pcr reagents, method and kit

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5700590A (en) * 1994-01-10 1997-12-23 Abbott Laboratories Nutritional formula with ribo-nucleotides
US5556771A (en) * 1995-02-10 1996-09-17 Gen-Probe Incorporated Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification
KR100292883B1 (ko) 1999-02-09 2001-06-15 박한오 피로포스페이트와 피로포스파타제를 포함하는 핫 스타트 ｐｃｒ 반응혼합물
GB0110501D0 (en) * 2001-04-30 2001-06-20 Secr Defence Brit Amplification process
ATE555216T1 (de) * 2006-05-23 2012-05-15 Molecular Detection Inc Umgebungstemperaturstabile kits für die molekulare diagnostik

Also Published As

Publication number Publication date
US9034603B2 (en) 2015-05-19
WO2009057931A3 (en) 2009-07-23
US20100209973A1 (en) 2010-08-19
KR101098764B1 (ko) 2011-12-26
KR20090043282A (ko) 2009-05-06
CN101842492A (zh) 2010-09-22
BRPI0818787B1 (pt) 2016-12-13
WO2009057931A2 (en) 2009-05-07

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Legal Events

Date Code Title Description
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 28/10/2008, OBSERVADAS AS CONDICOES LEGAIS.