BRPI0516221A - método de produção de vìrus de uma cultura de células, composição, e, método de produção de reovìrus infeccioso - Google Patents

método de produção de vìrus de uma cultura de células, composição, e, método de produção de reovìrus infeccioso

Info

Publication number
BRPI0516221A
BRPI0516221A BRPI0516221-1A BRPI0516221A BRPI0516221A BR PI0516221 A BRPI0516221 A BR PI0516221A BR PI0516221 A BRPI0516221 A BR PI0516221A BR PI0516221 A BRPI0516221 A BR PI0516221A
Authority
BR
Brazil
Prior art keywords
virus
production
cell culture
composition
infectious
Prior art date
Application number
BRPI0516221-1A
Other languages
English (en)
Inventor
Matthew C Carey
Original Assignee
Oncolytics Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=36202651&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BRPI0516221(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Oncolytics Biotech Inc filed Critical Oncolytics Biotech Inc
Publication of BRPI0516221A publication Critical patent/BRPI0516221A/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12Antivirals
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00Antineoplastic agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/13Tumour cells, irrespective of tissue of origin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2720/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
    • C12N2720/00011Details
    • C12N2720/12011Reoviridae
    • C12N2720/12051Methods of production or purification of viral material

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Public Health (AREA)
  • Immunology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

MéTODO DE PRODUçãO DE VìRUS DE UMA CULTURA DE CéLULAS, COMPOSIçãO, E, MéTODO DE PRODUçãO DE REOVìRUS INFECCIOSO. A invenção refere-se a um método melhorado de purificação de vírus, particularmente reovírus. O vírus infeccioso pode ser extraído de uma cultura celular com um detergente para produzir altos títulos de vírus, e o vírus pode então ser purificado por etapas simples, tais como filtração e cromatografia em coluna. Os vírus e as composições que compreendem os vírus preparados de acordo com a presente invenção são também providos.
BRPI0516221-1A 2004-10-22 2005-10-21 método de produção de vìrus de uma cultura de células, composição, e, método de produção de reovìrus infeccioso BRPI0516221A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US62125704P 2004-10-22 2004-10-22
PCT/CA2005/001617 WO2006042414A1 (en) 2004-10-22 2005-10-21 Improved viral purification methods

Publications (1)

Publication Number Publication Date
BRPI0516221A true BRPI0516221A (pt) 2008-08-26

Family

ID=36202651

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0516221-1A BRPI0516221A (pt) 2004-10-22 2005-10-21 método de produção de vìrus de uma cultura de células, composição, e, método de produção de reovìrus infeccioso

Country Status (15)

Country Link
US (1) US7901921B2 (pt)
EP (1) EP1814982B1 (pt)
JP (2) JP4898690B2 (pt)
CN (2) CN105062980A (pt)
AU (1) AU2005297372B2 (pt)
BR (1) BRPI0516221A (pt)
CA (1) CA2584142C (pt)
DK (1) DK1814982T3 (pt)
ES (1) ES2562237T3 (pt)
HK (1) HK1100959A1 (pt)
IL (1) IL182562A0 (pt)
MX (1) MX2007004696A (pt)
SG (1) SG156673A1 (pt)
WO (1) WO2006042414A1 (pt)
ZA (1) ZA200704034B (pt)

Families Citing this family (60)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003093463A1 (en) * 2002-04-30 2003-11-13 Oncolytics Biotech Inc. Improved viral purification methods
US10668119B2 (en) 2005-08-01 2020-06-02 Virocure, Inc. Attenuated reovirus
EP1917351A4 (en) * 2005-08-01 2009-12-16 Univ Technologies Int ATTENUATED REOVIRUS
US20080131955A1 (en) * 2006-11-30 2008-06-05 Canon U.S. Life Sciences, Inc. Method of Separating Target DNA from Mixed DNA
US10369171B2 (en) 2007-03-13 2019-08-06 Virocure, Inc. Attenuated reoviruses for selection of cell populations
EP2240573A4 (en) * 2008-02-12 2011-08-31 Sanofi Pasteur Ltd METHOD USING ION EXCHANGE AND GELFILTRATION CHROMATOGRAPHY FOR POXVIRUS CLEANING
CN101270350A (zh) * 2008-03-05 2008-09-24 辽宁依生生物制药有限公司 一种提取狂犬病病毒的方法
US8445270B2 (en) 2009-05-12 2013-05-21 Transgene S.A. Immortalized avian cell lines and use thereof
CN102191226A (zh) * 2010-03-08 2011-09-21 北京清大天一科技有限公司 细胞培养混合物的分离纯化方法
US9644187B2 (en) 2010-04-14 2017-05-09 Emd Millipore Corporation Methods of producing high titer, high purity virus stocks and methods of use thereof
TW201233802A (en) 2010-12-02 2012-08-16 Oncolytics Biotech Inc Liquid viral formulations
DK2646052T3 (en) 2010-12-02 2017-07-17 Oncolytics Biotech Inc LYOPHILIZED VIRAL FORMULATIONS
DK2702147T3 (da) 2011-04-29 2020-09-28 Oncolytics Biotech Inc Fremgangsmåder til oprensning af vira ved hjælp af gelpermeationskromatografi
CN102628030B (zh) * 2012-01-20 2013-12-04 宁波大学 三疣梭子蟹呼肠孤病毒的鸡胚培养方法
CN103571800B (zh) * 2012-07-27 2018-04-24 江苏康润生物科技有限公司 一种去除疫苗中宿主dna的方法
US10577627B2 (en) 2014-06-09 2020-03-03 Voyager Therapeutics, Inc. Chimeric capsids
EP3172317B1 (en) * 2014-07-24 2019-05-01 Janssen Vaccines & Prevention B.V. Process for the purification of poliovirus from cell cultures
MX2017005834A (es) 2014-11-05 2017-11-17 Voyager Therapeutics Inc Polinucleotidos aad para el tratamiento de la enfermedad de parkinson.
EP3218484A4 (en) 2014-11-14 2018-05-30 Voyager Therapeutics, Inc. Compositions and methods of treating amyotrophic lateral sclerosis (als)
RU2749882C2 (ru) 2014-11-14 2021-06-18 Вояджер Терапьютикс, Инк. Модулирующие полинуклеотиды
US11697825B2 (en) 2014-12-12 2023-07-11 Voyager Therapeutics, Inc. Compositions and methods for the production of scAAV
EP3054007A1 (en) 2015-02-09 2016-08-10 Institut National De La Sante Et De La Recherche Medicale (Inserm) Recombinant adeno-associated virus particle purification comprising an immuno-affinity purification step
WO2016201224A1 (en) * 2015-06-10 2016-12-15 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Processes for production and purification of nucleic acid-containing compositions
US20180230489A1 (en) 2015-10-28 2018-08-16 Voyager Therapeutics, Inc. Regulatable expression using adeno-associated virus (aav)
EP3448874A4 (en) 2016-04-29 2020-04-22 Voyager Therapeutics, Inc. COMPOSITIONS FOR TREATING A DISEASE
US11299751B2 (en) 2016-04-29 2022-04-12 Voyager Therapeutics, Inc. Compositions for the treatment of disease
JP7220080B2 (ja) 2016-05-18 2023-02-09 ボイジャー セラピューティクス インコーポレイテッド ハンチントン病治療組成物及び方法
RU2758488C2 (ru) 2016-05-18 2021-10-28 Вояджер Терапьютикс, Инк. Модулирующие полинуклеотиды
EP3506817A4 (en) 2016-08-30 2020-07-22 The Regents of The University of California METHOD FOR BIOMEDICAL TARGETING AND RELEASE, AND DEVICES AND SYSTEMS FOR IMPLEMENTING THEM
WO2018204786A1 (en) 2017-05-05 2018-11-08 Voyager Therapeutics, Inc. Compositions and methods of treating amyotrophic lateral sclerosis (als)
WO2018204803A1 (en) 2017-05-05 2018-11-08 Voyager Therapeutics, Inc. Compositions and methods of treating huntington's disease
JOP20190269A1 (ar) 2017-06-15 2019-11-20 Voyager Therapeutics Inc بولي نوكليوتيدات aadc لعلاج مرض باركنسون
US10851350B1 (en) * 2017-06-27 2020-12-01 Genelux Corporation Bioreactor production of virus from adherent cells
JP7229989B2 (ja) 2017-07-17 2023-02-28 ボイジャー セラピューティクス インコーポレイテッド 軌道アレイガイドシステム
EP3662060A2 (en) 2017-08-03 2020-06-10 Voyager Therapeutics, Inc. Compositions and methods for delivery of aav
EP3697908A1 (en) 2017-10-16 2020-08-26 Voyager Therapeutics, Inc. Treatment of amyotrophic lateral sclerosis (als)
EP4124658A3 (en) 2017-10-16 2023-04-19 Voyager Therapeutics, Inc. Treatment of amyotrophic lateral sclerosis (als)
US11603527B2 (en) * 2017-12-27 2023-03-14 Global Life Sciences Solutions Usa Llc Method and kit for viral vector isolation
EP3762500A1 (en) 2018-03-06 2021-01-13 Voyager Therapeutics, Inc. Insect cell manufactured partial self-complementary aav genomes
WO2019222441A1 (en) 2018-05-16 2019-11-21 Voyager Therapeutics, Inc. Aav serotypes for brain specific payload delivery
WO2019222444A2 (en) 2018-05-16 2019-11-21 Voyager Therapeutics, Inc. Directed evolution
US11690907B2 (en) 2018-06-12 2023-07-04 Kbio Holdings Limited Vaccines formed by virus and antigen conjugation
US11529413B2 (en) 2018-06-12 2022-12-20 Kbio Holdings Limited Virus and antigen purification and conjugation
CN112566494B (zh) 2018-06-12 2023-05-23 Kbio控股有限公司 病毒和抗原纯化和偶联
US11696948B2 (en) 2018-06-12 2023-07-11 Kbio Holdings Limited Vaccines formed by virus and antigen conjugation
JP7565218B2 (ja) 2018-07-02 2024-10-10 ボイジャー セラピューティクス インコーポレイテッド 筋萎縮性側索硬化症および脊髄に関連する障害の治療
US11999975B2 (en) * 2018-07-04 2024-06-04 Probiogen Ag Method for purifying an enveloped virus
US20210355454A1 (en) 2018-07-24 2021-11-18 Voyager Therapeutics, Inc. Systems and methods for producing gene therapy formulations
US20210348242A1 (en) 2018-10-04 2021-11-11 Voyager Therapeutics, Inc. Methods for measuring the titer and potency of viral vector particles
US20220064671A1 (en) 2019-01-18 2022-03-03 Voyager Therapeutics, Inc. Methods and systems for producing aav particles
KR102228267B1 (ko) * 2019-01-25 2021-03-17 바이로큐어 주식회사 Bhk-21 세포를 이용한 바이러스 생산방법
EP3962536A1 (en) 2019-04-29 2022-03-09 Voyager Therapeutics, Inc. Systems and methods for producing baculoviral infected insect cells (biics) in bioreactors
EP4010465A1 (en) 2019-08-09 2022-06-15 Voyager Therapeutics, Inc. Cell culture medium for use in producing gene therapy products in bioreactors
US20220364114A1 (en) 2019-08-26 2022-11-17 Voyager Therapeutics, Inc. Controlled expression of viral proteins
WO2022032153A1 (en) 2020-08-06 2022-02-10 Voyager Therapeutics, Inc. Cell culture medium for use in producing gene therapy products in bioreactors
WO2022187473A2 (en) 2021-03-03 2022-09-09 Voyager Therapeutics, Inc. Controlled expression of viral proteins
US20240141378A1 (en) 2021-03-03 2024-05-02 Voyager Therapeutics, Inc. Controlled expression of viral proteins
CN113913397A (zh) * 2021-11-23 2022-01-11 贵州医科大学 一种溶瘤病毒的纯化方法
CN115300621B (zh) * 2022-08-05 2023-12-12 上海生物制品研究所有限责任公司 水痘-带状疱疹减毒活疫苗制备方法和系统
WO2024054983A1 (en) 2022-09-08 2024-03-14 Voyager Therapeutics, Inc. Controlled expression of viral proteins

Family Cites Families (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB896439A (en) 1959-12-28 1962-05-16 Glaxo Lab Ltd Cellulose derivatives useful as anion-exchange materials and their application
DE1182391B (de) 1961-10-14 1964-11-26 Behringwerke Ag Verfahren zur Desaggregation und Reinigung von Viren
US4070453A (en) 1976-06-25 1978-01-24 Syntex (U.S.A.) Inc. Diploid porcine embryonic cell strains, cultures produced therefrom, and use of said cultures for production of vaccines
US4721675A (en) 1982-02-01 1988-01-26 Abbott Laboratories Production of hepatitis A virus in vitro utilizing a persistently virus infected cell culture system
US4559229A (en) 1982-07-20 1985-12-17 Research Foundation Avian proventriculitis vaccine
US5023252A (en) 1985-12-04 1991-06-11 Conrex Pharmaceutical Corporation Transdermal and trans-membrane delivery of drugs
JPS6344532A (ja) 1986-08-11 1988-02-25 Biseibutsu Kagaku Kenkyusho:Kk 豚のレオウイルス感染症ワクチン
US5948441A (en) 1988-03-07 1999-09-07 The Liposome Company, Inc. Method for size separation of particles
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
IT1248075B (it) * 1991-06-18 1995-01-05 Sclavo Spa Processo per la purificazione del virus dell'epatite a (hav), virus cosi` purificato e composizioni vaccinali che lo contengono.
US5658779A (en) 1992-03-20 1997-08-19 Ligochem, Inc. Method of adsorbing viruses from fluid compositions
DZ1706A1 (fr) 1992-08-07 2002-02-17 Merck & Co Inc Vaccin contre le virus de l'hepatite a.
FR2696748B1 (fr) * 1992-10-14 1994-12-30 Pasteur Merieux Serums Vacc Procédé de préparation d'antigènes et de vaccins de l'hépatite A (HAV).
US5614413A (en) 1993-07-01 1997-03-25 The Uab Research Foundation Encapsidated recombinant poliovirus nucleic acid and methods of making and using same
US6686200B1 (en) * 1993-08-31 2004-02-03 Uab Research Foundation Methods and compositions for the large scale production of recombinant adeno-associated virus
HRP950097A2 (en) 1994-03-08 1997-06-30 Merck & Co Inc Hepatitis a virus culture process
CN1152935A (zh) 1994-05-16 1997-06-25 麦克公司 乳头状瘤病毒疫苗
US6146873A (en) 1994-11-10 2000-11-14 Baxter Aktiengesellschaft Production of orthomyxoviruses in monkey kidney cells using protein-free media
AR004464A1 (es) 1994-11-14 1998-12-16 Merck Sharp & Dohme Un metodo para producir una proteina de capside de papilomavirus
WO1997008298A1 (en) * 1995-08-30 1997-03-06 Genzyme Corporation Chromatographic purification of adenovirus and aav
RU2194531C2 (ru) * 1996-07-02 2002-12-20 Коннот Лабораториз Лимитед Поливалентные ассоциированные коклюшно-дифтерийно-столбнячно (акдс)-полиомиелитные вакцины
ATE550429T1 (de) 1996-11-20 2012-04-15 Crucell Holland Bv Adenovirus-zusammensetzungen erhältlich durch ein verbessertes produktions- und reinigungsverfahren
IL130198A (en) 1996-12-13 2005-09-25 Schering Corp Methods for purifying viruses
TW570803B (en) 1997-04-09 2004-01-11 Duphar Int Res Influenza vaccine
US6214333B1 (en) 1997-07-08 2001-04-10 Texas Heart Institute Vasoprotective recombinant adenovirus vector containing a human TFPI gene
US6110461A (en) 1997-08-13 2000-08-29 Oncolytics Biotech Inc. Reovirus for the treatment of neoplasia
US6136307A (en) 1997-08-13 2000-10-24 Oncolytics Biotech Inc. Reovirus for the treatment of cellular proliferative disorders
DE19812940A1 (de) * 1998-03-24 1999-10-07 Medigene Ag Formulierung mit Papillomavirus-spezifischem Protein, seine Herstellung und Verwendung
CA2356373A1 (fr) * 1998-12-31 2000-07-13 Aventis Pharma S.A. Methode de separation de particules virales
US6376210B1 (en) * 1999-07-06 2002-04-23 General Atomics Methods and compositions for assaying analytes
CN1139656C (zh) * 1999-11-17 2004-02-25 本元正阳基因技术股份有限公司 一种快速高效分离和纯化重组腺病毒相关病毒的方法和用途
US20040241176A1 (en) * 2000-04-27 2004-12-02 Ap Cells. Inc. Method of producing membrane vesicles
AU2001275191A1 (en) 2000-05-31 2001-12-11 Chiron Corporation Method for the purification of alphavirus replicon particles
TWI289158B (en) 2000-08-10 2007-11-01 Oncolytics Biotech Inc Method of producing infectious reovirus
CA2427029C (en) * 2000-10-27 2010-02-02 F. Hoffmann-La Roche Ag Variants of soluble pyrroloquinoline quinone-dependent glucose dehydrogenase
EP1370643A1 (en) 2001-03-16 2003-12-17 Oncolytics Biotech, Inc. Method of extracting virus from cell culture
WO2003093463A1 (en) * 2002-04-30 2003-11-13 Oncolytics Biotech Inc. Improved viral purification methods
JP4268384B2 (ja) * 2002-08-07 2009-05-27 財団法人阪大微生物病研究会 日本脳炎ウイルス抗原及びその製造方法

Also Published As

Publication number Publication date
EP1814982A4 (en) 2009-03-04
WO2006042414A1 (en) 2006-04-27
ES2562237T3 (es) 2016-03-03
US7901921B2 (en) 2011-03-08
IL182562A0 (en) 2007-07-24
JP2008516616A (ja) 2008-05-22
MX2007004696A (es) 2007-06-14
CN105062980A (zh) 2015-11-18
JP4898690B2 (ja) 2012-03-21
SG156673A1 (en) 2009-11-26
EP1814982A1 (en) 2007-08-08
CA2584142A1 (en) 2006-04-27
US20060088869A1 (en) 2006-04-27
DK1814982T3 (en) 2016-03-21
ZA200704034B (en) 2008-08-27
HK1100959A1 (en) 2007-10-05
CA2584142C (en) 2011-11-29
EP1814982B1 (en) 2015-12-23
AU2005297372B2 (en) 2010-08-26
JP2012040026A (ja) 2012-03-01
AU2005297372A1 (en) 2006-04-27
CN101098959A (zh) 2008-01-02

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B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

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