BRPI0513998A - detoxifizima com atividade de transformar aflotoxina e codificação do gene da mesma - Google Patents

detoxifizima com atividade de transformar aflotoxina e codificação do gene da mesma

Info

Publication number
BRPI0513998A
BRPI0513998A BRPI0513998-8A BRPI0513998A BRPI0513998A BR PI0513998 A BRPI0513998 A BR PI0513998A BR PI0513998 A BRPI0513998 A BR PI0513998A BR PI0513998 A BRPI0513998 A BR PI0513998A
Authority
BR
Brazil
Prior art keywords
transforming
detoxifizyme
adtz
aflatoxin
activity
Prior art date
Application number
BRPI0513998-8A
Other languages
English (en)
Inventor
Dongsheng Yao
Daling Liu
Min Guan
Chunfang Xie
Original Assignee
Guangzhou Co Win Bioengineerin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guangzhou Co Win Bioengineerin filed Critical Guangzhou Co Win Bioengineerin
Publication of BRPI0513998A publication Critical patent/BRPI0513998A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • A61P39/02—Antidotes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Physics & Mathematics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Epidemiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Fodder In General (AREA)

Abstract

DETOXIFIZIMA COM ATIVIDADE DE TRANSFORMAR AFLOTOXINA E CODIFICAçãO DO GENE Dá MESMA A presente invenção se relaciona a uma detoxifizima com a ação de transformar aflatoxina e os genes codificados dela. Os inventores isolam primeiramente e purificam uma proteína nova, nomeada aflatoxina-detoxifizima (ADTZ) que tem a atividade de transformar aflatoxina. As bases específicas dos genes ADTZ são obtidas através da purificação e seqüenciamento. O gene codificado de ADTZ é danado de todo RNA da Armillariella tabescens. A proteína recombinante é expressa e purificada por vários sistemas de manifestação que usam métodos de engenharia genética. A detoxifizima dita tem bioatividade de transformar AFB~ 1~, reduzindo efeitos mutagênicos de AFB~ 1~. Tem grande potencial para fabricar alimentação ou alimento e de desenvolver medicamento antitumor.
BRPI0513998-8A 2004-08-17 2005-01-13 detoxifizima com atividade de transformar aflotoxina e codificação do gene da mesma BRPI0513998A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CNB2004100511200A CN1294255C (zh) 2004-08-17 2004-08-17 一种具有转化黄曲霉毒素活性的解毒酶及编码该酶的基因
PCT/CN2005/000050 WO2006017960A1 (fr) 2004-08-17 2005-01-13 Enzyme de détoxification ayant une activité consistant à transformer l'aflatoxine et gène codant pour celle-ci

Publications (1)

Publication Number Publication Date
BRPI0513998A true BRPI0513998A (pt) 2008-05-20

Family

ID=35718321

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0513998-8A BRPI0513998A (pt) 2004-08-17 2005-01-13 detoxifizima com atividade de transformar aflotoxina e codificação do gene da mesma

Country Status (16)

Country Link
US (1) US7695751B2 (pt)
EP (1) EP1780270B1 (pt)
JP (1) JP4495758B2 (pt)
KR (1) KR100850640B1 (pt)
CN (1) CN1294255C (pt)
AT (1) ATE489458T1 (pt)
AU (1) AU2005274578B2 (pt)
BR (1) BRPI0513998A (pt)
CA (1) CA2576069C (pt)
DE (1) DE602005024973D1 (pt)
HK (1) HK1106272A1 (pt)
IL (1) IL180694A (pt)
MX (1) MX2007001935A (pt)
RU (1) RU2359032C2 (pt)
WO (1) WO2006017960A1 (pt)
ZA (1) ZA200700269B (pt)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1294255C (zh) 2004-08-17 2007-01-10 广州科仁生物工程有限公司 一种具有转化黄曲霉毒素活性的解毒酶及编码该酶的基因
AU2008337569B2 (en) 2007-12-14 2013-12-19 Novozymes A/S Process for degrading zearalenone in a feed product employing laccase
US20090226570A1 (en) * 2008-03-05 2009-09-10 Novozymes A/S Detoxification of feed products
CN104130984B (zh) * 2014-07-30 2016-09-28 暨南大学 作用于杂色曲菌素的黄曲霉毒素氧化酶
CN104611305B (zh) * 2014-12-30 2017-11-17 暨南大学 胰蛋白酶抗性提高的黄曲霉毒素解毒酶
CN105112394A (zh) * 2015-09-11 2015-12-02 江南大学 一种能降解黄曲霉毒素b1的裂解酶的分离纯化方法
CN105400755B (zh) * 2015-12-09 2018-10-30 暨南大学 一种定点突变改造的人二肽基肽酶iii
CN105543250B (zh) * 2016-02-26 2018-12-21 广西大学 亮菌漆酶基因及其重组毕赤酵母工程菌和应用
CN107201377A (zh) * 2017-06-13 2017-09-26 山东仙普爱瑞科技股份有限公司 一种生产肝脏解毒酶的工程菌的构建及其应用
CN109370995B (zh) * 2018-12-11 2021-05-25 中国农业科学院北京畜牧兽医研究所 锰过氧化物酶CsMnP及其编码基因和应用
CN109557305B (zh) * 2019-01-09 2022-03-04 中粮集团有限公司 一种黄曲霉毒素b1降解酶及其应用和检测黄曲霉毒素b1的免疫层析试纸条
KR102114663B1 (ko) 2019-05-22 2020-05-25 한국식품연구원 아플라톡신 생성 균주의 생육억제 및 아플라톡신 생성 억제활성을 갖는 신규한 와이젤라 파라메센테로이데스 균주 및 이의 용도
CN115341010A (zh) * 2021-05-13 2022-11-15 华中农业大学 参与黄曲霉毒素b1毒性效应的靶点及其应用
CN114807074B (zh) * 2022-06-06 2023-10-13 江西省科学院生物资源研究所 一种黄曲霉毒素分解酶及其编码基因、重组载体、重组菌及应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5919685A (en) * 1997-08-08 1999-07-06 Incyte Pharmaceuticals, Inc. Human aflatoxin B1 aldehyde reductase
CN1113098C (zh) * 1998-06-30 2003-07-02 广东药学院 黄曲霉毒素b1的解毒酶提取工艺
CA2345203A1 (en) * 1998-10-07 2000-04-13 Maxygen Inc. Dna shuffling to produce nucleic acids for mycotoxin detoxification
CN1085057C (zh) * 1999-10-27 2002-05-22 广东省技术开发中心暨南大学 黄曲霉毒素b1解毒酶去除黄曲霉毒素b1的方法
CN1294255C (zh) 2004-08-17 2007-01-10 广州科仁生物工程有限公司 一种具有转化黄曲霉毒素活性的解毒酶及编码该酶的基因

Also Published As

Publication number Publication date
EP1780270A4 (en) 2007-10-24
JP2008509684A (ja) 2008-04-03
WO2006017960A1 (fr) 2006-02-23
CA2576069C (en) 2011-05-17
MX2007001935A (es) 2007-04-17
EP1780270A1 (en) 2007-05-02
CA2576069A1 (en) 2006-02-23
AU2005274578A1 (en) 2006-02-23
CN1294255C (zh) 2007-01-10
IL180694A0 (en) 2007-06-03
AU2005274578B2 (en) 2008-02-28
EP1780270B1 (en) 2010-11-24
HK1106272A1 (en) 2008-03-07
KR20070032796A (ko) 2007-03-22
RU2359032C2 (ru) 2009-06-20
US20080260711A1 (en) 2008-10-23
ZA200700269B (en) 2008-05-28
RU2007103735A (ru) 2008-09-27
ATE489458T1 (de) 2010-12-15
KR100850640B1 (ko) 2008-08-07
DE602005024973D1 (de) 2011-01-05
US7695751B2 (en) 2010-04-13
IL180694A (en) 2012-01-31
JP4495758B2 (ja) 2010-07-07
CN1712526A (zh) 2005-12-28

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Legal Events

Date Code Title Description
B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B04C Request for examination: application reinstated [chapter 4.3 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]
B07E Notification of approval relating to section 229 industrial property law [chapter 7.5 patent gazette]
B15K Others concerning applications: alteration of classification

Ipc: C12N 9/00 (2006.01), A61P 35/00 (2006.01)

B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

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