RU2007103735A - Детоксифизим с активностью преобразования афлатоксина и ген, кодирующий детоксифизим - Google Patents
Детоксифизим с активностью преобразования афлатоксина и ген, кодирующий детоксифизим Download PDFInfo
- Publication number
- RU2007103735A RU2007103735A RU2007103735/13A RU2007103735A RU2007103735A RU 2007103735 A RU2007103735 A RU 2007103735A RU 2007103735/13 A RU2007103735/13 A RU 2007103735/13A RU 2007103735 A RU2007103735 A RU 2007103735A RU 2007103735 A RU2007103735 A RU 2007103735A
- Authority
- RU
- Russia
- Prior art keywords
- aflatoxin
- conversion activity
- detoxifizime
- gene
- detoxifizyme
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/43—Enzymes; Proenzymes; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/02—Antidotes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Physics & Mathematics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Epidemiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Peptides Or Proteins (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Claims (8)
1. Детоксифизим с активностью преобразования афлатоксина, изоэлектрическая точка которого составляет 5,3-6,8, и его молекулярная масса составляет 73-77 кДа и его аминокислотная последовательность представлена в Списке последовательностей.
2. Ген, содержащий нуклеотидную последовательность, кодирующую детоксифизим с активностью преобразования афлатоксина по п.1.
3. Ген по п.2, который содержит нуклеотидную последовательность, представленную в Списке последовательностей.
4. Рекомбинантный экспрессионный вектор, содержащий ген по п.3.
5. Трансформант, полученный трансформацией клетки-хозяина с использованием рекомбинантного экспрессионного вектора по п.4.
6. Способ получения детоксифизима по п.1, способ содержащий:
культивирование трансформанта по п.5; и
выделение, очистку и восстановление экспрессированного детоксифизима с активностью преобразования афлатоксина.
7. Применение детоксифизима с активностью преобразования афлатоксина по п.1 для производства кормов и пищевых продуктов.
8. Применение детоксифизима с активностью преобразования афлатоксина по п.1 для получения лекарственного средства для предупреждения или лечения опухоли или злокачественной опухоли.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100511200A CN1294255C (zh) | 2004-08-17 | 2004-08-17 | 一种具有转化黄曲霉毒素活性的解毒酶及编码该酶的基因 |
| CN200410051120.0 | 2004-08-17 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2007103735A true RU2007103735A (ru) | 2008-09-27 |
| RU2359032C2 RU2359032C2 (ru) | 2009-06-20 |
Family
ID=35718321
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2007103735/13A RU2359032C2 (ru) | 2004-08-17 | 2005-01-13 | Детоксифизим с активностью преобразования афлатоксина и ген, кодирующий детоксифизим |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US7695751B2 (ru) |
| EP (1) | EP1780270B1 (ru) |
| JP (1) | JP4495758B2 (ru) |
| KR (1) | KR100850640B1 (ru) |
| CN (1) | CN1294255C (ru) |
| AT (1) | ATE489458T1 (ru) |
| AU (1) | AU2005274578B2 (ru) |
| BR (1) | BRPI0513998A (ru) |
| CA (1) | CA2576069C (ru) |
| DE (1) | DE602005024973D1 (ru) |
| HK (1) | HK1106272A1 (ru) |
| IL (1) | IL180694A (ru) |
| MX (1) | MX2007001935A (ru) |
| RU (1) | RU2359032C2 (ru) |
| WO (1) | WO2006017960A1 (ru) |
| ZA (1) | ZA200700269B (ru) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105543250A (zh) * | 2016-02-26 | 2016-05-04 | 广西大学 | 亮菌漆酶基因及其重组毕赤酵母工程菌和应用 |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1294255C (zh) | 2004-08-17 | 2007-01-10 | 广州科仁生物工程有限公司 | 一种具有转化黄曲霉毒素活性的解毒酶及编码该酶的基因 |
| CA2708795A1 (en) | 2007-12-14 | 2009-06-25 | Novozymes A/S | Process for degrading zearalenone in a feed product employing laccase |
| EP2252163A2 (en) * | 2008-03-05 | 2010-11-24 | Novozymes A/S | Detoxification of aflatoxin in feed products |
| CN104130984B (zh) * | 2014-07-30 | 2016-09-28 | 暨南大学 | 作用于杂色曲菌素的黄曲霉毒素氧化酶 |
| CN104611305B (zh) * | 2014-12-30 | 2017-11-17 | 暨南大学 | 胰蛋白酶抗性提高的黄曲霉毒素解毒酶 |
| CN105112394A (zh) * | 2015-09-11 | 2015-12-02 | 江南大学 | 一种能降解黄曲霉毒素b1的裂解酶的分离纯化方法 |
| CN105400755B (zh) * | 2015-12-09 | 2018-10-30 | 暨南大学 | 一种定点突变改造的人二肽基肽酶iii |
| CN107201377A (zh) * | 2017-06-13 | 2017-09-26 | 山东仙普爱瑞科技股份有限公司 | 一种生产肝脏解毒酶的工程菌的构建及其应用 |
| CN109370995B (zh) * | 2018-12-11 | 2021-05-25 | 中国农业科学院北京畜牧兽医研究所 | 锰过氧化物酶CsMnP及其编码基因和应用 |
| CN109557305B (zh) * | 2019-01-09 | 2022-03-04 | 中粮集团有限公司 | 一种黄曲霉毒素b1降解酶及其应用和检测黄曲霉毒素b1的免疫层析试纸条 |
| KR102114663B1 (ko) | 2019-05-22 | 2020-05-25 | 한국식품연구원 | 아플라톡신 생성 균주의 생육억제 및 아플라톡신 생성 억제활성을 갖는 신규한 와이젤라 파라메센테로이데스 균주 및 이의 용도 |
| CN115341010A (zh) * | 2021-05-13 | 2022-11-15 | 华中农业大学 | 参与黄曲霉毒素b1毒性效应的靶点及其应用 |
| CN114807074B (zh) * | 2022-06-06 | 2023-10-13 | 江西省科学院生物资源研究所 | 一种黄曲霉毒素分解酶及其编码基因、重组载体、重组菌及应用 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5919685A (en) * | 1997-08-08 | 1999-07-06 | Incyte Pharmaceuticals, Inc. | Human aflatoxin B1 aldehyde reductase |
| CN1113098C (zh) * | 1998-06-30 | 2003-07-02 | 广东药学院 | 黄曲霉毒素b1的解毒酶提取工艺 |
| US6500639B2 (en) * | 1998-10-07 | 2002-12-31 | Maxygen, Inc. | DNA shuffling to produce nucleic acids for mycotoxin detoxification |
| CN1085057C (zh) * | 1999-10-27 | 2002-05-22 | 广东省技术开发中心暨南大学 | 黄曲霉毒素b1解毒酶去除黄曲霉毒素b1的方法 |
| CN1294255C (zh) | 2004-08-17 | 2007-01-10 | 广州科仁生物工程有限公司 | 一种具有转化黄曲霉毒素活性的解毒酶及编码该酶的基因 |
-
2004
- 2004-08-17 CN CNB2004100511200A patent/CN1294255C/zh not_active Expired - Lifetime
-
2005
- 2005-01-13 US US11/629,450 patent/US7695751B2/en not_active Expired - Fee Related
- 2005-01-13 WO PCT/CN2005/000050 patent/WO2006017960A1/zh active Application Filing
- 2005-01-13 KR KR1020077001737A patent/KR100850640B1/ko active IP Right Grant
- 2005-01-13 AT AT05700424T patent/ATE489458T1/de active
- 2005-01-13 CA CA2576069A patent/CA2576069C/en not_active Expired - Fee Related
- 2005-01-13 BR BRPI0513998-8A patent/BRPI0513998A/pt not_active Application Discontinuation
- 2005-01-13 EP EP05700424A patent/EP1780270B1/en not_active Not-in-force
- 2005-01-13 AU AU2005274578A patent/AU2005274578B2/en not_active Ceased
- 2005-01-13 RU RU2007103735/13A patent/RU2359032C2/ru not_active IP Right Cessation
- 2005-01-13 MX MX2007001935A patent/MX2007001935A/es active IP Right Grant
- 2005-01-13 DE DE602005024973T patent/DE602005024973D1/de active Active
- 2005-01-13 JP JP2007526165A patent/JP4495758B2/ja active Active
-
2007
- 2007-01-05 ZA ZA200700269A patent/ZA200700269B/en unknown
- 2007-01-14 IL IL180694A patent/IL180694A/en not_active IP Right Cessation
- 2007-10-30 HK HK07111708.3A patent/HK1106272A1/xx not_active IP Right Cessation
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105543250A (zh) * | 2016-02-26 | 2016-05-04 | 广西大学 | 亮菌漆酶基因及其重组毕赤酵母工程菌和应用 |
| CN105543250B (zh) * | 2016-02-26 | 2018-12-21 | 广西大学 | 亮菌漆酶基因及其重组毕赤酵母工程菌和应用 |
Also Published As
| Publication number | Publication date |
|---|---|
| RU2359032C2 (ru) | 2009-06-20 |
| EP1780270B1 (en) | 2010-11-24 |
| HK1106272A1 (en) | 2008-03-07 |
| IL180694A (en) | 2012-01-31 |
| KR100850640B1 (ko) | 2008-08-07 |
| EP1780270A4 (en) | 2007-10-24 |
| DE602005024973D1 (de) | 2011-01-05 |
| CN1294255C (zh) | 2007-01-10 |
| BRPI0513998A (pt) | 2008-05-20 |
| JP4495758B2 (ja) | 2010-07-07 |
| WO2006017960A1 (fr) | 2006-02-23 |
| AU2005274578A1 (en) | 2006-02-23 |
| CN1712526A (zh) | 2005-12-28 |
| AU2005274578B2 (en) | 2008-02-28 |
| EP1780270A1 (en) | 2007-05-02 |
| CA2576069C (en) | 2011-05-17 |
| KR20070032796A (ko) | 2007-03-22 |
| ZA200700269B (en) | 2008-05-28 |
| CA2576069A1 (en) | 2006-02-23 |
| IL180694A0 (en) | 2007-06-03 |
| ATE489458T1 (de) | 2010-12-15 |
| US20080260711A1 (en) | 2008-10-23 |
| JP2008509684A (ja) | 2008-04-03 |
| US7695751B2 (en) | 2010-04-13 |
| MX2007001935A (es) | 2007-04-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| RU2007103735A (ru) | Детоксифизим с активностью преобразования афлатоксина и ген, кодирующий детоксифизим | |
| JPWO2018124247A1 (ja) | 変異型ニトリルヒドラターゼ、該変異型ニトリルヒドラターゼをコードする核酸、該核酸を含む発現ベクター及び形質転換体、該変異型ニトリルヒドラターゼの製造方法、並びにアミド化合物の製造方法 | |
| CN105385693B (zh) | 一种高效表达多拷贝人表皮生长因子的方法 | |
| CA2331392A1 (en) | Coniothyrium minitans xylanase gene cxy1 | |
| KR100961528B1 (ko) | 대장균에서 활성형 인간 상피세포 성장인자의 대량제조방법 | |
| JP3355049B2 (ja) | 組換え人ミオグロビンの製造方法 | |
| KR100358948B1 (ko) | 인체 과립성 백혈구의 콜로니 자극인자를 분비하는 대장균 | |
| RU2004133974A (ru) | Полипептид, обладающий расщепляющей активностью по отношению к полисахариду, содержащему сульфатированную фукозу, способ его получения, кодирующие его последовательность днк и экспрессирующий вектор, способ получения трансформированной клетки e. coli | |
| CN101560248A (zh) | 减毒肠毒素c2超抗原突变蛋白及其制备方法和应用 | |
| CN116478969A (zh) | 胶原酶突变体、促进重组胶原酶分泌表达的方法及其应用 | |
| JP6116884B2 (ja) | 改良Ｆｃ結合性タンパク質およびその製造方法 | |
| WO2002099092A3 (en) | Production of recombinant human lysosomal alpha-mannosidase | |
| CA3057252A1 (en) | Expression and large-scale production of peptides | |
| CN103214562B (zh) | 减毒肠毒素c2超抗原突变蛋白及其制备方法和应用 | |
| ATE501257T1 (de) | Dna-sequenz und rekombinante herstellung von gruppe-4 majorallergenen aus getreiden | |
| KR100261987B1 (ko) | argU 유전자를 이용한 대장균에서 인간 인터페론-α 2A의 제조방법 | |
| JP2002523046A5 (ru) | ||
| JP2990280B1 (ja) | アミノペプチダ―ゼ及びその前駆体の遺伝子、該遺伝子を含むベクタ―並びに形質転換体 | |
| KR100204504B1 (ko) | 효모를 이용한 사람의 인터루킨-6-의 제조 방법 | |
| Heruth | Characterization of the genetic determinants for R body synthesis and assembly in Caedibacter taeniospiralis 47 and 116. | |
| TH14552EX (th) | ลำดับทางพันธุกรรมสำหรับ แอนติเจน ir-95 ชนิด 90k ที่พบร่วมกับเนื้องอก | |
| JPWO2021127457A5 (ru) | ||
| Park et al. | Cloning and Characterization of the Penicillium sp. α-Galactosidase cDNA | |
| ATE448311T1 (de) | Hybridprotein und dafür kodierender vektor zur inhibition der angiogenese | |
| TW200508390A (en) | Novel manganic type of superoxide dismutase from diatom and use thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | The patent is invalid due to non-payment of fees |
Effective date: 20190114 |