BRPI0407294A - Protease da famìlia de peptidases s2a e/ou famìlia de peptidases s1e, sequência de ácido nucleico isolada, construção de ácido nucleico, vetor de expressão recombinante, célula hospedeira recombinante, método para produzir um polipeptìdeo, planta transgênica, ou parte de planta, animal transgênico não-humano ou produtos ou elementos dos mesmos, uso de pelo menos um polipeptìdeo, aditivo de ração animal, composição de ração animal, e, composição detergente - Google Patents

Protease da famìlia de peptidases s2a e/ou famìlia de peptidases s1e, sequência de ácido nucleico isolada, construção de ácido nucleico, vetor de expressão recombinante, célula hospedeira recombinante, método para produzir um polipeptìdeo, planta transgênica, ou parte de planta, animal transgênico não-humano ou produtos ou elementos dos mesmos, uso de pelo menos um polipeptìdeo, aditivo de ração animal, composição de ração animal, e, composição detergente

Info

Publication number
BRPI0407294A
BRPI0407294A BR0407294-4A BRPI0407294A BRPI0407294A BR PI0407294 A BRPI0407294 A BR PI0407294A BR PI0407294 A BRPI0407294 A BR PI0407294A BR PI0407294 A BRPI0407294 A BR PI0407294A
Authority
BR
Brazil
Prior art keywords
animal feed
proteases
peptidase family
nocardiopsis
polypeptide
Prior art date
Application number
BR0407294-4A
Other languages
English (en)
Inventor
Peter Rahbek Oestergaard
Leonardo De Maria
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Publication of BRPI0407294A publication Critical patent/BRPI0407294A/pt
Publication of BRPI0407294B1 publication Critical patent/BRPI0407294B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it

Abstract

"PROTEASE DA FAMìLIA DE PEPTIDASES S2A E/OU FAMìLIA DE PEPTIDASES S1E, SEQuêNCIA DE áCIDO NUCLEICO ISOLADA, CONSTRUçãO DE áCIDO NUCLEICO, VETOR DE EXPRESSãO RECOMBINANTE, CéLULA HOSPEDEIRA RECOMBINANTE, MéTODO PARA PRODUZIR UM POLIPEPTìDEO, PLANTA TRANSGêNICA, OU PARTE DE PLANTA, ANIMAL TRANSGêNICO NãO-HUMANO OU PRODUTOS OU ELEMENTOS DOS MESMOS, USO DE PELO MENOS UM POLIPEPTìDEO, ADITIVO DE RAçãO ANIMAL, COMPOSIçãO DE RAçãO ANIMAL, E, COMPOSIçãO DETERGENTE". A invenção refere-se a uma nova classe de serina proteases da família de peptidases S2A ou S1E que são estáveis na presença de cobre (Cu2+) e/ou que são inibidas pelo cobre apenas em grau reduzido. Características estruturais de relevância potencial para este efeito também são divulgadas. Esta classe de proteases inclui proteases derivadas de Brachysporiella gayana, Nocardiopsis dassonvillei subespécie dassonvillei, Nocardiopsis prasina, e Nocardiopsis alba, porém também exclui proteases conhecidas derivadas de Metarhizium anisopliae e Nocardiopsis sp. NRRL 18262. A invenção refere-se também a DNA que codifica referidas proteases, à expressão das mesmas em uma célula hospedeira, incluindo A invenção refere-se a uma nova classe de serina proteases da família de peptidases S2A ou S1E que são estáveis na presença de cobre (Cu2+) e/ou que são inibidas pelo cobre apenas em grau reduzido. Características estruturais de relevância potencial para este efeito também são divulgadas. Esta classe de proteases inclui proteases derivadas de Brachysporiella gayana, Nocardiopsis dassonvillei subespécie dassonvillei, Nocardiopsis prasina, e Nocardiopsis alba, porém também exclui proteases conhecidas derivadas de Metarhizilím anisopliae e Nocardiopsis sp. NRRL 18262. A invenção refere-se também a DNA que codifica referidas proteases, à expressão das mesmas em uma célula hospedeira, incluindo células de planta e animais, assim como o seu uso, por exemplo em ração animal e em detergentes. Em particular, a invenção se refere à ração animal e aos aditivos de ração animal, tais como pré-mistura, incorporando essas proteases junto com 1-500 ppm Cu (concentração na ração).
BRPI0407294-4A 2003-02-07 2004-02-09 Use of a protease of the peptidases family s2a and / or peptidasis fmilia s1e, method for producing polipeptide, animal feed additive, animal feed composition, and, detergent composition BRPI0407294B1 (pt)

Applications Claiming Priority (17)

Application Number Priority Date Filing Date Title
DKPA200300173 2003-02-07
DKPA200300173 2003-02-07
DKPA200300426 2003-03-20
DKPA200300426 2003-03-20
DKPA200300912 2003-06-19
DKPA200300915 2003-06-19
DKPA200300914 2003-06-19
DKPA200300915 2003-06-19
DKPA200300912 2003-06-19
DKPA200300913 2003-06-19
DKPA200300914 2003-06-19
DKPA200300913 2003-06-19
DKPA200301492 2003-10-10
DKPA200301493 2003-10-10
DKPA200301493 2003-10-10
DKPA200301492 2003-10-10
PCT/DK2004/000090 WO2004072221A2 (en) 2003-02-07 2004-02-09 Proteases

Publications (2)

Publication Number Publication Date
BRPI0407294A true BRPI0407294A (pt) 2006-02-07
BRPI0407294B1 BRPI0407294B1 (pt) 2017-06-13

Family

ID=32873147

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0407294-4A BRPI0407294B1 (pt) 2003-02-07 2004-02-09 Use of a protease of the peptidases family s2a and / or peptidasis fmilia s1e, method for producing polipeptide, animal feed additive, animal feed composition, and, detergent composition

Country Status (10)

Country Link
US (3) US7588926B2 (pt)
EP (1) EP1594963B1 (pt)
CN (1) CN1774504B (pt)
AT (1) ATE368105T1 (pt)
AU (1) AU2004211446B2 (pt)
BR (1) BRPI0407294B1 (pt)
DE (1) DE602004007766T2 (pt)
DK (1) DK1594963T3 (pt)
ES (1) ES2291883T3 (pt)
WO (1) WO2004072221A2 (pt)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6855548B2 (en) 2000-02-08 2005-02-15 F. Hoffman-La Roche Ag Use of acid-stable proteases in animal feed
US6960462B2 (en) * 2000-02-08 2005-11-01 Dsm Ip Assets B.V Use of acid-stable subtilisin proteases in animal feed
BRPI0407294B1 (pt) 2003-02-07 2017-06-13 Novozymes A/S Use of a protease of the peptidases family s2a and / or peptidasis fmilia s1e, method for producing polipeptide, animal feed additive, animal feed composition, and, detergent composition
ATE463563T1 (de) * 2003-06-19 2010-04-15 Novozymes As Verbesserte proteasen und verfahren zu ihrer herstellung
WO2004111223A1 (en) * 2003-06-19 2004-12-23 Novozymes A/S Proteases
EP1639107B1 (en) 2003-06-19 2013-08-14 Novozymes A/S Improved proteases and methods for producing them
WO2004111221A1 (en) * 2003-06-19 2004-12-23 Novozymes A/S Proteases
US7179630B2 (en) * 2003-06-19 2007-02-20 Novozymes A/S Thermostable proteases
WO2004111222A1 (en) * 2003-06-19 2004-12-23 Novozymes A/S Proteases
ES2358092T3 (es) * 2003-10-10 2011-05-05 Novozymes A/S Variantes de proteasa.
MXPA06014649A (es) * 2004-06-21 2007-03-12 Novozymes As Proteasas.
US20080044853A1 (en) * 2004-06-21 2008-02-21 Novozymes A/S Stably Maintained Multiple Copies of at Least Two Orf in the Same Orientation
ATE459720T1 (de) * 2004-08-18 2010-03-15 Bayer Cropscience Ag Pflanzen mit erhöhter plastidär aktivität der stärkephosphorylierenden r3-enzyme
US9668501B2 (en) 2006-07-13 2017-06-06 Dsm Ip Assets B.V. Use of bacterial amylases in feed for bovine animals
US9578891B2 (en) 2006-08-07 2017-02-28 Novozymes A/S Enzyme granules for animal feed
CN101505611B (zh) 2006-08-07 2013-03-27 诺维信公司 用于动物饲料的酶团粒
WO2008057293A2 (en) * 2006-10-27 2008-05-15 E. I. Du Pont De Nemours And Company Methods and compositions for prion decontamination
CN101558148B (zh) * 2006-12-22 2014-05-21 诺维信公司 用于产生酵母提取物的方法
US8691743B2 (en) 2008-05-14 2014-04-08 Novozymes A/S Liquid detergent compositions
ATE538660T1 (de) * 2008-06-03 2012-01-15 Novozymes As Verfahren zur herstellung eines weizenproteinhydrolysats
EP2299839A2 (en) * 2008-06-20 2011-03-30 Solae, Llc Protein hydrolysate compositions stable under acidic conditions
DK2398893T3 (en) * 2009-02-19 2015-03-23 Novozymes As PROCESS FOR BREWING FOR USING fungal or Bacterial
AU2010230165B2 (en) * 2009-04-02 2015-02-12 Novozymes A/S Process for making a milk-based protein hydrolysate
EP2744900B1 (en) 2011-08-19 2017-07-19 Novozymes A/S Polypeptides having protease activity
EP2751266B1 (en) 2011-09-22 2017-03-29 Novozymes A/S Polypeptides having protease activity and polynucleotides encoding same
US10174301B2 (en) 2011-12-28 2019-01-08 Novozymes A/S Methods for improving the nutritional value of the animal feed using a protease
US9801398B2 (en) 2012-01-26 2017-10-31 Novozymes A/S Use of polypeptides having protease activity in animal feed and detergents
MX364390B (es) 2012-06-20 2019-04-25 Novozymes As Uso de polipeptidos que tienen actividad proteasa en alimentos para animales y detergentes.
US9771570B2 (en) 2012-09-05 2017-09-26 Novozymes A/S Polypeptides having protease activity
WO2014055778A2 (en) 2012-10-03 2014-04-10 Agrivida, Inc. Multiprotein expression cassettes
FR2998450A1 (fr) * 2012-11-26 2014-05-30 Dominique Hooreman Utilisation d'un complexe enzymatique dans l'alimentation des animaux d'elevage
CN104869841A (zh) 2012-12-21 2015-08-26 诺维信公司 具有蛋白酶活性的多肽和编码它的多核苷酸
AU2014214032A1 (en) 2013-02-06 2015-07-09 Novozymes A/S Use of polypeptides having protease activity in animal feed
CN106455630B (zh) 2014-06-27 2021-08-24 帝斯曼知识产权资产管理有限公司 改进动物饲料的营养价值的方法
CN104293760A (zh) * 2014-10-14 2015-01-21 夏盛（武汉）生物科技有限公司 一种多酶复合型抑泡剂及其在酒精发酵工业中的应用
WO2016071302A1 (en) 2014-11-04 2016-05-12 Novozymes A/S Polypeptides having serine protease activity and polynucleotides encoding same and their application in animal feed
TW201940778A (zh) 2018-01-16 2019-10-16 日商花王股份有限公司 角質汙垢清潔劑、及角質汙垢分解能力之評估方法
WO2020074517A1 (en) * 2018-10-10 2020-04-16 Novozymes A/S Chymotrypsin inhibitor variants and the use thereof
CN110283880A (zh) * 2019-08-08 2019-09-27 天津科技大学 一种测定蛋白酶活力的新方法

Family Cites Families (57)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3668448A (en) * 1967-05-29 1972-06-06 Enakichi Hayasaka Homopole type ac generator
GB1234445A (pt) 1967-10-03 1971-06-03
DE2004328C3 (de) 1970-01-30 1979-10-18 Nippon Kokan K.K., Tokio Bodenkonstruktion für eine Gas-Flüssigkeits-Kontaktierkolonne
US3823072A (en) 1970-02-19 1974-07-09 M Hooreman Analysis of proteolytic enzymes
US3683069A (en) 1970-02-19 1972-08-08 Michel Hooreman Enzymatic products which optimize the viscosity of mucuses and their applications
US3663069A (en) * 1970-10-07 1972-05-16 Kelsey Hayes Co Skid control system
US3868448A (en) 1973-12-07 1975-02-25 Grace W R & Co Method for bloat control
US3966971A (en) 1975-01-02 1976-06-29 Grain Processing Corporation Separation of protein from vegetable sources
US4073884A (en) 1976-09-27 1978-02-14 W. R. Grace & Co. Promotion of feed efficiency
DE3149457A1 (de) 1981-12-14 1983-06-23 Henkel KGaA, 4000 Düsseldorf Verfahren zur herstellung saurer protease und diese bildende mutanten von pilzen der gattung aspergillus
IE81141B1 (en) 1983-06-24 2000-04-05 Genencor Int Procaryotic carbonyl hydrolases
CA1295566C (en) 1987-07-21 1992-02-11 Robert T. Garvin Characterization and structure of genes for protease a and protease b from streptomyces griseus
FR2600340A1 (fr) 1986-06-20 1987-12-24 Hooremam Michel Procede de production d'un nouveau complexe proteolytique stimulant l'activite du pancreas et ses applications en zootechnie
DK564086A (da) 1986-11-25 1988-06-17 Novo Industri As Enzymatisk detergent-additiv
JPH02255081A (ja) 1989-03-30 1990-10-15 Snow Brand Milk Prod Co Ltd プロテアーゼ生産菌、該菌体から産生したプロテアーゼ、及びその精製方法
DK316989D0 (da) 1989-06-26 1989-06-26 Novo Nordisk As Enzymer
WO1991010723A1 (en) 1990-01-11 1991-07-25 Novo Nordisk A/S Bacteriolytic enzyme native to a nocardiopsis strain, its production and use.
CA2063892A1 (en) 1991-03-29 1992-09-30 Susan A. Anderson Alpha-lytic protease cleaning compositions
DE69212390T2 (de) 1991-05-01 1997-01-09 Unilever Nv Stabilisierte Enzyme enthaltende Waschmittelzusammensetzungen
DK0583339T3 (da) 1991-05-01 1999-04-19 Novo Nordisk As Stabiliserede enzymer og detergentsammensætninger
US5646028A (en) 1991-06-18 1997-07-08 The Clorox Company Alkaline serine protease streptomyces griseus var. alkaliphus having enhanced stability against urea or guanidine
US5811382A (en) 1991-12-20 1998-09-22 Novo Nordisk A/S Detergent compositions
ATE214731T1 (de) 1992-05-19 2002-04-15 Shionogi & Co Neuartige protease
DK81193D0 (da) 1993-07-06 1993-07-06 Novo Nordisk As Enzym
CA2158752A1 (en) 1994-02-14 1995-08-17 Patrick S. Lee Animal food palatability improving composition and process
AU692859B2 (en) 1994-04-22 1998-06-18 Novozymes A/S A method for improving the solubility of vegetable proteins
GB9416841D0 (en) 1994-08-19 1994-10-12 Finnfeeds Int Ltd An enzyme feed additive and animal feed including it
US5877403A (en) 1994-12-30 1999-03-02 Seminis Vegetable Seeds, Inc. Papaya ringspot virus protease gene
DK1396A (pt) 1996-01-09 1996-01-09 Novo Nordisk As
US5705379A (en) * 1996-10-23 1998-01-06 Cornell Research Foundation, Inc. Nucleotide sequences encoding a thermostable alkaline protease
US6068630A (en) * 1997-01-02 2000-05-30 St. Francis Medical Technologies, Inc. Spine distraction implant
FI972521A (fi) 1997-06-13 1998-12-14 Cultor Oy Menetelmä ravinnollisen tuotteen valmistamiseksi ja näin valmistettu tuote
NZ330940A (en) 1997-07-24 2000-02-28 F Production of consensus phytases from fungal origin using computer programmes
US6835550B1 (en) 1998-04-15 2004-12-28 Genencor International, Inc. Mutant proteins having lower allergenic response in humans and methods for constructing, identifying and producing such proteins
US6855548B2 (en) 2000-02-08 2005-02-15 F. Hoffman-La Roche Ag Use of acid-stable proteases in animal feed
US6960462B2 (en) * 2000-02-08 2005-11-01 Dsm Ip Assets B.V Use of acid-stable subtilisin proteases in animal feed
MX261332B (es) * 2000-02-08 2008-10-14 Hoffmann La Roche Uso de proteasas estables en acido para alimento de animales.
US7630836B2 (en) * 2001-05-30 2009-12-08 The Kitasato Institute Polynucleotides
JP4137526B2 (ja) 2002-01-23 2008-08-20 Ｔｏｔｏ株式会社 ケラチナーゼおよびその製造法
JP4114046B2 (ja) 2002-07-12 2008-07-09 川村通商株式会社 酵素脱毛処理剤および酵素脱毛法
JP4344950B2 (ja) 2003-02-06 2009-10-14 大和化成株式会社 獣毛繊維の防縮加工法
BRPI0407294B1 (pt) * 2003-02-07 2017-06-13 Novozymes A/S Use of a protease of the peptidases family s2a and / or peptidasis fmilia s1e, method for producing polipeptide, animal feed additive, animal feed composition, and, detergent composition
WO2004072279A2 (en) 2003-02-07 2004-08-26 Novozymes A/S Proteases
EP1639107B1 (en) * 2003-06-19 2013-08-14 Novozymes A/S Improved proteases and methods for producing them
ATE463563T1 (de) * 2003-06-19 2010-04-15 Novozymes As Verbesserte proteasen und verfahren zu ihrer herstellung
WO2004111221A1 (en) 2003-06-19 2004-12-23 Novozymes A/S Proteases
WO2004111222A1 (en) 2003-06-19 2004-12-23 Novozymes A/S Proteases
WO2004111223A1 (en) 2003-06-19 2004-12-23 Novozymes A/S Proteases
US7179630B2 (en) * 2003-06-19 2007-02-20 Novozymes A/S Thermostable proteases
US7208310B2 (en) 2003-06-19 2007-04-24 Novozymes A/S Proteases
US7935501B2 (en) * 2003-09-11 2011-05-03 Novozymes Pharma Biotec A/S Recombinant production of antimicrobial peptides
ES2358092T3 (es) * 2003-10-10 2011-05-05 Novozymes A/S Variantes de proteasa.
MXPA06014649A (es) 2004-06-21 2007-03-12 Novozymes As Proteasas.
CN101010424A (zh) * 2004-06-21 2007-08-01 诺维信公司 生产异源蛋白酶的改进方法
US7618801B2 (en) * 2007-10-30 2009-11-17 Danison US Inc. Streptomyces protease
US20100093633A1 (en) * 2008-10-10 2010-04-15 Novozymes A/S Polypeptides having antimicrobial activity
AU2010230165B2 (en) * 2009-04-02 2015-02-12 Novozymes A/S Process for making a milk-based protein hydrolysate

Also Published As

Publication number Publication date
US7588926B2 (en) 2009-09-15
US20090238922A1 (en) 2009-09-24
US7906310B2 (en) 2011-03-15
CN1774504A (zh) 2006-05-17
US20060147499A1 (en) 2006-07-06
BRPI0407294B1 (pt) 2017-06-13
AU2004211446A1 (en) 2004-08-26
US20110124084A1 (en) 2011-05-26
EP1594963A2 (en) 2005-11-16
ES2291883T3 (es) 2008-03-01
WO2004072221A2 (en) 2004-08-26
ATE368105T1 (de) 2007-08-15
DK1594963T3 (da) 2007-11-26
DE602004007766D1 (de) 2007-09-06
EP1594963B1 (en) 2007-07-25
CN1774504B (zh) 2013-04-17
AU2004211446B2 (en) 2009-05-28
DE602004007766T2 (de) 2008-06-05
WO2004072221A3 (en) 2004-09-30

Similar Documents

Publication Publication Date Title
BRPI0407294A (pt) Protease da famìlia de peptidases s2a e/ou famìlia de peptidases s1e, sequência de ácido nucleico isolada, construção de ácido nucleico, vetor de expressão recombinante, célula hospedeira recombinante, método para produzir um polipeptìdeo, planta transgênica, ou parte de planta, animal transgênico não-humano ou produtos ou elementos dos mesmos, uso de pelo menos um polipeptìdeo, aditivo de ração animal, composição de ração animal, e, composição detergente
Menezes et al. Sex-based individual variation of snake venom proteome among eighteen Bothrops jararaca siblings
BRPI0410797A (pt) polipeptìdeo isolado, seqüência de ácido nucleico isolado, construção de ácido nucleico, vetor de expressão recombinante, célula hospedeira recombinante, método para a produção de um polipeptìdeo, planta transgênica ou, parte da planta, animal transgênico, não-humano, ou produtos, ou elementos deste, uso de pelo menos um polipeptìdeo, aditivo para ração para animal, composição, e, microorganismo
Bernardoni et al. Functional variability of snake venom metalloproteinases: adaptive advantages in targeting different prey and implications for human envenomation
BRPI0512341A (pt) polipeptìdeo isolado tendo atividade de protease, seqüência de ácido nucleico isolada, construto de ácido nucleico, vetor de expressão, célula hospedeira recombinante, planta ou parte vegetal transgênica, animal não humano transgênico, uso de pelo menos uma protease, métodos para produzir um polipeptìdeo, para melhorar o valor nutricional de uma ração animal e para tratar proteìnas, ração animal, e, aditivo de ração animal
WO2004072279A3 (en) Proteases
ME00326B (me) Anti-il-12-antitjela, kompozicije, metode i njihove upotrebe
DE69226182T2 (de) Stabilisierte enzyme und waschmittelzusammensetzungen
Moura-da-Silva et al. Evidence for heterogeneous forms of the snake venom metalloproteinase jararhagin: a factor contributing to snake venom variability
ATE530643T1 (de) Säureaktive fungale protease
ATE491788T1 (de) Proteasevarianten
BRPI0416797A (pt) serina proteases, ácidos nucléicos codificando enzimas de serina e vetores e células hospedeiras incorporando as mesmas
ATE519842T1 (de) Trichoderma reesei-glucoamylase und homologe davon
Chiappini et al. Streptococcus pyogenes SpyCEP influences host-pathogen interactions during infection in a murine air pouch model
DE69034013T2 (de) Serpin-resistenter T-PA; Mutanten; Gene
Vaiyapuri et al. Purification and functional characterisation of rhinocerase, a novel serine protease from the venom of Bitis gabonica rhinoceros
BR0009516A (pt) Fragmento de fosfatase tendo atividade da fitasemelhorada, alimentação de animal melhorada,gene recombinante, vetor, célula hospedeira,método para aumentar a atividade da fitase defosfatase, fosfatase, e molécula de ácido nucleicoisolada
DE602004027376D1 (de) Proteasen
Mechri et al. Cloning and heterologous expression of subtilisin SAPN, a serine alkaline protease from Melghiribacillus thermohalophilus Nari2AT in Escherichia coli and Pichia pastoris
Chagas et al. An insight into the sialome of Simulium guianense (DIPTERA: SIMulIIDAE), the main vector of River Blindness Disease in Brazil
ATE365173T1 (de) Previns als spezifische inhibitoren und therapeutika für botulinumtoxin b und tetanusneurotoxine
Zelanis et al. Variability in expression of Bothrops insularis snake venom proteases: an ontogenetic approach
ATE452184T1 (de) Mikrobielle trypsinvarianten mit chymotrypsinaktivität und diese codierende nukleinsäuren
Larsen et al. Characterization of a recombinantly expressed proteinase K‐like enzyme from a psychrotrophic Serratia sp.
KR102588719B1 (ko) 향상된 단백질 발현

Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B11E Dismissal acc. art. 34 of ipl - requirements for examination incomplete
B11M Decision cancelled [chapter 11.13 patent gazette]

Free format text: O PRESENTE PEDIDO TEVE SEU ARQUIVAMENTO NOTIFICADO NA RPI NO 2263 DE 20/05/2014 (DESPACHO 11.5), DE ACORDO COM O ART. 34 DA LPI. NO ENTANTO, O REQUERENTE ATENDEU AOS PRESSUPOSTOS DO ART. 34 DA LPI, TEMPESTIVAMENTE, ATRAVES DA PETICAO NO 020130096150 DE 30/12/2013 (LISTAGEM DE SEQUENCIAS). DESTE MODO, ANULO O REFERIDO ARQUIVAMENTO.

B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]
B21F Lapse acc. art. 78, item iv - on non-payment of the annual fees in time

Free format text: REFERENTE A 15A ANUIDADE.

B24J Lapse because of non-payment of annual fees (definitively: art 78 iv lpi, resolution 113/2013 art. 12)

Free format text: EM VIRTUDE DA EXTINCAO PUBLICADA NA RPI 2500 DE 04-12-2018 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDA A EXTINCAO DA PATENTE E SEUS CERTIFICADOS, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.