BR9811531A - Preparação de proteìnas mutadas humanas em células humanas por meio de recombinação homóloga - Google Patents

Preparação de proteìnas mutadas humanas em células humanas por meio de recombinação homóloga

Info

Publication number
BR9811531A
BR9811531A BR9811531-6A BR9811531A BR9811531A BR 9811531 A BR9811531 A BR 9811531A BR 9811531 A BR9811531 A BR 9811531A BR 9811531 A BR9811531 A BR 9811531A
Authority
BR
Brazil
Prior art keywords
human
preparation
homologous recombination
human cells
cells
Prior art date
Application number
BR9811531-6A
Other languages
English (en)
Inventor
Anne Stern
Konrad Honold
Original Assignee
Roche Diagnostics Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8227102&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BR9811531(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Roche Diagnostics Gmbh filed Critical Roche Diagnostics Gmbh
Publication of BR9811531A publication Critical patent/BR9811531A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Abstract

Patente de Invenção: <B>''PREPARAçãO DE PROTEìNAS MUTADAS HUMANAS EM CéLULAS HUMANAS POR MEIO DE RECOMBINAçãO HOMóLOGA''<D>. A invenção refere-se a um processo para a preparação de muteínas de polipeptídios eucariónticos em células eucariónticas por meio de recombinação homóloga. Além disso, a invenção refere-se a um processo para a preparação de células humanas, que são adequadas para a preparação de proteínas mutadas humanas. Finalmente, a invenção refere-se às células humanas preparadas pelo processo e proteínas humanas mutadas obteníveis a partir das mesmas, bem como a preparados farmacêuticos, que contêm essas muteínas.
BR9811531-6A 1997-07-23 1998-07-22 Preparação de proteìnas mutadas humanas em células humanas por meio de recombinação homóloga BR9811531A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP97112639 1997-07-23
PCT/EP1998/004583 WO1999005264A2 (de) 1997-07-23 1998-07-22 Herstellung humaner mutierter proteine in humanen zellen mittels homologer rekombination

Publications (1)

Publication Number Publication Date
BR9811531A true BR9811531A (pt) 2000-08-22

Family

ID=8227102

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9811531-6A BR9811531A (pt) 1997-07-23 1998-07-22 Preparação de proteìnas mutadas humanas em células humanas por meio de recombinação homóloga

Country Status (17)

Country Link
US (2) US6444441B1 (pt)
EP (1) EP1000167B2 (pt)
JP (1) JP2001511341A (pt)
KR (1) KR100512018B1 (pt)
CN (2) CN1265152A (pt)
AR (2) AR013251A1 (pt)
AT (1) ATE313643T1 (pt)
AU (1) AU729489B2 (pt)
BR (1) BR9811531A (pt)
CA (1) CA2297660C (pt)
DE (1) DE59813296D1 (pt)
DK (1) DK1000167T4 (pt)
ES (1) ES2255177T5 (pt)
PT (1) PT1000167E (pt)
TR (1) TR200000172T2 (pt)
WO (1) WO1999005264A2 (pt)
ZA (1) ZA986517B (pt)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108700568A (zh) * 2016-01-06 2018-10-23 隆扎有限公司 用于改善的生产的蛋白质降解抑制剂

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5234686A (en) * 1985-04-03 1993-08-10 Beecham Group P.L.C. Human tissue plasminogen activator consisting essentially of t-PA residues to 160 to 527, pharmaceutical compositions and methods of treatment
US5094953A (en) * 1988-03-21 1992-03-10 Genentech, Inc. Human tissue plasminogen activator variants
DE3903581A1 (de) * 1989-02-07 1990-08-16 Boehringer Mannheim Gmbh Gewebs-plasminogenaktivator-derivat
DE3907679A1 (de) * 1989-03-09 1990-09-13 Max Planck Gesellschaft Verfahren zur gezielten veraenderung eines gens
JPH0576361A (ja) * 1990-05-10 1993-03-30 Takeda Chem Ind Ltd 組織プラスミノーゲンアクチベータームテイン
DE69133557D1 (de) * 1990-08-29 2007-03-15 Pharming Intellectual Pty Bv Homologe rekombination in säugetier-zellen
EP0539573A4 (en) * 1991-05-15 1993-12-29 Cell Genesys, Inc. Genomic modifications with homologous dna targeting
US5641670A (en) 1991-11-05 1997-06-24 Transkaryotic Therapies, Inc. Protein production and protein delivery
US6270989B1 (en) * 1991-11-05 2001-08-07 Transkaryotic Therapies, Inc. Protein production and delivery
AU4115693A (en) * 1992-04-24 1993-11-29 Sri International In vivo homologous sequence targeting in eukaryotic cells
EP0656747A4 (en) * 1992-08-21 1997-05-07 Univ California COMPOSITION AND METHOD FOR CHANGING DNA SEQUENCES BY HOMOLOGOUS RECOMBINATION.
TW402639B (en) * 1992-12-03 2000-08-21 Transkaryotic Therapies Inc Protein production and protein delivery
CN1256986C (zh) * 1993-12-02 2006-05-24 转移染色体治疗公司 蛋白质的生产和蛋白质的输送
AU6328696A (en) * 1995-06-07 1996-12-30 Yale University Treatment of hemoglobinopathies
US6087129A (en) * 1996-01-19 2000-07-11 Betagene, Inc. Recombinant expression of proteins from secretory cell lines

Also Published As

Publication number Publication date
CN101348789A (zh) 2009-01-21
PT1000167E (pt) 2006-05-31
EP1000167B2 (de) 2011-04-20
DK1000167T3 (da) 2006-04-24
EP1000167B1 (de) 2005-12-21
US6444441B1 (en) 2002-09-03
ATE313643T1 (de) 2006-01-15
JP2001511341A (ja) 2001-08-14
US20020177195A1 (en) 2002-11-28
ZA986517B (en) 2000-01-24
WO1999005264A3 (de) 1999-04-22
KR100512018B1 (ko) 2005-09-02
AR013251A1 (es) 2000-12-13
CN101348789B (zh) 2016-08-17
DE59813296D1 (de) 2006-01-26
CA2297660C (en) 2010-04-27
DK1000167T4 (da) 2011-08-08
AR068104A2 (es) 2009-11-04
KR20010022127A (ko) 2001-03-15
AU729489B2 (en) 2001-02-01
TR200000172T2 (tr) 2000-12-21
CN1265152A (zh) 2000-08-30
AU9067398A (en) 1999-02-16
WO1999005264A2 (de) 1999-02-04
EP1000167A2 (de) 2000-05-17
ES2255177T3 (es) 2006-06-16
US20020068318A1 (en) 2002-06-06
CA2297660A1 (en) 1999-02-04
ES2255177T5 (es) 2011-07-12

Similar Documents

Publication Publication Date Title
BR9814404A (pt) &#34; clonagem de expressão em fungos filamentosos &#34;
BR0011499A (pt) Moléculas de ácido nucléico oriundas de plantas e células de,planta transgênicas de trigo e o seu uso para a produção de amido modificado
BR0308919A (pt) Células-tronco programáveis, desdiferenciadas de origem monocìtica, e sua produção e uso
AU2001278797A1 (en) Expression vector using for animal cell
DK0559884T3 (da) Rekombinante virusvektorer til ekspression i muskelceller
DK58789A (da) Proteiner med faktor viii-aktivitet, fremgangsmaade til deres fremstilling ved anvendelse af genetisk konstruerede celler og farmaceutiske kompositioner indeholdende proteinerne
ATE404583T1 (de) Funktionelle antagonisten von hedgehog-aktivität
FI963070A0 (fi) Klooripyrimidiinivälituotteita
ES2153444T3 (es) Anticuerpos contra interleucina-4 mamifera, y peptidos utiles como antigenos en su produccion.
DK1200583T3 (da) Fremstilling af beta-foldeblad-proteiner med specifikke bindingsegenskaber
BR9812547A (pt) Proteìnas de fusão compreendendo proteìnas hiv-1 tat e/ou nef
ES2147720T3 (es) Factor de celulas madre.
DE68918867D1 (de) Mutanten des hiv-1 Hüllproteins mit fehlenden hypervariabelen domänen.
IL175021A0 (en) Chromatin insulator, its preparation and use in protein expression
ATE437951T1 (de) Intrazelluläre herstellung von verkürztem hepatitis c-polypeptid e2
BR9712556A (pt) Plasmìdeo, composição farmacêutica, e, processo de imunizar um indivìduo contra um antìgeno
DK0851926T3 (da) Varianter af humant rekombinant gamminterferon (rhu-IFN-gamma) med forøget termisk stabilitet
DK124787A (da) Peptider indeholdende op til 8-aminosyregrupperinger og fysiologisk uskadelige salte deraf, deres fremstilling og anvendelse som laegemidler samt mellemprodukter ved fremstillingen
ATE101200T1 (de) Impfstoffe gegen menschliche respiratorische viren.
NO984491L (no) SCF-analogpreparater og fremgangsmÕte for fremstilling av disse
BR9811531A (pt) Preparação de proteìnas mutadas humanas em células humanas por meio de recombinação homóloga
AUPM772494A0 (en) Improvements in production of proteins in host cells
DE59712771D1 (de) Nebenhoden-spezifisches Rezeptorprotein und dessen Verwendung
DK0939769T3 (da) Vertebrat-Smoothened-proteiner
ES8605296A1 (es) Procedimiento de producir factor viii-r humano

Legal Events

Date Code Title Description
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: COM BASE NO ART. 8O COMBINADO COM ART. 13 DA LPI 9279/96 E DE ACORDO COM O ART. 37 DA LPI, SUGERE-SE O INDEFERIMENTO DO PRESENTE PEDIDO DE PATENTE.

B12B Appeal against refusal [chapter 12.2 patent gazette]