BR9712012A - Método para gerar birnavìrus a partir de trancritos de rna sintético. - Google Patents

Método para gerar birnavìrus a partir de trancritos de rna sintético.

Info

Publication number
BR9712012A
BR9712012A BR9712012-0A BR9712012A BR9712012A BR 9712012 A BR9712012 A BR 9712012A BR 9712012 A BR9712012 A BR 9712012A BR 9712012 A BR9712012 A BR 9712012A
Authority
BR
Brazil
Prior art keywords
rna
synthetic
segments
transcripts
virus
Prior art date
Application number
BR9712012-0A
Other languages
English (en)
Other versions
BR9712012B1 (pt
Inventor
Vikram N Vakharia
Egbert Mundt
Original Assignee
Univ Maryland Biotech Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Maryland Biotech Inst filed Critical Univ Maryland Biotech Inst
Publication of BR9712012A publication Critical patent/BR9712012A/pt
Publication of BR9712012B1 publication Critical patent/BR9712012B1/pt

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2720/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
    • C12N2720/00011—Details
    • C12N2720/10011—Birnaviridae
    • C12N2720/10051—Methods of production or purification of viral material
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S424/00—Drug, bio-affecting and body treating compositions
    • Y10S424/816—Viral vaccine for avian species, e.g. poultry or other birds
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S424/00—Drug, bio-affecting and body treating compositions
    • Y10S424/826—Bacterial vaccine for avian species, e.g. poultry or other birds

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Microbiology (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Oncology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Communicable Diseases (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Epidemiology (AREA)
  • Molecular Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

<B>"MéTODO PARA GERAR BIRNAVìRUS A PARTIR DE TRANSCRITOS DE RNA SINTéTICO"<D>. A presente invenção trata de um sistema que foi desenvolvido para a geração de Birnavírus ativo, tal como o vírus da doença bursal infecciosa (IBDV), um vírus segmentado de RNA duplamente espiralado (ds) da família dos Birnaviridae, usando transcritos sintéticos derivados de DNA clonado. Os clones de cDNA independentes de inteira extensão, foram construídos contendo regiões de inteira codificação e descodificação dos segmentos A e B do RNA, do IBDV, respectivamente. Os RNAs sintéticos de ambos os segmentos foram produzidos por meio de transcrição in vitro de plasmídios linearizados com polimerase de RNA T7. A transfecção das células de Vero combinado com os transcritos mais sensíveis de ambos os segmentos gerou o vírus infeccioso logo nas 36 horas após a transfecção. O desenvolvimento de um sistema genético inverso para vírus de RNA duplamente espiralados, irá facilitar acentuadamente as pesquisas de regulação da expressão do gene viral, da patogênese e o projeto de uma nova geração de vacinas ativas e inativadas.
BRPI9712012-0A 1996-09-05 1997-07-31 método para gerar ibdv quimérico a partir de transcritos de rna sintético de diferentes cepas de birnavìrus. BR9712012B1 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/708,541 US5871744A (en) 1996-09-05 1996-09-05 Method for generating birnavirus from synthetic RNA transcripts
PCT/US1997/012955 WO1998009646A1 (en) 1996-09-05 1997-07-31 A method for generating birnavirus from synthetic rna transcripts

Publications (2)

Publication Number Publication Date
BR9712012A true BR9712012A (pt) 2000-01-18
BR9712012B1 BR9712012B1 (pt) 2009-08-11

Family

ID=24846203

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI9712012-0A BR9712012B1 (pt) 1996-09-05 1997-07-31 método para gerar ibdv quimérico a partir de transcritos de rna sintético de diferentes cepas de birnavìrus.

Country Status (18)

Country Link
US (1) US5871744A (pt)
EP (2) EP0959901A4 (pt)
JP (1) JP4670025B2 (pt)
KR (1) KR100475427B1 (pt)
CN (1) CN1168500C (pt)
AT (1) ATE460178T1 (pt)
AU (1) AU741055B2 (pt)
BR (1) BR9712012B1 (pt)
CA (1) CA2264488C (pt)
CZ (1) CZ299417B6 (pt)
DE (1) DE69739805D1 (pt)
ES (1) ES2342325T3 (pt)
HU (1) HUP9904365A3 (pt)
IL (2) IL128804A0 (pt)
NO (1) NO991074L (pt)
NZ (1) NZ334667A (pt)
PL (1) PL190220B1 (pt)
WO (1) WO1998009646A1 (pt)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6596280B1 (en) * 1997-07-31 2003-07-22 University Of Maryland Biotechnology Institute Method for generating birnavirus from synthetic RNA transcripts
WO1999050419A2 (en) * 1998-03-31 1999-10-07 University Of Maryland Biotechnology Institute A method for generating nonpathogenic, infectious pancreatic necrosis virus (ipnv) from synthetic rna transcripts
WO2000012677A2 (en) * 1998-09-01 2000-03-09 The University Of Hong Kong Generation of recombinant infectious bursal disease viruses by reverse genetics technology and the use of the recombinant viruses as attenuated vaccines
HUP9802974A1 (hu) * 1998-12-19 2000-09-28 Mezőgazdasági Biotechnológiai Kutatóközpont Fertőző bursitis elleni DNS-vakcina
US6492148B1 (en) * 1999-03-05 2002-12-10 Akzo Nobel Nv Genetically engineered cell culture adapted infectious bursal disease virus (IBDV) mutants
US6468984B1 (en) 1999-06-08 2002-10-22 Innovo Biotechnologies Ltd. DNA vaccine for protecting an avian against infectious bursal disease virus
EP1069187A1 (en) * 1999-07-14 2001-01-17 Stichting Dienst Landbouwkundig Onderzoek Mosaic Infectious Bursal Disease Virus vaccines
US7431930B2 (en) * 2002-03-01 2008-10-07 Intervet International B.V. Infectious bursal disease virus (IBDV) mutant expressing virus neutralising epitopes specific for classic-and variant IBDV strains
ES2217967B1 (es) * 2003-03-31 2006-01-01 Consejo Sup. Investig. Cientificas Procedimiento de produccion de particulas virales vacias (vlps) del virus inductor de la bursitis infecciosa (ibdv), composiciones necesarias para su puesta a punto y su uso en la elaboracion de vacunas frente al ibdv.
ES2307346B1 (es) * 2004-01-21 2009-11-13 Consejo Sup. Investig. Cientificas Capsidas vacias (vlps(-vp4)) del virus causante de la enfermedad de la bursitis infecciosa (ibdv), su procedimiento de obtencion y aplicaciones.
ES2307345B1 (es) * 2004-01-21 2009-11-13 Consejo Sup. Investig. Cientificas Capsidas vacias quimericas del virus causante de la enfermedad de la bursitis infecciosa (ibdv), su procedimiento de obtencion y aplicaciones.
US7244432B2 (en) * 2004-12-08 2007-07-17 University Of Maryland Biotechnology Institute Infectious bursal disease virus (IBDV) variant from Georgia
ES2310062B1 (es) * 2005-07-15 2009-11-13 Bionostra, S.L. Particulas pseudovirales vacias quimericas derivadas del virus causante de la enfermedad de la bursitis infecciosa (ibdv), procedimiento de obtencion y aplicaciones.
KR100757541B1 (ko) * 2005-11-08 2007-09-10 엘지전자 주식회사 플라즈마 디스플레이 장치 및 그의 화상 처리방법
WO2008068661A1 (en) * 2006-12-01 2008-06-12 Hepc Biotechnológiai Kutató És Fejleszto Kft. Compositions and methods for the treatment of viral hepatitis
CN101016547B (zh) * 2007-01-25 2010-07-28 浙江大学 海洋双rna病毒mabv重组蛋白的制备方法与应用
EP2407534A1 (en) 2010-07-14 2012-01-18 Neo Virnatech, S.L. Methods and reagents for obtaining transcriptionally active virus-like particles and recombinant virions
CN109321583B (zh) * 2018-09-29 2023-08-15 福建省农业科学院畜牧兽医研究所 一种构建番鸭呼肠孤病毒反向遗传系统的方法
AU2020329166A1 (en) 2019-08-09 2022-03-03 Nutcracker Therapeutics, Inc. Microfluidic apparatus and methods of use thereof

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4530831A (en) * 1982-11-02 1985-07-23 Akzo N.V. Infectious Bursal Disease vaccine
CA1334941C (en) * 1985-05-30 1995-03-28 Ahmed Azad Azad Cloning and expression of a host-protective immunogens of ibdv
AU602875B2 (en) * 1985-12-18 1990-11-01 British Technology Group Limited Newcastle disease virus gene clones
US5192539A (en) * 1988-07-21 1993-03-09 Akzo N.V. Infectious bursal disease virus production in continuous cell lines
US5518724A (en) * 1988-08-02 1996-05-21 University Of Maryland Infectious bursal disease virus
NZ235474A (en) * 1989-10-18 1992-12-23 Univ Maryland Virus capable of inducing infectious bursal disease; assay and vaccine
JPH05501064A (ja) * 1990-05-04 1993-03-04 ユニバーシティ　オブ　メリーランド　アット　カレッジ　パーク Ｉｂｄｖタンパク質に関連する特異的ｄｎａ配列並びにベクター、宿主およびワクチン
EP0597016A4 (en) * 1991-07-26 1996-04-24 Virogenetics Corp Infectious bursal disease virus recombinant poxvirus vaccine.
US5788970A (en) * 1994-03-29 1998-08-04 The University Of Maryland College Park Chimeric infectious bursal disease virus CDNA clones, expression products and vaccines based thereon

Also Published As

Publication number Publication date
CN1168500C (zh) 2004-09-29
IL128804A0 (en) 2000-01-31
NO991074L (no) 1999-04-29
NZ334667A (en) 2000-09-29
IL128804A (en) 2006-08-01
ATE460178T1 (de) 2010-03-15
BR9712012B1 (pt) 2009-08-11
HUP9904365A2 (hu) 2000-04-28
JP4670025B2 (ja) 2011-04-13
KR100475427B1 (ko) 2005-03-10
CA2264488C (en) 2009-04-07
JP2001501082A (ja) 2001-01-30
WO1998009646A1 (en) 1998-03-12
US5871744A (en) 1999-02-16
CA2264488A1 (en) 1998-03-12
EP0959901A4 (en) 2002-11-04
DE69739805D1 (de) 2010-04-22
CN1229358A (zh) 1999-09-22
KR20010029487A (ko) 2001-04-06
PL332184A1 (en) 1999-08-30
NO991074D0 (no) 1999-03-04
EP1930026A1 (en) 2008-06-11
CZ74299A3 (cs) 1999-08-11
EP1930026B1 (en) 2010-03-10
AU741055B2 (en) 2001-11-22
PL190220B1 (pl) 2005-11-30
AU3891897A (en) 1998-03-26
HUP9904365A3 (en) 2001-06-28
EP0959901A1 (en) 1999-12-01
CZ299417B6 (cs) 2008-07-16
ES2342325T3 (es) 2010-07-05

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Legal Events

Date Code Title Description
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 11/08/2009, OBSERVADAS AS CONDICOES LEGAIS.

B21F Lapse acc. art. 78, item iv - on non-payment of the annual fees in time

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B24J Lapse because of non-payment of annual fees (definitively: art 78 iv lpi, resolution 113/2013 art. 12)

Free format text: EM VIRTUDE DA EXTINCAO PUBLICADA NA RPI 2259 DE 22-04-2014 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDA A EXTINCAO DA PATENTE E SEUS CERTIFICADOS, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.