BR9608910A - Cassette de ácido nucléico processos para preparar e selecionar uma cepa mutante e não recombinante de microorganismo para determinar a identidade e a sequência de ácido nucléico do regulador de ativação de uma sequência indutível melhoradora ou ativadora e para produzir uma proteína ou polipeptídeo heterólogos ou homólogos vetor célula hospedeira sequências de ácido nucléico e de aminoácidos usos de um cassete e de uma sequência de ácido nucléico e de uma célula hospedeira fragmento de ácido nucleico combinação de dois ou mais fragmentos e mutannte de uma sequencia de aminoacidos - Google Patents

Cassette de ácido nucléico processos para preparar e selecionar uma cepa mutante e não recombinante de microorganismo para determinar a identidade e a sequência de ácido nucléico do regulador de ativação de uma sequência indutível melhoradora ou ativadora e para produzir uma proteína ou polipeptídeo heterólogos ou homólogos vetor célula hospedeira sequências de ácido nucléico e de aminoácidos usos de um cassete e de uma sequência de ácido nucléico e de uma célula hospedeira fragmento de ácido nucleico combinação de dois ou mais fragmentos e mutannte de uma sequencia de aminoacidos

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Publication number
BR9608910A
BR9608910A BR9608910A BR9608910A BR9608910A BR 9608910 A BR9608910 A BR 9608910A BR 9608910 A BR9608910 A BR 9608910A BR 9608910 A BR9608910 A BR 9608910A BR 9608910 A BR9608910 A BR 9608910A
Authority
BR
Brazil
Prior art keywords
nucleic acid
acid sequence
sequence
mutant
cassette
Prior art date
Application number
BR9608910A
Other languages
English (en)
Inventor
Leendert Hendrik De Graaff
Henriette Catharina Den Broeck
Jacob Visser
Original Assignee
Danisco Ingredients As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Ingredients As filed Critical Danisco Ingredients As
Publication of BR9608910A publication Critical patent/BR9608910A/pt

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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/38—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from Aspergillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C07K14/4705—Regulators; Modulating activity stimulating, promoting or activating activity
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01015—Polygalacturonase (3.2.1.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01037—Xylan 1,4-beta-xylosidase (3.2.1.37)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01055—Alpha-N-arabinofuranosidase (3.2.1.55)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01099—Arabinan endo-1,5-alpha-L-arabinosidase (3.2.1.99)
BR9608910A 1995-06-23 1996-06-24 Cassette de ácido nucléico processos para preparar e selecionar uma cepa mutante e não recombinante de microorganismo para determinar a identidade e a sequência de ácido nucléico do regulador de ativação de uma sequência indutível melhoradora ou ativadora e para produzir uma proteína ou polipeptídeo heterólogos ou homólogos vetor célula hospedeira sequências de ácido nucléico e de aminoácidos usos de um cassete e de uma sequência de ácido nucléico e de uma célula hospedeira fragmento de ácido nucleico combinação de dois ou mais fragmentos e mutannte de uma sequencia de aminoacidos BR9608910A (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP95201707 1995-06-23
EP95202346 1995-08-30
PCT/NL1996/000259 WO1997000962A1 (en) 1995-06-23 1996-06-24 A novel method to isolate mutants and to clone the complementing gene

Publications (1)

Publication Number Publication Date
BR9608910A true BR9608910A (pt) 1999-05-04

Family

ID=26139424

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9608910A BR9608910A (pt) 1995-06-23 1996-06-24 Cassette de ácido nucléico processos para preparar e selecionar uma cepa mutante e não recombinante de microorganismo para determinar a identidade e a sequência de ácido nucléico do regulador de ativação de uma sequência indutível melhoradora ou ativadora e para produzir uma proteína ou polipeptídeo heterólogos ou homólogos vetor célula hospedeira sequências de ácido nucléico e de aminoácidos usos de um cassete e de uma sequência de ácido nucléico e de uma célula hospedeira fragmento de ácido nucleico combinação de dois ou mais fragmentos e mutannte de uma sequencia de aminoacidos

Country Status (14)

Country Link
US (3) US6177261B1 (pt)
EP (2) EP1514936B1 (pt)
JP (2) JP4339400B2 (pt)
CN (2) CN1192106C (pt)
AT (2) ATE429499T1 (pt)
AU (1) AU714511B2 (pt)
BR (1) BR9608910A (pt)
CA (2) CA2672126C (pt)
DE (2) DE69637359T2 (pt)
DK (2) DK1514936T3 (pt)
NZ (1) NZ311181A (pt)
PL (2) PL184463B1 (pt)
PT (1) PT833922E (pt)
WO (1) WO1997000962A1 (pt)

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NZ311181A (en) * 1995-06-23 2000-02-28 Danisco Ingredients As Obtaining metabolic mutants involving random mutations and specific selection not having recombinant DNA and a nucleic acid cassette encoding a bidirectional marker, and inducible enhancer and a basic transcription unit
US6391583B1 (en) * 1998-12-18 2002-05-21 Wisconsin Alumni Research Foundation Method of producing antihypercholesterolemic agents
AU2001278496A1 (en) * 2000-07-21 2002-02-05 Syngenta Participations Ag Zinc finger domain recognition code and uses thereof
KR100428998B1 (ko) * 2001-09-10 2004-04-28 한국과학기술원 모체 단백질과는 크기와 서열이 다른 단백질의 변이집단을생산하는 방법
WO2006036678A2 (en) * 2004-09-22 2006-04-06 Bioworks, Inc. Transgenic strains of trichoderma and their use in biocontrol
GB0424940D0 (en) 2004-11-11 2004-12-15 Danisco Transcription factors
KR100801960B1 (ko) 2006-11-14 2008-02-12 건국대학교 산학협력단 ｍＲＮＡ 결합 단백질 ＸａｉＦ, 이를 부착하는 유전자구조물 그리고 이들을 이용한 외래 유전자의 과발현 방법
CN102292438A (zh) * 2008-11-03 2011-12-21 艾欧基能源公司 用于生产纤维素酶的宿主和发酵方法
US9012186B2 (en) 2009-04-27 2015-04-21 The Board Of Trustees Of The University Of Illinois Hemicellulose-degrading enzymes
KR101814588B1 (ko) * 2009-12-05 2018-01-04 아마노 엔자임 가부시키가이샤 변이 효소 및 그 용도
US8624082B2 (en) 2010-08-30 2014-01-07 Novozymes A/S Polypeptides having xylanase activity and polynucleotides encoding same
US9303074B2 (en) 2010-08-30 2016-04-05 Novoyzmes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
WO2012030811A1 (en) 2010-08-30 2012-03-08 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
US8629325B2 (en) 2010-08-30 2014-01-14 Novozymes A/S Polypeptides having beta-glucosidase activity and polynucleotides encoding same
WO2012030844A1 (en) 2010-08-30 2012-03-08 Novozymes A/S Polypeptides having endoglucanase activity and polynucleotides encoding same
WO2013159710A1 (zh) * 2012-04-25 2013-10-31 中国科学院上海生命科学研究院 一种提高拜氏梭菌木糖消耗率的方法
EP3438263A4 (en) 2016-03-31 2019-09-11 Toray Industries, Inc. TRICKODERMAPILT WITH MUTANT BXL1 GENE AND PROCESS FOR PREPARING XYLOOLIGOSACCHARIDE AND GLUCOSE THEREWITH
CN108315313A (zh) * 2018-03-21 2018-07-24 中国农业科学院饲料研究所 多聚半乳糖醛酸酶及其突变体TePG28b_N85E/S86W和应用
CN112673016A (zh) * 2018-08-31 2021-04-16 基因组股份公司 用于改善的木糖利用或改善的葡萄糖和木糖共利用的xylr突变体
CN116355881B (zh) * 2023-03-10 2024-02-23 云南师范大学 酸耐受性提高的β-木糖苷酶突变体D395G及其应用
CN116515841B (zh) * 2023-06-21 2023-09-05 中国科学院天津工业生物技术研究所 一种真菌来源的启动子元件及其应用

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US5264350A (en) 1986-08-18 1993-11-23 Institut Pasteur DNA sequence performing a function which is expressed in an over-production of extracellular proteins by various strains of bacillus, and vectors containing this sequence
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US5650298A (en) 1993-06-14 1997-07-22 Basf Aktiengesellschaft Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters
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NZ311181A (en) 1995-06-23 2000-02-28 Danisco Ingredients As Obtaining metabolic mutants involving random mutations and specific selection not having recombinant DNA and a nucleic acid cassette encoding a bidirectional marker, and inducible enhancer and a basic transcription unit

Also Published As

Publication number Publication date
JPH11507838A (ja) 1999-07-13
JP4339901B2 (ja) 2009-10-07
PL185052B1 (pl) 2003-02-28
CA2672126C (en) 2013-01-08
US6177261B1 (en) 2001-01-23
CN1192106C (zh) 2005-03-09
US6599745B1 (en) 2003-07-29
WO1997000962A1 (en) 1997-01-09
AU6244396A (en) 1997-01-22
ATE429499T1 (de) 2009-05-15
DE69637359D1 (de) 2008-01-17
EP1514936A2 (en) 2005-03-16
CA2224626C (en) 2011-11-01
ATE380242T1 (de) 2007-12-15
DE69637359T2 (de) 2008-10-30
PT833922E (pt) 2009-07-27
PL324186A1 (en) 1998-05-11
DK1514936T3 (da) 2008-04-14
EP0833922A1 (en) 1998-04-08
CN1661014B (zh) 2013-05-29
CN1207128A (zh) 1999-02-03
CA2224626A1 (en) 1997-01-09
NZ311181A (en) 2000-02-28
JP4339400B2 (ja) 2009-10-07
US8461320B2 (en) 2013-06-11
CN1661014A (zh) 2005-08-31
EP0833922B1 (en) 2009-04-22
EP1514936B1 (en) 2007-12-05
DK0833922T3 (da) 2009-08-03
EP1514936A3 (en) 2005-04-13
JP2007244387A (ja) 2007-09-27
AU714511B2 (en) 2000-01-06
PL184463B1 (pl) 2002-11-29
US20030175893A1 (en) 2003-09-18
DE69637908D1 (de) 2009-06-04
CA2672126A1 (en) 1997-01-09

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