ATE429499T1 - Neuartige methode zur isolierung von mutanten und zur klonierung der complementierenden gene - Google Patents

Neuartige methode zur isolierung von mutanten und zur klonierung der complementierenden gene

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Publication number
ATE429499T1
ATE429499T1 AT96921151T AT96921151T ATE429499T1 AT E429499 T1 ATE429499 T1 AT E429499T1 AT 96921151 T AT96921151 T AT 96921151T AT 96921151 T AT96921151 T AT 96921151T AT E429499 T1 ATE429499 T1 AT E429499T1
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AT
Austria
Prior art keywords
mutants
nucleic acid
gene
encoding
activator sequence
Prior art date
Application number
AT96921151T
Other languages
English (en)
Inventor
Graaff Leendert De
Den Broeck Henriette Van
Jacob Visser
Original Assignee
Danisco
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco filed Critical Danisco
Application granted granted Critical
Publication of ATE429499T1 publication Critical patent/ATE429499T1/de

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/38—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from Aspergillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C07K14/4705—Regulators; Modulating activity stimulating, promoting or activating activity
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01015—Polygalacturonase (3.2.1.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01037—Xylan 1,4-beta-xylosidase (3.2.1.37)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01055—Alpha-N-arabinofuranosidase (3.2.1.55)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01099—Arabinan endo-1,5-alpha-L-arabinosidase (3.2.1.99)

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  • Health & Medical Sciences (AREA)
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  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Mycology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Toxicology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
AT96921151T 1995-06-23 1996-06-24 Neuartige methode zur isolierung von mutanten und zur klonierung der complementierenden gene ATE429499T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP95201707 1995-06-23
EP95202346 1995-08-30
PCT/NL1996/000259 WO1997000962A1 (en) 1995-06-23 1996-06-24 A novel method to isolate mutants and to clone the complementing gene

Publications (1)

Publication Number Publication Date
ATE429499T1 true ATE429499T1 (de) 2009-05-15

Family

ID=26139424

Family Applications (2)

Application Number Title Priority Date Filing Date
AT96921151T ATE429499T1 (de) 1995-06-23 1996-06-24 Neuartige methode zur isolierung von mutanten und zur klonierung der complementierenden gene
AT04028288T ATE380242T1 (de) 1995-06-23 1996-06-24 Nucleinsäuresequenz xlnr des regulators xylr des xylanolytischen biosyntheseweges aus aspergillus niger

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT04028288T ATE380242T1 (de) 1995-06-23 1996-06-24 Nucleinsäuresequenz xlnr des regulators xylr des xylanolytischen biosyntheseweges aus aspergillus niger

Country Status (14)

Country Link
US (3) US6177261B1 (de)
EP (2) EP1514936B1 (de)
JP (2) JP4339400B2 (de)
CN (2) CN1661014B (de)
AT (2) ATE429499T1 (de)
AU (1) AU714511B2 (de)
BR (1) BR9608910A (de)
CA (2) CA2672126C (de)
DE (2) DE69637359T2 (de)
DK (2) DK0833922T3 (de)
NZ (1) NZ311181A (de)
PL (2) PL185052B1 (de)
PT (1) PT833922E (de)
WO (1) WO1997000962A1 (de)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT833922E (pt) 1995-06-23 2009-07-27 Danisco Novo método de isolamento de mutantes e de clonagem do gene complementar
US6391583B1 (en) * 1998-12-18 2002-05-21 Wisconsin Alumni Research Foundation Method of producing antihypercholesterolemic agents
AU2001278496A1 (en) * 2000-07-21 2002-02-05 Syngenta Participations Ag Zinc finger domain recognition code and uses thereof
KR100428998B1 (ko) * 2001-09-10 2004-04-28 한국과학기술원 모체 단백질과는 크기와 서열이 다른 단백질의 변이집단을생산하는 방법
US20090104165A1 (en) * 2004-09-22 2009-04-23 Bioworks, Inc. Transgenic strains of trichoderma and their use in biocontrol
GB0424940D0 (en) 2004-11-11 2004-12-15 Danisco Transcription factors
KR100801960B1 (ko) 2006-11-14 2008-02-12 건국대학교 산학협력단 ｍＲＮＡ 결합 단백질 ＸａｉＦ, 이를 부착하는 유전자구조물 그리고 이들을 이용한 외래 유전자의 과발현 방법
CA2742144A1 (en) * 2008-11-03 2010-06-03 Iogen Energy Corporation Hosts and fermentation processes for cellulase production
WO2010129287A2 (en) 2009-04-27 2010-11-11 The Board Of Trustees Of The University Of Illinois Hemicellulose-degrading enzymes
KR101814588B1 (ko) * 2009-12-05 2018-01-04 아마노 엔자임 가부시키가이샤 변이 효소 및 그 용도
US8624082B2 (en) 2010-08-30 2014-01-07 Novozymes A/S Polypeptides having xylanase activity and polynucleotides encoding same
EP2611914A1 (de) 2010-08-30 2013-07-10 Novozymes A/S Polypeptide mit verstärkter zelluloseabbauender wirkung und dafür kodierende polynukleotide
WO2012030811A1 (en) 2010-08-30 2012-03-08 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
US9267126B2 (en) 2010-08-30 2016-02-23 Novozymes, Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
WO2012030845A2 (en) * 2010-08-30 2012-03-08 Novozymes A/S Polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and polynucleotides encoding same
WO2013159710A1 (zh) * 2012-04-25 2013-10-31 中国科学院上海生命科学研究院 一种提高拜氏梭菌木糖消耗率的方法
JP7007645B2 (ja) 2016-03-31 2022-02-10 東レ株式会社 変異型ｂｘｌ１遺伝子を有するトリコデルマ属真菌及びそれを使用したキシロオリゴ糖とグルコースの製造方法
CN108315313A (zh) * 2018-03-21 2018-07-24 中国农业科学院饲料研究所 多聚半乳糖醛酸酶及其突变体TePG28b_N85E/S86W和应用
EP3844179A4 (de) * 2018-08-31 2022-08-31 Genomatica, Inc. Xylr-mutante zur verbesserten xylosenutzung oder zur verbesserten co-nutzung von glucose und xylose
CN116355881B (zh) * 2023-03-10 2024-02-23 云南师范大学 酸耐受性提高的β-木糖苷酶突变体D395G及其应用
CN116515841B (zh) * 2023-06-21 2023-09-05 中国科学院天津工业生物技术研究所 一种真菌来源的启动子元件及其应用

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US5028530A (en) * 1985-01-28 1991-07-02 Xoma Corporation AraB promoters and method of producing polypeptides, including cecropins, by microbiological techniques
DK160947C (da) * 1986-04-17 1991-10-21 Novo Industri As Gen-udtrykkelsessystem
US5264350A (en) * 1986-08-18 1993-11-23 Institut Pasteur DNA sequence performing a function which is expressed in an over-production of extracellular proteins by various strains of bacillus, and vectors containing this sequence
GB8807939D0 (en) * 1988-04-05 1988-05-05 Univ Brunel Regulatory cassette for expression vectors
US5464758A (en) * 1993-06-14 1995-11-07 Gossen; Manfred Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters
WO1994029442A2 (en) 1993-06-14 1994-12-22 Basf Aktiengesellschaft Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters
ES2198410T3 (es) 1993-07-23 2004-02-01 Dsm Ip Assets B.V. Cepas recombinantes de hongos filamentosos libres de gen marcador de seleccion: un metodo para obtener dichas cepas y su utilizacion.
PT833922E (pt) * 1995-06-23 2009-07-27 Danisco Novo método de isolamento de mutantes e de clonagem do gene complementar

Also Published As

Publication number Publication date
NZ311181A (en) 2000-02-28
US6177261B1 (en) 2001-01-23
CA2672126A1 (en) 1997-01-09
EP1514936A2 (de) 2005-03-16
ATE380242T1 (de) 2007-12-15
CN1661014B (zh) 2013-05-29
US8461320B2 (en) 2013-06-11
AU6244396A (en) 1997-01-22
DE69637359T2 (de) 2008-10-30
US6599745B1 (en) 2003-07-29
DK1514936T3 (da) 2008-04-14
CN1661014A (zh) 2005-08-31
PL324186A1 (en) 1998-05-11
DK0833922T3 (da) 2009-08-03
CN1192106C (zh) 2005-03-09
DE69637359D1 (de) 2008-01-17
DE69637908D1 (de) 2009-06-04
CA2224626A1 (en) 1997-01-09
EP0833922B1 (de) 2009-04-22
JPH11507838A (ja) 1999-07-13
CN1207128A (zh) 1999-02-03
WO1997000962A1 (en) 1997-01-09
PT833922E (pt) 2009-07-27
CA2672126C (en) 2013-01-08
EP1514936B1 (de) 2007-12-05
US20030175893A1 (en) 2003-09-18
JP4339400B2 (ja) 2009-10-07
PL184463B1 (pl) 2002-11-29
EP1514936A3 (de) 2005-04-13
PL185052B1 (pl) 2003-02-28
CA2224626C (en) 2011-11-01
BR9608910A (pt) 1999-05-04
AU714511B2 (en) 2000-01-06
JP4339901B2 (ja) 2009-10-07
JP2007244387A (ja) 2007-09-27
EP0833922A1 (de) 1998-04-08

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