BR112022009979A2 - Cepa bacteriana geneticamente modificada e métodos para construir uma cepa bacteriana geneticamente modificada e para produzir l-arginina - Google Patents

Cepa bacteriana geneticamente modificada e métodos para construir uma cepa bacteriana geneticamente modificada e para produzir l-arginina

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Publication number
BR112022009979A2
BR112022009979A2 BR112022009979A BR112022009979A BR112022009979A2 BR 112022009979 A2 BR112022009979 A2 BR 112022009979A2 BR 112022009979 A BR112022009979 A BR 112022009979A BR 112022009979 A BR112022009979 A BR 112022009979A BR 112022009979 A2 BR112022009979 A2 BR 112022009979A2
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arginine
genetically modified
bacterial strain
modified bacterial
construct
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BR112022009979A
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English (en)
Inventor
Xie Xixian
Jiang Shuai
Wen Chenhui
Wu Heyun
Liu Yining
Li Xuan
Tian Daoguang
Xiong Bo
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Ningxia Eppen Biotech Co Ltd
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Application filed by Ningxia Eppen Biotech Co Ltd filed Critical Ningxia Eppen Biotech Co Ltd
Publication of BR112022009979A2 publication Critical patent/BR112022009979A2/pt

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    • C12N9/1025—Acyltransferases (2.3)
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    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
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Abstract

São divulgadas bactérias geneticamente modificadas para a produção de L-arginina e um método de construção e uma aplicação das bactérias geneticamente modificadas. De acordo com o método, os genes que codificam uma carbamoil?fosfato sintetase e um gene que codifica uma enzima da via de biossíntese de L-arginina são integrados em Escherichia coli; a presente invenção analisou e reconstruiu a via sintética da arginina e o fluxo metabólico relacionado à arginina em toda a rede metabólica de aminoácidos em E. coli e, finalmente, obteve uma cepa bacteriana geneticamente modificada que possui um histórico genético claro, não carrega plasmídeos, não sofre mutagênese e é capaz de produzir L-arginina de forma estável e eficiente.
BR112022009979A 2019-12-02 2020-05-15 Cepa bacteriana geneticamente modificada e métodos para construir uma cepa bacteriana geneticamente modificada e para produzir l-arginina BR112022009979A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201911211097.XA CN110964683B (zh) 2019-12-02 2019-12-02 生产l-精氨酸的基因工程菌及其构建方法与应用
PCT/CN2020/090626 WO2021109467A1 (zh) 2019-12-02 2020-05-15 生产l-精氨酸的基因工程菌及其构建方法与应用

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US (1) US20220411833A1 (pt)
EP (1) EP4056676A4 (pt)
JP (1) JP7373661B2 (pt)
KR (1) KR20220088451A (pt)
CN (1) CN110964683B (pt)
BR (1) BR112022009979A2 (pt)
CA (1) CA3161765A1 (pt)
WO (1) WO2021109467A1 (pt)
ZA (1) ZA202205587B (pt)

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CN112280728B (zh) * 2020-11-16 2022-04-08 天津科技大学 一种生产l-瓜氨酸的基因工程菌株及其应用
CN112501095B (zh) * 2020-12-08 2023-05-26 南开大学 一种合成3-岩藻乳糖的重组大肠杆菌构建方法及其应用
CN112877271B (zh) * 2021-02-05 2023-03-14 江西师范大学 一种提高钝齿棒杆菌厌氧发酵产l-精氨酸的方法
CN114854658A (zh) * 2022-05-17 2022-08-05 江南大学 一种增强乙酸利用提高大肠杆菌发酵产l-精氨酸的方法
CN115011538B (zh) * 2022-06-14 2023-10-27 江南大学 一种生产l-瓜氨酸的大肠杆菌及其构建方法和应用
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CN110964683B (zh) 2021-08-13
EP4056676A1 (en) 2022-09-14
JP2023504236A (ja) 2023-02-02
JP7373661B2 (ja) 2023-11-02
ZA202205587B (en) 2023-01-25
CA3161765A1 (en) 2021-06-10
WO2021109467A1 (zh) 2021-06-10
EP4056676A4 (en) 2024-01-03
KR20220088451A (ko) 2022-06-27
US20220411833A1 (en) 2022-12-29
CN110964683A (zh) 2020-04-07

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