BR112021016728A2 - Bactérias imunostimulatórias projetadas para colonizar tumores, residentes em tumores células imunológicas e o microambiente tumoral - Google Patents

Bactérias imunostimulatórias projetadas para colonizar tumores, residentes em tumores células imunológicas e o microambiente tumoral

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BR112021016728A2
BR112021016728A2 BR112021016728A BR112021016728A BR112021016728A2 BR 112021016728 A2 BR112021016728 A2 BR 112021016728A2 BR 112021016728 A BR112021016728 A BR 112021016728A BR 112021016728 A BR112021016728 A BR 112021016728A BR 112021016728 A2 BR112021016728 A2 BR 112021016728A2
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bacteria
tumor
immune cells
pagp
immunostimulating
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BR112021016728A
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English (en)
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Charles Michel Iannello Alexandre
D Thanos Christopher
Haixing Kehoe
Justin Skoble
Hix Glickman Laura
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Actym Therapeutics Inc
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Application filed by Actym Therapeutics Inc filed Critical Actym Therapeutics Inc
Publication of BR112021016728A2 publication Critical patent/BR112021016728A2/pt

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Abstract

bactérias imunostimulatórias projetadas para colonizar tumores, residentes em tumores células imunológicas e o microambiente tumoral. são fornecidas bactérias imunoestimuladoras de entrega que aumentaram a colonização de tumores, o microambiente tumoral e/ou células imunes residentes no tumor e aumentaram a atividade antitumoral. as bactérias imunoestimuladoras são modificadas por deleção de genes que codificam os flagelos ou por modificação dos genes de modo que flagelos funcionais não sejam produzidos e/ou são modificados por deleção de pagp ou modificação de pagp para produzir o produto pagp inativo. como resultado, as bactérias imunoestimuladoras são flagelina "e/ou pagp '. as bactérias imunoestimuladoras opcionalmente têm modificações genômicas adicionais de modo que as bactérias são auxotróficas de adenosina e/ou purina. as bactérias opcionalmente são uma ou mais de asct, purl' e msbb '. as bactérias imunoestimuladoras, como as espécies de salmonella, são modificadas para codificar proteínas que induzem a expressão do interferon tipo i (ifn), ou que são variantes das mesmas que têm atividade aumentada para induzir a expressão do ifn tipo i, ou que são variantes das mesmas que resultam em expressão constitutiva de ifn tipo i. a bactéria pode codificar uma proteína estimuladora de genes de interferon (sting) modificada de uma espécie não humana, que tem menor atividade de sinalização de nf-¿¿ e, opcionalmente, maior atividade de sinalização de via de ifn tipo i, em comparação a sting humana. as bactérias infectam preferencialmente células imunes no microambiente tumoral, ou células imunes residentes no tumor, e/ou induzem menos morte celular em células imunes do que em outras células r. também são fornecidos métodos para inibir o crescimento ou reduzir o volume de um tumor sólido pela administração de bactérias imunoestimuladoras.
BR112021016728A 2019-02-27 2020-02-27 Bactérias imunostimulatórias projetadas para colonizar tumores, residentes em tumores células imunológicas e o microambiente tumoral BR112021016728A2 (pt)

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US201962811521P 2019-02-27 2019-02-27
US201962828990P 2019-04-03 2019-04-03
US201962934478P 2019-11-12 2019-11-12
US202062962140P 2020-01-16 2020-01-16
PCT/US2020/020240 WO2020176809A1 (en) 2019-02-27 2020-02-27 Immunostimulatory bacteria engineered to colonize tumors, tumor-resident immune cells, and the tumor microenvironment

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US (1) US20220135980A1 (pt)
EP (1) EP3931210A1 (pt)
JP (1) JP2022524951A (pt)
KR (1) KR20220004959A (pt)
CN (1) CN113748124A (pt)
AU (1) AU2020229875A1 (pt)
BR (1) BR112021016728A2 (pt)
CA (2) CA3176660A1 (pt)
IL (1) IL285608A (pt)
MX (1) MX2021010273A (pt)
SG (1) SG11202108459QA (pt)
WO (1) WO2020176809A1 (pt)

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US12024709B2 (en) 2019-02-27 2024-07-02 Actym Therapeutics, Inc. Immunostimulatory bacteria engineered to colonize tumors, tumor-resident immune cells, and the tumor microenvironment
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CN112444629B (zh) * 2020-12-09 2023-09-29 青岛科技大学 基于发夹核酸分子信号放大线路的外泌体检测方法及应用
CN112852698B (zh) * 2021-01-30 2022-11-29 军事科学院军事医学研究院军事兽医研究所 牛种布鲁氏菌A19株asd基因缺失株的构建方法和应用
KR20230165276A (ko) * 2021-03-31 2023-12-05 플래그쉽 파이어니어링 이노베이션스 브이, 인크. 타노트랜스미션 폴리펩티드 및 암의 치료에서의 이의 용도
CN113249382B (zh) * 2021-04-12 2023-05-12 右江民族医学院 下调TRIM56基因表达的siRNA及其应用
CN113549621B (zh) * 2021-07-14 2022-07-19 山西大学 一种增强细菌中外源蛋白活性和表达的最小启动子
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