BR112021009425A2 - Sistemas e métodos de diagnóstico por gotículas com base no sistema crispr - Google Patents

Sistemas e métodos de diagnóstico por gotículas com base no sistema crispr

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Publication number
BR112021009425A2
BR112021009425A2 BR112021009425A BR112021009425A BR112021009425A2 BR 112021009425 A2 BR112021009425 A2 BR 112021009425A2 BR 112021009425 A BR112021009425 A BR 112021009425A BR 112021009425 A BR112021009425 A BR 112021009425A BR 112021009425 A2 BR112021009425 A2 BR 112021009425A2
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BR
Brazil
Prior art keywords
droplet
diagnostic systems
methods based
crispr system
detection
Prior art date
Application number
BR112021009425A
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English (en)
Inventor
Cameron Myhrvold
Amanda Freije Catherine
Cheri Ackerman
Deborah Hung
Gowtham Thakku
Hayden Metsky
Jared Kehe
Pardis Sabeti
Paul Blainey
Original Assignee
Massachusetts Inst Technology
Harvard College
Broad Inst Inc
Massachusetts Gen Hospital
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Massachusetts Inst Technology, Harvard College, Broad Inst Inc, Massachusetts Gen Hospital filed Critical Massachusetts Inst Technology
Publication of BR112021009425A2 publication Critical patent/BR112021009425A2/pt

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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
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    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/1023—Microstructural devices for non-optical measurement
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
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    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
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    • C12Q2600/00—Oligonucleotides characterized by their use
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
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  • Immunology (AREA)
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  • Genetics & Genomics (AREA)
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  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Physics & Mathematics (AREA)
  • Dispersion Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Fluid Mechanics (AREA)
  • Hematology (AREA)
  • Optics & Photonics (AREA)
  • Clinical Laboratory Science (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

sistemas e métodos de diagnóstico por gotículas com base no sistema crispr. as proteínas de direcionamento de rna são utilizadas para fornecer um diagnóstico com base em crispr massivamente multiplexado por detecção em gotículas com sensibilidade atomolar. a detecção de dna e rna com níveis comparáveis de sensibilidade em volumes de nanolitro pode diferenciar alvos de não alvos com base em diferenças de par de base único, com aplicações em vários cenários na saúde humana, incluindo, por exemplo, detecção viral, tipagem de cepas bacterianas e genotipagem sensível.
BR112021009425A 2018-11-14 2019-11-14 Sistemas e métodos de diagnóstico por gotículas com base no sistema crispr BR112021009425A2 (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201862767070P 2018-11-14 2018-11-14
US201962841812P 2019-05-01 2019-05-01
US201962871056P 2019-07-05 2019-07-05
PCT/US2019/061577 WO2020102610A1 (en) 2018-11-14 2019-11-14 Crispr system based droplet diagnostic systems and methods

Publications (1)

Publication Number Publication Date
BR112021009425A2 true BR112021009425A2 (pt) 2021-11-23

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US (1) US20220073987A1 (pt)
EP (1) EP3880817A1 (pt)
JP (1) JP2022513602A (pt)
KR (1) KR20210104698A (pt)
CN (1) CN113474456A (pt)
AU (1) AU2019379160A1 (pt)
BR (1) BR112021009425A2 (pt)
CA (1) CA3119972A1 (pt)
IL (1) IL283210A (pt)
MX (1) MX2021005701A (pt)
SG (1) SG11202105083XA (pt)
WO (1) WO2020102610A1 (pt)

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