MX2021005701A - Sistema crispr a base de sistemas y métodos de diagnóstico de gotas. - Google Patents

Sistema crispr a base de sistemas y métodos de diagnóstico de gotas.

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Publication number
MX2021005701A
MX2021005701A MX2021005701A MX2021005701A MX2021005701A MX 2021005701 A MX2021005701 A MX 2021005701A MX 2021005701 A MX2021005701 A MX 2021005701A MX 2021005701 A MX2021005701 A MX 2021005701A MX 2021005701 A MX2021005701 A MX 2021005701A
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MX
Mexico
Prior art keywords
detection
methods
system based
crispr system
diagnostic systems
Prior art date
Application number
MX2021005701A
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English (en)
Inventor
Deborah Hung
Cameron Myhrvold
Catherine Amanda Freije
Hayden Metsky
Pardis Sabeti
Gowtham Thakku
Jared Kehe
Cheri Ackerman
Paul Blainey
Original Assignee
Broad Inst Inc
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Application filed by Broad Inst Inc filed Critical Broad Inst Inc
Publication of MX2021005701A publication Critical patent/MX2021005701A/es

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    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q1/701—Specific hybridization probes
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    • G—PHYSICS
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
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    • G01N21/64—Fluorescence; Phosphorescence
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/301—Endonuclease
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
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    • C12Q2600/00—Oligonucleotides characterized by their use
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks

Abstract

La presente invención se refiere proteínas dirigidas a ARN utilizadas para proporcionar un diagnóstico robusto basado en CRISPR multiplexado masivamente mediante la detección en gotas con sensibilidad atomolar. La detección de ADN y ARN con niveles comparables de sensibilidad en volúmenes de nanolitros puede diferenciar los objetivos de los no objetivos en función de las diferencias de un solo par de bases, con aplicaciones en múltiples escenarios en la salud humana que incluyen, por ejemplo, detección viral, tipificación de cepas bacterianas y sensibilidad a genotipado.
MX2021005701A 2018-11-14 2019-11-14 Sistema crispr a base de sistemas y métodos de diagnóstico de gotas. MX2021005701A (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201862767070P 2018-11-14 2018-11-14
US201962841812P 2019-05-01 2019-05-01
US201962871056P 2019-07-05 2019-07-05
PCT/US2019/061577 WO2020102610A1 (en) 2018-11-14 2019-11-14 Crispr system based droplet diagnostic systems and methods

Publications (1)

Publication Number Publication Date
MX2021005701A true MX2021005701A (es) 2021-09-23

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MX2021005701A MX2021005701A (es) 2018-11-14 2019-11-14 Sistema crispr a base de sistemas y métodos de diagnóstico de gotas.

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US (1) US20220073987A1 (es)
EP (1) EP3880817A1 (es)
JP (1) JP2022513602A (es)
KR (1) KR20210104698A (es)
CN (1) CN113474456A (es)
AU (1) AU2019379160A1 (es)
CA (1) CA3119972A1 (es)
IL (1) IL283210A (es)
MX (1) MX2021005701A (es)
SG (1) SG11202105083XA (es)
WO (1) WO2020102610A1 (es)

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CN113474456A (zh) 2021-10-01
CA3119972A1 (en) 2020-05-22
US20220073987A1 (en) 2022-03-10
JP2022513602A (ja) 2022-02-09
WO2020102610A1 (en) 2020-05-22
AU2019379160A1 (en) 2021-06-24
SG11202105083XA (en) 2021-06-29
EP3880817A1 (en) 2021-09-22
KR20210104698A (ko) 2021-08-25
IL283210A (en) 2021-06-30

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