BR112019005804A2 - processos para preparar 2-cetoácidos alongados e compostos c5-c10 dos mesmos via modificações genéticas em vias metabólicas microbianas - Google Patents

processos para preparar 2-cetoácidos alongados e compostos c5-c10 dos mesmos via modificações genéticas em vias metabólicas microbianas

Info

Publication number
BR112019005804A2
BR112019005804A2 BR112019005804A BR112019005804A BR112019005804A2 BR 112019005804 A2 BR112019005804 A2 BR 112019005804A2 BR 112019005804 A BR112019005804 A BR 112019005804A BR 112019005804 A BR112019005804 A BR 112019005804A BR 112019005804 A2 BR112019005804 A2 BR 112019005804A2
Authority
BR
Brazil
Prior art keywords
processes
compounds
leucd
metabolic pathways
genetic modifications
Prior art date
Application number
BR112019005804A
Other languages
English (en)
Inventor
Stowers Christopher
Shiue Eric
Sanghani Paresh
Bhosale Prakash
Delaplane Sarah
Greenwalt Scott
Original Assignee
Dow Global Technologies Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Global Technologies Llc filed Critical Dow Global Technologies Llc
Publication of BR112019005804A2 publication Critical patent/BR112019005804A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01085—3-Isopropylmalate dehydrogenase (1.1.1.85)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01048—Long-chain-aldehyde dehydrogenase (1.2.1.48)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/03—Acyl groups converted into alkyl on transfer (2.3.3)
    • C12Y203/03013—2-Isopropylmalate synthase (2.3.3.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • C12Y402/01033—3-Isopropylmalate dehydratase (4.2.1.33)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

complexos de enzima de leucd? geneticamente modificados, processos para preparar um c7-c11 2-cetoácido utilizando complexos de enzima de leucd? geneticamente modificados e organismos microbianos incluindo complexos de enzima de leucd modificados são descritos. os complexos de enzimas de leucd? geneticamente modificados, processos para preparar um c7-c11 2-cetoácido e organismos microbianos incluindo complexos de enzimas de leucd? modificados podem ser particularmente úteis para produzir c6-c10 aldeídos, alcanos, álcoois e ácidos carboxílicos, tanto in vivo como in vitro.
BR112019005804A 2016-09-30 2016-12-30 processos para preparar 2-cetoácidos alongados e compostos c5-c10 dos mesmos via modificações genéticas em vias metabólicas microbianas BR112019005804A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662402569P 2016-09-30 2016-09-30
US201662402586P 2016-09-30 2016-09-30
PCT/US2016/069476 WO2018063424A1 (en) 2016-09-30 2016-12-30 Processes to prepare elongated 2-ketoacids and c-5-c10 compounds therefrom via genetic modifications to microbial metabolic pathways

Publications (1)

Publication Number Publication Date
BR112019005804A2 true BR112019005804A2 (pt) 2019-06-25

Family

ID=57868364

Family Applications (2)

Application Number Title Priority Date Filing Date
BR112019006315A BR112019006315A2 (pt) 2016-09-30 2016-12-30 processos para preparar 2-cetoácidos alongados e compostos c5-c10 dos mesmos via modificações genéticas em vias metabólicas microbianas
BR112019005804A BR112019005804A2 (pt) 2016-09-30 2016-12-30 processos para preparar 2-cetoácidos alongados e compostos c5-c10 dos mesmos via modificações genéticas em vias metabólicas microbianas

Family Applications Before (1)

Application Number Title Priority Date Filing Date
BR112019006315A BR112019006315A2 (pt) 2016-09-30 2016-12-30 processos para preparar 2-cetoácidos alongados e compostos c5-c10 dos mesmos via modificações genéticas em vias metabólicas microbianas

Country Status (6)

Country Link
US (2) US11236310B2 (pt)
EP (2) EP3532624A1 (pt)
CN (2) CN110462049A (pt)
BR (2) BR112019006315A2 (pt)
CA (2) CA3038660A1 (pt)
WO (2) WO2018063423A1 (pt)

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103540559A (zh) * 2007-02-09 2014-01-29 加利福尼亚大学董事会 利用重组微生物生产生物燃料
MX2010003501A (es) 2007-10-04 2010-06-09 Bio Architecture Lab Inc Produccion de biocombustible.
JP5578767B2 (ja) 2008-01-28 2014-08-27 克己 飯田 蒸発装置
WO2010045629A2 (en) 2008-10-18 2010-04-22 The Regents Of The University Of California Production of c5-c8 alcohols using evolved enzymes and metabolically engineered microorganisms
AU2015201431B2 (en) * 2008-11-11 2016-08-04 Alimentary Health Limited Bifidobacterium longum
KR102076532B1 (ko) * 2009-06-05 2020-02-13 에보니크 오퍼레이션즈 게엠베하 2-케토 카르복실산의 제조 방법
EP2464736A4 (en) 2009-08-12 2013-07-31 Gevo Inc PATIENT LOCALIZATION OF CYTOSOLIC ISOBUTANOL FOR THE MANUFACTURE OF ISOBUTANOL
US8372610B2 (en) 2010-09-15 2013-02-12 Ls9, Inc. Production of odd chain fatty acid derivatives in recombinant microbial cells
JP2013544516A (ja) * 2010-11-03 2013-12-19 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア タンパク質性バイオマスの発酵を介した組換え微生物によるバイオ燃料および化学物質の生成
WO2012135731A2 (en) 2011-04-01 2012-10-04 The Regents Of The University Of California Alcohol production from recombinant microorganisms
US20150259710A1 (en) 2011-07-28 2015-09-17 Gevo, Inc. Decarboxylase proteins with high keto-isovalerate decarboxylase activity
US20140377857A1 (en) 2012-02-15 2014-12-25 The Regents Of The University Of California Integrated electro-bioreactor
WO2013160124A1 (en) * 2012-04-27 2013-10-31 Evonik Industries Ag Feedback-resistant alpha-isopropylmalate synthases
WO2015089127A1 (en) 2013-12-12 2015-06-18 Dow Global Technologies Llc Processes to prepare elongated 2-ketoacids and c6-c10 compounds therefrom via genetic modifications to microbial metabolic pathways
BR112017012416A2 (pt) 2014-12-10 2018-07-31 Dow Global Technologies Llc fenilpiruvato descarboxilase geneticamente modificada, processos para preparar e usos da mesma
AU2016276973B2 (en) 2015-06-10 2022-09-01 Synlogic Operating Company, Inc. Bacteria engineered to treat disorders involving the catabolism of a branched chain amino acid

Also Published As

Publication number Publication date
CA3038660A1 (en) 2018-04-05
CN109790554A (zh) 2019-05-21
WO2018063423A1 (en) 2018-04-05
EP3532624A1 (en) 2019-09-04
WO2018063424A1 (en) 2018-04-05
EP3519579A1 (en) 2019-08-07
US20190249151A1 (en) 2019-08-15
BR112019006315A2 (pt) 2019-07-30
CA3038662A1 (en) 2018-04-05
CN109790554B (zh) 2023-06-16
US10995322B2 (en) 2021-05-04
US11236310B2 (en) 2022-02-01
US20190316158A1 (en) 2019-10-17
CN110462049A (zh) 2019-11-15

Similar Documents

Publication Publication Date Title
EP3468356A4 (en) GENETICALLY MODIFIED CELLS, TISSUES AND ORGANS FOR THE TREATMENT OF A DISEASE
EP3229586A4 (en) Genetically modified cells, tissues, and organs for treating disease
MX2018010529A (es) Composiciones y metodos para la produccion y administracion de acido ribonucleico.
EA201590554A1 (ru) Ингибиторы глюкозилцерамид-синтазы
BR112013027307A2 (pt) método para tratar uma matriz de tecido; matriz de tecido acelular
MX2015011008A (es) Indicador de esterilizacion incluyendo un indicador biologico transgenico simplificado.
JP2013005803A5 (pt)
BR112015018783A8 (pt) misturas íntimas secas, composição, método para preparação de uma mistura íntima seca e método para aumentar a germinação, crescimento, metabolismo e/ou atividade enzimática de esporos bacterianos
WO2012027514A3 (en) Compositions and methods for cardiac therapy
MX2020011415A (es) Metodos de usar zscan4 para rejuvenecer celulas humanas.
CO2018003224A2 (es) Formulaciones de aminoacidos de liberacion modificada administradas oralmente
EA201690718A1 (ru) Способ обработки целлюлозосодержащей биомассы
BR112016006261A2 (pt) camundongo, célula ou tecido de camundongo isolado, e, métodos para produzir um camundongo, para enxertar células humanas em um camundongo e para avaliar a eficácia terapêutica de uma droga que alveja células humanas
MX2017003782A (es) Derivado de isoxazol como inhibidor de isocitrato deshidrogenasa 1 mutante.
BR112014032940A2 (pt) ésteres de carboidratos como indutores de expressão de genes
UY35439A (es) Formas salinas del (2-(2-(4-fluorofenil)tiazol-4-il)propan-2-il)carbamato de (S)-quinuclidin-3-ilo.
Del Monte et al. An endothelial contribution to coronary vessels
BR112019005804A2 (pt) processos para preparar 2-cetoácidos alongados e compostos c5-c10 dos mesmos via modificações genéticas em vias metabólicas microbianas
GB201212111D0 (en) Vascular bed-specific endothelial cells
PL410898A1 (pl) Sposób otrzymywania estrów kwasu mlekowego i mleczanomlekowego w reakcji alkoholizy poliestru alifatycznego
BR112017026005A2 (pt) ?polipeptídeo, polinucleotídeo, micro-organismo do gênero escherichia, método de produção de o- acetil-homosserina e de l-metionina?
WO2015041603A8 (en) Method for detection of a genetic variant
NZ720244A (en) Methods of feeding fish fermentation cell mass
WO2013102118A3 (en) Imageable patient tumors in mice
BAI et al. Investigation report of Russian soybean production status

Legal Events

Date Code Title Description
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B350 Update of information on the portal [chapter 15.35 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]