BR112019006315A2 - processos para preparar 2-cetoácidos alongados e compostos c5-c10 dos mesmos via modificações genéticas em vias metabólicas microbianas - Google Patents

processos para preparar 2-cetoácidos alongados e compostos c5-c10 dos mesmos via modificações genéticas em vias metabólicas microbianas

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Publication number
BR112019006315A2
BR112019006315A2 BR112019006315A BR112019006315A BR112019006315A2 BR 112019006315 A2 BR112019006315 A2 BR 112019006315A2 BR 112019006315 A BR112019006315 A BR 112019006315A BR 112019006315 A BR112019006315 A BR 112019006315A BR 112019006315 A2 BR112019006315 A2 BR 112019006315A2
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BR
Brazil
Prior art keywords
processes
compounds
metabolic pathways
genetic modifications
keto acids
Prior art date
Application number
BR112019006315A
Other languages
English (en)
Inventor
Sanghani Paresh
Delaplane Sarah
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Dow Global Technologies Llc
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Publication date
Application filed by Dow Global Technologies Llc filed Critical Dow Global Technologies Llc
Publication of BR112019006315A2 publication Critical patent/BR112019006315A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01085—3-Isopropylmalate dehydrogenase (1.1.1.85)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01048—Long-chain-aldehyde dehydrogenase (1.2.1.48)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/03—Acyl groups converted into alkyl on transfer (2.3.3)
    • C12Y203/03013—2-Isopropylmalate synthase (2.3.3.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • C12Y402/01033—3-Isopropylmalate dehydratase (4.2.1.33)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

isopropilmalato sintases geneticamente modificadas, processos para preparar um c7-c11 2-cetoácido utilizando isopropilmalato sintases geneticamente modificadas e organismos microbianos incluindo isopropilmalato sintases geneticamente modificadas são descritos. as isopropilmalato sintases geneticamente modificadas, os processos para preparar um c7-c11 2-cetoácido e os organismos microbianos incluindo isopropilmalato sintases geneticamente modificadas podem ser particularmente úteis para produzir cn-1 aldeídos, álcoois, ácidos carboxílicos e cn-2 alcanos tanto in vivo quanto in vitro.
BR112019006315A 2016-09-30 2016-12-30 processos para preparar 2-cetoácidos alongados e compostos c5-c10 dos mesmos via modificações genéticas em vias metabólicas microbianas BR112019006315A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662402569P 2016-09-30 2016-09-30
US201662402586P 2016-09-30 2016-09-30
PCT/US2016/069430 WO2018063423A1 (en) 2016-09-30 2016-12-30 Processes to prepare elongated 2-ketoacids and c5-c10 compounds therefrom via genetic modifications to microbial metabolic pathways

Publications (1)

Publication Number Publication Date
BR112019006315A2 true BR112019006315A2 (pt) 2019-07-30

Family

ID=57868364

Family Applications (2)

Application Number Title Priority Date Filing Date
BR112019005804A BR112019005804A2 (pt) 2016-09-30 2016-12-30 processos para preparar 2-cetoácidos alongados e compostos c5-c10 dos mesmos via modificações genéticas em vias metabólicas microbianas
BR112019006315A BR112019006315A2 (pt) 2016-09-30 2016-12-30 processos para preparar 2-cetoácidos alongados e compostos c5-c10 dos mesmos via modificações genéticas em vias metabólicas microbianas

Family Applications Before (1)

Application Number Title Priority Date Filing Date
BR112019005804A BR112019005804A2 (pt) 2016-09-30 2016-12-30 processos para preparar 2-cetoácidos alongados e compostos c5-c10 dos mesmos via modificações genéticas em vias metabólicas microbianas

Country Status (6)

Country Link
US (2) US10995322B2 (pt)
EP (2) EP3519579A1 (pt)
CN (2) CN109790554B (pt)
BR (2) BR112019005804A2 (pt)
CA (2) CA3038660A1 (pt)
WO (2) WO2018063424A1 (pt)

Family Cites Families (16)

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KR20090117739A (ko) * 2007-02-09 2009-11-12 더 리전트 오브 더 유니버시티 오브 캘리포니아 재조합 미생물을 이용한 생물연료의 생산
CL2008002960A1 (es) 2007-10-04 2009-05-08 Bio Arch Lab Inc Método para producir 2-ceto-3-desoxi d-gluconato (kdg), basado en proporciopnar acido-desoxi l-eritro hexocelulosa uronico (dehu), y contactar dicha molecula con un microorganismo recombinante que codofica una dehu-hidrogenasa.
JP5578767B2 (ja) 2008-01-28 2014-08-27 克己 飯田 蒸発装置
BRPI0919696A2 (pt) 2008-10-18 2015-08-04 Univ California Polipeptídeo substancialmente purificado, microorganismo recombinante, e, polinucleotídeo isolado.
AU2015201431B2 (en) * 2008-11-11 2016-08-04 Alimentary Health Limited Bifidobacterium longum
BRPI1012007A2 (pt) * 2009-06-05 2015-09-22 Evonik Degussa Gmbh "método para a preparação de ácido 2-ceto-carboxílico"
BR112012003241A2 (pt) 2009-08-12 2015-09-01 Gevo Inc Localização de via de isobutanol citosólico para a produção de isobutanol
US8372610B2 (en) 2010-09-15 2013-02-12 Ls9, Inc. Production of odd chain fatty acid derivatives in recombinant microbial cells
CN103314101B (zh) * 2010-11-03 2016-05-18 加利福尼亚大学董事会 通过蛋白质生物质发酵的重组微生物的生物燃料和化学生产
WO2012135731A2 (en) 2011-04-01 2012-10-04 The Regents Of The University Of California Alcohol production from recombinant microorganisms
WO2013016724A2 (en) 2011-07-28 2013-01-31 Gevo, Inc. Decarboxylase proteins with high keto-isovalerate decarboxylase activity
US20140377857A1 (en) 2012-02-15 2014-12-25 The Regents Of The University Of California Integrated electro-bioreactor
ES2666072T3 (es) * 2012-04-27 2018-04-30 Evonik Technochemie Gmbh Alfa-isopropilmalato sintasas resistentes a la retroacción
EP3080285B1 (en) * 2013-12-12 2022-07-27 Dow Global Technologies LLC Processes to prepare elongated 2-ketoacids and c6-c10 compounds therefrom via genetic modifications to microbial metabolic pathways
CA2969842C (en) 2014-12-10 2023-08-22 Dow Global Technologies Llc Genetically modified phenylpyruvate decarboxylase, processes to prepare, and uses thereof
AU2016276973B2 (en) 2015-06-10 2022-09-01 Synlogic Operating Company, Inc. Bacteria engineered to treat disorders involving the catabolism of a branched chain amino acid

Also Published As

Publication number Publication date
US20190316158A1 (en) 2019-10-17
WO2018063424A1 (en) 2018-04-05
CA3038660A1 (en) 2018-04-05
CA3038662A1 (en) 2018-04-05
EP3519579A1 (en) 2019-08-07
BR112019005804A2 (pt) 2019-06-25
US11236310B2 (en) 2022-02-01
CN110462049A (zh) 2019-11-15
EP3532624A1 (en) 2019-09-04
US10995322B2 (en) 2021-05-04
US20190249151A1 (en) 2019-08-15
WO2018063423A1 (en) 2018-04-05
CN109790554B (zh) 2023-06-16
CN109790554A (zh) 2019-05-21

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