BR112019004272A2 - método e kit para detectar micro-organismo patogênico - Google Patents

método e kit para detectar micro-organismo patogênico

Info

Publication number
BR112019004272A2
BR112019004272A2 BR112019004272A BR112019004272A BR112019004272A2 BR 112019004272 A2 BR112019004272 A2 BR 112019004272A2 BR 112019004272 A BR112019004272 A BR 112019004272A BR 112019004272 A BR112019004272 A BR 112019004272A BR 112019004272 A2 BR112019004272 A2 BR 112019004272A2
Authority
BR
Brazil
Prior art keywords
pathogenic microorganism
substance
layer section
hydrophilic solvent
reaction
Prior art date
Application number
BR112019004272A
Other languages
English (en)
Inventor
Noji Hiroyuki
Tabata Kazuhito
Original Assignee
Japan Science & Tech Agency
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Japan Science & Tech Agency filed Critical Japan Science & Tech Agency
Priority claimed from PCT/JP2017/031689 external-priority patent/WO2018043733A1/ja
Publication of BR112019004272A2 publication Critical patent/BR112019004272A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • C12Q2334/20—Coumarin derivatives
    • C12Q2334/22—4-Methylumbelliferyl, i.e. beta-methylumbelliferone, 4MU
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Food Science & Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

é fornecido um método para detectar um micro-organismo patogênico em uma amostra biológica como uma técnica que pode ser usada para detectar um micro-organismo patogênico, como o vírus da gripe com alta sensibilidade. o método inclui: uma etapa de introdução de introduzir um solvente hidrofílico que contém a amostra biológica e uma substância que serve como um substrato para uma reação envolvendo uma enzima presente em uma superfície do micro-organismo patogênico ou no micro-organismo patogênico em um espaço entre uma seção de camada inferior na qual são formados uma pluralidade de receptáculos capazes de armazenar o micro-organismo patogênico e uma seção de camada superior voltada para um lado da seção de camada inferior em cujo lado os receptáculos são formados; uma etapa de encapsulamento de introduzir um solvente hidrofóbico no espaço para formar no receptáculo uma gotícula do solvente hidrofílico que é revestida com o solvente hidrofóbico e envolve o micro-organismo patogênico e a substância; e uma etapa de detecção de detectar opticamente um produto de reação produzido pela reação entre a enzima e a substância na gotícula. o solvente hidrofílico tem um valor de ph superior a uma constante de dissociação de ácido (pka) do produto da reação.
BR112019004272A 2016-09-05 2017-09-04 método e kit para detectar micro-organismo patogênico BR112019004272A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2016172515 2016-09-05
JP2017099579A JP7016136B2 (ja) 2016-09-05 2017-05-19 病原性微生物検出のための方法及びキット
PCT/JP2017/031689 WO2018043733A1 (ja) 2016-09-05 2017-09-04 病原性微生物検出のための方法及びキット

Publications (1)

Publication Number Publication Date
BR112019004272A2 true BR112019004272A2 (pt) 2019-06-04

Family

ID=61624311

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112019004272A BR112019004272A2 (pt) 2016-09-05 2017-09-04 método e kit para detectar micro-organismo patogênico

Country Status (6)

Country Link
US (1) US11008603B2 (pt)
EP (1) EP3508586A4 (pt)
JP (1) JP7016136B2 (pt)
CN (1) CN109689882A (pt)
BR (1) BR112019004272A2 (pt)
TW (1) TW201812297A (pt)

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JP7329851B2 (ja) * 2018-03-02 2023-08-21 国立研究開発法人科学技術振興機構 酵素反応生成物の検出方法
CN113518828A (zh) * 2019-03-05 2021-10-19 国立研究开发法人科学技术振兴机构 用于流感病毒检测的方法及试剂盒、以及流感病毒感染的诊断方法
CN110643503B (zh) * 2019-10-30 2023-03-28 北京陆桥技术股份有限公司 一种高精度微生物检测芯片
CN111122527A (zh) * 2019-12-18 2020-05-08 中国科学院南海海洋研究所 一种水环境中细菌原位显微成像检测装置及检测方法
EP3865583A1 (de) * 2020-02-14 2021-08-18 Testo bioAnalytics GmbH Verfahren zum nachweis von mikroorganismen und scheibenförmiger probenträger
JP7466627B2 (ja) 2020-03-27 2024-04-12 京セラ株式会社 情報処理装置、検査システム、情報処理装置の制御方法、制御プログラム、記録媒体
US20230294094A1 (en) 2020-07-29 2023-09-21 Kyocera Corporation Flow channel device
JPWO2022202217A1 (pt) 2021-03-26 2022-09-29

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Also Published As

Publication number Publication date
JP2018038384A (ja) 2018-03-15
JP7016136B2 (ja) 2022-02-04
CN109689882A (zh) 2019-04-26
US20190194717A1 (en) 2019-06-27
TW201812297A (zh) 2018-04-01
EP3508586A4 (en) 2020-04-29
US11008603B2 (en) 2021-05-18
EP3508586A1 (en) 2019-07-10

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Legal Events

Date Code Title Description
B350 Update of information on the portal [chapter 15.35 patent gazette]
B06W Patent application suspended after preliminary examination (for patents with searches from other patent authorities) chapter 6.23 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements