BR112018069557A2 - spike-ins de ácido nucléico sintético - Google Patents

spike-ins de ácido nucléico sintético

Info

Publication number
BR112018069557A2
BR112018069557A2 BR112018069557A BR112018069557A BR112018069557A2 BR 112018069557 A2 BR112018069557 A2 BR 112018069557A2 BR 112018069557 A BR112018069557 A BR 112018069557A BR 112018069557 A BR112018069557 A BR 112018069557A BR 112018069557 A2 BR112018069557 A2 BR 112018069557A2
Authority
BR
Brazil
Prior art keywords
synthetic nucleic
ins
nucleic acid
sample
tracking
Prior art date
Application number
BR112018069557A
Other languages
English (en)
Portuguese (pt)
Inventor
C Christians Fred
D Vilfan Igor
Kertesz Michael
ROSEN Michael
Sit Rene
Venkatasubrahmanyam Shivkumar
A Blauwkamp Timothy
Original Assignee
Karius Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Karius Inc filed Critical Karius Inc
Publication of BR112018069557A2 publication Critical patent/BR112018069557A2/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1065Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06Biochemical methods, e.g. using enzymes or whole viable microorganisms
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10Sequence alignment; Homology search
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2545/00Reactions characterised by their quantitative nature
    • C12Q2545/10Reactions characterised by their quantitative nature the purpose being quantitative analysis
    • C12Q2545/101Reactions characterised by their quantitative nature the purpose being quantitative analysis with an internal standard/control
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/166Oligonucleotides used as internal standards, controls or normalisation probes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Immunology (AREA)
  • Evolutionary Biology (AREA)
  • Medical Informatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
BR112018069557A 2016-03-25 2017-03-24 spike-ins de ácido nucléico sintético BR112018069557A2 (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201662313668P 2016-03-25 2016-03-25
US201662397873P 2016-09-21 2016-09-21
US201762451363P 2017-01-27 2017-01-27
PCT/US2017/024176 WO2017165864A1 (en) 2016-03-25 2017-03-24 Synthetic nucleic acid spike-ins

Publications (1)

Publication Number Publication Date
BR112018069557A2 true BR112018069557A2 (pt) 2019-01-29

Family

ID=59897735

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112018069557A BR112018069557A2 (pt) 2016-03-25 2017-03-24 spike-ins de ácido nucléico sintético

Country Status (11)

Country Link
US (5) US9976181B2 (https=)
EP (3) EP4198146A3 (https=)
JP (4) JP6743268B2 (https=)
KR (3) KR102326769B1 (https=)
CN (2) CN109312400A (https=)
AU (4) AU2017237199B2 (https=)
BR (1) BR112018069557A2 (https=)
CA (2) CA3185611A1 (https=)
IL (2) IL260924B (https=)
MX (3) MX383493B (https=)
WO (1) WO2017165864A1 (https=)

Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2971444A1 (en) 2014-12-20 2016-06-23 Arc Bio, Llc Compositions and methods for targeted depletion, enrichment, and partitioning of nucleic acids using crispr/cas system proteins
CN107922971A (zh) 2015-05-18 2018-04-17 凯锐思公司 用于富集核酸群体的组合物和方法
AU2016308283B2 (en) 2015-08-19 2022-04-21 Arc Bio, Llc Capture of nucleic acids using a nucleic acid-guided nuclease-based system
CA3185611A1 (en) 2016-03-25 2017-09-28 Karius, Inc. Synthetic nucleic acid spike-ins
WO2018191563A1 (en) 2017-04-12 2018-10-18 Karius, Inc. Sample preparation methods, systems and compositions
EP3662480A4 (en) 2017-08-04 2021-05-19 BillionToOne, Inc. MOLECULES ASSOCIATED WITH A TARGET FOR A CHARACTERIZATION ASSOCIATED WITH BIOLOGICAL TARGETS
US11430543B2 (en) 2017-08-04 2022-08-30 Billiontoone, Inc. Sequencing output determination and analysis with target-associated molecules in quantification associated with biological targets
US11519024B2 (en) 2017-08-04 2022-12-06 Billiontoone, Inc. Homologous genomic regions for characterization associated with biological targets
CN109680343B (zh) * 2017-10-18 2022-02-18 深圳华大生命科学研究院 一种外泌体微量dna的建库方法
ES2970286T3 (es) 2018-01-05 2024-05-27 Billiontoone Inc Plantillas de control de calidad para garantizar la validez de ensayos basados en secuenciación
US11970786B2 (en) * 2018-02-15 2024-04-30 Bioo Scientific Corporation Methods and kits for detecting contamination and sample misidentification
US11268132B2 (en) 2018-02-15 2022-03-08 Thrive Earlier Detection Corp. Barcoded molecular standards
WO2019178157A1 (en) 2018-03-16 2019-09-19 Karius, Inc. Sample series to differentiate target nucleic acids from contaminant nucleic acids
GB201805676D0 (en) * 2018-04-05 2018-05-23 Imperial Innovations Ltd Compositions
WO2019226648A1 (en) 2018-05-21 2019-11-28 Battelle Memorial Institute Methods and control compositions for sequencing and chemical analyses
AU2019291907A1 (en) 2018-06-29 2021-02-18 Grail, Inc. Nucleic acid rearrangement and integration analysis
DE102018213027A1 (de) * 2018-08-03 2020-02-06 Robert Bosch Gmbh Reaktionsgemisch, Verfahren und Kit zur Durchführung einer quantitativen Echtzeit-PCR
US12071651B2 (en) 2018-08-06 2024-08-27 Billiontoone, Inc. Dilution tagging for quantification of biological targets
IL281982B2 (en) * 2018-10-04 2025-12-01 Arc Bio Llc Normalization controls for managing low sample inputs in next generation sequencing
EP4524263A3 (en) * 2018-10-31 2025-05-28 Guardant Health, Inc. Methods, compositions and systems for calibrating epigenetic partitioning assays
CA3118742A1 (en) * 2018-11-21 2020-05-28 Karius, Inc. Detection and prediction of infectious disease
ES3059710T3 (en) 2018-11-21 2026-03-23 Karius Inc Direct-to-library methods, systems, and compositions
WO2020124003A1 (en) * 2018-12-13 2020-06-18 Battelle Memorial Institute Methods and control compositions for a quantitative polymerase chain reaction
SG11202104701XA (en) * 2018-12-20 2021-06-29 Guardant Health Inc Methods, compositions, and systems for improving recovery of nucleic acid molecules
US20230304084A1 (en) * 2019-02-28 2023-09-28 Inivata Ltd. Method for quantifying the amount of a target sequence in a sample
US20220364156A1 (en) * 2019-06-28 2022-11-17 Biota Technology, Inc. Estimating a quantity of molecules in a sample
US20220292363A1 (en) * 2019-09-02 2022-09-15 Phil Rivers Technology, Ltd. Method for automatically determining disease type and electronic apparatus
EP4045681B1 (en) * 2019-10-16 2025-07-16 Stilla Technologies Determination of nucleic acid sequence concentrations
BR112022008714A2 (pt) * 2019-11-06 2022-07-19 Univ Health Network Controles de picos sintéticos para sequenciamento medip livre de células e métodos de uso dos mesmos
CN111599409B (zh) * 2020-05-20 2022-05-20 电子科技大学 基于MapReduce并行的circRNA识别方法
US20230203573A1 (en) * 2020-05-29 2023-06-29 Natera, Inc. Methods for detection of donor-derived cell-free dna
KR20220045451A (ko) 2020-10-05 2022-04-12 주식회사 엘지에너지솔루션 배터리 상태 진단 장치 및 방법
CN112266978A (zh) * 2020-10-22 2021-01-26 深圳国际旅行卫生保健中心(深圳海关口岸门诊部) 引物探针组合、检测试剂盒及其应用
US20220195496A1 (en) * 2020-12-17 2022-06-23 Karius, Inc. Sequencing microbial cell-free dna from asymptomatic individuals
EP4263866A4 (en) * 2020-12-21 2024-12-04 Karius, Inc. Sequencing microbial cell-free nucleic acids to detect inflammation, secondary infection, and disease severity
US12545952B2 (en) * 2021-03-01 2026-02-10 The Broad Institute, Inc. Amplicon-based sequencing using dna spike-ins
CN115109840B (zh) * 2021-03-18 2025-08-12 厦门大学 一种分析目标核酸中突变的存在及其类型的方法
KR20230163434A (ko) * 2021-03-29 2023-11-30 일루미나, 인코포레이티드 라이브러리에서 dna 손상을 평가하고 앰플리콘 크기 바이어스를 정규화하기 위한 조성물 및 방법
CN118696132A (zh) * 2021-12-22 2024-09-24 奥科坦特公司 用于报道基因筛选测定的质量控制
EP4457358A1 (en) 2021-12-30 2024-11-06 SomaLogic Operating Co., Inc. Next-generation sequencing for protein measurement
WO2023164539A2 (en) * 2022-02-25 2023-08-31 Eclipse Bioinnovations, Inc. Methods for oligo targeted proximity ligation
JP2025509878A (ja) * 2022-03-21 2025-04-11 ビリオントゥーワン,インコーポレイテッド 処置モニタリングのためのメチル化セルフリーdnaの分子計数
JP2024100144A (ja) 2023-01-13 2024-07-26 国立研究開発法人産業技術総合研究所 ゲノムまたはメタゲノム解析のための内部標準核酸
AU2024257509A1 (en) 2023-04-18 2025-10-30 Karius, Inc. Sample processing to enrich for select species of nucleic acids
CN117008647A (zh) * 2023-06-15 2023-11-07 深圳赛陆医疗科技有限公司 控制方法、基因测序仪和存储介质
WO2025160484A1 (en) 2024-01-25 2025-07-31 Karius, Inc. Microbial and human cell-free dna biomarkers for diagnosing and assessing the severity of inflammatory bowel disease

Family Cites Families (119)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6969488B2 (en) 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
USRE39920E1 (en) 1997-05-30 2007-11-13 Xenomics, Inc. Methods for detection of nucleic acid sequences in urine
US20030022207A1 (en) 1998-10-16 2003-01-30 Solexa, Ltd. Arrayed polynucleotides and their use in genome analysis
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
US20040106110A1 (en) 1998-07-30 2004-06-03 Solexa, Ltd. Preparation of polynucleotide arrays
US7211390B2 (en) 1999-09-16 2007-05-01 454 Life Sciences Corporation Method of sequencing a nucleic acid
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
WO2001023610A2 (en) 1999-09-29 2001-04-05 Solexa Ltd. Polynucleotide sequencing
GB0002389D0 (en) 2000-02-02 2000-03-22 Solexa Ltd Molecular arrays
US20040005555A1 (en) * 2000-08-31 2004-01-08 Rothman Richard E. Molecular diagnosis of bactermia
AU2001293163A1 (en) 2000-09-27 2002-04-08 Lynx Therapeutics, Inc. Method for determining relative abundance of nucleic acid sequences
US6753137B2 (en) 2001-01-31 2004-06-22 The Chinese University Of Hong Kong Circulating epstein-barr virus DNA in the serum of patients with gastric carcinoma
CA2451985C (en) * 2001-07-19 2012-03-13 Infectio Diagnostic (I.D.I.) Inc. Universal method and composition for the rapid lysis of cells for the release of nucleic acids and their detection
JP2003101204A (ja) 2001-09-25 2003-04-04 Nec Kansai Ltd 配線基板及び配線基板の製造方法並びに電子部品
US20050124022A1 (en) 2001-10-30 2005-06-09 Maithreyan Srinivasan Novel sulfurylase-luciferase fusion proteins and thermostable sulfurylase
US6902921B2 (en) 2001-10-30 2005-06-07 454 Corporation Sulfurylase-luciferase fusion proteins and thermostable sulfurylase
US20050202414A1 (en) 2001-11-15 2005-09-15 The Regents Of The University Of California Apparatus and methods for detecting a microbe in a sample
WO2003052101A1 (en) * 2001-12-14 2003-06-26 Rosetta Inpharmatics, Inc. Sample tracking using molecular barcodes
WO2005003375A2 (en) 2003-01-29 2005-01-13 454 Corporation Methods of amplifying and sequencing nucleic acids
US20040209299A1 (en) 2003-03-07 2004-10-21 Rubicon Genomics, Inc. In vitro DNA immortalization and whole genome amplification using libraries generated from randomly fragmented DNA
US7108979B2 (en) * 2003-09-03 2006-09-19 Agilent Technologies, Inc. Methods to detect cross-contamination between samples contacted with a multi-array substrate
US20070111233A1 (en) 2003-10-30 2007-05-17 Bianchi Diana W Prenatal diagnosis using cell-free fetal DNA in amniotic fluid
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US20060024711A1 (en) 2004-07-02 2006-02-02 Helicos Biosciences Corporation Methods for nucleic acid amplification and sequence determination
US20060012793A1 (en) 2004-07-19 2006-01-19 Helicos Biosciences Corporation Apparatus and methods for analyzing samples
US7276720B2 (en) 2004-07-19 2007-10-02 Helicos Biosciences Corporation Apparatus and methods for analyzing samples
US20060024678A1 (en) 2004-07-28 2006-02-02 Helicos Biosciences Corporation Use of single-stranded nucleic acid binding proteins in sequencing
US20060073506A1 (en) * 2004-09-17 2006-04-06 Affymetrix, Inc. Methods for identifying biological samples
WO2006089203A2 (en) 2005-02-17 2006-08-24 Istituto Nazionale Per Le Malattie Infettive Irccs Lazzaro Spallanzani Compositions and methods for detecting viral specific nucleic acids in urine
AU2006214444B8 (en) 2005-02-17 2012-04-05 Trovagene, Inc. Compositions and methods for detecting pathogen specific nucleic acids in urine
ITRM20050068A1 (it) 2005-02-17 2006-08-18 Istituto Naz Per Le Malattie I Metodo per la rivelazione di acidi nucleici di agenti patogeni batterici o di parassiti nelle urine.
ITRM20050067A1 (it) 2005-02-17 2006-08-18 Istituto Naz Per Le Malattie I Metodo per la rivelazione di acidi nucleici virali o di origine virale nelle urine.
US7914982B2 (en) 2005-02-17 2011-03-29 Trovagene, Inc. Methods for detecting pathogen specific nucleic acids in urine
US8785130B2 (en) * 2005-07-07 2014-07-22 Bio-Id Diagnostic Inc. Use of markers including nucleotide sequence based codes to monitor methods of detection and identification of genetic material
US8532930B2 (en) 2005-11-26 2013-09-10 Natera, Inc. Method for determining the number of copies of a chromosome in the genome of a target individual using genetic data from genetically related individuals
AT504194B1 (de) * 2006-09-07 2008-07-15 Oesterr Rotes Kreuz Bakteriennachweis
US8101353B2 (en) 2007-12-18 2012-01-24 Advanced Analytical Technologies, Inc. System and method for nucleotide sequence profiling for sample identification
WO2009099602A1 (en) * 2008-02-04 2009-08-13 Massachusetts Institute Of Technology Selection of nucleic acids by solution hybridization to oligonucleotide baits
US20110160290A1 (en) * 2008-05-21 2011-06-30 Muneesh Tewari Use of extracellular rna to measure disease
ES2595489T3 (es) 2008-07-18 2016-12-30 Trovagene, Inc. Métodos para la detección de secuencias de ácidos nucleicos "ultracortos" basados en PCR
US20100062494A1 (en) * 2008-08-08 2010-03-11 President And Fellows Of Harvard College Enzymatic oligonucleotide pre-adenylation
HUE031848T2 (en) 2008-09-20 2017-08-28 Univ Leland Stanford Junior Non-invasive diagnosis of fetal aneuploidy by sequencing
US20120190663A1 (en) 2009-08-06 2012-07-26 Cedars-Sinai Medical Center Use of free dna as an early predictor of severity in acute pancreatitis
HUE052213T2 (hu) 2009-11-06 2021-04-28 Univ Leland Stanford Junior Grafitkilökõdés nem invazív diagnosztizálása szervátültetett betegekben
US8835358B2 (en) * 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
JP2013531983A (ja) 2010-06-11 2013-08-15 パソジェニカ,インコーポレイテッド 多重生物検出のための核酸ならびにその使用および製造方法
CN103069006A (zh) 2010-07-23 2013-04-24 艾索特里克斯遗传实验室有限责任公司 区别表达的胚胎或母源基因组区的鉴定及其用途
US20120077185A1 (en) 2010-08-06 2012-03-29 Tandem Diagnostics, Inc. Detection of genetic abnormalities and infectious disease
CA2811185C (en) * 2010-09-21 2020-09-22 Population Genetics Technologies Ltd. Increasing confidence of allele calls with molecular counting
WO2012078792A2 (en) 2010-12-07 2012-06-14 Stanford University Non-invasive determination of fetal inheritance of parental haplotypes at the genome-wide scale
US9260753B2 (en) 2011-03-24 2016-02-16 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
AU2012236109A1 (en) 2011-04-01 2013-11-21 Occam Biolabs, Inc. Methods and kits for detecting cell-free pathogen-specific nucleic acids
US9809904B2 (en) * 2011-04-21 2017-11-07 University Of Washington Through Its Center For Commercialization Methods for retrieval of sequence-verified DNA constructs
US20140179726A1 (en) 2011-05-19 2014-06-26 Virginia Commonwealth University Gut microflora as biomarkers for the prognosis of cirrhosis and brain dysfunction
SG10201605049QA (en) * 2011-05-20 2016-07-28 Fluidigm Corp Nucleic acid encoding reactions
EP2718862B1 (en) 2011-06-06 2018-10-31 Koninklijke Philips N.V. Method for assembly of nucleic acid sequence data
BR112014000138B1 (pt) 2011-07-06 2021-11-16 Quest Diagnostics Investments Incorporation Método para identificar a presença ou ausência de um ácido nucleico alvo
US20130024127A1 (en) 2011-07-19 2013-01-24 John Stuelpnagel Determination of source contributions using binomial probability calculations
EP2758538B1 (en) 2011-09-22 2018-10-31 Lineage Biosciences, Inc. Methods for analyzing heterogeneous samples
KR101272017B1 (ko) * 2011-09-23 2013-06-07 주식회사 랩 지노믹스 비뇨생식기 감염 질환 진단용 dna칩
CA2850785C (en) 2011-10-06 2022-12-13 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
EP2788506A2 (en) * 2011-11-01 2014-10-15 Pathogenica, Inc. Method and system for detection of an organism
WO2013109981A1 (en) 2012-01-20 2013-07-25 Sequenom, Inc. Diagnostic processes that factor experimental conditions
WO2013113012A2 (en) 2012-01-27 2013-08-01 The Board Of Trustees Of The Leland Stanford Junior University Methods for profiling and quantitating cell-free rna
DK2814959T3 (en) * 2012-02-17 2018-04-23 Hutchinson Fred Cancer Res COMPOSITIONS AND PROCEDURES FOR EXACTLY IDENTIFYING MUTATIONS
US9892230B2 (en) 2012-03-08 2018-02-13 The Chinese University Of Hong Kong Size-based analysis of fetal or tumor DNA fraction in plasma
JP6411995B2 (ja) 2012-03-13 2018-10-24 ザ チャイニーズ ユニバーシティー オブ ホンコン 非侵襲的出生前診断のために大量並列シークエンシング・データを分析する方法
AU2013249012B2 (en) 2012-04-19 2019-03-28 The Medical College Of Wisconsin, Inc. Highly sensitive surveillance using detection of cell free DNA
EP2653562A1 (en) 2012-04-20 2013-10-23 Institut Pasteur Anellovirus genome quantification as a biomarker of immune suppression
WO2013188846A1 (en) 2012-06-15 2013-12-19 Harry Stylli Methods of detecting diseases or conditions
US20140066317A1 (en) 2012-09-04 2014-03-06 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
GB2533006B (en) 2012-09-04 2017-06-07 Guardant Health Inc Systems and methods to detect copy number variation
US10150996B2 (en) * 2012-10-19 2018-12-11 Adaptive Biotechnologies Corp. Quantification of adaptive immune cell genomes in a complex mixture of cells
EP2728014B1 (en) 2012-10-31 2015-10-07 Genesupport SA Non-invasive method for detecting a fetal chromosomal aneuploidy
CA2949622C (en) * 2012-11-26 2019-07-02 The University Of Toledo Methods for standardized sequencing of nucleic acids and uses thereof
WO2014127484A1 (en) * 2013-02-21 2014-08-28 British Columbia Cancer Agency Branch Spike-in control nucleic acids for sample tracking
US20140242582A1 (en) 2013-02-28 2014-08-28 Ariosa Diagnostics, Inc. Detection of genetic abnormalities using ligation-based detection and digital pcr
US20140285326A1 (en) 2013-03-15 2014-09-25 Aliphcom Combination speaker and light source responsive to state(s) of an organism based on sensor data
US10017807B2 (en) 2013-03-15 2018-07-10 Verinata Health, Inc. Generating cell-free DNA libraries directly from blood
EP3533884A1 (en) * 2013-03-15 2019-09-04 Ibis Biosciences, Inc. Dna sequences to assess contamination in dna sequencing
GB2528205B (en) 2013-03-15 2020-06-03 Guardant Health Inc Systems and methods to detect rare mutations and copy number variation
PL2981921T3 (pl) 2013-04-03 2023-05-08 Sequenom, Inc. Metody i procesy nieinwazyjnej oceny zmienności genetycznych
US9422551B2 (en) * 2013-05-29 2016-08-23 New England Biolabs, Inc. Adapters for ligation to RNA in an RNA library with reduced bias
WO2015013465A2 (en) * 2013-07-25 2015-01-29 Dch Molecular Diagnostics, Inc. Methods and compositions for detecting bacterial contamination
JP7451070B2 (ja) 2013-11-07 2024-03-18 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー ヒトミクロビオームおよびその成分の分析のための無細胞核酸
CN105705515B (zh) * 2013-11-07 2021-05-25 安捷伦科技有限公司 多种用于dna操作的转座酶适体
WO2015070187A2 (en) * 2013-11-11 2015-05-14 The Translational Genomics Research Institute Systems and methods for universal tail-based indexing strategies for amplicon sequencing
US10364465B2 (en) * 2013-11-12 2019-07-30 Life Technologies Corporation Reagents and methods for sequencing
WO2015085105A1 (en) 2013-12-04 2015-06-11 University Of Alaska Fairbanks Methods and compositions for enriching non-host sequences in host samples
CN104946737B (zh) 2013-12-11 2019-02-22 安可济控股有限公司 用于检测罕见序列变体的组合物和方法
EP3483287A1 (en) * 2014-01-28 2019-05-15 QIAGEN GmbH Internal amplification control
CN106103713B (zh) 2014-02-03 2021-05-28 赛默飞世尔科技波罗的海封闭股份公司 用于经控制dna片段化的方法
GB201402249D0 (en) * 2014-02-10 2014-03-26 Vela Operations Pte Ltd NGS systems control and methods involving the same
GB201405226D0 (en) 2014-03-24 2014-05-07 Cambridge Entpr Ltd Nucleic acid preparation method
GB201411603D0 (en) * 2014-06-30 2014-08-13 Vela Operations Pte Ltd Compositions for quantitative and/or semiquantitative mutation detection methods
US10450562B2 (en) * 2014-09-09 2019-10-22 Igenomx International Genomics Corporation Methods and compositions for rapid nucleic acid library preparation
KR102893385B1 (ko) 2014-12-16 2025-12-02 가반 인스티튜트 오브 메디컬 리서치 시퀀싱 컨트롤
CN107922971A (zh) 2015-05-18 2018-04-17 凯锐思公司 用于富集核酸群体的组合物和方法
WO2017027835A1 (en) 2015-08-12 2017-02-16 Circulogene Theranostics, Llc Method of preparing cell free nucleic acid molecules by in situ amplification
CN105112569B (zh) * 2015-09-14 2017-11-21 中国医学科学院病原生物学研究所 基于宏基因组学的病毒感染检测及鉴定方法
EP3405573A4 (en) * 2016-01-22 2019-09-18 Grail, Inc. METHOD AND SYSTEMS FOR HIGH-FIDELITY SEQUENCING
CA3185611A1 (en) 2016-03-25 2017-09-28 Karius, Inc. Synthetic nucleic acid spike-ins
EP3246412A1 (en) * 2016-05-17 2017-11-22 DName-iT NV Methods for identification of samples
KR102610098B1 (ko) 2016-07-06 2023-12-04 가던트 헬쓰, 인크. 무세포 핵산의 프래그멘톰 프로파일링을 위한 방법
BR112019002679A2 (pt) 2016-08-08 2019-05-14 Karius, Inc. redução de sinal de ácidos nucleicos contaminantes
WO2018045359A1 (en) 2016-09-02 2018-03-08 Karius, Inc. Detection and treatment of infection during pregnancy
TWI797095B (zh) 2016-10-24 2023-04-01 美商格瑞爾有限責任公司 腫瘤檢測之方法及系統
WO2018187521A2 (en) 2017-04-06 2018-10-11 Cornell University Methods of detecting cell-free dna in biological samples
WO2018191563A1 (en) 2017-04-12 2018-10-18 Karius, Inc. Sample preparation methods, systems and compositions
WO2018232598A1 (zh) * 2017-06-20 2018-12-27 深圳华大智造科技有限公司 Pcr引物对及其应用
US11254980B1 (en) * 2017-11-29 2022-02-22 Adaptive Biotechnologies Corporation Methods of profiling targeted polynucleotides while mitigating sequencing depth requirements
WO2019178157A1 (en) 2018-03-16 2019-09-19 Karius, Inc. Sample series to differentiate target nucleic acids from contaminant nucleic acids
CA3118742A1 (en) 2018-11-21 2020-05-28 Karius, Inc. Detection and prediction of infectious disease
ES3059710T3 (en) 2018-11-21 2026-03-23 Karius Inc Direct-to-library methods, systems, and compositions
US20220195496A1 (en) 2020-12-17 2022-06-23 Karius, Inc. Sequencing microbial cell-free dna from asymptomatic individuals
EP4263866A4 (en) 2020-12-21 2024-12-04 Karius, Inc. Sequencing microbial cell-free nucleic acids to detect inflammation, secondary infection, and disease severity
EP4274913A4 (en) 2021-01-11 2024-12-11 Karius Inc. RAPID NON-INVASIVE DETECTION AND SERIAL MONITORING OF INFECTIONS IN SUBJECTS USING MICROBIAL CELL-FREE DNA SEQUENCING
CN117897504A (zh) 2021-02-12 2024-04-16 凯锐思公司 患有莱姆病的对象中的微生物无细胞核酸的宏基因组下一代测序

Also Published As

Publication number Publication date
MX383493B (es) 2025-03-14
KR20210138154A (ko) 2021-11-18
US12595509B2 (en) 2026-04-07
CA3185611A1 (en) 2017-09-28
JP2019509068A (ja) 2019-04-04
CN116987777A (zh) 2023-11-03
JP6743268B2 (ja) 2020-08-19
WO2017165864A1 (en) 2017-09-28
IL278000A (en) 2020-11-30
US20170275691A1 (en) 2017-09-28
AU2021200637B2 (en) 2021-05-13
US11692224B2 (en) 2023-07-04
CA3014070C (en) 2023-03-14
CA3014070A1 (en) 2017-09-28
EP3433382B1 (en) 2021-09-01
EP3978627A1 (en) 2022-04-06
AU2021200637A1 (en) 2021-03-04
KR20200128194A (ko) 2020-11-11
MX2018011429A (es) 2019-06-06
US20240043923A1 (en) 2024-02-08
MX2021006899A (es) 2021-07-07
MX2021006911A (es) 2021-07-07
JP7091397B2 (ja) 2022-06-27
US9976181B2 (en) 2018-05-22
AU2024204822A1 (en) 2024-09-12
US20210324467A1 (en) 2021-10-21
JP2025060811A (ja) 2025-04-10
US11078532B2 (en) 2021-08-03
US20250034639A1 (en) 2025-01-30
IL260924B (en) 2021-01-31
AU2017237199A1 (en) 2018-08-16
CN109312400A (zh) 2019-02-05
IL278000B (en) 2022-04-01
AU2017237199B2 (en) 2020-11-05
KR102531487B1 (ko) 2023-05-10
EP4198146A3 (en) 2023-08-23
KR102175718B1 (ko) 2020-11-06
EP4198146A2 (en) 2023-06-21
KR102326769B1 (ko) 2021-11-17
JP2020182491A (ja) 2020-11-12
EP3433382A1 (en) 2019-01-30
US20180237851A1 (en) 2018-08-23
AU2021215106A1 (en) 2021-08-26
KR20180129844A (ko) 2018-12-05
EP3433382A4 (en) 2019-10-02
JP2022120165A (ja) 2022-08-17

Similar Documents

Publication Publication Date Title
BR112018069557A2 (pt) spike-ins de ácido nucléico sintético
BR112017017025A2 (pt) métodos e composições para analizar componentes celulares
MX2016016904A (es) Analisis de secuencias de acidos nucleicos.
BR112016006312A2 (pt) método e kit para determinar a presença ou ausência de staphylococcus aureus resistente à meticilina em uma amostra biológica
BR112014030298A8 (pt) Métodos e kits destinados para ensaios baseados em aptâmeros multiplexados
EA201692157A1 (ru) Синтез двухцепочечных нуклеиновых кислот
BR112017008082A2 (pt) composições e métodos para detectar um vírus de rna
MY198335A (en) Urinalysis Device and Dry Reagent for Quantitative Urinalysis
BR112016017155A2 (pt) Sistema e dispositivo para geração com alto rendimento de gotículas combinatoriais e métodos de uso
BR112015023769A2 (pt) sistemas de instrumento analítico
MX2017001134A (es) Composiciones y metodos para la deteccion y analisis de tuberculosis micobacteriana.
GB2542576A (en) Method and kit of detecting the absence of micro-oranisms
CL2017001929A1 (es) Método y producto
BR112014014307A2 (pt) métodos e kits para detecção in situ à temperatura ambiente de um ácido nucleico alvo em uma amostra biológica
TR201819546T4 (tr) Numune tür kökeni için tespit yöntemleri.
BR112016025349A2 (pt) composições e métodos para a detecção de huanglongbing
BR112016024730A2 (pt) dispositivo, método e conjunto de análise imunocromatográfica
EA201590455A1 (ru) Способ анализа нуклеиновой кислоты-мишени, набор и анализатор
BR112019005392A2 (pt) composições de hemicelulose aglomerada, métodos de preparação das mesmas e processos para enriquecer um mineral desejado a partir de um minério
BR112016015537A2 (pt) métodos e kits para detectar a presença de sequência polinucleotídea da e. histolytica em amostra
BR112022008217A2 (pt) Métodos e dispositivos para sequenciamento
BR112019007121A2 (pt) cartucho, sistema de análise e método para testagem de uma amostra
GB2541596A (en) Method and composition for determining specific antibody responses to species of filovirus
BR112019006847A2 (pt) método e sistema de análise para testagem de uma amostra
BR112019007117A2 (pt) cartucho e sistema de análise para testagem de uma amostra

Legal Events

Date Code Title Description
B350 Update of information on the portal [chapter 15.35 patent gazette]
B06W Patent application suspended after preliminary examination (for patents with searches from other patent authorities) chapter 6.23 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements