BR112018013385A2 - hidrólise enzimática parcial de triacilgliceróis - Google Patents

hidrólise enzimática parcial de triacilgliceróis

Info

Publication number
BR112018013385A2
BR112018013385A2 BR112018013385A BR112018013385A BR112018013385A2 BR 112018013385 A2 BR112018013385 A2 BR 112018013385A2 BR 112018013385 A BR112018013385 A BR 112018013385A BR 112018013385 A BR112018013385 A BR 112018013385A BR 112018013385 A2 BR112018013385 A2 BR 112018013385A2
Authority
BR
Brazil
Prior art keywords
triglycerides
enzymatic hydrolysis
host cells
partial enzymatic
lipase
Prior art date
Application number
BR112018013385A
Other languages
English (en)
Inventor
Schurmann Martin
Mueller Monika
Mugford Paul
Original Assignee
Dsm Ip Assets Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Publication of BR112018013385A2 publication Critical patent/BR112018013385A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11C—FATTY ACIDS FROM FATS, OILS OR WAXES; CANDLES; FATS, OILS OR FATTY ACIDS BY CHEMICAL MODIFICATION OF FATS, OILS, OR FATTY ACIDS OBTAINED THEREFROM
    • C11C1/00—Preparation of fatty acids from fats, fatty oils, or waxes; Refining the fatty acids
    • C11C1/02—Preparation of fatty acids from fats, fatty oils, or waxes; Refining the fatty acids from fats or fatty oils
    • C11C1/04—Preparation of fatty acids from fats, fatty oils, or waxes; Refining the fatty acids from fats or fatty oils by hydrolysis
    • C11C1/045—Preparation of fatty acids from fats, fatty oils, or waxes; Refining the fatty acids from fats or fatty oils by hydrolysis using enzymes or microorganisms, living or dead
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

são aqui divulgadas células hospedeiras que expressam um polipeptídeo, em que o polipeptídeo hidrolisa uma ligação éster de um triacilglicerol num óleo compreendendo pelo menos um ácido gordo poliinsaturado de cadeia longa, métodos para utilizar essas células hospedeiras e processos para a produção de uma lipase utilizando essas células hospedeiras.
BR112018013385A 2015-12-30 2016-12-29 hidrólise enzimática parcial de triacilgliceróis BR112018013385A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562272829P 2015-12-30 2015-12-30
PCT/IB2016/058086 WO2017115322A2 (en) 2015-12-30 2016-12-29 Partial enzymatic hydrolysis of triacylglycerols

Publications (1)

Publication Number Publication Date
BR112018013385A2 true BR112018013385A2 (pt) 2018-12-04

Family

ID=57838436

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112018013385A BR112018013385A2 (pt) 2015-12-30 2016-12-29 hidrólise enzimática parcial de triacilgliceróis

Country Status (9)

Country Link
US (1) US11535875B2 (pt)
EP (1) EP3397771A2 (pt)
JP (2) JP2019500042A (pt)
KR (1) KR20180098386A (pt)
CN (2) CN116064263A (pt)
BR (1) BR112018013385A2 (pt)
CA (1) CA3009871A1 (pt)
PE (1) PE20181403A1 (pt)
WO (1) WO2017115322A2 (pt)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20230042107A (ko) 2020-07-27 2023-03-27 디에스엠 아이피 어셋츠 비.브이. 지방산 레티닐 에스터 형성의 감소
EP4284905A1 (en) * 2021-01-28 2023-12-06 Novozymes A/S Lipase with low malodor generation
WO2023275212A1 (en) * 2021-07-01 2023-01-05 Dsm Ip Assets B.V. Sustainable production of retinyl fatty esters

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2679920A1 (fr) 1991-08-02 1993-02-05 Rhone Poulenc Rorer Sa Levures recombinantes hautement stables pour la production de proteines recombinantes, leur preparation et leur utilisation.
EP0635574B1 (en) 1993-07-23 2003-04-23 Dsm N.V. Selection marker gene free recombinant strains, a method for obtaining them and the use of these strains
WO1998046772A2 (en) 1997-04-11 1998-10-22 Dsm N.V. Gene conversion as a tool for the construction of recombinant industrial filamentous fungi
RU2230790C2 (ru) 1998-05-19 2004-06-20 Дсм Н.В. Способ расширения 5-членного кольца соединения бета-лактама до 6-членного цефема
WO2000037671A2 (en) 1998-12-22 2000-06-29 Dsm N.V. Improved in vivo production of cephalosporins
EP1130100A1 (en) 2000-02-14 2001-09-05 Unilever N.V. Modified lipolytic enzymes and their use
CN100336906C (zh) 2001-01-15 2007-09-12 广州市绿巨人生物环保技术有限公司 脂肪酶基因序列及其在酵母中的应用
ATE512979T1 (de) * 2002-02-19 2011-07-15 Procter & Gamble Neue lipase aus pilzen
US20140335580A1 (en) * 2011-09-21 2014-11-13 Board Of Supervisors Of Lousiana State University And Agricultural And Mechanical College Method for Enrichment of Eicosapentaenoic Acid and Docosahexaenoic Acid in Source Oils
WO2015087833A1 (ja) 2013-12-10 2015-06-18 天野エンザイム株式会社 改変型リパーゼ及びその用途
CN104726477B (zh) * 2013-12-23 2018-02-13 中国农业大学 一种脂肪酶编码基因及其工程菌株
CN104498450B (zh) * 2014-12-18 2017-02-22 上海交通大学 褶皱假丝酵母脂肪酶1突变体及其基因
EP3469076B1 (en) * 2016-06-10 2020-09-02 DSM IP Assets B.V. Mutant lipase and use thereof

Also Published As

Publication number Publication date
US20210207181A1 (en) 2021-07-08
EP3397771A2 (en) 2018-11-07
KR20180098386A (ko) 2018-09-03
JP2019500042A (ja) 2019-01-10
CA3009871A1 (en) 2017-07-06
PE20181403A1 (es) 2018-09-07
CN116064263A (zh) 2023-05-05
US11535875B2 (en) 2022-12-27
JP2022046487A (ja) 2022-03-23
WO2017115322A3 (en) 2017-08-10
WO2017115322A2 (en) 2017-07-06
CN108884480A (zh) 2018-11-23

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