BR112018008331B1 - Métodos para uso de amostra - Google Patents

Métodos para uso de amostra Download PDF

Info

Publication number
BR112018008331B1
BR112018008331B1 BR112018008331-3A BR112018008331A BR112018008331B1 BR 112018008331 B1 BR112018008331 B1 BR 112018008331B1 BR 112018008331 A BR112018008331 A BR 112018008331A BR 112018008331 B1 BR112018008331 B1 BR 112018008331B1
Authority
BR
Brazil
Prior art keywords
sample
buffer
swab
illustratively
vial
Prior art date
Application number
BR112018008331-3A
Other languages
English (en)
Portuguese (pt)
Other versions
BR112018008331A2 (pt
Inventor
Stephanie Anne Thatcher
Jeremy Paul Green
Erik Wong Huynh
Andrew Clinton Hemmert
Jesse Linton Montgomery
Robert John Crisp
Kendall A. Rasband
Elizabeth Mary Ott Crowther
Cheryl Lynn Baird
Original Assignee
Biofire Diagnostics, Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biofire Diagnostics, Llc filed Critical Biofire Diagnostics, Llc
Publication of BR112018008331A2 publication Critical patent/BR112018008331A2/pt
Publication of BR112018008331B1 publication Critical patent/BR112018008331B1/pt

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/537—Protease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/153—Viscosity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N2001/028—Sampling from a surface, swabbing, vaporising
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G01N2001/4088—Concentrating samples by other techniques involving separation of suspended solids filtration

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Sampling And Sample Adjustment (AREA)
BR112018008331-3A 2015-11-02 2016-11-01 Métodos para uso de amostra BR112018008331B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562249592P 2015-11-02 2015-11-02
US62/249,592 2015-11-02
PCT/US2016/059928 WO2017079167A1 (en) 2015-11-02 2016-11-01 Sample preparation for difficult sample types

Publications (2)

Publication Number Publication Date
BR112018008331A2 BR112018008331A2 (pt) 2018-10-30
BR112018008331B1 true BR112018008331B1 (pt) 2022-09-06

Family

ID=58635347

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112018008331-3A BR112018008331B1 (pt) 2015-11-02 2016-11-01 Métodos para uso de amostra

Country Status (12)

Country Link
US (2) US11175205B2 (enExample)
EP (2) EP3353278B1 (enExample)
JP (3) JP7126445B2 (enExample)
KR (2) KR20250108762A (enExample)
CN (2) CN115181781A (enExample)
AU (1) AU2016350734B2 (enExample)
BR (1) BR112018008331B1 (enExample)
CA (1) CA3003172A1 (enExample)
CL (1) CL2018001156A1 (enExample)
ES (1) ES2862224T3 (enExample)
SG (1) SG11201802946WA (enExample)
WO (1) WO2017079167A1 (enExample)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9522396B2 (en) 2010-12-29 2016-12-20 S.D. Sight Diagnostics Ltd. Apparatus and method for automatic detection of pathogens
CN104169719B (zh) 2011-12-29 2017-03-08 思迪赛特诊断有限公司 用于检测生物样品中病原体的方法和系统
US20160299132A1 (en) 2013-03-15 2016-10-13 Ancera, Inc. Systems and methods for bead-based assays in ferrofluids
US20160296945A1 (en) 2013-03-15 2016-10-13 Ancera, Inc. Systems and methods for active particle separation
WO2014144340A1 (en) 2013-03-15 2014-09-18 Ancera, Inc. Systems and methods for three-dimensional extraction of target particles ferrofluids
IL227276A0 (en) 2013-07-01 2014-03-06 Parasight Ltd A method and system for preparing a monolayer of cells, particularly suitable for diagnosis
US11285490B2 (en) 2015-06-26 2022-03-29 Ancera, Llc Background defocusing and clearing in ferrofluid-based capture assays
US9933445B1 (en) 2016-05-16 2018-04-03 Hound Labs, Inc. System and method for target substance identification
CN109963640A (zh) * 2016-07-31 2019-07-02 安测络有限责任公司 用于基于铁磁流体的测定的多层一次性盒及其使用方法
US11982611B2 (en) * 2017-03-20 2024-05-14 Nanocellect Biomedical, Inc. Systems, apparatuses, and methods for cell sorting and flow cytometry
JP6823204B2 (ja) 2017-05-24 2021-01-27 バイオファイアー・ディフェンス・エルエルシー アレイのポイント・オブ・ユース排出のためのシステムおよび方法
US20200245898A1 (en) 2019-01-31 2020-08-06 Hound Labs, Inc. Single-use Microfluidic Cartridge for Detection of Target Chemical Presence in Human Breath
US11977086B2 (en) 2019-03-21 2024-05-07 Hound Labs, Inc. Biomarker detection from breath samples
CN110157702A (zh) * 2019-05-24 2019-08-23 中国水产科学研究院南海水产研究所深圳试验基地 一种用于提取珊瑚rna样品的采集操作台
JP7762166B2 (ja) * 2020-05-07 2025-10-29 アボット・ラピッド・ダイアグノスティクス・インターナショナル・アンリミテッド・カンパニー 試料収集および廃棄用スワブ
US11933731B1 (en) 2020-05-13 2024-03-19 Hound Labs, Inc. Systems and methods using Surface-Enhanced Raman Spectroscopy for detecting tetrahydrocannabinol
CN116529371A (zh) * 2020-05-29 2023-08-01 洛克菲勒大学 从自收集小体积样品中分离rna的方法和系统
KR20230018435A (ko) * 2020-05-29 2023-02-07 더 락커펠러 유니버시티 자기-수집된 및 작은 부피 샘플로부터의 rna 단리를 위한 방법 및 시스템
EP4168247A4 (en) * 2020-06-18 2024-06-26 NanoCellect Biomedical, Inc. Microfluidic systems and methods for sorting particles
CN116157076A (zh) * 2020-07-13 2023-05-23 哈佛大学校长及研究员协会 前鼻孔拭子及其用途
CN111822282B (zh) * 2020-07-24 2021-07-20 惠州法路源基因科技有限公司 一种取样拭子植绒设备
US20240002916A1 (en) * 2020-11-23 2024-01-04 Nuclein, Llc Direct lysis apparatus and methods for molecular diagnostics
US12392769B1 (en) 2021-01-12 2025-08-19 Hound Labs, Inc. Ambient contamination in breath analyte detection and measurement
CN113136412A (zh) * 2021-04-22 2021-07-20 浙江珂瑞康生物医疗科技有限公司 用于含可溶性蛋白生物医用材料的生物负载测定处理液
EP4469204A1 (en) * 2022-01-25 2024-12-04 S.D. Sight Diagnostics Ltd. Sample carrier for use with a bodily sample

Family Cites Families (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6645758B1 (en) 1989-02-03 2003-11-11 Johnson & Johnson Clinical Diagnostics, Inc. Containment cuvette for PCR and method of use
JP3784829B2 (ja) 1994-04-14 2006-06-14 ザ ロックフェラー ユニヴァーシティ 細胞成分を単離する方法、装置及び試薬
US6780617B2 (en) 2000-12-29 2004-08-24 Chen & Chen, Llc Sample processing device and method
EP1415113B1 (en) 2001-07-16 2011-08-31 Idaho Technology, Inc. Thermal cycling system and method of use
EP1436405A4 (en) 2001-08-20 2006-01-04 Whatman Inc CLEANING AND OBTAINING DNA FROM HIGH-PARTICLES AND SOLIDS SAMPLES
US20030215845A1 (en) * 2002-02-19 2003-11-20 Bille Todd William Selective extraction of DNA from groups of cells
JP4286157B2 (ja) * 2003-01-21 2009-06-24 デンカ生研株式会社 メンブレンアッセイ法
US20040157219A1 (en) * 2003-02-06 2004-08-12 Jianrong Lou Chemical treatment of biological samples for nucleic acid extraction and kits therefor
EP1654066B1 (en) * 2003-07-31 2014-11-12 Handylab, Inc. Processing particle-containing samples
US7387887B2 (en) 2004-04-20 2008-06-17 University Of Utah Research Foundation Nucleic acid melting analysis with saturation dyes
US8986614B2 (en) * 2010-02-23 2015-03-24 Rheonix, Inc. Self-contained biological assay apparatus, methods, and applications
CA2607661C (en) 2005-05-09 2017-08-08 Idaho Technology, Inc. A device and method for two-stage nucleic acid amplification
US8652782B2 (en) * 2006-09-12 2014-02-18 Longhorn Vaccines & Diagnostics, Llc Compositions and methods for detecting, identifying and quantitating mycobacterial-specific nucleic acids
US9481912B2 (en) 2006-09-12 2016-11-01 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for detecting and identifying nucleic acid sequences in biological samples
JP4297148B2 (ja) 2006-09-22 2009-07-15 ソニー株式会社 核酸回収装置及び核酸回収方法
ES2809168T3 (es) 2006-11-15 2021-03-03 Biofire Diagnostics Llc Análisis biológico autónomo de alta densidad
JP4522434B2 (ja) 2007-05-31 2010-08-11 キヤノン株式会社 血液採取用容器
AU2009281729A1 (en) * 2008-08-15 2010-02-18 Litmus Rapid-B Llc Flow cytometry-based systems and methods for detecting microbes
EP2210936A1 (en) 2009-01-27 2010-07-28 Curetis AG Processing and analysis of viscous liquid biological samples
JP5782384B2 (ja) * 2009-02-03 2015-09-24 ネットバイオ・インコーポレーテッドＮｅｔＢｉｏ， Ｉｎｃ． 未処理試料から核酸を単離するための自己完結型装置および精製方法
US9347086B2 (en) * 2009-04-03 2016-05-24 Integrated Nano-Technologies, Llc Method and system for sample preparation
WO2010147989A1 (en) * 2009-06-15 2010-12-23 Ibis Biosciences, Inc. Compositions and methods for the isolation of nucleic acid
BR112013002064B1 (pt) * 2010-07-27 2020-11-24 Northwestern University Dispositivo e metodos para filtragem do plasma sanguíneo
WO2012027302A2 (en) * 2010-08-21 2012-03-01 The Regents Of The University Of California Systems and methods for detecting antibiotic resistance
WO2012050787A1 (en) * 2010-09-29 2012-04-19 Ibis Biosciences, Inc. Fungal nucleic acid extraction
IT1403618B1 (it) 2011-01-05 2013-10-31 Copan Italia Spa Procedimento per realizzare un dispositivo per il prelievo ed il trasferimento di campioni per biologia molecolare
US8894946B2 (en) * 2011-10-21 2014-11-25 Integenx Inc. Sample preparation, processing and analysis systems
US10865440B2 (en) * 2011-10-21 2020-12-15 IntegenX, Inc. Sample preparation, processing and analysis systems
CA2854768C (en) 2011-11-10 2021-08-17 David E. Jones Loading vials
ES2746565T3 (es) 2012-04-05 2020-03-06 Tauns Co Ltd Procedimiento y kit para la detección inmunológica de complejo de Mycobacterium tuberculosis
WO2013159116A1 (en) * 2012-04-20 2013-10-24 University Of Chicago Fluidic devices for biospecimen preservation
DK2855692T3 (da) 2012-05-24 2019-10-14 Univ Utah Res Found Ekstrem pcr
EP2684947A1 (en) * 2012-07-09 2014-01-15 Biocartis SA A method for filtering a biological sample
AU2013312191C1 (en) 2012-09-10 2017-10-19 Biofire Diagnostics, Llc Multiple amplification cycle detection
WO2014144174A1 (en) 2013-03-15 2014-09-18 Abbott Molecular Inc. Compositions and methods for nucleic acid extraction
US20160376581A1 (en) * 2013-07-02 2016-12-29 Ibis Biosciences, Inc. Method for the purification of targeted nucleic acids from background nucleic acids
JP6318239B2 (ja) 2014-04-24 2018-04-25 株式会社ヤクルト本社 真菌核酸の抽出方法
WO2015187849A2 (en) * 2014-06-04 2015-12-10 Lucigen Corporation Sample collection and analysis devices

Also Published As

Publication number Publication date
US20170122851A1 (en) 2017-05-04
JP7821137B2 (ja) 2026-02-26
EP3353278A1 (en) 2018-08-01
JP2023085281A (ja) 2023-06-20
CN108473931B (zh) 2022-07-19
JP7126445B2 (ja) 2022-08-26
SG11201802946WA (en) 2018-05-30
HK1252952A1 (en) 2019-06-06
EP3789503B1 (en) 2024-04-10
JP2022008815A (ja) 2022-01-14
JP2019502098A (ja) 2019-01-24
EP3789503C0 (en) 2024-04-10
EP3789503A1 (en) 2021-03-10
EP3353278A4 (en) 2019-03-06
WO2017079167A1 (en) 2017-05-11
KR102830890B1 (ko) 2025-07-07
US11175205B2 (en) 2021-11-16
KR20180080241A (ko) 2018-07-11
AU2016350734B2 (en) 2021-04-08
KR20250108762A (ko) 2025-07-15
ES2862224T3 (es) 2021-10-07
AU2016350734A1 (en) 2018-06-14
CN115181781A (zh) 2022-10-14
CA3003172A1 (en) 2017-05-11
US20220034770A1 (en) 2022-02-03
BR112018008331A2 (pt) 2018-10-30
EP3353278B1 (en) 2021-03-10
CN108473931A (zh) 2018-08-31
CL2018001156A1 (es) 2018-11-23

Similar Documents

Publication Publication Date Title
JP7821137B2 (ja) 扱いにくい試料タイプのための試料調製
JP5782384B2 (ja) 未処理試料から核酸を単離するための自己完結型装置および精製方法
CN116724122A (zh) 用于浓缩和鉴定血液中微生物的系统、方法和设备
AU2017235971B2 (en) Nucleic Acid Purification
JP2025525592A (ja) 細胞溶解及び核酸回収
HK1252952B (en) Sample preparation for difficult sample types

Legal Events

Date Code Title Description
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 01/11/2016, OBSERVADAS AS CONDICOES LEGAIS