BR112017018885A2 - método de cultura celular para células-tronco mesenquimais - Google Patents
método de cultura celular para células-tronco mesenquimaisInfo
- Publication number
- BR112017018885A2 BR112017018885A2 BR112017018885A BR112017018885A BR112017018885A2 BR 112017018885 A2 BR112017018885 A2 BR 112017018885A2 BR 112017018885 A BR112017018885 A BR 112017018885A BR 112017018885 A BR112017018885 A BR 112017018885A BR 112017018885 A2 BR112017018885 A2 BR 112017018885A2
- Authority
- BR
- Brazil
- Prior art keywords
- cell culture
- stem cells
- mesenchymal stem
- culture method
- compositions
- Prior art date
Links
- 238000004113 cell culture Methods 0.000 title abstract 2
- 210000002901 mesenchymal stem cell Anatomy 0.000 title 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 abstract 1
- 239000006143 cell culture medium Substances 0.000 abstract 1
- 230000004663 cell proliferation Effects 0.000 abstract 1
- 239000012091 fetal bovine serum Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/99—Serum-free medium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/11—Epidermal growth factor [EGF]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/135—Platelet-derived growth factor [PDGF]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/11—Coculture with; Conditioned medium produced by blood or immune system cells
- C12N2502/115—Platelets, megakaryocytes
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Rheumatology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2015900752A AU2015900752A0 (en) | 2015-03-04 | Cell culture method | |
| AU2015900777A AU2015900777A0 (en) | 2015-03-05 | Cell culture method | |
| PCT/EP2016/054640 WO2016139340A1 (en) | 2015-03-04 | 2016-03-04 | Cell culture method for mesenchymal stem cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112017018885A2 true BR112017018885A2 (pt) | 2018-04-17 |
Family
ID=55453191
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112017018885A BR112017018885A2 (pt) | 2015-03-04 | 2016-03-04 | método de cultura celular para células-tronco mesenquimais |
Country Status (10)
| Country | Link |
|---|---|
| US (4) | US20180037867A1 (https=) |
| EP (2) | EP3265554B1 (https=) |
| JP (3) | JP6885870B2 (https=) |
| KR (1) | KR102635212B1 (https=) |
| CN (2) | CN107567494A (https=) |
| AU (2) | AU2016227607B2 (https=) |
| BR (1) | BR112017018885A2 (https=) |
| CA (1) | CA2977578C (https=) |
| SG (1) | SG11201706886PA (https=) |
| WO (1) | WO2016139340A1 (https=) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2982461T3 (es) | 2008-09-16 | 2024-10-16 | Mayo Found Medical Education & Res | Composiciones que contienen contenido plaquetario |
| US20120087933A1 (en) | 2010-10-08 | 2012-04-12 | Samson Tom | Enhanced msc preparations |
| US11891620B2 (en) | 2014-05-16 | 2024-02-06 | Mayo Foundation For Medical Education And Research | Cell culture media compositions for primary cells |
| CN107567494A (zh) | 2015-03-04 | 2018-01-09 | 迈索布拉斯特国际有限公司 | 间充质干细胞的细胞培养方法 |
| CN119033819A (zh) | 2017-11-22 | 2024-11-29 | 迈索布拉斯特国际有限公司 | 细胞组合物及治疗方法 |
| CN108251358B (zh) * | 2017-12-15 | 2021-10-26 | 广东药科大学 | 一种相同供体来源人间充质干细胞的多批次原代分离方法 |
| SG11202009393QA (en) * | 2018-03-28 | 2020-10-29 | Pluristem Ltd | Methods for expanding adherent stromal cells and cells obtained thereby |
| CN108624554B (zh) * | 2018-05-14 | 2021-09-14 | 山东大学 | 一种非基因修饰光操控双向调节人脐带间充质干细胞增殖的方法 |
| US11364323B2 (en) | 2018-09-17 | 2022-06-21 | Rejuvablast LLC | Combination grafts for tissue repair or regeneration applications |
| WO2020067443A1 (ja) * | 2018-09-27 | 2020-04-02 | テルモ株式会社 | 体細胞のシート化方法 |
| BR112022024866A2 (pt) * | 2020-06-11 | 2023-02-14 | Mesoblast Int Sarl | Cultura 3d de células precursoras da linhagem mesenquimal ou células-tronco |
| CN117396077A (zh) * | 2021-03-10 | 2024-01-12 | 寺崎生物医学创新研究所 | 用于实验室生长肉和其他应用的生长因子 |
| WO2022192429A1 (en) * | 2021-03-10 | 2022-09-15 | Terasaki Insutitute For Biomedical Innovation | Constructs comprising fibrin or other blood products for meat cultivation and other applications |
| EP4630017A1 (en) | 2022-12-09 | 2025-10-15 | Mesoblast International Sàrl | Method of treating heart failure in subjects with persistent inflammation |
| AU2023389701A1 (en) | 2022-12-09 | 2025-07-10 | Mesoblast International Sarl | Method of treating inflammation with cellular compositions |
| AU2023389702A1 (en) | 2022-12-09 | 2025-07-10 | Mesoblast International Sarl | Pre-licensed composition and cell culture methods |
| CN120603930A (zh) | 2022-12-09 | 2025-09-05 | 迈索布拉斯特国际有限公司 | 条件培养基及其用途 |
| WO2024225602A1 (ko) * | 2023-04-24 | 2024-10-31 | 파미셀 주식회사 | 뉴클레오시드 함유 세포 배양배지 및 이를 이용한 세포 배양 방법 |
| AU2024332651A1 (en) | 2023-08-28 | 2026-04-02 | Mesoblast International Sàrl | Third line treatment for gvhd |
| WO2025062365A1 (en) | 2023-09-22 | 2025-03-27 | Mesoblast International Sárl | Mesenchymal lineage precursor or stem cells (mlpscs) for use in methods of treating congenital heart disease |
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| US5486359A (en) | 1990-11-16 | 1996-01-23 | Osiris Therapeutics, Inc. | Human mesenchymal stem cells |
| WO1993003679A1 (de) | 1991-08-12 | 1993-03-04 | Karl Storz Gmbh & Co. | Hochfrequenzchirurgiegenerator zum geregelten schneiden und koagulieren |
| US5814464A (en) | 1994-10-07 | 1998-09-29 | Regeneron Pharma | Nucleic acids encoding TIE-2 ligand-2 |
| US6277372B1 (en) | 1994-11-08 | 2001-08-21 | Diacrin, Inc. | Porcine neural cells and their use in treatment of neurological deficits due to neurodegenerative diseases |
| US6204053B1 (en) | 1994-11-08 | 2001-03-20 | Diacrin, Inc. | Porcine cortical cells and their use in treatment of neurological deficits due to neurodegenerative diseases |
| WO1996031598A1 (en) | 1995-04-06 | 1996-10-10 | Regeneron Pharmaceuticals, Inc. | Tie-2 ligands, methods of making and uses thereof |
| CA2277278A1 (en) | 1997-01-10 | 1998-07-16 | Life Technologies, Inc. | Embryonic stem cell serum replacement |
| US5968829A (en) | 1997-09-05 | 1999-10-19 | Cytotherapeutics, Inc. | Human CNS neural stem cells |
| JP2002529071A (ja) * | 1998-11-09 | 2002-09-10 | コンソルツィオ・ペール・ラ・ジェスティオーネ・デル・チェントロ・ディ・ビオテクノロジー・アヴァンツァテ | 軟骨細胞様細胞のための無血清培地 |
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| AU2118501A (en) | 1999-10-20 | 2001-04-30 | Stemcells, Inc. | Methods for inducing in vivo proliferation and migration of transplanted progenitor cells in the brain |
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| EP2316918B1 (en) | 2001-02-14 | 2015-07-01 | Anthrogenesis Corporation | Post-partum mammalian placenta, its use and placental stem cells therefrom |
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| NZ534643A (en) | 2002-02-13 | 2010-06-25 | Anthrogenesis Corp | Embryonic-like stem cells derived from post-partum mammalian placenta and uses and methods of treatment using said cells |
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| JP2008148643A (ja) * | 2006-12-19 | 2008-07-03 | Olympus Corp | 無血清培地および幹細胞の培養方法 |
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| KR101224588B1 (ko) * | 2008-01-24 | 2013-01-22 | 삼성전자주식회사 | 멀티포인트 스트록을 감지하기 위한 ｕｉ 제공방법 및 이를적용한 멀티미디어 기기 |
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| US20100055078A1 (en) * | 2008-08-15 | 2010-03-04 | The Government of the U.S.A, as represented by the Department of Veterans Affairs | Methods of making a transplantable bone repair system using multipotent stem cells |
| KR101712501B1 (ko) | 2008-11-11 | 2017-03-07 | 고쿠리츠다이가쿠호진 히로시마다이가쿠 | 분화 유도 배지용 첨가제 및 그의 용도 |
| US20100158878A1 (en) | 2008-12-23 | 2010-06-24 | Alexandra Capela | Target populations of oligodendrocyte precursor cells and methods of making and using same |
| US10894944B2 (en) | 2009-04-10 | 2021-01-19 | Monash University | Cell culture media |
| CA2792802C (en) * | 2010-03-10 | 2017-10-31 | Two Cells Co., Ltd. | Cell preparation containing mesenchymal stem cells, and method for producing same |
| CN103068969A (zh) * | 2010-06-17 | 2013-04-24 | 思迪公司 | 化学成分确定的无血清细胞培养基 |
| TWI586805B (zh) * | 2011-01-12 | 2017-06-11 | 城戶常雄 | 獲得及維持在體外易於分化為寡樹突細胞－族系細胞之哺乳類神經幹細胞及／或神經祖源細胞的純或增富族群之培養方法 |
| KR101523040B1 (ko) * | 2012-06-26 | 2015-05-26 | 라정찬 | 고농도의 줄기세포 제조방법 |
| WO2014082685A1 (en) * | 2012-11-30 | 2014-06-05 | Swiss Stem Cell Foundation | Serum-free medium for human mesenchymal stem cells |
| CN103396984B (zh) * | 2013-07-25 | 2016-02-10 | 宗兆文 | 一种利用皮肤来源的祖细胞制备组织工程脊髓的方法 |
| CN107567494A (zh) | 2015-03-04 | 2018-01-09 | 迈索布拉斯特国际有限公司 | 间充质干细胞的细胞培养方法 |
-
2016
- 2016-03-04 CN CN201680025469.8A patent/CN107567494A/zh active Pending
- 2016-03-04 EP EP16707810.4A patent/EP3265554B1/en active Active
- 2016-03-04 CN CN202111251410.XA patent/CN113957043A/zh active Pending
- 2016-03-04 AU AU2016227607A patent/AU2016227607B2/en active Active
- 2016-03-04 CA CA2977578A patent/CA2977578C/en active Active
- 2016-03-04 EP EP21205011.6A patent/EP4008775A1/en active Pending
- 2016-03-04 WO PCT/EP2016/054640 patent/WO2016139340A1/en not_active Ceased
- 2016-03-04 SG SG11201706886PA patent/SG11201706886PA/en unknown
- 2016-03-04 KR KR1020177025840A patent/KR102635212B1/ko active Active
- 2016-03-04 JP JP2017546094A patent/JP6885870B2/ja active Active
- 2016-03-04 US US15/553,115 patent/US20180037867A1/en not_active Abandoned
- 2016-03-04 BR BR112017018885A patent/BR112017018885A2/pt not_active Application Discontinuation
-
2019
- 2019-10-11 US US16/599,271 patent/US11685899B2/en active Active
-
2021
- 2021-03-16 JP JP2021042409A patent/JP7410899B2/ja active Active
- 2021-10-19 AU AU2021254538A patent/AU2021254538B2/en active Active
-
2023
- 2023-03-21 US US18/124,346 patent/US12215352B2/en active Active
- 2023-10-05 JP JP2023173585A patent/JP2024001179A/ja active Pending
-
2024
- 2024-12-24 US US19/001,125 patent/US20250129337A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CA2977578A1 (en) | 2016-09-09 |
| AU2016227607B2 (en) | 2021-09-30 |
| SG11201706886PA (en) | 2017-09-28 |
| JP2021090463A (ja) | 2021-06-17 |
| JP2024001179A (ja) | 2024-01-09 |
| EP3265554B1 (en) | 2021-11-17 |
| WO2016139340A1 (en) | 2016-09-09 |
| US20200040303A1 (en) | 2020-02-06 |
| AU2016227607A1 (en) | 2017-09-07 |
| US20250129337A1 (en) | 2025-04-24 |
| US12215352B2 (en) | 2025-02-04 |
| AU2021254538A1 (en) | 2021-11-18 |
| JP7410899B2 (ja) | 2024-01-10 |
| US20180037867A1 (en) | 2018-02-08 |
| KR20170120627A (ko) | 2017-10-31 |
| AU2021254538B2 (en) | 2024-01-18 |
| US20240191200A1 (en) | 2024-06-13 |
| EP3265554A1 (en) | 2018-01-10 |
| JP2018506986A (ja) | 2018-03-15 |
| KR102635212B1 (ko) | 2024-02-13 |
| US11685899B2 (en) | 2023-06-27 |
| EP4008775A1 (en) | 2022-06-08 |
| CN107567494A (zh) | 2018-01-09 |
| CN113957043A (zh) | 2022-01-21 |
| CA2977578C (en) | 2024-09-10 |
| JP6885870B2 (ja) | 2021-06-16 |
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