CA2977578C - Cell culture method for mesenchymal stem cells - Google Patents

Cell culture method for mesenchymal stem cells

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Publication number
CA2977578C
CA2977578C CA2977578A CA2977578A CA2977578C CA 2977578 C CA2977578 C CA 2977578C CA 2977578 A CA2977578 A CA 2977578A CA 2977578 A CA2977578 A CA 2977578A CA 2977578 C CA2977578 C CA 2977578C
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Prior art keywords
pdgf
cell culture
culture medium
level
fgf2
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CA2977578A
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English (en)
French (fr)
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CA2977578A1 (en
Inventor
Paul Simmons
Colby SUIRE
Original Assignee
Mesoblast International SARL
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Priority claimed from AU2015900752A external-priority patent/AU2015900752A0/en
Application filed by Mesoblast International SARL filed Critical Mesoblast International SARL
Publication of CA2977578A1 publication Critical patent/CA2977578A1/en
Application granted granted Critical
Publication of CA2977578C publication Critical patent/CA2977578C/en
Active legal-status Critical Current
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/99—Serum-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/135—Platelet-derived growth factor [PDGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/11—Coculture with; Conditioned medium produced by blood or immune system cells
    • C12N2502/115—Platelets, megakaryocytes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Rheumatology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CA2977578A 2015-03-04 2016-03-04 Cell culture method for mesenchymal stem cells Active CA2977578C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
AU2015900752A AU2015900752A0 (en) 2015-03-04 Cell culture method
AU2015900752 2015-03-04
AU2015900777A AU2015900777A0 (en) 2015-03-05 Cell culture method
AU2015900777 2015-03-05
PCT/EP2016/054640 WO2016139340A1 (en) 2015-03-04 2016-03-04 Cell culture method for mesenchymal stem cells

Publications (2)

Publication Number Publication Date
CA2977578A1 CA2977578A1 (en) 2016-09-09
CA2977578C true CA2977578C (en) 2024-09-10

Family

ID=55453191

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2977578A Active CA2977578C (en) 2015-03-04 2016-03-04 Cell culture method for mesenchymal stem cells

Country Status (10)

Country Link
US (4) US20180037867A1 (https=)
EP (2) EP4008775A1 (https=)
JP (3) JP6885870B2 (https=)
KR (1) KR102635212B1 (https=)
CN (2) CN107567494A (https=)
AU (2) AU2016227607B2 (https=)
BR (1) BR112017018885A2 (https=)
CA (1) CA2977578C (https=)
SG (1) SG11201706886PA (https=)
WO (1) WO2016139340A1 (https=)

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US20210115395A1 (en) * 2018-03-28 2021-04-22 Pluristem Ltd. Methods for expanding adherent stromal cells and cells obtained thereby
CN108624554B (zh) * 2018-05-14 2021-09-14 山东大学 一种非基因修饰光操控双向调节人脐带间充质干细胞增殖的方法
US11364323B2 (en) 2018-09-17 2022-06-21 Rejuvablast LLC Combination grafts for tissue repair or regeneration applications
WO2020067443A1 (ja) * 2018-09-27 2020-04-02 テルモ株式会社 体細胞のシート化方法
JP2023530098A (ja) * 2020-06-11 2023-07-13 メゾブラスト・インターナショナル・エスアーエールエル 間葉系前駆細胞又は幹細胞の３ｄ培養
CN117396077A (zh) * 2021-03-10 2024-01-12 寺崎生物医学创新研究所 用于实验室生长肉和其他应用的生长因子
US20240268422A1 (en) * 2021-03-10 2024-08-15 Terasaki Institute For Biomedical Innovation Methods and systems of preparing cultivated meat from blood or cellular biomass
WO2024121818A1 (en) 2022-12-09 2024-06-13 Mesoblast International Sarl Method of treating heart failure in subjects with persistent inflammation
KR20250130613A (ko) 2022-12-09 2025-09-02 메조블라스트 인터내셔널 에스에이알엘 세포 조성물을 이용한 염증 치료 방법
JP2025538784A (ja) 2022-12-09 2025-11-28 メゾブラスト・インターナショナル・エスアーエールエル 馴化培地及びその使用
KR20250133306A (ko) 2022-12-09 2025-09-05 메조블라스트 인터내셔널 에스에이알엘 사전 허가된 조성물 및 세포 배양 방법
WO2024225602A1 (ko) * 2023-04-24 2024-10-31 파미셀 주식회사 뉴클레오시드 함유 세포 배양배지 및 이를 이용한 세포 배양 방법
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Also Published As

Publication number Publication date
SG11201706886PA (en) 2017-09-28
JP6885870B2 (ja) 2021-06-16
CN113957043A (zh) 2022-01-21
JP2018506986A (ja) 2018-03-15
AU2021254538B2 (en) 2024-01-18
WO2016139340A1 (en) 2016-09-09
US20240191200A1 (en) 2024-06-13
EP4008775A1 (en) 2022-06-08
CN107567494A (zh) 2018-01-09
EP3265554A1 (en) 2018-01-10
US20200040303A1 (en) 2020-02-06
AU2016227607B2 (en) 2021-09-30
US12215352B2 (en) 2025-02-04
BR112017018885A2 (pt) 2018-04-17
US11685899B2 (en) 2023-06-27
CA2977578A1 (en) 2016-09-09
AU2016227607A1 (en) 2017-09-07
US20250129337A1 (en) 2025-04-24
AU2021254538A1 (en) 2021-11-18
EP3265554B1 (en) 2021-11-17
JP2024001179A (ja) 2024-01-09
JP2021090463A (ja) 2021-06-17
JP7410899B2 (ja) 2024-01-10
KR102635212B1 (ko) 2024-02-13
KR20170120627A (ko) 2017-10-31
US20180037867A1 (en) 2018-02-08

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