BR112017003558A2 - método de cultivo de um micro-organismo. - Google Patents

método de cultivo de um micro-organismo.

Info

Publication number
BR112017003558A2
BR112017003558A2 BR112017003558-8A BR112017003558A BR112017003558A2 BR 112017003558 A2 BR112017003558 A2 BR 112017003558A2 BR 112017003558 A BR112017003558 A BR 112017003558A BR 112017003558 A2 BR112017003558 A2 BR 112017003558A2
Authority
BR
Brazil
Prior art keywords
volume
crop
medium
microorganisms
microorganism
Prior art date
Application number
BR112017003558-8A
Other languages
English (en)
Other versions
BR112017003558B1 (pt
Inventor
Berryman Kevin
Sun Zhiyong
Milway Michael
VALENTINE Mercia
E. ARMENTA Roberto
Original Assignee
MARA Renewables Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by MARA Renewables Corporation filed Critical MARA Renewables Corporation
Publication of BR112017003558A2 publication Critical patent/BR112017003558A2/pt
Publication of BR112017003558B1 publication Critical patent/BR112017003558B1/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06Quantitative determination
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04Preserving or maintaining viable microorganisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/10Protozoa; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/64Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409Fatty acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/64Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409Fatty acids
    • C12P7/6427Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Toxicology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fodder In General (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Cultivation Of Plants (AREA)

Abstract

são fornecidos neste documento métodos de cultivo de um micro-organismo. o método inclui o fornecimento de um recipiente compreendendo um ou mais micro-organismos e um meio, em que os micro-organismos e o meio formam um volume inicial. os micro-organismos e o meio são cultivados até que a cultura atinja um indicador de limite, em que o cultivo compreende a introdução de uma ou mais fontes de carbono à cultura e em que a cultura está em um volume limite quando o indicador de limite é atingido. o método também inclui a colheita de uma porção do volume limite para deixar um volume residual que seja 40% ou menos do volume inicial e adição de meio fresco ao recipiente numa quantidade para retornar o volume da cultura ao volume inicial.
BR112017003558-8A 2014-10-16 2015-10-12 Método de repetição de batelada alimentada de cultivo de um microorganismo BR112017003558B1 (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201462064694P 2014-10-16 2014-10-16
US62/064,694 2014-10-16
US62/064694 2014-10-16
PCT/IB2015/057808 WO2016059541A1 (en) 2014-10-16 2015-10-12 Repeated fed-batch culture methods

Publications (2)

Publication Number Publication Date
BR112017003558A2 true BR112017003558A2 (pt) 2018-02-27
BR112017003558B1 BR112017003558B1 (pt) 2023-10-03

Family

ID=55746208

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017003558-8A BR112017003558B1 (pt) 2014-10-16 2015-10-12 Método de repetição de batelada alimentada de cultivo de um microorganismo

Country Status (14)

Country Link
US (3) US10920261B2 (pt)
EP (1) EP3207165B1 (pt)
JP (1) JP6773301B2 (pt)
KR (1) KR102501032B1 (pt)
CN (2) CN106604998B (pt)
AU (2) AU2015332095B2 (pt)
BR (1) BR112017003558B1 (pt)
CA (1) CA2960450C (pt)
CL (1) CL2017000892A1 (pt)
DK (1) DK3207165T3 (pt)
ES (1) ES2785331T3 (pt)
IL (1) IL250269A0 (pt)
MX (1) MX2017002150A (pt)
WO (1) WO2016059541A1 (pt)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109136308B (zh) * 2018-09-26 2021-01-26 武汉中科光谷绿色生物技术有限公司 一种提高发酵产聚唾液酸的方法及发酵液
CN109161570B (zh) * 2018-09-26 2020-12-18 武汉中科光谷绿色生物技术有限公司 一种提高发酵产n-乙酰神经氨酸的方法及发酵液

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5340742A (en) 1988-09-07 1994-08-23 Omegatech Inc. Process for growing thraustochytrium and schizochytrium using non-chloride salts to produce a microfloral biomass having omega-3-highly unsaturated fatty acids
US5340594A (en) 1988-09-07 1994-08-23 Omegatech Inc. Food product having high concentrations of omega-3 highly unsaturated fatty acids
EP2341126A3 (en) * 2000-01-28 2011-10-05 Martek Biosciences Corporation Enhanced production of lipids containing polyenoic fatty acids by high density cultures of eukaryotic microbes in fermentors
US20070015263A1 (en) * 2003-09-01 2007-01-18 Mogens Wumpelmann Method for increasing yield of biomass of and/or components of biomass from marine microorganisms
EP2447356B1 (en) * 2005-06-07 2016-04-20 DSM Nutritional Products AG Eukaryotic microorganisms for producing lipids and antioxidants
MX2009001346A (es) * 2006-08-01 2009-04-17 Ocean Nutrition Canada Ltd Microbios productores de aceite y metodos de modificacion de los mismos.
US20100124583A1 (en) * 2008-04-30 2010-05-20 Xyleco, Inc. Processing biomass
EP2071019A1 (en) * 2007-12-15 2009-06-17 Lonza AG Method for the cultivation of microoranisms of the order thraustochytriales
US20110306102A1 (en) * 2009-02-25 2011-12-15 V. B. Medicare Pvt. Ltd. Improved methods for fermentative production of docosahexaenoic acid
CA2769003C (en) 2009-07-23 2018-10-16 Xcellerex, Inc. Drain down and re-feed of microcarrier bioreactor
CN107630017A (zh) * 2011-03-07 2018-01-26 Dsm营养产品股份公司 工程化破囊壶菌属微生物
JP6174569B2 (ja) * 2012-03-26 2017-08-02 Bio−energy株式会社 エタノールの生産方法
CN103436561B (zh) * 2013-09-05 2014-11-26 江南大学 以棉纤维材料固定琥珀酸放线杆菌发酵生产丁二酸的方法

Also Published As

Publication number Publication date
ES2785331T3 (es) 2020-10-06
KR20170067748A (ko) 2017-06-16
AU2015332095A1 (en) 2017-02-16
CA2960450C (en) 2023-02-14
KR102501032B1 (ko) 2023-02-16
IL250269A0 (en) 2017-03-30
AU2021202832A1 (en) 2021-06-03
US20160108453A1 (en) 2016-04-21
EP3207165B1 (en) 2020-02-05
JP6773301B2 (ja) 2020-10-21
EP3207165A4 (en) 2018-06-06
CN112961785A (zh) 2021-06-15
US11827918B2 (en) 2023-11-28
EP3207165A1 (en) 2017-08-23
AU2015332095B2 (en) 2021-02-18
JP2017532022A (ja) 2017-11-02
US10920261B2 (en) 2021-02-16
CL2017000892A1 (es) 2017-11-03
CN106604998B (zh) 2021-03-30
BR112017003558B1 (pt) 2023-10-03
US20210123083A1 (en) 2021-04-29
CN106604998A (zh) 2017-04-26
AU2021202832B2 (en) 2023-08-24
US20240060112A1 (en) 2024-02-22
MX2017002150A (es) 2017-08-21
WO2016059541A1 (en) 2016-04-21
CA2960450A1 (en) 2016-04-21
DK3207165T3 (da) 2020-04-06

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Legal Events

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B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 12/10/2015, OBSERVADAS AS CONDICOES LEGAIS